Seed Treatment Research Articles

Effect of Micronutrient Seed Treatments on Nutrient Uptake by Bt Cotton in Vertisol

The present investigation was carried out during the year June 2017 to January 2018 at Department of Soil Science and Agricultural Chemistry, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, Maharashtra, India to study the effect of nutrient seed treatments on nutrient uptake, quality of Bt cotton and soil nutrient dynamics in vertisol. The field experiment was undertaken in a Randomized Block Design with nine treatments and three replications. The nutrient seed dressing treatments are absolute control, 100% NPK+Zinc Sulphate (ZnSo4), 100% NPK+Zn EDTA, 100% NPK+Borax (B), 100% NPK+Manganese Sulphate (MnSo4), 100% NPK+Sodium Molybdate (NaMo), 100% NPK+Copper Nitrate (CuNo3), 100% NPK+Ferrous Sulphate (FeSo4), 100% NPK+Fe EDTA seed application to Bt cotton. The maximum N, P, K, Fe, Mn, Zn, Cu, B and Mo uptake was recorded in the treatment 100 % NPK+Zn EDTA followed by 100% NPK+Fe EDTA treatment. In case of Mo uptake, the treatment 100 % NPK+Zn EDTA was significantly superior overall treatments except treatment 100% NPK+Fe EDTA and 100% NPK+Sodium Molybdate (NaMo). The minimum N, P, K, Fe, Mn, Zn, Cu, B and Mo uptake was recorded in the treatment Absolute control+100% NPK.

Effect of Germination on Seed Protein Quality and Secondary Metabolites and Potential Modulation by Pulsed Electric Field Treatment.

Plant-based foods are being increasingly favored to feed the ever-growing population, but these need to exhibit improved nutritional value in terms of protein quality and digestibility to be considered a useful alternative to animal-based foods. Germination is essential for plant growth and represents a viable method through which the protein quality of plants can be further improved. However, it will be a challenge to maintain efficient rates of germination in a changing climate when seeds are sown. In the context of the indoor germination of seeds for food, consumption, or processing purposes, a more efficient and sustainable process is desired. Therefore, novel techniques to facilitate seed germination are required. Pulsed electric fields (PEF) treatment of seeds results in the permeabilization of the cell membrane, allowing water to be taken up more quickly and triggering biochemical changes to the macromolecules in the seed during germination. Therefore, PEF could be a chemical-free approach to induce a stress response in seeds, leading to the production of secondary metabolites known to exert beneficial effects on human health. However, this application of PEF, though promising, requires further research to optimize its impact on the protein and bioactive compounds in germinating seeds.

The effectiveness of using a plant growth stimulator based on Ambrosia artemisiifolia when growing corn in the Kabardino-Balkarian Republic

The article presents the results of studies on the use of a biological product based on ragweed, due to its chemical composition, on corn crops. Double treatment of corn crops with a plant growth stimulator based on ragweed (Ambrosia artemisiifolia L.) was carried out at growth phases of 3–5 and 6–7 leaves in calculated doses with a working solution consumption of 300 l/ha. The repetition in the experiments is 4-fold, the placement of the variants of the experiment is randomized. Plot area – 50 sq.m. The use of a plant growth stimulator based on ragweed as a pre-sowing treatment of seeds and foliar dressings can increase the yield of corn grain by 14–40%, depending on the corn hybrids studied in the experiment. On the variants of the experiment, where the combined use of organic fertilizer Biohumus and plant growth stimulator based on ragweed (solution 3) was studied, the above quality indicators were higher than the values of the control variant by 1.6; 6.0 and 1.5% – in the hybrid Ladoga 191 MV; at 3.2; 3.0 and 3.8% – Krasnodar 196 MV; by 2.9; 5.9 and 2.2% – Darina MV; at 3.3; 6.2 and 3.7% – Kuban. In the variant of the experiment with the joint use of organic fertilizer Biohumus and plant growth stimulator based on ragweed (solution 3), the value of the conditional income indicator decreased by 5400 rubles, according to the variant of the experiment with the joint use of organic fertilizer Biohumus and stimulant growth of plants based on ragweed (solution 4), the value of this indicator decreased by 7200 rubles, relative to the variant of the experiment with the combined use of organic fertilizer Biohumus and plant growth stimulator based on ragweed (solution 1) .

Effect of Treatments of Colchicine, Diethyl Sulphate and Triethylamine in Tomato (<I>Lycopersicon esculentum</I> Mill.)

