Secretion Of Extracellular Polymeric Substances Research Articles

Revealing the response characteristics of periphyton biomass and community structure to sulfamethoxazole exposure in aquaculture water: The perspective of microbial network relationships

The widespread application of sulfonamide antibiotics in aquaculture has raised concerns about their adverse environmental impacts. Periphyton plays a crucial role in the aquatic ecosystem. In this study, we examined sulfamethoxazole (SMX) effects on the community structure and interactions of periphyton in simulated aquaculture water. Our findings indicated that the total biomass of periphyton decreased, while the biomass of periphytic algae and the secretion of extracellular polymeric substances (EPS) increased at 0.7 × 10−3 mg/L. Under higher SMX concentrations (5 mg/L and 10 mg/L), periphyton growth was severely inhibited, the microbial community structure of periphyton were sharply altered, characterized by the cyanobacteria growth suppression and decrease in the diversity index of community. Furthermore, elevated SMX concentrations (5 mg/L and 10 mg/L) increased the ratio of negative relationships from 45.4% to 49.4%, which suggested that high SMX concentrations promoted potential competition among microbes and disrupted the microbial food webs in periphyton. The absolute abundance of sul1 and sul2 genes in T2 and T3 groups were 2–3 orders of magnitude higher than those in control group after 30 days of SMX exposure, which elevated the risk of resistance gene enrichment and dissemination in the natural environment. The study contributes to our understanding of the detrimental effects of antibiotic pollution, which can induce changes in the structure and interaction relationship of microbial communities in aquaculture water.

Enhanced granulation of activated sludge in an airlift reactor for organic carbon removal and ammonia retention from industrial fermentation wastewater: A comparative study

Ammonia retention and recovery from high-nitrogenous wastewater are new concepts being used for nitrogen management. A microaerophilic activated sludge system was developed to convert organic nitrogen into ammonia and retain it for its recovery; however, the settleability of activated sludge remains a challenge. Therefore, this study proposed an aerobic granular sludge system as a potential solution. Two types of sequencing batch reactors—airlift and upflow reactors—were operated to investigate the feasibility of fast granule formation, the performance of organic carbon removal and ammonia retention, and the dynamics of microbial community composition. The operation fed with industrial fermentation wastewater demonstrated that the airlift reactor ensured a more rapid granule formation than the upflow reactor because of the high shear force, and it maintained a superior ammonia retention stability of approximately 85 %. Throughout the operational period, changes in hydraulic retention time (HRT), settling time, and exchange ratio altered the granular particle sizes and microbial community compositions. Rhodocyclaceae were replaced with Comamonadaceae, Methylophilaceae, Xanthomonadaceae, and Chitinophagaceae as core taxa instrumental in granulation, likely because of their extracellular polymeric substance secretion. As the granulation process progressed, a significant decrease in the relative abundances of nitrifying bacteria—Nitrospiraceae and Nitrosomonadaceae—was observed. The reduction of settling time and HRT enhanced granulation and inhibited the activity of nitrifying bacteria. The success in granulation for ammonia conversion and retention in this study accelerates the paradigm shift from ammonia removal to ammonia recovery from industrial fermentation wastewater.

Benzethonium chloride affects short chain fatty acids produced from anaerobic fermentation of waste activated sludge: Performance, biodegradation and mechanisms

Benzethonium chloride (BZC) is viewed as a promising disinfectant and widely applied in daily life. While studies related to its effect on waste activated sludge (WAS) anaerobic fermentation (AF) were seldom mentioned before. To understand how BZC affects AF of WAS, production of short chain fatty acids (SCFAs), characteristics of WAS as well as microbial community were evaluated during AF. Results manifested a dose-specific relationship of dosages between BZC and SCFAs and the optimum yield arrived at 2441.01 mg COD/L with the addition of 0.030 g/g TSS BZC. Spectral results and protein secondary structure variation indicated that BZC denatured proteins in the solid phase into smaller proteins or amino acids with unstable structures. It was also found that BZC could stimulate the extracellular polymeric substances secretion and reduce the surface tension of WAS, leading to the enhancement of solubilization. Beside, BZC promoted the hydrolysis stage (increased by 7.09 % to 0.030 g/g TSS BZC), but inhibited acetogenesis and methanogenesis stages (decreased by 6.85 % and 14.75 % to 0.030 g/g TSS BZC). The microbial community was also regulated by BZC to facilitate the enrichment of hydrolytic and acidizing microorganisms (i.e. Firmicutes). All these variations caused by BZC were conducive to the accumulation of SCFAs. The findings contributed to investigating the effect of BZC on AF of WAS and provided a new idea for the future study of AF mechanism.

