Sectioning Technique Research Articles

Dental sectioning for intraoral equine cheek teeth extractions: 29 cases.

The objectives of this retrospective study were to describe cheek teeth extraction by the sectioning technique, the decision making to use this technique and its potentially associated complications. Sectioning for dental extraction purpose was used in 29/461 (6.3%) of cases. Oro-sinusal fistula was the main post-operative complication, with 4/29 (13.7%) cases developing a macroscopic communication between the alveolus of the tooth extracted and the adjacent sinus compartment. All teeth where sectioning was attempted were successfully extracted. Sectioning for dental extraction appears to be a safe technique that can be used instead of or in addition too other minimal invasive cheek teeth extraction techniques. Thorough preoperative planning including oroscopic examination and medical imaging modalities are required to help in decision making, as well as excellent sedation and analgesia and horse compliance.

Investigation of the Experimental Shear Resistance of RC T-beams after Strengthening with Carbon Fiber-Reinforced Polymer (CFRP) Bars

This study investigates the stiffness and shear resistance of T-reinforced concrete beams that have been strengthened against shear using the embedded through-section approach. The beams were exposed to a monotonic one-point load until failure. The experimental methodology included the investigation of 12 T beams made of reinforced concrete, consisting of 2 reference beams that were not subjected to any strengthening measures and 10 reinforced beams. The 12 beams were classified into two primary categories: those with stirrups and those without stirrups. The primary factors from each group encompassed the spacing and angle of inclination pertaining to the Carbon Fiber-Reinforced Polymer (CFRP) bars placed along the central axis of the section. Various configurations were used, including varied spacing intervals and degrees of inclination. The results showed that the ratio of the CFRP shear resistance to the total section (Vf / Vsec) ranged between 10 to 21% in group one (with stirrups). This means that the Embedded Through Section (ETS) technique with CFRP bars is useful in increasing the shear resistance of reinforced concrete beams. For group two (without stirrups), this ratio ranged between 56 to 58.5%. That is, ETS with CFRP bars significantly increases the shear resistance of reinforced concrete beams without stirrups.

Caesarean section under spinal anesthesia for a mother with dilated cardiomyopathy in a resource-limited setting: a case report

Introduction and importance: Peripartum cardiomyopathy is an uncommon form of cardiomyopathy that develops in the latter stages of pregnancy or in the first few weeks following delivery. Anaesthetic management of caesarean section of a parturient with dilated cardiomyopathy is challenging due to its nature of impaired ventricular contractility coupled with cardiovascular changes during pregnancy. This is significantly worse in a resource-constrained environment with restricted access to medications and equipment. Case presentation: We report a case of a 31-year-old multigravida parturient presented with a diagnosis of dilated cardiomyopathy and a low ejection fraction undergoing emergency caesarean section. Successful management of a parturient with dilated cardiomyopathy undergoing a caesarean section under spinal anaesthesia is reported in this case report. Clinical discussion: Spinal anaesthesia is another alternative for the successful management of patients with dilated cardiomyopathy (DCM) using a low dose of bupivacaine with intrathecal adjuvants. Our report indicates that caesarean section also may be managed safely with spinal anaesthesia alone in patients with DCM, by using low-dose bupivacaine and intrathecal morphine. The slight haemodynamic changes were adjusted with fluid infusion. The use of vasopressors was not required to manage the hypotension. Conclusion: General anaesthesia, epidural alone, and epidural combined with spinal anaesthesia are the widely used anaesthetics techniques for caesarean section in patients with heart disease. Spinal anaesthesia with low-dose bupivacaine with opioids is another suitable choice. The case report is written according to the CARE guideline (https://www.care-statement.org).

Studi Kelayakan Kompetensi Profesional Guru Di Madrasah Ibtidaiyah Muhammadiyah 2 Riau

Teacher competency is very important, so a teacher must have the ability to professionally improve the implementation of their duties in the field of education. This is useful for educating the nation's children. This feasibility study aims to formulate policies related to improving the quality of education which is focused on the conditions of adequate madrasah ibtidaiyah (MI) teachers based on the professional competence possessed by each teacher. This research uses qualitative methods with cross sectional techniques to analyze data for each MI teacher based on academic qualifications according to the field of study they teach. The findings of this research show that there are still many MI teachers who do not have undergraduate academic qualifications as stipulated in Law No. 14 of 2005 concerning Teachers and Lecturers. The ratio between the number of elementary schools (SD) and MI (both private and public) in Tampan District is 5:1; or the number of elementary schools is 41 consisting of 31 state elementary schools and 10 private elementary schools, while the number of new MIs is 8, all of them have private status. It can be argued that public interest and the government's response to MI is still relatively low due, among other things, to the relative lack of competent teachers.