The present investigation was undertaken in one variety of Tomato, Best of All, to compare the cytogenetic effects of treatments of seeds of tomato with colchicine, diethyl sulphate and triethylamine and also to compare the morphological and physiological effects of these chemicals.Lowest number of seeds germinated from diethyl sulphate treatment. Plants originating from colchicine, diethyl sulphate and triethylamine treatments showed many variant types. Interesting among these were those showing spotting on leaves originating from diethyl sulphate treatment, dwarf ones originating from colchicine and diethyl sulphate treatments and those with abnormal leaves originating from treatment with all the three chemicals.Mitotic irregularities included clumped chromosomes and fragments at metaphase and bridges and fragments at anaphase while in meiosis clumped chromosomes, univalents, trivalents and quadrivalents were noted at metaphase I. Anaphase I irregularities included cells with fragments, bridges with or without fragments, lagging chromosomes and unequal separation of chromosomes. In metaphase II cells with aneuploid chromosome numbers and those with fragments were noticed while in anaphase II cells were found with fragments and unequal separation of chromosomes.The importance of detecting interesting mutants in the Ma generation and the importance of finding translocations and inversions have been pointed out.

Management of Soybean Cyst Nematode and Sudden Death Syndrome with Nematode-Protectant Seed Treatments Across Multiple Environments in Soybean.

As soybean (Glycine max) production continues to expand in the United States and Canada, so do pathogens and pests that directly threaten soybean yield potential and economic returns for farmers. One such pathogen is the soybean cyst nematode (SCN; Heterodera glycines). SCN has traditionally been managed using SCN-resistant cultivars and rotation with nonhost crops, but the interaction of SCN with sudden death syndrome (SDS; caused by Fusarium virguliforme) in the field makes management more difficult. Nematode-protectant seed treatments have become options for SCN and SDS management. The objectives of this study were to evaluate nematode-protectant seed treatments for their effects on (i) early and full season SCN reproduction, (ii) foliar symptoms and root-rot caused by SDS, and (iii) soybean yield across environments accounting for the above factors. Using a standard protocol, field trials were implemented in 13 states and one Canadian province from 2019 to 2021 constituting 51 site-years. Six nematode-protectant seed treatment products were compared with a fungicide + insecticide base treatment and a nontreated check. Initial (at soybean planting) and final (at soybean harvest) SCN egg populations were enumerated, and SCN females were extracted from roots and counted at 30 to 35 days postplanting. Foliar disease index (FDX) and root rot caused by the SDS pathogen were evaluated, and yield data were collected for each plot. No seed treatment offered significant nematode control versus the nontreated check for in-season and full-season nematode response, no matter the initial SCN population or FDX level. Of all treatments, ILEVO (fluopyram) and Saltro (pydiflumetofen) provided more consistent increases in yield over the nontreated check in a broader range of SCN environments, even when FDX level was high.

Seed treatment with essential oils to control Sclerotium rolfsii Sacc

The sclerotia wilt in cowpea caused by the fungus Sclerotium rolfsii has in chemical control an inefficient option. Essential oils have antifungal properties, constituting an alternative to agrochemicals. Here, we evaluated the efficiency of essential oils in the control of S. rolfsii, from seed treatment, as well as their effect on seed germination. Based on the diameter of the colonies of the fungus S. rolfssi submitted to a concentration of 8.0 ml/kg of seed-1, the oils of L. sidoides, L. origanoides and C. zehntneri reduced mycelial growth by 61.5, 67.2 and 40% respectively. The reduction in the percentage of germination occurred at the highest concentrations. The fungus was sensitive to all evaluated oils, being more sensitive to Lippia oils. Seed treatment with essential oils is effective at a concentration of 4.0 ml/kg-1.

Interactive effect of ambient and elevated levels of tropospheric ozone, nutrition and PGPR on growth and yield of chickpea (Cicer arietinum)

An experiment was conducted during 2020 and 2021 at ICAR-Indian Agricultural Research Institute, New Delhi, to study the interactive effect of nutrient and PGPR (Plant growth promoting rhizobacteria) on growth and yield of chickpea (Cicer aeritinum L.) cv. Pusa 3043 under ambient and elevated levels of ozone in FAOE (Free air ozone enrichment). A loss of 15–16% in dry matter production was observed under elevated ozone (EO3) when compared to ambient ozone (AO3) conditions. Seed treatment with PGPR was found to compensate for this loss as an increase in seed yield 18–19% was observed over untreated seeds. In terms of seed yield, the PGPR treatments with 75% nitrogen showed significant improvement compared to the recommended dose of fertilizer (RDF) application. A yield increase of 12% and 14% was observed with PGPR like RPAN8 (Anabena laxa) and An-Rh (Anabena torulosa with Mesorhizobium ciceri) (chickpea rhizobium) respectively, suggesting an advantage of an–Rh over RPAN8 under EO3. A combination of both PGPRs outperformed the individual PGPR, showing a 19% yield increase over RDF. This proves the potential of seed treatment with PGPR (RPAN8 and An-Rh) in ameliorating tropospheric ozone-induced stress in chickpea plants.