Behavior and toxicity of silver nanoparticles to Chlorella vulgaris: A new perspective based on surface charges

The toxicity of silver nanoparticles (AgNPs) to algae is influenced by their surface charges. This study investigated the behavior and toxicity of two AgNPs modified by branched polyethyleneimine (BPEI-AgNPs with a positive charge) and citrate (CIT-AgNPs with a negative charge) on Chlorella vulgaris (C. vulgaris) to elucidate the surface charge-dependent toxicity of AgNPs to C. vulgaris. The results indicated that the BPEI-AgNPs exhibited a stronger inhibitory effect on the growth and photosynthetic activity of C. vulgaris, compared with the CIT-AgNPs. The reduction in the final superoxide dismutase content of 0.5 mg/L BPEI-AgNPs (48%) was higher than that of the CIT-AgNPs group (39%). The C. vulgaris exhibited less tolerance to the positively charged BPEI-AgNPs. The BPEI-AgNPs induced higher secretion of extracellular polymeric substances (EPS) and higher concentrations of lactate dehydrogenase (LDH) in C. vulgaris than CIT-AgNPs. The BPEI-AgNPs had a more significant up-regulation of gene expression for oxidative stress (sod2) than CIT-AgNPs, up to 2.57-fold. Genes encoding photosynthesis pathways were down-regulated, resulting in serious damage to photosynthetic system. Furthermore, the CIT-AgNPs inhibited the citrate cycle and oxidative phosphorylation metabolism, while BPEI-AgNPs disrupted the photophosphorylation pathways. These findings offer valuable insights into the effects of AgNPs with different surface charges on C. vulgaris.

Physiological and transcriptomic analysis reveals the toxic and protective mechanisms of marine microalga Chlorella pyrenoidosa in response to TiO2 nanoparticles and UV-B radiation

Concerns have been raised regarding the adverse effects of nanoparticles (NPs) on marine organisms, as an increasing number of NPs inevitably enter the marine environment with the development of nanotechnology. Owing to the photocatalytic properties, TiO2 NPs' toxicity may be aggravated by enhanced UV-B resulting from stratospheric ozone depletion. However, the molecular mechanisms of phytoplankton in response to TiO2 NPs under UV-B remains poorly understood. In this study, we integrated whole transcriptome analysis with physiological data to provide understanding on the toxic and protective mechanisms of marine Chlorella pyrenoidosa in response to TiO2 NPs under UV-B. The results indicated that the changes in gene expression could be related to the growth inhibition and TiO2 NP internalization in C. pyrenoidosa, and several molecular mechanisms were identified as toxicity response to TiO2 NPs and UV-B. Differential expression of genes involved in glycerophospholipids metabolism indicated that cell membrane disruption allowed TiO2 NPs to enter the algal cell under UV-B exposure, although the up-regulation of genes involved in the general secretory dependent pathway and the ATP-binding cassette transporter family drove cellular secretion of extracellular polymeric substances, acting as a barrier that prevent TiO2 NP internalization. The absence of changes in gene expression related to the antioxidant system may be responsible for the severe oxidative stress observed in algal cells following exposure to TiO2 NPs under UV-B irradiation. Moreover, differential expression of genes involved in pathways such as photosynthesis and energy metabolism were up-regulated, including the light-harvesting, photosynthetic electron transport coupled to photophosphorylation, carbon fixation, glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, and oxidative phosphorylation, indicating that more energy and metabolites were supplied to cope with the toxicity of TiO2 NPs and UV-B. The obtained results provide valuable information on the molecular mechanisms of response of marine phytoplankton exposed to TiO2 NPs and UV-B.