Harmonious Husband and Wife Relationship:

A harmonious marriage is associated with spousal satisfaction. However, in Indonesia, the divorce rate increases every year. Maintaining a harmonious family takes effort from the spouses and is discussed using stimulus, value and role theories, as internal factors. The external factor is dyadic coping, which is the ability of couples to cope with pressure together and is discussed using the systematic transactional model-STM. The purpose of the study was to determine the effect of dyadic coping on the achievement of harmonious relationships in husbands and wives. Method: non-experimental quantitative with cross sectional technique. Sample characteristics are heterosexual couples who have been married for at least 1 year, aged over 20 years, as many as 150 couples or 300 participants. Relationship satisfaction measurement tool, The Perceived Relationship Quality Components Inventory (PRQC), Cronbach Alpha reliability coefficient = 0.978, while Dyadic Coping is measured by Dyadic Coping Inventory (DCI), Cronbach Alpha reliability coefficient = 0.911. Data processing using Structural Equation Modeling (SEM), LISREL 8.80 programme, the measurement model shows all items are valid and the structural model shows a fit and significant model. The results showed the similarity of husbands and wives handling pressure, using positive dyadic coping. The difference between husbands and wives on the perception of harmonious relationship satisfaction is the aspect of love and intimacy. With these findings, it is expected to be used by counsellors, family psychologists and others to guide couples to be harmonious

Coloring the Teeth for Duplicate Denture Using a Sectional Mold Technique

This study describes a method of coloring the teeth to give natural appearance by using interchangeable flasks for duplicate denture by using a sectional mold technique.

Organization of Somatosensory Cortex in the South American Rodent Paca (Cuniculus paca)

Introduction: The study of non-laboratory species has been part of a broader effort to establish the basic organization of the mammalian neocortex, as these species may provide unique insights relevant to cortical organization, function, and evolution. Methods: In the present study, the organization of three somatosensory cortical areas of the medium-sized (5–11 kg body mass) Amazonian rodent, the paca (Cuniculus paca), was determined using a combination of electrophysiological microelectrode mapping and histochemical techniques (cytochrome oxidase and NADPH diaphorase) in tangential sections. Results: Electrophysiological mapping revealed a somatotopically organized primary somatosensory cortical area (S1) located in the rostral parietal cortex with a characteristic foot-medial/head-lateral contralateral body surface representation similar to that found in other species. S1 was bordered laterally by two regions housing neurons responsive to tactile stimuli, presumably the secondary somatosensory (S2) and parietal ventral (PV) cortical areas that evinced a mirror-reversal representation (relative to S1) of the contralateral body surface. The limits of the putative primary visual (V1) and primary auditory (A1) cortical areas, as well as the complete representation of the contralateral body surface in S1, were determined indirectly by the histochemical stains. Like the barrel field described in small rodents, we identified a modular arrangement located in the face representation of S1. Conclusions: The relative location, somatotopic organization, and pattern of neuropil histochemical reactivity in the three paca somatosensory cortical areas investigated are similar to those described in other mammalian species, providing additional evidence of a common plan of organization for the somatosensory cortex in the rostral parietal cortex of mammals.

The Potential Association of TFR1/SLC11A2/GPX4 with Ferroptosis in Mediating Lipid Metabolism Disorders in Atherosclerosis.