Relationship Between Chemical Treatment of Soybean (Glycine max (L.) Merr.) Seeds and Synthesis of Antioxidant Enzymes

This research aimed to assess the relationship between chemical treatment with different combinations of products and the physiological quality of seeds stored for 0 and 180 days. The experimental design adopted was the completely randomized (CRD), with four replications. The treatments consisted of the “cultivar”, “seed treatments” and “storage periods” factors. To characterize the quality of the seeds, lignin quantification analysis, peroxidase activity analysis, catalase activity analysis, and scanning electron microscopy (SEM) were carried out. The physiological quality of the seeds decreases as the storage period increases. The lignin present in the seed coat of cultivar TMG 7061 IPRO provided greater protection compared to cultivar 7067. The images referring to cultivar 7067 showed morphological changes resulting from damage caused by the treatment and storage of the seeds.

Relationship Between Chemical Treatment of Soybean (Glycine max (L.) Merr.) Seeds and Synthesis of Antioxidant Enzymes

This research aimed to assess the relationship between chemical treatment with different combinations of products and the physiological quality of seeds stored for 0 and 180 days. The experimental design adopted was the completely randomized (CRD), with four replications. The treatments consisted of the “cultivar”, “seed treatments” and “storage periods” factors. To characterize the quality of the seeds, lignin quantification analysis, peroxidase activity analysis, catalase activity analysis, and scanning electron microscopy (SEM) were carried out. The physiological quality of the seeds decreases as the storage period increases. The lignin present in the seed coat of cultivar TMG 7061 IPRO provided greater protection compared to cultivar 7067. The images referring to cultivar 7067 showed morphological changes resulting from damage caused by the treatment and storage of the seeds.

Effective biological control of chickpea rabies (Ascochyta rabiei) through systemic phytochemical defenses activation by Trichoderma roots colonization: From strain characterization to seed coating

Chickpea (Cicer arietinum) is a legume of great economic and agricultural importance worldwide, whose crop is severely affected by rust or Ascochyta blight, caused by the fungus Ascochyta rabiei. The fungal genus Trichoderma includes several species widely characterized as effective biological control agents against crop pathogens. First, this work characterized several species of the genus Trichoderma as potential biological control agents of A. rabiei directly (in vitro confrontation) or indirectly in chickpea plants (activation of systemic resistance), selecting T. harzianum EN1 as the most efficient strain. Subsequently, different materials were tested as coatings to apply the T. harzianum conidia on chickpea seeds, determining that gum arabic at 1 % concentration was the one that most promoted the germination of conidia and seeds. The third phase of the study was based on the application of the coating and T. harzianum conidia on chickpea seeds and to study plant survival after infection with the pathogen A. rabiei, characterizing root colonization by Trichoderma and systemic hormonal and metabolic changes related to the induction of systemic defenses. The treatment of chickpea seeds with gum arabic and T. harzianum conidia was found to increase Trichoderma-root colonization and to improve plant survival. The induction of systemic ethylene- and melatonin-mediated resistance, which led to the accumulation of nicotinic acid in plant tissues, was considered the cause of such a protection. Therefore, T. harzianum applied as seed coating with gum arabic could be a good biological control strategy against A. rabiei on chickpea, due to the induction of systemic resistance.

