Seabirds Research Articles

The genus Contracaecum Raillet & Henry (Nematoda: Anisakidae): host and geographical distribution on Neotropical and Antarctic species

Nematodes of the genus Contracaecum Raillet & Henry, 1912 (Anisakidae, Contracaecinae) have a worldwide distribution. The taxonomy of the genus Contracaecum is well-known nowadays due to several morphometric studies, scanning electron microscopy, and molecular biology. The aim of this work was to review, clarify, and summarize the valid species of the genus Contracaecum parasitizing piscivorous sea birds and mammals from both the Neotropical and Antarctic regions reviewing all scientific available papers and electronic searching data up to date. A checklist on Neotropical and Antarctic Contracaecum spp. was organized through a revision of scientific papers and original descriptions. The systematic online search and the most updated papers were obtained through SCOPUS, Google Scholar, PubMed, Medline, World Register of Marine Species, etc. We provide information about hosts, Neotropical and Antarctic localities where worms were collected, references, molecular markers, and Genbank accession numbers. Twenty-five Neotropical and Antarctic Contracaecum species have been recorded up to date and checked out as valid ones according to the most updated data. Twenty-one species parasitize exclusively fish-eating birds, two species were reported only on marine mammals, and the other two parasitize both sea birds and mammals. A total of 20 Contracaecum species are exclusively reported for the Neotropical region, three only for Antarctic hosts, and two species were reported parasitizing both Neotropical and Antarctic hosts. Several Contracaecum species (10) have been corroborated by molecular analysis of different genetic markers. After reviewing all morphological descriptions of the Contracaecum species, and despite most of them have been characterized only by morphometric methods, we are convinced that all species listed in this work correspond to good and valid Contracaecum Neotropical and Antarctic species. Present results indicate that more taxonomic and molecular studies are needed to advance the understanding of the distribution and host specificity of the Contracaecum species.

Wind-driven upwelling of iron sustains dense blooms and food webs in the eastern Weddell Gyre

The Southern Ocean is a major sink of anthropogenic CO2 and an important foraging area for top trophic level consumers. However, iron limitation sets an upper limit to primary productivity. Here we report on a considerably dense late summer phytoplankton bloom spanning 9000 km2 in the open ocean of the eastern Weddell Gyre. Over its 2.5 months duration, the bloom accumulated up to 20 g C m−2 of organic matter, which is unusually high for Southern Ocean open waters. We show that, over 1997–2019, this open ocean bloom was likely driven by anomalies in easterly winds that push sea ice southwards and favor the upwelling of Warm Deep Water enriched in hydrothermal iron and, possibly, other iron sources. This recurring open ocean bloom likely facilitates enhanced carbon export and sustains high standing stocks of Antarctic krill, supporting feeding hot spots for marine birds and baleen whales.

A framework for improving treatment of uncertainty in offshore wind assessments for protected marine birds

AbstractGovernments worldwide are setting ambitious targets for offshore renewable energy development (ORD). However, deployment is constrained by a lack of understanding of the environmental consequences of ORD, with impacts on protected birds forming a key environmental consenting challenge. Assessing the impacts of ORD on marine birds is challenging, utilizing interlinked approaches to understand complex behavioural, energetic, and demographic processes. Consequently, there is considerable uncertainty associated with ORD assessments for marine birds, with current methods failing to quantify uncertainty in a scientifically robust, evidence-based manner. This leads to a high degree of precaution and a lack of confidence in the evidence used to inform ORD consenting decisions. We review the methods used to estimate ornithological ORD impacts in the UK, a country at the forefront of ORD. We identify areas in which uncertainty quantification could be improved through statistical modelling, data collection, or adaptation of the assessment process. We develop a framework for end-to-end quantification of uncertainty, integrating uncertainty estimates from individual stages of the assessment process. Finally, we provide research recommendations to better quantify and reduce uncertainty, to lower future ORD consenting risk. These recommendations extend beyond the UK and could improve impact assessments in other countries with different legislative frameworks.