Response of antibiotic resistance genes expression and distribution on extracellular polymeric substances and microbial community in membrane biofilm during greywater treatment

This study evaluated how organic loading affects antibiotic resistance genes (ARGs) expression and distribution in the membrane biofilm. Organic surface loading rate of 4.65 g chemical oxygen demand (COD)/m2·d achieved the maximum biofilm thickness, concentration and linear alkylbenzene sulfonate (LAS) removal ratio of 136.9 ± 4.7 μm, 5.4 ± 0.1 g VSS/m2 and 99.4 %, respectively. Extracellular polymeric substances (EPS), EPS-attached LAS, and ARGs gradually increased in the membrane air inlet, middle and air outlet. AGRs and Intl1 were abundant in biofilm. LAS promoted EPS secretion, biofilm growth and ARGs proliferation. EPS, protein and carbohydrate were significantly correlated with most of biofilm ARGs, but not corrected with liquid-based ARGs. Microbial community structure impacted ARGs proliferation and transfer in the system. The findings indicated that EPS and microbial community play a crucial role in ARGs proliferation, spread and distribution, which lay the foundation for front-end control of ARGs during biofilm-based wastewater treatment.

Rapid static feeding combined with Fe2+ addition for improving the formation and stability of aerobic granular sludge in low-strength wastewater

Aerobic granular sludge (AGS) needs a long start-up time and always shows unstable performance when it is used to treat low-strength wastewater. In this study, a rapid static feeding combined with Fe2+ addition as a novel strategy was employed to improve the formation and stability of AGS in treating low-strength wastewater. Fe-AGS was formed within only 7 days and showed favorable pollutant removal capability and settling performance. The ammonia nitrogen (NH4+-N) and chemical oxygen demand (COD) concentration in the effluent were lower than 5 mg/L and 50 mg/L after day 23, respectively. The sludge volume index (SVI) and mixed liquid suspended solids (MLSS) was 37 mL/g and 2.15 g/L on day 50, respectively. Rapid static feeding can accelerate granules formation by promoting the growth of heterotrophic bacteria, but the granules are unstable due to filamentous bacteria overgrowth. Fe2+ addition can inhibit the growth of filamentous bacteria and promote the aggregation of functional bacteria (eg. Nitrosomonas, Nitrolancea, Paracoccus, Diaphorobacter) by enhancing the secretion of extracellular polymeric substances (EPS). This study provides a new way for AGS application in low-strength wastewater treatment.

Antibiofilm Effects of Oleuropein against Staphylococcus aureus: An In Vitro Study.

Staphylococcus aureus has posed a huge threat to human health and the economy. Oleuropein has antibacterial activities against various microorganisms but research on its effect on the S. aureus biofilm is limited. This research aimed to estimate the antibiofilm activities of oleuropein against S. aureus. The results suggest that the minimum inhibitory concentration of oleuropein against S. aureus ATCC 25923 was 3 mg/mL. The biomass of biofilms formed on the microplates and coverslips and the viability of bacteria were significantly reduced after the treatment with oleuropein. The scanning electron microscopy observation results indicated that the stacking thickness and density of the biofilm decreased when S. aureus was exposed to oleuropein. It had a bactericidal effect on biofilm bacteria and removed polysaccharides and proteins from mature biofilms. The effects of oleuropein on the biofilm could be explained by a reduction in bacterial secretion of extracellular polymeric substances and a change in bacterial surface hydrophobicity. Based on the above findings, oleuropein has the potential to be used against food pollution caused by S. aureus biofilms.

Robust rehabilitation of anammox system by granular activated carbon under long-term starvation stress: Microbiota restoration and metabolic reinforcement

This article investigates the buffering capacity and recovery-enhancing ability of granular activated carbon (GAC) in a starved (influent total nitrogen: 20 mg/L) anaerobic ammonium oxidation (anammox) reactor. The findings revealed that anammox aggregated and sustained basal metabolism with shorter performance recovery lag (6 days) and better nitrogen removal efficiency (84.9 %) due to weak electron-repulsion and abundance redox-active groups on GAC’s surface. GAC-supported enhanced extracellular polymeric substance secretion aided anammox in resisting starvation. GAC also facilitated anammox bacterial proliferation and expedited the restoration of anammox microbial community from a starved state to its initial-level. Metabolic function analyses unveiled that GAC improved the expression of genes involved in amino acid metabolism and sugar-nucleotide biosynthesis while promoted microbial cross-feeding, ultimately indicating the superior potential of GAC in stimulating more diverse metabolic networks in nutrient-depleted anammox consortia. This research sheds light on the microbial and metabolic mechanisms underlying GAC-mediated anammox system in low-substrate habitats.

