Seal Population Research Articles

120-years of ecological monitoring data shows that the risk of overhunting is increased by environmental degradation for an isolated marine mammal population: The Baltic grey seal.

The Baltic Sea is home to a genetically isolated and morphologically distinct grey seal population. This population has been the subject of 120-years of careful documentation, from detailed records of bounty statistics to annual monitoring of health and abundance. It has also been exposed to a range of well-documented stressors, including hunting, pollution and climate change. To investigate the vulnerability of marine mammal populations to multiple stressors, data series relating to the Baltic grey seal population size, hunt and health were compiled, vital demographic rates were estimated, and a detailed population model was constructed. The Baltic grey seal population fell from approximately 90,000 to as few as 3000 individuals during the 1900s as the result of hunting and pollution. Subsequently, the population has recovered to approximately 55,000 individuals. Fertility levels for mature females have increased from 9% in the 1970s to 86% at present. The recovery of the population has led to demands for increased hunting, resulting in a sudden increase in annual quotas from a few hundred to 3550 in 2020. Simultaneously, environmental changes, such as warmer winters and reduced prey availability due to overfishing, are likely impacting fecundity and health. Future population development is projected for a range of hunting and environmental stress scenarios, illustrating how hunting, in combination with environmental degradation, can lead to population collapse. The current combined hunting quotas of all Baltic Nations caused a 10% population decline within three generations in 100% of simulations. To enable continued recovery of the population, combined annual quotas of less than 1900 are needed, although this quota should be re-evaluated annually as monitoring of population size and seal health continues. Sustainable management of long-lived slowly growing species requires an understanding of the drivers of population growth and the repercussions of management decisions over many decades. The case of the Baltic grey seal illustrates how long-term ecological time series are pivotal in establishing historical baselines in population abundance and demography to inform sustainable management.

Population genomics of harbour seal Phoca vitulina from northern British Columbia through California and comparison to the Atlantic subspecies.

The harbour seal Phoca vitulina is a ubiquitous pinniped species found throughout coastal waters of the Northern Hemisphere. Harbour seal impacts on ecosystem dynamics may be significant due to their high abundance and food web position. Two subspecies exist in North America, P. v. richardii in the Pacific Ocean and P. v. vitulina in the Atlantic. Strong natal philopatry of harbour seals can result in fine-scale genetic structure and isolation by distance. Management of harbour seals is expected to benefit from improved resolution of seal population structure and dynamics. Here, we use genotyping-by-sequencing to genotype 146 harbour seals from the eastern Pacific Ocean (i.e. British Columbia (BC), Oregon and California) and the western Atlantic Ocean (i.e. Québec, Newfoundland and Labrador). Using 12,742 identified variants, we confirm the recently identified elevated genetic diversity in the eastern Pacific relative to the western Atlantic and greatest differentiation between the subspecies. Further, we demonstrate that this is independent of reference genome bias or other potential technical artefacts. Coast-specific analyses with 8933 and 3828 variants in Pacific and Atlantic subspecies, respectively, identify divergence between BC and Oregon-California, and between Québec and Newfoundland-Labrador. Unexpected PCA outlier clusters were observed in two populations due to cryptic relatedness of individuals; subsequently, closely related samples were removed. Admixture analysis indicates an isolation-by-distance signature where Oregon seals contained some of the BC signature, whereas California did not. Additional sampling is needed in the central and north coast of BC to determine whether a discrete separation of populations exists within the region.

Diminishing numbers of male southern elephant seals (Mirounga leonina Pinnipedia: Phocidae, Linnaeus, 1758) at the Vestfold Hills, East Antarctica (1957–2022)

Abstract A proportion of the southern elephant seal (Mirounga leonina) population that breeds in the Kerguelen Plateau region seasonally migrates between their natal sub-Antarctic islands and moult haul-out locations on the Antarctic coastline. Analyses of survey data collated for one moult location at the Vestfold Hills, East Antarctica, showed that there had been no appreciable change in the timing (phenology) of maximum seal arrivals between 1974 and 2022; however, the maximum number of seals moulting at that site had declined by ~90% over the same time interval. Spatial analyses showed rates of population change were survey area dependent, as seal numbers decreased most rapidly at haul-out areas closest to the permanently occupied Davis Station, suggesting that a relationship exists between seal numbers and human activities. The range of potential factors that contribute to population change for southern elephant seals moulting at the Vestfold Hills includes changes in status at primary source populations, one of which has not been surveyed since the 1990s, and species relocation. Should numbers of southern elephant seals in the Vestfold Hills continue to decrease at the current average rate of change (-7.78 seals/year) the species could vanish from the area by c. 2040.