Synergistic use of UAV surveys, satellite tracking data, and mark‐recapture to estimate abundance of elusive species

AbstractEstimating population abundance is central to many ecological studies and important in conservation planning. Yet the elusive nature of many species makes estimating their abundance challenging. Abundance estimates of sea turtles, marine birds, and seals are usually made when breeding adults are ashore, while life stages spent at sea, including as juveniles, are often poorly sampled. We used a combination of high‐resolution satellite tracking (Fastloc‐GPS), uncrewed aerial vehicle (UAV) surveys, and capture‐mark‐recapture approaches to assess the abundance of immature hawksbill (Eretmochelys imbricata) and green turtles (Chelonia mydas) in a tidal lagoon of the Chagos Archipelago (Indian Ocean). We captured, marked, and released 50 turtles (48 hawksbill and 2 green turtles) prior to UAV surveys and used satellite tracking data from 27 immature turtles (25 hawksbill and 2 green turtles) to refine the estimated numbers of marked turtles available for resighting and those likely to have emigrated from the study area. We estimated a total of 339 turtles in the lagoon with a density variation at different tidal heights between 265 turtles km−2 at high water and 499 turtles km−2 at low water. Of these, 91% were hawksbills and 9% were green turtles. These hawksbill densities are the highest reported among 17 foraging sites recorded around the world and likely reflect successful long‐term protection of turtles in the Chagos Archipelago.

Avian influenza overview December 2022 - March 2023.

Between 3 December 2022 and 1 March 2023 highly pathogenic avian influenza (HPAI) A(H5N1) virus, clade 2.3.4.4b, was reported in Europe in domestic (522) and wild (1,138) birds over 24 countries. An unexpected number of HPAI virus detections in sea birds were observed, mainly in gull species and particularly in black-headed gulls (large mortality events were observed in France, Belgium, the Netherlands, and Italy). The close genetic relationship among viruses collected from black-headed gulls suggests a southward spread of the virus. Moreover, the genetic analyses indicate that the virus persisted in Europe in residential wild birds during and after the summer months. Although the virus retained a preferential binding for avian-like receptors, several mutations associated to increased zoonotic potential were detected. The risk of HPAI virus infection for poultry due to the virus circulating in black-headed gulls and other gull species might increase during the coming months, as breeding bird colonies move inland with possible overlap with poultry production areas. Worldwide, HPAI A(H5N1) virus continued to spread southward in the Americas, from Mexico to southern Chile. The Peruvian pelican was the most frequently reported infected species with thousands of deaths being reported. The reporting of HPAI A(H5N1) in mammals also continued probably linked to feeding on infected wild birds. In Peru, a mass mortality event of sea lions was observed in January and February 2023. Since October 2022, six A(H5N1) detections in humans were reported from Cambodia (a family cluster with 2 people, clade 2.3.2.1c), China (2, clade 2.3.4.4b), Ecuador (1, clade 2.3.4.4b), and Vietnam (1, unspecified clade), as well as two A(H5N6) human infections from China. The risk of infection with currently circulating avian H5 influenza viruses of clade 2.3.4.4b in Europe is assessed as low for the general population in the EU/EEA, and low to moderate for occupationally or otherwise exposed people.

Estimating populations of dense animals using acoustics

Acoustic censusing methods offer an efficient and noninvasive method to monitor populations of dense aggregations of animals on a large scale. Large, dense colonies of bats are difficult to census, and current monitoring methods are often invasive and time consuming. Using acoustic recordings collected during emergence, we developed a linear regression model that uses multiple measures of acoustic energy to estimate the number of bats per second. The model was trained using data from audio recordings collected at seven cave locations and two species of bats, Brazilian free-tailed bats (Tadarida brasiliensis) and gray bats (Myotis grisenscens). Visual counts from synchronized thermal videos were used to ground truth the model. Environmental factors, such as distance from microphone to emergence, were integrated to create flexible model that is widely applicable across species and habitats. This method can be extended to other species that vocalize in large aggregations, such as sea birds and frogs. Ultimately, this model can be integrated into autonomous monitoring stations to monitor species in real time. It is imperative that efficient and noninvasive methods for monitoring bat populations are developed to increase population data available for informing crucial management decisions.