Effects of micro-bubble aeration on the pollutant removal and energy-efficient process in a floc–granule sludge coexistence system

Abstract To investigate energy-saving approaches in wastewater treatment plants and decrease aeration energy consumption, this study successfully established a floc–granule coexistence system in a sequencing batch airlift reactor (SBAR) employing micro-bubble aeration. The analysis focused on granule formation and pollutant removal under various aeration intensities, and compared its performance with a traditional floc-based coarse-bubble aeration system. The results showed that granulation efficiency was positively associated with aeration intensity, which enhanced the secretion of extracellular polymeric substances (EPSs) and facilitated granule formation. The SBAR with the micro-aeration intensity of 30 mL·min−1 showed the best granulation performance (granulation efficiency 52.6%). In contrast to the floc-based system, the floc–granule coexistence system showed better treatment performance, and the best removal efficiencies of NH4+-N, TN, and TP were 100.0, 77.0, and 89.5%, respectively. The floc–granule coexistence system also enriched higher abundance of nutrients removal microbial species, such as Nitrosomonas (0.05–0.14%), Nitrospira (0.14–2.32%), Azoarcus (2.95–12.17%), Thauera (0.43–1.95%), and Paracoccus (0.76–2.89%). The energy-saving potential was evaluated, which indicated it is feasible for the micro-aeration floc–granule coexistence system to decrease the aeration consumption by 14.4% as well as improve the effluent.

Unraveling the two-sided effect of nitrate on aerobic granulation

Aerobic granular sludge (AGS) is a promising technology in wastewater treatment which has gained considerable attention. This study aims to unravel the two-sided effect of nitrate on AGS granulation. By feeding different concentrations of nitrate (30 and 66 mg/L), better granulation was achieved in experimental groups compared to control group without nitrate feeding. However, after three-months cultivation, the mean granule size of control group drastically exceeded the nitrate-feeding groups where disintegration of granules occurred. The variation in extracellular polymeric substances (EPS) was consistent with the trend of granulation, suggesting its important role in AGS granulation. An in-depth analysis indicated that this two-sided effect of nitrate was rooted in denitrification, which had been promoted by nitrate feeding. The short-term promoting effect of granulation was due to the alkalinity generated by denitrification, which was beneficial for calcium retention, thereby providing micronuclei for granule growth and promoting EPS secretion. The inhibiting effect of granulation stemmed from the suppressive concentration of c-di-GMP caused by denitrification, and it became pronounced under long-term cultivation, inducing a decline in EPS. In microbial level, nitrate significantly affected the denitrifying and EPS-producing bacteria, such as Micropruina, Thauera and Flavobacterium. The changes in these two types of bacteria were related to denitrification and EPS variation. This given further evidence for the two-sided effect of nitrate on AGS granulation. This study has highlighted the potential effects of nitrate on aerobic granulation and provided references for utilizing AGS in nitrate-containing wastewater.

Effect of Salinity on Performance and Microbial Community during Granulation Process in a Sequencing Batch Reactor

This study focused on the secretion of extracellular polymeric substances (EPSs), reactor nutrient removal performance and the microbial community under varying concentrations of NaCl (0, 10, 20, 30 and 40 g/L) during a granulation process in a sequencing batch reactor (SBR). The microorganisms tended to secrete higher levels of protein (PN) and polysaccharide (PS) as a protective mechanism under saline conditions, with tightly bound EPS (TB-EPS) playing a crucial role in stabilizing granules. An overall high removal rate of chemical oxygen demand (COD) throughout operation was observed. However, the removal rate of total nitrogen (TN) progressively decreased with the stepwise increase in salinity from 85.59% at 10 g/L to 64.18% at 40 g/L. The low total phosphorus (TP) removal efficiency during the operation process is due to the loss of sludge biomass and inhibition of phosphorus-accumulating bacteria activity. Moreover, salinity caused the changes in microbial community structure. Paracoccus, Thauera and unclassified_f_Rhodobacteraceae were dominant genera at 10 g, 20 g/L and 30 g/L salinity, respectively, while Azoarcus, Halomonas, unclassified_f_Flavobacteriaceaeand Vibrio replaced them at 40 g/L salinity.