Восстановление плодородия малопродуктивных дерново-подзолистых почв, вновь вводимых в сельскохозяйственный оборот

The purpose of research is to develop agro-reclamation measures to restore the fertility of low-productive sod-podzolic sandy loam soils for the conditions of the southern part of the Non-Chernozem Zone of Russia. Objectives: to conduct a comparative assessment of the impact of granular fertilizer based on turkey droppings and a liquid-phase biological product (LPBP) on increasing soil fertility, phenological characteristics of plants, crop yields and their quality. Six variants of the field experiment were developed: control variant (without fertilizers); granular fertilizer based on turkey droppings at a dose of 15 t/ha; granular fertilizer based on turkey manure at a dose of 15 t/ha together with pre-sowing seed treatment with 1 % LPBP; pre-sowing seed treatment with 1 % LPBP; granular fertilizer based on turkey droppings at a dose of 30 t/ha. The application of organic ameliorant and pre-sowing treatment of seeds with a biological pre¬paration help to improve the agrochemical parameters of the soil, and, as a result, a higher yield of perennial grasses. The best option is option G30, where the yield was 48.3 t/ha of green mass of grass in total for 2 cuttings, which is 33.8 % more than the control option. In the G15 and G15 LPBP options, the harvest was practically not inferior and exceeded the control option by 30.5 and 24.4 %, respectively, soil fertility indicators improved: soil acidity decreased to 4.8–5.0 (pHsol) and 5.4–5.6 (pHwod), an increase in organic matter by 33.9 %, the content of mobile phosphorus and mobile potassium, and the concentration of total nitrogen increased significantly.

Antibacterial activity of high surface area zinc oxide nanoparticles for controlling black rot disease of Chinese kale

AbstractZinc oxide nanoparticles (ZnO NPs) are inorganic compounds listed as “generally recognized as safe” (GRAS) materials and have been used in plant production as well as for plant disease control. This study investigated the antibacterial efficacy of ZnO NPs with various surface areas against Xanthomonas campestris pv. campestris, assessed the effectiveness of ZnO NPs in controlling black rot disease in Chinese kale, and examined the influence of ZnO NPs application on soil bacterial communities. The results showed that ZnO NPs with high surface area effectively inhibited X. campestris pv. campestris by restraining growth and causing cell damage. Seed treatment and foliar spray application of high surface area ZnO NPs at 250 μg/mL significantly reduced the disease severity of black rot. Furthermore, in the greenhouse experiment, the soil bacterial communities in the treatment of plants applied with ZnO NPs did not differ from those in soil of nontreated plants. Therefore, ZnO NPs have the potential to serve as an alternative substance for plant disease management.

Biofortification of wheat in salt-affected soil through seed priming and soil application of zinc

ContextGiven the global significance of wheat production and consumption, it is imperative to achieve high yields of nutritious wheat grains. However, the inadequate availability of zinc (Zn) in salt-affected soils can aggravate salt stress, decrease wheat grain yield, and grain Zn concentration. This study compared the effectiveness of soil Zn application and seed Zn priming in increasing Zn biofortification and grain yield of Zn-biofortified wheat grown in alkaline-calcareous soil affected by salts. MethodsEighteen pots were filled with alkaline-calcareous soil containing elevated levels of soluble salts and exchangeable sodium (Na). The pots were subjected to soil Zn application (0 or 8 mg kg−1) and seed priming (control/non-, hydro-, or Zn-primed seeds) treatments, applied to a Zn-biofortified wheat (cv. Zincol-2016). Plant samples were collected at the heading and maturity stages to measure parameters related to plant growth and grain quality. FindingsSoil Zn application increased grain and straw yields across seed priming treatments by a maximum of 23 %, and seed Zn priming increased grain and straw yields across soil Zn rates by a maximum of 21 %. This yield response was accompanied by significant increases in grain potassium and Zn concentrations at maturity, as well as non-significant to significant increases in photosynthetic parameters (stomatal conductance, photosynthetic rate and transpiration rate) in flag leaves during heading. Additionally, compared to control, the combined treatment of soil Zn application and seed Zn priming decreased grain Na concentration by 14 %. Compared to control, both soil Zn application and seed Zn priming significantly increased grain Zn concentration. With the combined application treatment, the grain Zn concentration reached 27 mg kg−1, but it remained significantly below the desired level of >37 mg kg−1. Seed Zn priming decreased the phytate-to-Zn molar ratio in grains, while the treatments that received soil Zn application exhibited the lowest values of this ratio, potentially increasing Zn bioavailability to humans. ConclusionsThe findings suggest that soil Zn application is more effective in enhancing grain yield and Zn concentration, while seed Zn priming remains crucial in low-Zn and high-salt soils. Future research should optimize Zn application strategies for Zn-biofortified wheat cultivated in salt-affected fields.

Mild heating and ambient storage following gaseous chlorine dioxide treatment of chia seeds enhanced inactivation of Salmonella spp.