Atherosclerosis is the most common and significant form of arterial disease, characterized primarily by lipid accumulation and inflammatory cell infiltration as its main pathological basis. This study aims to investigate the molecular mechanisms and associated pathways by which iron accumulation may be involved in lipid metabolism abnormalities in atherosclerotic mice. Relying on ApoE-/- mouse body position observation, blood biochemical analysis, oxidative stress test and aortic tissue sectioning techniques, the effects of ferroptosis on lipid metabolism in atherosclerotic mice were analyzed. Use RT-PCR analysis and transcriptomics tests to understand the specific molecular mechanism. Our analysis reveals a correlation between Ferroptosis and elevated levels of TC, TG, ALT, AST, IL-1β, and TNF-α in the blood of atherosclerotic model mice. At the same time, it exacerbates the pathological changes of mouse aorta tissue. Our results suggest a potential link between ferroptosis and the dysregulation of TFR1/SLC11A2/GPX4 expression, along with the presence of oxidative stress, in the progression of AS. Transcriptomics results indicate that ferroptosis- mediated deterioration of atherosclerosis in ApoE-/- mice is potentially associated with cell phagocytosis, apoptosis involving TNF-α, and the expression of atherosclerotic and other process-related genes. Ferroptosis exacerbated the lipid metabolism disorder in atherosclerotic mice. The core mechanism of its effect is that ferroptosis activates the TFR1/SLC11A2/GPX4 signaling pathway, which leads to the up-regulation of oxidative stress in ApoE-/- mice, and ultimately aggravates the abnormal lipid metabolism in ApoE-/- mice.

Image quality evaluation for FIB-SEM images.

Focused ion beam scanning electron microscopy (FIB-SEM) tomography is a serial sectioning technique where an FIB mills off slices from the material sample that is being analysed. After every slicing, an SEM image is taken showing the newly exposed layer of the sample. By combining all slices in a stack, a 3D image of the material is generated. However, specific artefacts caused by the imaging technique distort the images, hampering the morphological analysis of the structure. Typical quality problems in microscopy imaging are noise and lack of contrast or focus. Moreover, specific artefacts are caused by the FIB milling, namely, curtaining and charging artefacts. We propose quality indices for the evaluation of the quality of FIB-SEM data sets. The indices are validated on real and experimental data of different structures andmaterials.

Experimental assessment of the flexural/shear strengthening effectiveness of new CFRP bars

The potential of a new carbon fibre reinforced polymer (CFRP) bar (CutInov) is explored for the simultaneous flexural and shear strengthening of reinforced concrete (RC) beams. This bar has a stick geometric configuration, with one part applied according to the near surface mounted (NSM) technique and the other according to the embedded through section (ETS) technique, both connected with a smooth transition zone. The NSM part aims to increase the flexural capacity of the RC element, whereas the ETS part, inclined at 45° relative to the RC beam’s axis, aims to provide shear strengthening and to avoid premature end debonding and rip-off failure modes to the NSM part. To avoid premature rupture at the transition zone of this bar, CFRP fan-type anchors are applied in the critical regions of the beam. An experimental programme with almost real-scale RC beams was executed, composed of a reference beam (Ref) and two beams strengthened with CutInov bars, one with (3CI_A) and the other without CFRP anchors (3CI). Compared to the Ref beam, the 3CI beam presented an increase of 55%, 71%, and 79% in terms of load at a deflection corresponding to serviceability limit state conditions, load at yield initiation of the flexural steel reinforcement, and maximum load, while 3CI_A have even developed better indicators, 61%, 95%, and 117%, demonstrating the efficiency of the CFRP anchors.

Advancements in intra-operative histology consultation through frozen section techniques

Advancements in intra-operative histology consultation through frozen section techniques

Real-time Histological Evaluation of Gastric Cancer Tissue by Using a Confocal Laser Endomicroscopic System.

The need for instant histological evaluation of fresh tissue, especially in cancer treatment, remains paramount. The conventional frozen section technique has inherent limitations, prompting the exploration of alternative methods. A recently developed confocal laser endomicroscopic system provides real-time imaging of the tissue without the need for glass slide preparation. Herein, we evaluated its applicability in the histologic evaluation of gastric cancer tissues. A confocal laser endomicroscopic system (CLES) with a Lissajous pattern laser scanning, was developed. Fourteen fresh gastric cancer tissues and the same number of normal gastric tissues were obtained from advanced gastric cancer patients. Fluorescein sodium was used for staining. Five pathologists interpreted 100 endomicroscopic images and decided their histologic location and the presence of cancer. Following the review of matched hematoxylin and eosin (H&E) slides, their performance was evaluated with another 100 images. CLES images mirrored gastric tissue histology. Pathologists were able to detect the histologic location of the images with 65.7% accuracy and differentiate cancer tissue from normal with 74.7% accuracy. The sensitivity and specificity of cancer detection were 71.9% and 76.1%. Following the review of matched H&E images, the accuracy of identifying the histologic location was increased to 92.8% (p<0.0001), and that of detecting cancer tissue was also increased to 90.9% (p<0.001). The sensitivity and specificity of cancer detection were enhanced to 89.1% and 93.2% (p<0.0001). High-quality histological images were immediately acquired by the CLES. The operator training enabled the accurate detection of cancer and histologic location raising its potential applicability as a real-time tissue imaging modality.