Robustly estimating the demographic contribution of immigration: Simulation, sensitivity analysis and seals.

Identifying important demographic drivers of population dynamics is fundamental for understanding life-history evolution and implementing effective conservation measures. Integrated population models (IPMs) coupled with transient life table response experiments (tLTREs) allow ecologists to quantify the contributions of demographic parameters to observed population change. While IPMs can estimate parameters that are not estimable using any data source alone, for example, immigration, the estimated contribution of such parameters to population change is prone to bias. Currently, it is unclear when robust conclusions can be drawn from them. We sought to understand the drivers of a rebounding southern elephant seal population on Marion Island using the IPM-tLTRE framework, applied to count and mark-recapture data on 9500 female seals over nearly 40 years. Given the uncertainty around IPM-tLTRE estimates of immigration, we also aimed to investigate the utility of simulation and sensitivity analyses as general tools for evaluating the robustness of conclusions obtained in this framework. Using a Bayesian IPM and tLTRE analysis, we quantified the contributions of survival, immigration and population structure to population growth. We assessed the sensitivity of our estimates to choice of multivariate priors on immigration and other vital rates. To do so we make a novel application of Gaussian process priors, in comparison with commonly used shrinkage priors. Using simulation, we assessed our model's ability to estimate the demographic contribution of immigration under different levels of temporal variance in immigration. The tLTRE analysis suggested that adult survival and immigration were the most important drivers of recent population growth. While the contribution of immigration was sensitive to prior choices, the estimate was consistently large. Furthermore, our simulation study validated the importance of immigration by showing that our estimate of its demographic contribution is unlikely to result as a biased overestimate. Our results highlight the connectivity between distant populations of southern elephant seals, illustrating that female dispersal can be important in regulating the abundance of local populations even when natal site fidelity is high. More generally, we demonstrate how robust ecological conclusions may be obtained about immigration from the IPM-tLTRE framework, by combining sensitivity analysis and simulation.

The significance of shooting angle in seal shooting.

The present study aimed to investigate the relationship between shooting angle to the head and animal welfare outcomes in the hunt of young harp seals (Pagophilus groenlandicus). The study population consisted of young harp seals belonging to the Greenland Sea harp seal population. A sample of 171, 2-7 weeks old, weaned harp seals of both sexes were included. The study was conducted as an open, randomised parallel group designed trial during the regular hunt. The animals were allocated into four groups, A-D, according to the observed shooting angle to the head, defined as the angle between the direction of the shot and the longitudinal axis of the animal's head: (A) directly from the front; (B) obliquely from the front; (C) directly from the side; and (D) obliquely or directly from behind. Instantaneous death rate (IDR) and time to death (TTD) were the main variables. The mean IDR differed significantly between groups and was highest in group B (96.8%) and lowest in group C (66.7%). For all groups combined it was 84.2%. The mean TTD for seals not rendered instantaneously unconscious or dead (n = 27) differed significantly between groups and was shortest in group A (16 s) and longest in group C (85 s). However, the number of animals included in the TTD analysis was limited. In conclusion, based on the significantly higher IDR, the shooting angle obliquely from the front is recommended to help achieve the best animal welfare outcomes during the hunt of young harp seals.

АНАЛИЗ ДИНАМИКИ СЕВЕРНОГО МОРСКОГО КОТИКА (CALLORHINUS URSINUS) ОСТРОВА ТЮЛЕНИЙ НА ОСНОВЕ ПРОСТЕЙШЕЙ МАТЕМАТИЧЕСКОЙ МОДЕЛИ ЛОКАЛЬНОЙ ПОПУЛЯЦИИ

The northern fur seal (Callorhin usursinus), inhabiting North Pacific, has long been a valuable commercial target. This work continues a series of studies aimed at analyzing the dynamics and reproductive characteristics of the northern fur seal population on the Tyuleniy Island. The paper provides analyzes of intrapopulation parameters influence on the population sex ratio and pups production.

