PURPOSE: To examine the racial and ethnic differences among various types of sedentary behavior (SB) in a sample of sedentary office workers. METHODS: Study participants were recruited from five worksites in a large metropolitan city in the United States (N = 149, 67% minority, 83% female) All participants sat for at least 5-hours during a typical workday. SB was measured using a 7-item modified version of the Sedentary Behavior Questionnaire (SBQ) and the two-item sedentary behavior questions assessed in the International Physical Activity Questionnaire. Racial differences were examined with descriptive statistics as regression models. Statistical significance was determined at p < 0.05 with a two-sided test. RESULTS: Sedentary behavior ranged from a median of 0 minutes/day for viewing movies or videos to 60 min/ day for watching television and riding in or driving a vehicle. Total median time spent sitting during the weekday was 480 min/day, whereas total time spent sitting during the weekend was 270 min/day. Our adjusted models indicated statistically significant racial and ethnic differences for sitting while talking on the phone (p < 0.001), watching television (p = 0.042), riding in or driving a vehicle (p = 0.027), total sitting time during the weekdays (p = 0.008), and weekend (p = 0.012). Median scores were higher among Blacks than Whites for both sitting and talking on the phone and while watching television (Blacks = 73 and 131, Whites = 26 and 86 min/day). Total time spent sitting on the weekend was higher among Blacks than both Hispanics and Whites (Blacks = 541, Whites = 346, Hispanics = 306 min/day). However, both Blacks and Whites had higher median minutes spent in total sedentary time during the weekday than Hispanics. CONCLUSION: According to these data, racial and ethnic differences exist between various types of SB. Healthcare professionals should consider these differences when designing culturally tailored interventions designed to curve sedentary behavior. Supported by NIH Grants K01CA158000, R03NR010291, and CA016672