An intelligent human‐centric systems to diagnose breast cancer using machine learning and optimized feature selection techniques

AbstractAll over the world, breast cancer (BC) is the leading cause of cancer mortality among women. Computer‐aided methods can assist in early diagnosis. The proposed approach used SMOTE filter with Ch2 test techniques for class balance and feature section using eight different ML models Gaussian Naive Bayes (GNB), Decision Tree (DT), Random Forest (RF), Support Vector Machine (SVM) with Linear and Radial Basis Function (RBF), Logistic Regression (LR), K‐nearest neighbor (KNN) and eXtreme Gradient Boosting (XGBoost). A Ch2 test determines the top five features—glucose, HOMA, resistin, BMI, and insulin. Metrics such as accuracy, precision, recall, and F1‐Score are used to compare the performance of models. More than 99% accuracy was achieved by the proposed XGBoost model. Compared to the other breast cancer prediction models, the proposed model had an average accuracy improvement of 9.30%. As a result of our proposed model, breast cancer diagnosis will be more efficient based on risk factors. The proposed prediction model can also predict various breast cancer features. In addition to improving diagnostic decision‐support systems, the proposed model should be able to predict breast cancer disease accurately.

Effect of Thermomechanical Fatigue Loading on the Internal and Marginal Adaptation of Endocrowns Utilizing Different CAD/CAM Restorative Materials

To assess the impact of fatigue loading on the internal and marginal fit of CAD/CAM-fabricated endocrowns for restoring endodontically treated molars using different machinable blocks. A total of 72 mandibular first molars were prepared using a standardized method and were divided into four groups (n = 18), each restored with a different CAD/CAM material: group V = polymer-infiltrated ceramic (Vita Enamic); group K = partially stabilized tetragonal zirconia (Katana); group E = lithium disilicate ceramic (IPS e.max CAD; and group B = polyetheretherketone (BioHPP). Endocrowns were subjected to thermomechanical fatigue loading. Internal and marginal adaptation of the endocrowns were examined at 66 points using the sectioning technique and a stereomicroscope. Data were analyzed using one-way ANOVA and Tukey post hoc test (α = .05). Statistical tests showed that adhesive cementation resulted in significantly increased marginal and internal gap values in all regions in all four tested groups (P < .001). After thermomechanical fatigue loading, all regions in groups B and K showed a statistically significant difference, while no significant differences were found in the regions in group V (P > .05). In group E, the marginal (F = 71.00) and pulpal (F = 28.065) regions showed statistically significant differences (P < .001). Polymer-infiltrated ceramics showed the lowest gap even after thermomechanical fatigue loading and may therefore provide enhanced clinical survival of the restored tooth, favoring the use of this material for fabricating endocrown restorations.

Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block.

Agarose-based cell block (CB) technique can be modified to be combined with the frozen section technique for the preparation of a high-quality frozen-embedded CB (F-CB) from an effusion or fine-needle aspiration (FNA) cytology sample. This combined technique can be effectively used for the immunocharacterization of the hematolymphoid cells on F-CB. To demonstrate the applicability of performing diagnostic ICC on F-CB, we have analyzed the immunophenotype of the hematolymphoid cells in a series of eight cases of effusions and eight cases of FNA cytology specimens by using CB-ICC on sections cut from frozen-embedded CBs. The SurePathTM residue or cytologic material scraped off from the FNA cytology smear that was diagnostic for or suspicious of hematolymphoid malignancy was pelleted and pre-embedded in agarose. Half of the agarose-embedded pellet was frozen-embedded in OCT compound for the preparation of F-CB, while the other half was processed for the preparation of paraffin-embedded CB. Sections cut from the F-CB and P-CB were used for CB-ICC. Panels of ICC on the F-CBs could enable the immunocytochemical differential diagnosis of large cell hematologic malignancies that encompass anaplastic large cell lymphoma and other forms of large-cell hematolymphoid malignancies such as large B-cell lymphomas, anaplastic plasma cell myeloma, myeloid sarcoma, and T-lymphoblastic lymphoma. It also appeared that the small B-cell lymphomas in the effusions or FNAs could be differentially diagnosed with the aid of CB-ICC on the F-CB. A modified agarose-based CB technique can be combined with the frozen-embedded CB method for the preparation of F-CB that can be directly used for the immunocytochemical differential diagnosis of hematolymphoid cytology samples.

