The objective of the present study is to investigate the effect of solvent type (methanol, acetone, ethanol, n-propanol, and water), solvent-to-solid ratio (20–100 mL/g), solvent acidity (0–1.25% HCl), extraction time (60–300 min) and extraction temperature (30–70 °C) on total phenolic content (TPC), total flavonoid content (TFC) and the free radical-scavenging capacity (2,2′-diphenyl-1-picrylhydrazyl (DPPH) and ABTS radical scavenging assay) of koreeb (Dactyloctenium aegyptium) seeds using single-factor experiments. On the basis of TPC and antioxidant activity assays, the best extraction conditions were 80 mL methanol/g, 1.00% HCl, 180 min and 60 °C, as well as the results of TPC, TFC and DPPH were 32.38 mg GAE/g, 20.88 mg QE/g and 82.22%, respectively. The TPC was positively observed to be correlated with TFC, DPPH and ATBS (r = 0.89, 70 and 0.66, respectively) of koreeb seeds flour (KSF) extracts, under influence of solvent type extraction, whereas TPC and TFC were significantly correlated with DPPH (0.97 ≥ r ≥ 0.94) and ABTS (0.95 ≥ r ≥ 0.94) under the effect of extraction temperature. In general, KSF can be applied in functional foods and medicine related products for its health benefits.