Pacific Salmon Strategy Initiative will conserve marine birds with fishery closures and gillnet license retirements

Due to their lack of selectivity, entanglement of marine birds, along with a wide range of other species, in gillnets is a conservation issue worldwide. In Pacific Canada, mortality from incidental take in salmon gillnet fisheries has been identified as one of the largest sources of human-induced mortality for marine diving birds, a conservation concern impacting both local breeders and visiting migrants. In 2021, to address historic declines in Pacific salmon populations, Fisheries and Oceans Canada introduced the Pacific Salmon Strategy Initiative (PSSI). The PSSI is transformative and includes long-term commercial closures and a license retirement program. The PSSI will move the fishery away from gillnets towards more selective fishing methods with lower release mortality of non-target salmon species. Gillnet fishery closures and license retirements related to new policies under the PSSI will reduce entanglement mortality of migratory marine birds and species at risk from Canada and elsewhere, as a significant conservation byproduct. Closures and license retirements are the most effective tools to reduce bird entanglement mortality in gillnets because of the lack of technical solutions to reduce bycatch and the challenges to fund and develop robust, long-term, interdepartmental monitoring programs to inform the management of incidental take.

Exploring the Astrovirome of Shellfish Matrices Using Nanopore Sequencing.

Astroviruses are important human enteric pathogens transmissible with contaminated food and water. Astroviruses have also been identified in mammals, birds, lower vertebrates and invertebrates. The genetic diversity of human and animal astroviruses poses a challenge for diagnostics and taxonomy. As a proof of concept, we used a panastrovirus consensus primer set, able to amplify in a nested RT-PCR protocol a 400-nt-long fragment of the RNA-dependent RNA polymerase of most members of the Astroviridae family, in conjunction with a nanopore sequencing platform, to generate information on the astrovirome in filter-feeding mollusks. Amplicons generated from bivalve samples were used to generate libraries for deep sequencing. In three samples, only one unique RdRp sequence type was obtained. However, in seven samples and in three barcodes with eleven pooled samples, we identified a variety of known and unknown RdRp sequence types, in most cases distantly related to astrovirus sequences available in the databases. In total, 37 different sequence contigs were generated. Avian-origin astrovirus sequences were predominant, likely due to contamination of shellfish harvesting waters by marine birds. Astroviruses of the aquatic eco-system were also identified, whereas human astroviruses were not detected.

SnapperGPS: Open Hardware for Energy-Efficient, Low-Cost Wildlife Location Tracking with Snapshot GNSS

Location tracking with global navigation satellite systems (GNSS), such as the GPS, is used in many applications, including the tracking of wild animals for research. Snapshot GNSS is a technique that only requires milliseconds of satellite signals to infer the position of a receiver. This is ideal for low-power applications such as animal tracking. However, there are few existing snapshot systems, none of which is open source. To address this, we developed SnapperGPS, a fully open-source, low-cost, and low-power location tracking system designed for wildlife tracking. SnapperGPS comprises three parts, all of which are open-source: (i) a small, low-cost, and low-power receiver; (ii) a web application to configure the receiver via USB; and (iii) a cloud-based platform for processing recorded data. This paper presents the hardware side of this project. The total component cost of the receiver is under $30, making it feasible for field work with restricted budgets and low recovery rates. The receiver records very short and low-resolution samples resulting in particularly low power consumption, outperforming existing systems. It can run for more than a year on a 40 mAh battery. We evaluated SnapperGPS in controlled static and dynamic tests in a semi-urban environment where it achieved median errors of 12 m. Additionally, SnapperGPS has already been deployed for two wildlife tracking studies on sea turtles and sea birds.

A Novel Pathogenic Avipoxvirus Infecting Vulnerable Cook's Petrel (Pterodroma cookii) in Australia Demonstrates a High Genomic and Evolutionary Proximity with South African Avipoxviruses.