Comorbid Diseases and History of Close Contact Factors of Covid-19 Incidence in South Surabaya

Background: Comorbid diseases are one of the risk factors that can worsen the condition of COVID-19 patients. Patients with confirmed COVID-19 with comorbid diseases need special care, therefore, it is necessary to receive treatment in a hospital. The human-to-human transmission rate of SARS COV-2 is very fast and easy. Close contact with confirmed cases of COVID-19 is very possible for transmission to occur because this virus can be easily spread through droplet sparks. Cases and death rates from Covid-19 continue to grow over time. On March 7, 2021, the highest proportion of confirmed cases of covid-19 in Surabaya was in South Surabaya and East Surabaya, the resulting proportion was 0.82%. South Surabaya has a higher severity than East Surabaya (death rate from COVID-19 = 6%). Objectives: This study aims to analyze the magnitude of risk factors for comorbid diseases and close contact history the incidence of COVID-19 in South Surabaya. The benefit of this research is to assist the government in making policies to handle the surge in COVID-19 cases in South Surabaya. Methods: This study uses the type of observational analytical research with cross sectional design and sampling techniques used are participatory sampling. The number of samples is calculated using the formula Murti (1997). The sample size was 89. Statistical analysis used Prevalence Ratio (PR). Results: Respondents with negative PCR swab test result is 60,7% and positive result is 39,3%. Respondent with comorbid disease only as many as 17 respondents with the majority having comorbid hypertension. Respondent with close contact history is 71,9%. Comorbid diseases have a risk of covid-19 incidence in South Surabaya with PR = 2.06 while close contact history, PR = 2.34. Conclusions: Comorbid diseases have a risk of 2.06 times with the incidence of COVID-19 in South Surabaya. As for close contact history, it has a risk of 2.34 times with the incidence of COVID-19 in South Surabaya. The close contact history factor has a higher probability value in incidence of COVID-19 in South Surabaya. The Surabaya city government can pay special attention to people with comorbid diseases and increase tracing of close contacts with COVID-19 patients.

The Influence Of Organizational Culture Using The Ocai Approach On The Performance Of Health Personnel At Intan Medika Lamongan Hospital

The performance of health workers is a very important issue in order to maintain and improve health services in hospitals. Based on the results of the performance assessment for 2 years 2021 - 2022, it shows a 5% decrease in health workers at the Intan Medika Lamongan hospital. This study aims to analyze the effect of organizational culture on the performance of health workers at Intan Medika Lamongan Hospital through the Organizational Culture Assessment Instrument (OCAI) approach. The research method is descriptive quantitative and cross sectional approach and data collection techniques through questionnaires. The study population was all health workers using proportionate random sampling method. The number of respondents was 71 health workers with details of 87.3% or 62 women and 12.7%. While based on the profession, the most respondents were nurses with 56.3% and the smallest respondent was the nutrition profession, namely 1.4%. The results showed that the effect of Organizational Culture (variable X) on Performance Appraisal of Health Workers (variable Y) is positive with a significance value of 0.021.

A novel extraperitoneal cesarean section of supravesical approach: Operative techniques.