Comprehensive study of the combined effects of biochar and iron-based conductive materials on alleviating long chain fatty acids inhibition in anaerobic digestion

This study investigated the feasibility of alleviating the negative influence of long-chain fatty acids (LCFAs) on anaerobic digestion by biochar, micron zero-valent iron, micron-magnetite (mFe3O4) and their combination. The results demonstrate that co-addition of biochar and 6 g/L mFe3O4 (BC+6 g/L mFe3O4) increased cumulative methane production by 50% as suffered from LCFAs inhibition exerted by 2 g/L glycerol trioleate. The BC+6 g/L mFe3O4 did best in accelerating total organic carbon degradation and volatile fatty acids conversion, through successively enriching Bacteroides, Corynebacterium, and DMER64 to dominant the bacterial community. The proportion of acetotrophic Methanothrix that could alternatively reduce CO2 to methane by accepting electrons via direct interspecies electron transfer (DIET) was 0.09% with BC+6 g/L mFe3O4, nine times more than the proportion in control. Prediction of functional genes revealed the enrichment of the bacterial secretion system, indicating that BC+6 g/L mFe3O4 promoted DIET by stimulating the secretion of extracellular polymeric substances. This study provided novel insights into combining biochar and iron-based conductive materials to enhance AD performance under LCFAs inhibition.

The influence of different nitrate concentrations on aerobic sludge granulation and the role of extracellular polymeric substances

This study investigated the influence of nitrate on aerobic granular sludge (AGS) granulation. The introduction of nitrate at 5, 15 and 20 mg L−1 promoted AGS granulation, and the promoting effect was positively correlated with nitrate concentrations. Meanwhile, exogenous nitrate significantly increased denitrification rate in the AGS system. However, granular disintegration appeared at a long-term addition of nitrate. An in-deep analysis showed that nitrate stimulated the secretion of extracellular polymeric substances (EPS), especially the content of proteins, which might be the main reason for the AGS granulation. However, the rapid and excessive increase in EPS might cause granular disintegration, as excessive EPS blocked the transmission of substrates, leading to the increase of dead cells in the granules. Besides, nitrate also altered the hydrophobicity of EPS and the content of α-helix, 3-turned helix and polymeric chain that favored aggregation, which also affected AGS granulation. From the microbial community level, nitrate induced the enrichment of denitrifying bacteria, including those that also functioned as EPS producers, such as Micropruina and Flavobacterium, resulting in the rapid increase of functional enzymes associated with amino acid synthesis, thereby promoting the secretion of proteins in EPS. Conversely, disintegration caused by mass transfer blockage might lead to the loss of EPS producing bacteria and subsequent decrease in EPS content, further accelerating granular disintegration.

Enhanced membrane fouling by microplastics during nanofiltration of secondary effluent considering secretion, interaction and deposition of extracellular polymeric substances

Microplastic (MP) has been found to influence membrane fouling during microfiltration/ultrafiltration processes in direct and indirect ways by acting as fouling components and changing microbial activities, respectively. However, there is no relevant research about the contribution of MPs to nanofiltration membrane fouling. In this study, for the first time, the impacts of MPs on membrane fouling during the nanofiltration of secondary effluent (SE) were systematically investigated from the perspective of bacterial extracellular polymeric substances (EPS) secretion, their interaction with coexisting pollutants and also deposition. Membrane flux behaviors indicate that MPs simultaneously aggravated the short-term and long-term membrane fouling resistance of nanofiltration by 46 % and 27 %, respectively. ATR-FTIR, XPS and spectrophotometry spectra demonstrate that the deteriorated membrane fouling by MPs directly resulted from the increased accumulation of protein-like, polysaccharides-like and humic-like substances on membranes. EEM spectra further confirmed that MPs preferred to induce serious cake layers, which dominated membrane flux decline but hindered pore fouling. According to CLSM and SEM-EDS mappings, MPs in SE could stimulate microbial activities and then aggravate EPS secretion, after which their interaction with Ca2+ was also enhanced in bulk solution. The cross-linker nets could promote the deposition of other unlinked pollutants on membranes. Besides, MPs could weaken the rejection of certain dissolved organic matters (from 57 % to 52 % on the 50th day of filtration) by aggravating cake-enhanced concentration polarization (CECP), but improved the average removal of inorganic salts from 58 % to 63 % by improving their back diffusion through cake layers. Based on these analyses, the mechanisms of MP-enhanced membrane fouling during the nanofiltration of SE can be thoroughly revealed.