Chlorine dioxide (ClO2) gas is widely recognized as a potent antimicrobial agent effective against various microorganisms. Chia seeds are prone to microbial contamination, and thermal treatments are not feasible due to potential quality issues. The study is aimed at investigating the effectiveness of chlorine dioxide (ClO2) gas followed by mild heat for Salmonella reduction in chia seeds. Chia seeds were inoculated with a five-strain Salmonella cocktail and treated for 2 h at two conditions – 5 mg/L gas concentration at 80% RH and 3 mg/L at 90% RH. The samples subjected to ClO2 were mild heat treated at 40 and 60 °C for either 1 or 2 h, and Salmonella reduction is determined during one month of ambient storage. At 80% RH, ClO2 resulted in a 1.80 log reduction of Salmonella while a significantly (p < 0.05) higher log reduction of 2.82 log CFU/g was achieved at 90% RH. Subsequent mild heat treatment at 60 °C for 2 h resulted in additional log reductions of 1.14 and 1.32 for 80% and 90% RH. During the storage study, it was observed that the Salmonella population continued to decline over time. After 7 days of storage, samples treated with ClO2 at 80% and 90% RH without the application of mild heating, provided a cumulative log reduction of 2.87 and 3.81 after 7 days of storage. ClO2 treatment (3 mg/L at 90% RH) followed by mild heating (60 °C for 2 h) and 7 days of storage achieved close to 5 log reduction. The quality of chia seeds was minimally affected by ClO2 treatment, both alone and in combination with mild heating, as indicated by the insignificant changes in color values, fatty acids, and phenolic content, germination ability, and byproduct residues. This study demonstrates the effectiveness of post ClO2 mild heating and subsequent storage to enhance the microbiological safety of chia seeds.

Optimizing The Karang Mumus River School (SeSuKaMu) as An Innovation to Revitalize the River

The condition of the Karang Mumus River has been decreased from the water quality and physical condition of the river This situation has become the main focus of the government in an effort to restore the function and environment of the river as a source of meeting water needs in East Kalimantan. Community service activities aim to provide examples to the community through activities to restore the area around the river by sowing tree seeds that will be planted along the riverbanks with the Karang Mumus River Trash Pickup Movement Community which initiated the movement to save the river from ecological crisis. The method of approach is observation by discussing and interviewing information from environmental activists about SeSuKaMu, then analyzed descriptively. Community service activities such as the SeSuKaMu reforestation restoration carried out by the Community Service Team of the FISIP UNMUL Bachelor of Public Administration Study Program together with community resulted in outcomes in the form of the formation of environmental awareness and increasing community education on the importance of river conservation through activities to reforest the riverbank area and sowing tree seeds.

Effect of Cold Plasma Seed Treatment on Physiological and Biochemical Changes in Aged Mustard Seed Variety-RH0749

The low rate of seed germination is a major problem in modern agriculture. Cold-activated air plasma has been tested as a pre-treatment method to improve the rate and uniformity of the germination. In view of the widespread cultivation of mustard in India and other parts of the world, This study was conducted to determine alternative non-destructive seed treatment method based on cold plasma chemistry would offer a more viable alternative over traditional seed coating technologies Seed physiological characteristics as was modified in aged low vigour mustard seeds by coating the surface of the seeds with cold plasma process using a rotating plasma reactor during 2023-24 at CCS HAU, College of Agriculture Bawal. The source of atmospheric oxygen gas, nitrogen and heliun gas entering the plasma chamber during the reaction process determined the type of coating. Among the different treatments imposed, seed treatment with 300V at 10 Min significantly enhanced seed quality attributes viz., germination ( 92 %), root length ( 4.37 cm), shoot length ( 6.98 cm), mean seedling length (11.0 cm ), Seedling dry weight (2.67 mg) and SVI-I (1115), SVI-II (254), TDH (0.78 A480 nm) with lower electrical conductivity (14.20 dsm-1). The inhibitory effect noticed with a higher dose of plasma of 400v at 10 min suggested the need for judicious usage of these plasma frequencies in such applications. The first-ever report on the effect of new plasma state on seed germination and establishment of mustard revealed the positive influence that could be used as a seed treatment to enhance seed yield and quality.