Avipoxviruses are assumed to be restricted to avian hosts and are considered to be important viral pathogens that may impact the conservation of many vulnerable or endangered birds. Recent reports of avipoxvirus-like viruses from reptiles suggest that cross-species transmission may be possible within birds and other species. Most of the avipoxviruses in wild and sea birds remain uncharacterized, and their genetic variability is unclear. Here, cutaneous pox lesions were used to recover a novel, full-length Cook's petrelpox virus (CPPV) genome from a vulnerable Cook's petrel (Pterodroma cookii), and this was followed by the detection of immature virions using transmission electron microscopy (TEM). The CPPV genome was 314,065 bp in length and contained 357 predicted open-reading frames (ORFs). While 323 of the ORFs of the CPPV genome had the greatest similarity with the gene products of other avipoxviruses, a further 34 ORFs were novel. Subsequent phylogenetic analyses showed that the CPPV was most closely related to other avipoxviruses that were isolated mostly from South African bird species and demonstrated the highest sequence similarity with a recently isolated flamingopox virus (88.9%) in South Africa. Considering the sequence similarity observed between CPPV and other avipoxviruses, TEM evidence of poxvirus particles, and phylogenetic position, this study concluded that CPPV is a distinct candidate of avipoxviruses. IMPORTANCE Emerging viral disease is a significant concern with potential consequences for human, animal, and environmental health. Over the past several decades, multiple novel viruses have been found in wildlife species, including birds, and they can pose a threat to vulnerable and endangered species. Cook's petrel is currently listed as vulnerable. The threats to the species vary, but are, to a large degree, due to anthropogenic impacts, such as climate change, habitat loss, pollution, and other disturbances by humans. Knowledge of viral pathogens, including poxvirus of Cook's petrel is currently virtually nonexistent.

Infection patterns of helminth community in black rockcod Notothenia coriiceps in West Antarctica over a 6-year term.

Patterns of the rockcod Notothenia coriiceps infection with helminths were analysed to understand the dynamics of parasite communities in this Antarctic fish and to test their stability over time. The study was performed using helminth samples collected from 183 N. coriiceps in 2014-2015 and 2020-2021 in the vicinity of the Ukrainian Antarctic station (UAS) "Akademik Vernadsky", Galindez Island, Argentine Islands, West Antarctica. Overall, 25 helminth taxonomical categories (nine trematodes, four cestodes, five nematodes, and seven acanthocephalans) were subjected to analysis. A direct comparison of the helminth population characteristics showed that nine species significantly changed their infection parameters during the 6years between the samples. Seven of them (Pseudoterranova sp., Contracaecum sp., Ascarophis nototheniae, monolocular metacestodes, bilocular metacestodes, Metacanthocephalus rennicki, and Diphyllobothrium sp.) were found to have a significant impact on the differences between helminth infracommunities in 2014-2015 and 2020-2021. Most studied patterns of helminth component community appeared to show a stable tendency, and observed fluctuations were close to the steady trend. Slight but significant changes in the infection patterns observed in this study might have been caused by changes in the populations of intermediate, paratenic, and definitive hosts of helminths (marine invertebrates, mammals, and birds), which participate in helminth transmission in Antarctic ecosystems.

Contrasting patterns of population structure of Bulwer’s petrel (Bulweria bulwerii) between oceans revealed by statistical phylogeography

The patterns of population divergence of mid-latitude marine birds are impacted by only a few biogeographic barriers to dispersal and the effect of intrinsic factors, such as fidelity to natal colonies or wintering grounds, may become more conspicuous. Here we describe, for the first time, the phylogeographic patterns and historical demography of Bulwer’s petrel Bulweria bulwerii and provide new insights regarding the drivers of species diversification in the marine environment. We sampled Bulwer’s petrels from the main breeding colonies and used a statistical phylogeography approach based on surveying nuclear and mitochondrial loci (~ 9100 bp) to study its mechanisms of global diversification. We uncovered three highly differentiated groups including the Western Pacific, the Central Pacific and the Atlantic. The older divergence occurred within the Pacific Ocean, ca. 850,000 ya, and since then the W Pacific group has been evolving in isolation. Conversely, divergence between the Central Pacific and Atlantic populations occurred within the last 200,000 years. While the Isthmus of Panama is important in restricting gene flow between oceans in Bulwer’s petrels, the deepest phylogeographic break is within the Pacific Ocean, where oceanographic barriers are key in driving and maintaining the remarkable structure found in this highly mobile seabird. This is in contrast with the Atlantic, where no structure was detected. Further data will provide insights regarding the extent of lineage divergence of Bulwer’s petrels in the Western Pacific.