Simultaneous methanogenesis and denitrification in an anaerobic moving bed biofilm reactor for landfill leachate treatment: Ameliorative effect of rhamnolipids

In this study, an anaerobic moving bed biofilm reactor (AnMBBR) was developed for simultaneous methanogenesis and denitrification (SMD) to treat high-strength landfill leachate for the first time. A novel strategy using biosurfactant to ameliorate the inhibition of landfill leachate on the SMD performance was proposed and the underlying mechanisms were explored comprehensively. With the help of rhamnolipids, the chemical oxygen demand (COD) removal efficiency of landfill leachate was improved from 86.0% ± 2.9% to 97.5% ± 1.6%, while methane yields increased from 50.1 mL/g-COD to 69.6 mL/g-COD, and the removal efficiency of NO3−-N was also slightly increased from 92.5% ± 1.9% to 95.6% ± 1.0%. The addition of rhamnolipids increased the number of live cells and enhanced the secretion of extracellular polymeric substances (EPS) and key enzyme activity, indicating that the inhibitory effect was significantly ameliorated. Methanogenic and denitrifying bacteria were enhanced by 1.6 and 1.1 times, respectively. Analysis of the microbial metabolic pathways demonstrated that landfill leachate inhibited the expression of genes involved in methanogenesis and denitrification, and that their relative abundance could be upregulated with the assistance of rhamnolipids addition. Moreover, extended Deraguin - Landau - Verwery - Oxerbeek (XDLVO) theory analysis indicated that rhamnolipids reduced the repulsive interaction between biofilms and pollutants with a 57.0% decrease in the energy barrier, and thus accelerated the adsorption and uptake of pollutants onto biofilm biomass. This finding provides a low-carbon biological treatment protocol for landfill leachate and a reliable and effective strategy for its sustainable application.

Enhancing microbial salt tolerance through low-voltage stimulation for improved p-chloronitrobenzene (p-CNB) removal in high-salinity wastewater

As an important raw material for the synthesis of chemical and pharmaceutical, hazardous carcinogen p-chloronitrobenzene (p-CNB) has been widely found in high-salinity wastewater which need to be treated carefully. Due to the high-salinity shock on microorganisms, conventional microbial treatment technologies usually show poor effluent quality. This study initially investigated the p-CNB removal performance of microorganisms stimulated by 1.2 V low-voltage in high-salinity wastewater under facultative anaerobic conditions and further revealed the enhanced mechanisms. The results showed that the p-CNB removal kinetic parameter kp-CNB in the electrostimulating microorganism reactor (EMR) increased by 104.37 % to 155.30 % compared to the microorganism reactor (MR) as the control group under the varying salinities (0–45 g/L NaCl). The secretion of extracellular polymeric substances (EPS) in halotolerant microorganisms mainly enhanced by 1.2 V voltage stimulation ranging from 0 g/L NaCl to 30 g/L NaCl. Protein concentration ratio of EMR to MR in loosely bound EPS achieved maximum value of 1.77 at the salinity of 15 g/L NaCl, and the same ratio in tightly bound EPS also peaked at 1.39 under the salinity of 30 g/L NaCl. At the salinity of 45 g/L NaCl, 1.2 V voltage stimulation mainly enhanced salt-in strategy of halotolerant microorganisms, and the intracellular Na+ and K+ concentration ratio of EMR to MR reached maximum and minimum values of 0.65 and 1.92, respectively. Furthermore, the results of microbial metagenomic and metatranscriptomic analysis showed the halotolerant microorganisms Pseudomonas_A and Nitratireductor with p-CNB removal ability were enriched significantly under 1.2 V voltage stimulation. And the gene expression of p-CNB removal, salt-in strategy and betaine transporter were enhanced under voltage stimulation at varying salinities. Our investigation provided a new solution which combined with 1.2 V voltage stimulation and halotolerant microorganisms for the treatment of high-salinity wastewater.

Unveiling the Biochar-Respiratory Growth of Methanosarcina acetivorans Involving Extracellular Polymeric Substances.

Biochar can be applied to diverse natural and engineered anaerobic systems. Biochar plays biogeochemical roles during its production, storage, and environmental dynamics, one of which is related to the global methane flux governed by methanotrophs and methanogens. Our understanding of relevant mechanisms is currently limited to the roles of biochar in methanotrophic growth, but less is known about the roles of biochar in methanogenic growth. Here, we demonstrated that biochar enhanced the methanogenic growth of a model methanogen, Methanosarcina acetivorans, and the role of biochar as an electron acceptor during methanogenic growth was confirmed, which is referred to as biochar-respiratory growth. The biochar-respiratory growth of M. acetivorans promoted the secretion of extracellular polymeric substances (EPS) with augmented electron transfer capabilities, and the removal of EPS significantly attenuated extracellular electron transfer. Identification and quantification of prosthetic cofactors for EPS suggest an important role of flavin and F420 in extracellular electron transfer. Transcriptomic analysis provided additional insights into the biochar-respiratory growth of M. acetivorans, showing that there was a positive response in transcriptional regulation to the favorable growth environment provided by biochar, which stimulated global methanogenesis. Our results shed more light on the in situ roles of biochar in the ecophysiology of methanogens in diverse anaerobic environments.