Seedlings growth from different age of rubber trees (Hevea brasiliensis Muell. Arg.) experiencing repeated leaf fall

The budding technique propagation requires rootstock, which are raised from rubber seeds. Due to repeated leaf fall disease since 2015, seed production dropped and threatened the rubber-replanting program. Amid this situation, young trees may be a temporary source of the seeds for rootstocks, which under normal conditions is not recommended. To test this hypothesis, a comparison of germination rate, seedling growth, and biomass accumulation of the seeds from young and mature PB 260 rubber trees was carried out at Sungei Putih Research Unit, Indonesian Rubber Research Institute, North Sumatra, Indonesia. The study showed that the germination rate of the seeds was low i.e. 7.23 + 2.96 % of young plants and 21.96 + 13.51 % of mature plants respectively, both were below normal values around 70 – 80%. After germination, no significant difference was found in the growth of seedlings from young trees and their mature counterparts. The root/shoot ratio exhibited no significant difference between the two types of seedlings observed, indicating a similar biomass accumulation capability. In the condition when seed supply was limited due to leaf fall disease, the seeds of young trees might be used as a temporary source with standard selection protocols should be implied.

Superabsorbent Seed Coating and Its Impact on Fungicide Efficacy in a Combined Treatment of Barley Seeds

The technology of seed coating with superabsorbent polymer (SAP) has the potential to mitigate the negative impact of drought on seed germination and crop establishment. However, their application on the seed surface can affect the effectiveness of pesticides used for seed treatment in the protection against phytopathogens. In our work, the influence of the Aquaholder®Seed polymer coating on the effectiveness of fungicides in the protection of germinating seeds of spring barley cv. Bojos and Laudis against the fungal pathogen Bipolaris sorokiniana was studied. One-half of the seeds were first treated with fungicides, and then a polymer was applied. Fungicide efficacy was evaluated in a Petri dish test and pot test under the pathogen attack. Seed coating with SAP did not negatively affect fungicide efficacy. The percentage of germinated seeds, seedling emergence, plant height, and symptoms of the disease in the fungicide-treated variants were not significantly changed by the SAP application. Moreover, in cv. Laudis, the application of SAP alone partially protected germinating seeds against pathogen attack. The amount of pathogen DNA in plant tissues of cv. Laudis was not significantly different among seed treatments, while in cv. Bojos, the pathogen DNA increased in seeds coated with SAP alone but decreased in combined treatment with fungicides. These results demonstrated that SAP seed coating does not negatively affect the efficacy of fungicides used for seed protection against fungal pathogens.

IMPACT OF SOIL STERILIZATION FROM DIFFERENT LOCATIONS, WHEAT SEEDS CLEANING AND THEIR INTERACTIONS ON WEED CONTROL, AND YIELD AND YIELD COMPONENTS

IMPACT OF SOIL STERILIZATION FROM DIFFERENT LOCATIONS, WHEAT SEEDS CLEANING AND THEIR INTERACTIONS ON WEED CONTROL, AND YIELD AND YIELD COMPONENTS

INCREASING SOME FLAVONOIDS COMPOUNDS FOR ECHINACEA PURPUREA L. USING COPPER OXIDE NANOPARTICLES IN VITRO.

This study was aimed to increase some flavonoids active compounds production in Echinacea purpurea seedlings through plant seeds treatment with different concentrations of copper oxide nanoparticles that daignosed and characterized using AFM technique. This research was implemented at plant tissue culture labrotary of College of biotechnology - Al Nahrain University, during the period of 2021 and 2023. The experiment designed factorial within CRD using three factors and ten replicates (3X3). Sodium hypochlorite concentration (S1, S2, S3, S4) (0.0, 1, 2, 3%) represented the first factor, treatment duartion time (T1, T2, T3) (5, 10, 15min) represented the second factor and copper oxide nanoparticles concentrations (C1, C2, C3, C4) (0.0, 25, 50, 75mg/ml) represented the third factor. Results showed that the full reduction in the contamination rate of the selected E. purpurea explant recorded in 3% sodium hypochlorite at 10 and 15min. The results also showed that there were a significant increase in the shoots numbers in 50mg/ml CuNPs that recording the highest shoots numbers, the shoot length increased significantly within the 25mg/ml recording 13.5cm then decreased in 50 and 75 mg/ml and the seedlings dry weight increased significantly up to 50mg/ml CuNPs that recording the highest seddlings dry weight, then the seedlings dry weight also decreased significantly in 75mg/ml CuNPs. Also, all the analyzed flavonoids compounds using HPLC device as Echinolone, Humulene, Coumarin, Myricetin, Heperidin and Naringin concentrations were significantly increased in 50 and 75 mg/L-1 Cu ONPs, except the Humulene and Coumarin compound that significantly decreased in 75 mg/L-1 Cu ONPs in comparsion to the control.