Arctic marine ecosystems face increasing climate stress

Arctic warming is occurring at a much faster rate than in other parts of the globe, with potentially devastating consequences for the diverse array of species within ecologically and biologically sensitive areas in the Arctic marine region. However, climate change research in this region is sparse compared to other biomes, hindering conservation efforts. In this article, we review and synthesize the available literature to understand the observed and potential impacts of climate change on different species and ecosystems in the Arctic marine region. We reviewed 253 articles reporting changes in species and ecosystems in the Arctic marine region in response to climate change (225 studies documenting observed impacts and 28 reporting predicted impacts). The review revealed that most research effort has been concentrated in only a subset of Arctic Large Marine Ecosystems (LMEs) (5/18 LMEs), with the majority of Arctic LMEs being poorly studied. The majority of the reviewed papers focused on marine mammals (19%) followed by microalgae (17%). A number of studies documented variability in planktonic communities (microalgae, macroalgae, zooplankton), resulting in alteration in ecosystem structure and function. Reproductive failure, decline in populations, and changes in diet composition, behavior, and breeding biology were reported for sea birds and mammals. Further, shifts in spatial distribution of fishes were observed by several studies. This review provides persuasive evidence that multiple climate change indices in the Arctic region are changing, with impacts on almost all components of marine biotic ecosystems (from plankton to top predators). We identify a number of gaps (such as limited inventory of biota, robustness of data supporting climate change indices on an Arctic Ocean scale, and climate suitability assessment for all species) in climate change research in Arctic marine ecosystems where future studies can help to quantify the impacts of climate change on species and ecosystems and advance understanding for their adaptive management.

Avian flu threatens Neotropical birds

A highly pathogenic avian influenza virus (HPAIv) that spread through the Holarctic region in 2022, affecting millions of birds (1), has reached South America. The disease threatens marine and terrestrial birds, including endangered species. Real-time information gathering and steps to prevent the spread of disease will be required to mitigate this outbreak.

Avoiding grey seal depredation in the Baltic Sea while increasing catch rates of cod

The escalating conflict between gillnet fisheries and the growing seal populations in Baltic Sea has increased the need to reduce direct effects in form of reduced or damaged catches. This study describes the design and catch rates of 20 different seal safe cod (Gadus morhua) pot designs suitable for the Baltic Sea. Catches in numbers of individuals and total catch weight were recorded in 2762 pot hauls with pots varying in terms of size, shape, number of entrances, entrance length, pot placement in relation to the sea floor and catch holding chamber. The aim was to investigate the how the different designs and soak times affected the catch rates under similar conditions. Volume and soak time was significantly positively correlated with catch rates and round bottom-standing pots with a fish holding chamber, funnels and 5 entrances had the highest catch rates. The findings are important for the development of cod pots, which can allow the fishers to continue fishing in areas of high seal density with static gear and hence low-carbon emission and minimum bycatch of marine mammals and sea birds.

Soilscapes and geoenvironments at Stansbury Peninsula, Nelson Island, maritime Antarctica

Considering that the extreme variation in Antarctica’s climate has important effects on soil properties and distribution, characterizing and mapping surface landforms in ice-free areas is strategic. We aimed to evaluate the pedogenetic processes, as well as classify and map the different types of soils at Stansbury Peninsula (ST), Nelson Island. In order to stratify the whole environment area of the Peninsula, we used the geosystems’ approach, identifying the landscape unit of geoenvironment. We mapped geoenvironments and soils, performing physical–chemical and mineralogical characterizations. Our results suggest that compared to other islands in the South Shetlands, the active layer at Stansbury is thin, so that it is more susceptible to climate change, representing a Key monitoring site for climate change studies. The northwestern area of Stansbury Peninsula is the preferential area for marine birds, mammals nesting and resting places. Hence, higher values of nutrients are recorded in their soils. The climate at ST is harsh due to strong northwest polar winds, which promote widespread wind ablation, erosion and contribute to physical weathering, with a high prevalence of periglacial processes under wet conditions, which is typical of this zone of South Shetlands areas, exposed to the Drake passage. Soils at ST are predominantly skeletal, gravelly, with little organic matter and little fauna activity, poor vegetation cover, and high levels of base saturation suggesting limited weathering of volcanic rocks. Soils display a strong characteristic of physical weathering due freezing and thawing process, resulting in typical patterned ground (cryoturbation forming Cryosols), under active periglacial processes. The unique proglacial and periglacial geoenvironments of Stansbury are influenced by melting water and widespread snow banks, under wet polar oceanic climate condition.