АНАЛИЗ СТРУКТУРЫ ПРОМЫСЛА И ДИНАМИКИ РОСТА СТАДА СЕВЕРНОГО МОРСКОГО КОТИКА (CALLORHINUS URSINUS) ОСТРОВА ТЮЛЕНИЙ В ПЕРИОД ЭКСПЛУАТАЦИИ И ПОСЛЕ ЕЁ ЗАВЕРШЕНИЯ

Based on a long time series providing biological monitoring data of the Northern fur seal population on Tyuleny Island and detailed information on the harvesting characteristics of this population, we have analyzed trends in its growth. The research results of changes in the average reproductive success of bulls and the age structure of Northern fur seal males on Tyuleny Island are presented as indicators of harvesting impact on the reproductive potential of the population. Evidence has shown that selective harvesting was practiced, where most productive bulls were removed from the population. This led to a significant change in the age structure of the population and a significant reduction in the population growth. Despite a slight increase in the natural survival of almost all age groups, the population growth slowed down sharply. The proximity of the growth potential indicator to the critical unitary value revealed in recent years leads to a bleak forecast. Further growth in the Northern fur seal population, if any, will not be rapid. To accelerate it, it is necessary to provide a new natural restructuring of the population structure, improving its qualitative composition and increasing the proportion of males with high reproductive success.

Spring haul-out behavior of seals in the Bering and Chukchi Seas: implications for abundance estimation.

Ice-associated seals rely on sea ice for a variety of activities, including pupping, breeding, molting, and resting. In the Arctic, many of these activities occur in spring (April through June) as sea ice begins to melt and retreat northward. Rapid acceleration of climate change in Arctic ecosystems is therefore of concern as the quantity and quality of suitable habitat is forecast to decrease. Robust estimates of seal population abundance are needed to properly monitor the impacts of these changes over time. Aerial surveys of seals on ice are an efficient method for counting seals but must be paired with estimates of the proportion of seals out of the water to derive population abundance. In this paper, we use hourly percent-dry data from satellite-linked bio-loggers deployed between 2005 and 2021 to quantify the proportion of seals hauled out on ice. This information is needed to accurately estimate abundance from aerial survey counts of ice-associated seals (i.e., to correct for the proportion of animals that are in the water, and so are not counted, while surveys are conducted). In addition to providing essential data for survey 'availability' calculations, our analysis also provides insights into the seasonal timing and environmental factors affecting haul-out behavior by ice-associated seals. We specifically focused on bearded (Erignathus barbatus), ribbon (Histriophoca fasciata), and spotted seals (Phoca largha) in the Bering and Chukchi seas. Because ringed seals (Phoca (pusa) hispida) can be out of the water but hidden from view in snow lairs analysis of their 'availability' to surveys requires special consideration; therefore, they were not included in this analysis. Using generalized linear mixed pseudo-models to properly account for temporal autocorrelation, we fit models with covariates of interest (e.g., day-of-year, solar hour, age and sex class, wind speed, barometric pressure, temperature, precipitation) to examine their ability to explain variation in haul-out probability. We found evidence for strong diel and within-season patterns in haul-out behavior, as well as strong weather effects (particularly wind and temperature). In general, seals were more likely to haul out on ice in the middle of the day and when wind speed was low and temperatures were higher. Haul-out probability increased through March and April, peaking in May and early June before declining again. The timing and frequency of haul-out events also varied based on species and age-sex class. For ribbon and spotted seals, models with year effects were highly supported, indicating that the timing and magnitude of haul-out behavior varied among years. However, we did not find broad evidence that haul-out timing was linked to annual sea-ice extent. Our analysis emphasizes the importance of accounting for seasonal and temporal variation in haul-out behavior, as well as associated environmental covariates, when interpreting the number of seals counted in aerial surveys.

Baikal seal (<i>Pusa sibirica</i> Gmelin 1788, Phocidae). 1. Results of the experiment on heightened commercial withdrawal (retrospective evaluation)

Dynamics of the main parameters of the Baikal seal population (age and sex structure, size and weight indices, reproductive activity) in the years of experiment with heightened commercial withdrawal of the seals (1977–1985) is analyzed on the base of primary field data collected during the spring hunting. The problem of natural limitation for the population abundance is discussed for the case when further growth is limited by carrying capacity of the habitat. The observed changes of age, sex, size, and reproductive parameters reflected intrapopulation processes of the abundance regulation, weakly associated with trophic and density factors. Despite intensive commercial load and negative consequences of unusually early ice melting in 1981, the number of Baikal seal grew until the late 1980s. The withdrawal did not lead to expected improvement of the population status that was explained by underestimation of the stock. Low level (or complete absence) of biological substantiation for the experiment is noted.