We have established a novel extraperitoneal cesarean section technique by supravesical approach. An advantage of this technique over the conventional paravesical approach is that the lower uterine segment is broadly exposed and that all operative procedures can be performed under direct vision. We present the details of this novel technique. The bladder and the peritoneum are exposed by removing the transversalis and extraperitoneal fasciae. Subsequently, a triangular area between the median umbilical ligament, the peritoneum, and the bladder is exposed. The median umbilical ligament is dissected at this site. Bladder dissection from the peritoneum is also initiated from this area and proceeds toward the lower uterine segment. Operative times for pelvic fascia dissection and bladder removal from the peritoneal surface are currently around 15-25 min. During the process of development of this technique, there have been no bladder injuries in 501 patients that caused urine leakage. The supravesical approach has been considered difficult due to the strong adhesion between the perivesical fascia and the peritoneum at the bladder fundus. In this paper, we show how to safely remove the bladder fundus from the peritoneum. The bladder can then be easily lowered down toward the lower uterine segment. Although this technique allows the lower uterine segment to be broadly exposed and all operative procedures can be performed under direct vision, a disadvantage is the comparatively long time currently required to perform it. This technique could be a valuable option for extraperitoneal cesarean section, but disadvantages must also be considered.

Detailed Seed Cone Morpho-Anatomy Provides New Insights into Seed Cone Origin and Evolution of Podocarpaceae; Podocarpoid and Dacrydioid Clades.

The study of reproductive morphology and trait evolution provides a vital insight to understand the evolutionary history of plants. The conifer family Podocarpaceae has a remarkable diversity of seed cones, with distinct morphology among the genera and with conifers in general. However, we lack a good understanding of the seed cone morpho-anatomy and trait evolution of Podocarpaceae. We investigated detailed seed cone morpho-anatomy using staining and sectioning techniques to clarify the anatomical, morphological diversity and evolution of functional traits. The presence of a fleshy receptaculum is a characteristic feature of both clades. However, species of Retrophyllum, Afrocarpus and some species of Nageia and Podocarpus form a fleshy sarcotesta-like seed coat, lacking a fleshy receptaculum. The ancestral state reconstructions show a shift between and sometimes within the genus. Although both clades demonstrate fleshiness as an ancestral trait, the shift in fleshy structures provides evidence for complex multiple evolutions of fleshy morphologies. These seed cone traits (e.g., fleshiness and size), along with the broad, flattened and well-adapted (leaf dimorphism) foliage in both clades, are largely congruent with efficient light harvesting and bird dispersal. These traits make these two clades well adapted to their environment, when growing in communities including tall and broad-leaved angiosperms (closed-canopy angiosperm forests), compared to other podocarps, making them more successful in achieving a wider distribution and species richness.

Awareness and knowledge of ovarian cancer symptoms and risk factors among female undergraduates in the university of Ibadan

Introduction: Ovarian cancer is the fifth most common malignancy among women in Africa, it affects women more frequently than any other cancer. There are various risk factors and symptoms of ovarian cancer, but women are generally found to lack knowledge on this hence leading to late diagnosis of ovarian cancer in most women. Methods: This study employed a cross sectional survey technique and was conducted among female undergraduate staying in the halls of residence in the university of Ibadan. The total student population was 1496. The sample size was determined using the Taro Yamane formula and the sample size was 351 students which was allocated proportionally to each hall of residence. The research instrument used for the study is a self-administered questionnaire. Using SPSS, the data were analyzed using the frequency, percentage, and chi-square test at a significance level of 0.05%. The chi-square test of independence was used to investigate the association between the age and awareness of ovarian cancer among females in University of Ibadan’s halls of residence. It was also used to investigate the association between the level of knowledge of ovarian cancer risk factors and symptoms among female staying in Queen Elizabeth II Hall and Obafemi Awolowo Hall of residence. Using SPSS, the data were analyzed using the frequency, percentage, and chi-square test at a significance level of 0.05%. Results: The results showed that 92% of female undergraduate students at the University of Ibadan are well-informed about ovarian cancer and majority of female undergraduates are quite knowledgeable about the symptoms and risks factors of ovarian cancer. The study found that that there is no significant relationship between the ages of the female undergraduate students in University of Ibadan and their awareness of Ovarian cancer and also conclude that there is a significant relationship between the hall of residence of the female undergraduate students in University of Ibadan and their awareness of Ovarian cancer. Conclusion: In conclusion, this study has revealed just like every other related study that most female undergraduate have a considerable high knowledge about the symptoms and risk factors of ovarian cancer however the University management should take the advantage of special occasions like world cancer day should do more sensitization on the symptoms and risk factor of ovarian cancer and other type of cancer particularly in halls of residence with lower level of knowledge on ovarian cancer.