Phosphorus conditions change the cellular responses of Microcystis aeruginosa to perfluorooctanoic acid

Perfluorooctanoic acid (PFOA), a widespread and emerging organic contaminant of aquatic environments, has high bioaccumulation potential and high toxicity. Consequently, major concerns have been raised worldwide regarding the management of this pollutant in aquatic ecosystems. To thoroughly understand PFOA's toxic effects on aquatic organisms, systematic investigations were conducted on the cellular responses of Microcystis aeruginosa to the environmental concentrations of PFOA under various concentrations as well as phosphorus (P) conditions (concentrations and forms). The results showed that P conditions remarkably affected cyanobacterial growth as well as photosynthetic pigment content, triggered oxidative stress to disrupt the function and structure of the cell membrane, and caused changes in the extracellular and intracellular contents of microcystin-LR (MC-LR). Furthermore, PFOA (100 μg/L) was absorbed by cyanobacterial cells through the stimulation of the secretion of extracellular polymeric substances (EPS) by M. aeruginosa. After entering the cyanobacterial cells, PFOA inhibited photosynthesis, reduced P absorption, induced oxidative damage, lead to a loss of cell integrity evident in scanning electron microscope images, and increased mcyA gene expression to promote MC-LR production. Moreover, the limited P concentration and forms conditions led to increased PFOA absorption by cyanobacterial cells, which further upregulated mcyA gene expression and increased the risk of MC-LR diffusion into the aquatic environment. Our present study provided a theoretical basis and new ideas for understanding and addressing safety issues related to the presence of PFOA in aquatic environments with varying nutritional statuses.

Ecohydrological influences of biocrusts and their pathways in a desert steppe ecosystem

AbstractBiological soil crusts (biocrusts) are a prevalent form of organic cover in drylands across the world, and they play a crucial role in shaping the ecohydrological processes and functioning of dryland ecosystems. However, in desert steppe ecosystems, the ecohydrological influences of biocrusts and their pathways have not yet been thoroughly understood. Here, we examined the influences of three types of biocrusts (cyanobacterial, cyanobacterial‐moss mixed, and moss biocrusts) on soil water retention and infiltration in a typical desert steppe of northwestern China, and we also analysed the possible pathways through which biocrusts affect them. Accordingly, the field capacity (θf, indicating soil water‐holding capacity) and saturated hydraulic conductivity (Ks, indicating soil infiltrability) of each biocrust‐covered soil and bare soil were measured both at 0–5 and 5–10 cm depths, on Arenosols (loamy sand texture) and Calcisols (silty loamy texture), respectively. Our results showed that the presence of biocrusts increased surface soil (0–5 cm) water‐holding capacity while decreased soil infiltrability, creating a remarkable impact on soil water retention and infiltration. As compared to bare soil, the θf of cyanobacterial, cyanobacterial‐moss mixed, and moss biocrusts were increased by 16.5%, 21.4%, and 21.7% on Arenosols and by 9.6%, 18.9%, and 22.8% on Calcisols, while their Ks were reduced by 43.2%, 56.2%, and 38.2% on Arenosols, and by 49.0%, 68.7%, and 36.0% on Calcisols, respectively. The development of biocrusts on sandy textured soil had a more pronounced impact on both soil water retention and infiltration than loamy textured soil. We found that biocrusts mainly changed soil water retention and infiltration by increasing the contents of clay and organic matter. More specifically, the increase in clay content led to a more significant effect compared to the increase in organic matter content on Arenosols, while the opposite influence was observed on Calcisols. Among the three types of biocrusts, the soil infiltrability of the mixed biocrusts was the lowest, perhaps due to the interaction between the accumulation of clay particles by moss and the secretion of extracellular polymeric substances by cyanobacteria. Our study highlights the remarkable ecohydrological impacts of biocrusts on desert steppe ecosystems, and the results imply that it is of great necessary to integrate biocrusts and their influences into our understanding of ecohydrological processes in global drylands.