Gene expression and trace elements in Greenlandic ringed seals (Pusa hispida)

Marine top predators such as ringed seals biomagnify environmental contaminants; and with the increasing human activities in the Arctic, ringed seals are exposed to biologically significant concentrations of trace elements resulting in reproductive impairment, immunosuppression, and neurological damages. Little is known about the molecular effects of heavy metals on these vulnerable apex predators suffering from a rapidly changing Arctic with significant loss of sea-ice. In the present study, concentrations of cadmium (Cd), mercury (Hg) and selenium (Se) were measured in liver of sixteen Greenlandic ringed seals (nine adults and seven subadults) together with molecular biomarkers involved in bio-transformation, oxidative stress, endocrine disruption and immune activity in blood and blubber. The concentrations of trace elements increased in the following order: Hg > Se > Cd with levels of mercury and selenium being highest in adults. Aryl hydrocarbon receptor nuclear translocator (ARNT), peroxisome proliferator activated receptor alpha (PPARα, estrogen receptor alpha (ESR1), thyroid hormone receptor alpha (TRα) and interleukin – 2 (IL-2) mRNA transcript levels were highest in blubber, while heat shock protein 70 (HSP70) and interleukin – 10 (IL-10) were significantly higher in blood. There were no significant correlations between the concentrations of trace elements and mRNA transcript levels suggesting that stressors other than the trace elements investigated are responsible for the changes in gene expression levels. Since Hg seems to increase in Greenlandic ringed seals, there is a need to re-enforce health monitoring of this ringed seal population.

Mutualism in marine wildlife value orientations on Cape Cod: Conflict and consensus in the sea and on the shore

Wildlife value orientations drive attitudes and behavior related to wildlife species. In the United States, a shift in value orientations toward wildlife from domination to mutualism has been well-documented. Few studies, however, have examined value orientations toward wildlife in the marine environment among multiple stakeholders. A 2021 survey of residents, commercial fishers, and tourists on Cape Cod, Massachusetts, USA measured marine wildlife value orientations in the context of intense stakeholder conflicts over rebounding populations of seals and white sharks. Respondents in all three stakeholder groups were more oriented toward mutualism than domination, but differed in intensity. Tourists scored highest on mutualism and lowest on domination, followed by residents. Commercial fishers had the highest domination and lowest mutualism scores, but their mutualism values exceeded their domination values. The Potential for Conflict Index2 revealed consensus on mutualism and domination measures was highest among tourists and lowest among commercial fishers, indicating a greater diversity of commercial fisher views. The shared mutualism orientation provides a foundation for coexistence in the face of marine environment challenges.

Hybrids Between Gray Seals (Halichoerus grypus) and Spotted Seals (Phoca largha): A Case of Xeno-Breeding Preference in Pinnipeds

Generally, a species is defined as an independent unit that is reproductively isolated from others. However, deviations from this definition are not uncommon. For example, cross-fertilization in pinnipeds has been reported among at least 10 species. Herein, we describe an unexpected hybridization that occurred between female gray seals (Halichoerus grypus) and male spotted seals (Phoca largha) in the presence of adult male gray seals in a mixed-species seal population under human care. To our knowledge, these are the first cases of gray and spotted seal hybrids ever reported. The three hybridized offspring had the appearance of a gray seal body and a spotted seal head. Microsatellite DNA markers were employed as genetic evidence to further support the hybridization events. Our study suggests a natural preference for interspecies hybridization between female gray seals and male spotted seals in human care. Following that, potential causes of cross-species hybridization, including female preference, male competition, and some other factors, are discussed.

Abundance and distribution of the Caspian seals on ice in the Russian part of the Northern Caspian in February — March 2023

