The viscosity of fluid plays a major role in the flow dynamics of microchannels. Viscous drag and shear forces are the primary tractions for microfluidic fluid flow. Capillary blood vessels with a few microns diameter are impacted by the rheology of blood flowing through their conduits. Hence, regenerated capillaries should be able to withstand such impacts. Consequently, there is a need to understand the flow physics of culture media through the lumen of the substrate as it is one of the vital promoting factors for vasculogenesis under optimal shear conditions at the endothelial lining of the regenerated vessel. Simultaneously, considering the diffusive role of capillaries for ion exchange with the surrounding tissue, capillaries have been found to reorient themselves in serpentine form for modulating the flow conditions while developing sustainable shear stress. In the current study, S-shaped (S1) and delta-shaped (S2) serpentine models of capillaries were considered to evaluate the shear stress distribution and the oscillatory shear index (OSI) and relative residual time (RRT) of the derivatives throughout the channel (due to the phenomena of near-wall stress fluctuation), along with the influence of culture media rheology on wall stress parameters. The non-Newtonian power-law formulation was implemented for defining rheological viscosity of the culture media. The flow actuation of the media was considered to be sinusoidal and physiological, realizing the pulsatile blood flow behavior in the circulatory network. A distinct difference in shear stress distributions was observed in both the serpentine models. The S1 model showed higher change in shear stress in comparison to the S2 model. Furthermore, the non-Newtonian viscosity formulation was found to produce more sustainable shear stress near the serpentine walls compared to the Newtonian formulation fluid, emphasizing the influence of rheology on stress generation. Further, cell viability improved in the bending regions of serpentine channels compared to the long run section of the same channel.
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