The aim of this research was to assess the current abundance of the Caspian seal population on ice in the Russian part of the North Caspian using instrumental methods of aerial survey in the infrared and optical ranges using a manned aircraft and UAV.The main method of the research was a multispectral aerial survey, the essence of which is to survey seal haulouts synchronously in the infrared and optical ranges. In winter, 90% of all mature individuals gather on the ice substrate of the Northern Caspian for reproduction, lactation and mating and spend a significant part of their time outside the water, which greatly facilitates their detection and counting. The aerial survey was carried out in parallel from two types of aircraft — the La‑8 manned amphibious aircraft and unmanned aerial vehicles equipped with high-resolution cameras in optical and infrared modes, which ensure reliable registration of seals on ice.Novelty: the advantage of joint use of a manned aircraft and a UAV equipped with special photo and infrared equipment is noted.As a result of the research, the flights were carried out from February 20 to March 5, 2023 in the daytime in good visibility conditions at an altitude of 200 to 300 m. amounted to 19704 individuals, including 4062 puppies. The animals were located in the southeastern part of the ice cover near the air demarcation line between the Russian Federation and the Republic of Kazakhstan on the ice edge. An increase in the number of seals was recorded due to migrating individuals from Kazakhstan. Breeding of the Caspian seal in winter 2022/2023 proceeded in a satisfactory manner. During the flight, it was found that there were no fresh releases of seals on the coast of Dagestan. Separate old carcasses, partially torn to pieces, were registered only on the shores of the Agrakhan Peninsula.Practical value: the obtained data are needed to assess the current abundance of the Caspian seal population in the Caspian Sea.

Long-term decline in body condition of female Australian fur seals: potential causes and implications

The Australian fur seal (Arctocephalus pusillus doriferus, AUFS) population is still recovering from the over-exploitation of the commercial-sealing era (18th and 19th centuries). While the population is considered to be only < 47% of its pre-harvest size, it now represents the greatest resident marine predator biomass in the south-eastern Australian marine ecosystem. The region is experiencing rapid environmental change and, as a keystone predator species, the AUFS is an indicator of ecosystem health. In the present study, the body mass, standard length and body condition index (BCI) were analysed between 1997-2021 in adult female AUFS provisioning pups on Kanowna Island (northern Bass Strait), the third largest colony for the species. While substantial inter-annual fluctuations were observed, there was no temporal trend in standard length during the 23-year study period. In contrast, body mass and, consequently, BCI decreased significantly, suggesting the population is experiencing changing nutritional conditions. While these changes do not appear to be due to competition with commercial fisheries or population expansion, weak but significant negative relationships were observed between BCI and 1-year lagged sea surface temperature and summer zonal winds in the Bonney Upwelling region, and both current- and 2-year lagged Indian Ocean Dipole (IOD). These findings suggest the BCI of AUFS may continue to decline under predicted climate change conditions. While a lack of a concurrent decline in pup production could indicate a degree of nutritional tolerance or flexibility in energy allocation, further monitoring is required to assess decreases in reproductive parameters (e.g., birth mass, pre-weaning growth rates) or vital rates, which would be expected with continued nutritional stress.

Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals.

Studies on host-parasite systems that have experienced distributional shifts, range fragmentation, and population declines in the past can provide information regarding how parasite community richness and genetic diversity will change as a result of anthropogenic environmental changes in the future. Here, we studied how sequential postglacial colonization, shifts in habitat, and reduced host population sizes have influenced species richness and genetic diversity of Corynosoma (Acanthocephala: Polymorphidae) parasites in northern European marine, brackish, and freshwater seal populations. We collected Corynosoma population samples from Arctic, Baltic, Ladoga, and Saimaa ringed seal subspecies and Baltic gray seals, and then applied COI barcoding and triple-enzyme restriction-site associated DNA (3RAD) sequencing to delimit species, clarify their distributions and community structures, and elucidate patterns of intraspecific gene flow and genetic diversity. Our results showed that Corynosoma species diversity reflected host colonization histories and population sizes, with four species being present in the Arctic, three in the Baltic Sea, two in Lake Ladoga, and only one in Lake Saimaa. We found statistically significant population-genetic differentiation within all three Corynosoma species that occur in more than one seal (sub)species. Genetic diversity tended to be high in Corynosoma populations originating from Arctic ringed seals and low in the landlocked populations. Our results indicate that acanthocephalan communities in landlocked seal populations are impoverished with respect to both species and intraspecific genetic diversity. Interestingly, the loss of genetic diversity within Corynosoma species seems to have been less drastic than in their seal hosts, possibly due to their large local effective population sizes resulting from high infection intensities and effective intra-host population mixing. Our study highlights the utility of genomic methods in investigations of community composition and genetic diversity of understudied parasites.

Genetic diversity and demographic history of the leopard seal: A Southern Ocean top predator.

Leopard seals (Hydrurga leptonyx) are top predators that can exert substantial top-down control of their Antarctic prey species. However, population trends and genetic diversity of leopard seals remain understudied, limiting our understanding of their ecological role. We investigated the genetic diversity, effective population size and demographic history of leopard seals to provide fundamental data that contextualizes their predatory influence on Antarctic ecosystems. Ninety leopard seals were sampled from the northern Antarctic Peninsula during the austral summers of 2008-2019 and a 405bp segment of the mitochondrial control region was sequenced for each individual. We uncovered moderate levels of nucleotide (π = 0.013) and haplotype (Hd = 0.96) diversity, and the effective population size was estimated at around 24,000 individuals (NE = 24,376; 95% CI: 16,876-33,126). Consistent with findings from other ice-breeding pinnipeds, Bayesian skyline analysis also revealed evidence for population expansion during the last glacial maximum, suggesting that historical population growth may have been boosted by an increase in the abundance of sea ice. Although leopard seals can be found in warmer, sub-Antarctic locations, the species' core habitat is centered on the Antarctic, making it inherently vulnerable to the loss of sea ice habitat due to climate change. Therefore, detailed assessments of past and present leopard seal population trends are needed to inform policies for Antarctic ecosystems.

Evaluating the importance of individual heterogeneity in reproduction to Weddell seal population dynamics using integral projection models.

Identifying and accounting for unobserved individual heterogeneity in vital rates in demographic models is important for estimating population-level vital rates and identifying diverse life-history strategies, but much less is known about how this individual heterogeneity influences population dynamics. We aimed to understand how the distribution of individual heterogeneity in reproductive and survival rates influenced population dynamics using vital rates from a Weddell seal population by altering the distribution of individual heterogeneity in reproduction, which also altered the distribution of individual survival rates through the incorporation of our estimate of the correlation between the two rates and assessing resulting changes in population growth. We constructed an integral projection model (IPM) structured by age and reproductive state using estimates of vital rates for a long-lived mammal that has recently been shown to exhibit large individual heterogeneity in reproduction. Using output from the IPM, we evaluated how population dynamics changed with different underlying distributions of unobserved individual heterogeneity in reproduction. Results indicate that the changes to the underlying distribution of individual heterogeneity in reproduction cause very small changes in the population growth rate and other population metrics. The largest difference in the estimated population growth rate resulting from changes to the underlying distribution of individual heterogeneity was less than 1%. Our work highlights the differing importance of individual heterogeneity at the population level compared to the individual level. Although individual heterogeneity in reproduction may result in large differences in the lifetime fitness of individuals, changing the proportion of above- or below-average breeders in the population results in much smaller differences in annual population growth rate. For a long-lived mammal with stable and high adult-survival that gives birth to a single offspring, individual heterogeneity in reproduction has a limited effect on population dynamics. We posit that the limited effect of individual heterogeneity on population dynamics may be due to canalization of life-history traits.

Spatial Variability of Macro- and Mesozooplankton in the Bransfield Strait in January 2020

The spatial distribution of the biomass of macro- and mesozoopankton species, as well as the size and sex structure of the species Euphausiasuperba (krill), Salpathompsoni, and Ihlearacovitzai in Bransfield Strait during the Antarctic summer of 2020 were studied. Krill is at the heart of the food chain of the Antarctic ecosystem because numerous populations of seabirds, seals and whales feed on krill. In recent decades, the Antarctic ecosystem has been under significant changes caused by global warming, but the nature and extent of this impact on krill stocks, especially its juveniles in the Bransfield Strait, remains insufficiently studied. In recent decades, the Antarctic ecosystem has been experiencing significant changes associated with global warming, but the nature and extent of this impact on krill stocks, especially its juveniles in the Bransfield Strait, remains insufficiently studied. At the same time, this particular region is a highly productive zone of the Antarctic ecosystem and one of the areas where concentrations of Antarctic krill accumulate. The aim of this study was to study the spatial variability of the structure, numbers and biomass of zooplankton in the Bransfield Strait during the Antarctic summer of 2020. Zooplankton samples in Bransfield Strait were collected with Bongo net in January 2020. The samples were processed on the research vessel in the intervals between sampling according to the standard procedure. At all stations studied, the main zooplankton biomass was by salps (S. thompsoni and I. racovitzai). According to the index of coenotic significance, salps were of primary importance in the zooplanktocenosis, in which E. superba occupied the third place with biomass ranged from 6.3 to 96.3 mg/m3, and krill was meet only in the northwestern deep-water part of the Bransfield Strait. This is due to food competition between salps (S. thompsoni, I. racovitzai) and krill. The presence of krill in the deep-water part of the Bransfield Strait is explained by its introduction from the Bellingshausen Sea due to the prevalence of western winds. The biomass of other zooplankton species in all areas of the strait happened very small in comparison with that of salps and krill and was distributed uniformly. When comparing the ratio of the biomass of the above species obtained during the expedition with the data of the 90s and 2000s, there is a clear trend of an increase in salps and a decrease in E. superba, that is probably associated with the general trend of an increase in water temperature in the Antarctic waters. Thus, the example of Bransfield Strait clearly shows a negative correlation between the biomass of salps and krill.

Northeastern coast of Lake Baikal as a habitat of baikal seal <i>Pusa sibirica</i> in the summer period

Filling of the rookeries of baikal seal Pusa sibirica on the northeastern coast of Lake Baikal is surveyed instrumentally for the first time within the boundaries of protected area. The main coastal haulouts of the species are located in this area, so all places suitable by their lithological parameters for the seals rookeries were examined to assess intensity of their use in modern conditions of anthropogenic impact. In total, ≈ 246 km of the coastline were traced, including 170 km surveyed with unmanned aerial vehicle DJI (in 317 minutes) and visual observations on the rest of the coast with binoculars and ground photo- and video-recording. Only 9 % of the surveyed coastline (> 21 km) is suitable for rookeries, located mainly in the northern part of the area. Unfavorable lithological properties (sands, sandy pebbles, small stones, rocks) prevail on the other part of the coast, though many places (usually big boulders) are available for small rookeries there, too. Currently all these places are almost never used by seals, except of short, sporadic haulouts, mainly in June, immediately after complete disappearance of the floating ice. The main reason why the animals leave their traditional rookeries in this area in July-August is an intensive anthropogenic load, in particular at the places where the largest rookeries existed previously, as the bays Frolikha, Ayaya, Khakusy, capes Khaman-Kit, Pongonye, and some others. The leaving of rookeries contradicts to increasing needs of seals for a solid substrate to complete their molt in conditions of climate warming. Possibly, the threat of losing the northeastern coast as a summer habitat for a significant part of the baikal seal population is underestimated.

Occurrence and antimicrobial resistance of Salmonella species and potentially pathogenic Escherichia coli in free-living seals of Canadian Atlantic and eastern Arctic waters.

Seal populations in Canadian waters provide sustenance to coastal communities. There is potential for pathogenic and/or antimicrobial-resistant bacteria to transfer to humans through inadvertent faecal contamination of seal products. The objective of this study was to investigate the occurrence and potential antimicrobial resistance of Salmonella spp., Escherichia coli and Listeria monocytogenes in faecal samples collected from grey seals (Halichoerus grypus) in the Gulf of St. Lawrence and from ringed seals (Pusa hispida) in Frobisher Bay and Eclipse Sound, Nunavut, Canada. Grey seals were harvested during commercial hunts or during scientific sampling; ringed seals were collected by Inuit hunters during subsistence harvests. Virulence genes defining pathogenic E. coli were identified by PCR, and antimicrobial susceptibility testing was performed on recovered isolates. In grey seals, E. coli was detected in 34/44 (77%) samples, and pathogenic E. coli (extraintestinal E. coli [ExPEC], enteropathogenic E. coli [EPEC] or ExPEC/EPEC) was detected in 13/44 (29%) samples. Non-susceptibility to beta-lactams and quinolones was observed in isolates from 18 grey seals. In ringed seals from Frobisher Bay, E. coli was detected in 4/45 (9%) samples; neither virulence genes nor antimicrobial resistance was detected in these isolates. In ringed seals from Eclipse Sound, E. coli was detected in 8/50 (16%) samples and pathogenic E. coli (ExPEC and ExPEC/EPEC) in 5/50 (10%) samples. One seal from Eclipse Sound had an E. coli isolate resistant to beta-lactams. A monophasic Salmonella Typhimurium was recovered from 8/50 (16%) seals from Eclipse Sound. All Salmonella isolates were resistant to ampicillin, streptomycin, sulfisoxazole and tetracycline. L. monocytogenes was not detected in any sample. These findings suggest that seals may act as important sentinel species and as reservoirs or vectors for antimicrobial-resistant and virulent E. coli and Salmonella species. Further characterization of these isolates would provide additional insights into the source and spread of antimicrobial resistance and virulence genes in these populations of free-living seals.