First sighting of a pelagic seabird entangled in a disposable COVID-19 facemask in the Mediterranean Sea

Seabirds are increasingly recognized as important bio-indicators of marine ecosystems that are useful in assessing environmental disturbance on the marine biota. Over the period 2020-22 and during the first national systematic recording of the sea waters surrounding the Republic of Cyprus, we recorded the spatio-temporal presence, abundance and behaviour of seabirds using the ESAS (European Seabirds At Sea) methodology. Here we present the observation of an accidentally entangled pelagic seabird in COVID-19 material which to the best of our knowledge is the first incident in the Mediterranean Basin. The systematic recording of entangled marine birds in personal protective equipment (PPE) used to prevent COVID-19 transmission worldwide seems to be of crucial importance for one of the most important emerging threats for the conservation of seabirds at global scale.

A marine bird (sulidae, Aves) from the Langhian (middle Miocene) of Penedo beach (Setúbal Peninsula—SW Portugal) and its paleoenvironmental context

The fossil remains of birds from the Miocene of Portugal are scarce, encompassing a total of twelve specimens, from nine paleontological outcrops located in Leiria (central Portugal), southern sector of Setúbal Peninsula and along the lower Tagus Basin. This study focuses on a new specimen found in the Praia do Penedo Norte (Sesimbra) coastal cliff corresponding to a coracoid bone, attributed to Morus sp., a sulid bird, biostratigraphically framed by calcareous nannofossils within the middle Miocene (Langhian).

Assessing individual movement, habitat use, and behavior of non-breeding marine birds in relation to prey availability in the US Atlantic

Resource availability is a key factor driving marine bird movements and distributions, but direct information on prey availability is difficult to obtain at relevant scales. We present novel methods for describing multi-scale trophic associations, combining movement analyses of marine birds with estimates of forage fish surface aggregations from digital aerial survey data and species occupancy from bottom trawl survey data. We analyzed satellite telemetry data from northern gannets Morus bassanus, red-throated loons Gavia stellata, and long-tailed ducks Clangula hyemalis in the US Atlantic during the non-breeding period. Using discrete-time hidden Markov models to distinguish area-restricted (i.e. putative foraging) from transit movements, we examined how environmental factors influence movement, and how forage fish species distributions and surface aggregations influence habitat use by gannets and loons that have greater dietary reliance. Our results suggest that chlorophyll a concentration significantly affected movement behavior across species, highlighting the importance of higher-productivity areas around estuaries during colder months when regional productivity is low. Though variable across species and seasons, spatial cross-correlation analysis revealed that herring species (Family Clupeidae), including Atlantic menhaden Brevoortia tyrannus, may be important resources; it also showed positive spatial correlations with forage fish aggregations. This suggests that prey patch dynamics and factors driving aggregation formation may be as important as species composition. However, spatial patterns were generally low (<0.3), suggesting a mismatch in spatiotemporal resolution, exemplifying the challenges in quantifying trophic relationships in marine systems. Disentangling predator-prey relationships is critical to understanding the mechanisms driving marine bird behavior in rapidly changing marine systems.

A review of inorganic contaminants in Australian marine mammals, birds and turtles

Environmental context Metal concentrations can build up to potentially harmful levels in marine mammals as they are at the top of the food chain. This review summarises the information available on metal concentrations in marine mammals, birds and turtles from around Australia. Despite large data gaps, the available data suggest that metal concentrations are similar to those encountered in other regions of the world. Abstract A comprehensive compilation of the published data for trace element concentrations (metals and metalloids) in Australian marine mammals, birds and turtles is presented. The majority of studies have relied on the utilisation of opportunistically collected samples, animal strandings and bycatch. This has resulted in large gaps in geographical, temporal and species coverage data. For instance, little or no data are available for cetaceans in New South Wales or the Northern Territory, and out of 14 endemic species of dolphins, data only exist for seven species. The aforementioned data gaps make it hard to identify statistically significant trends, a problem compounded by data being reported in the form of ranges without raw data. Trace element concentrations measured in various marine species and their tissue types are extremely variable, with ranges typically spanning several orders of magnitude, but are generally comparable with international data. Trends in contaminant concentrations with tissue type follow generally accepted patterns of behaviour for higher organisms, with the highest mercury concentrations in liver and cadmium in kidney tissues. Herbivores have lower contaminant loadings than carnivores, reflecting the importance of diet, and there are identifiable age-related trends for elements such as mercury. The lack of supporting pathology on dead and stranded animals and data on specimens from uncontaminated locations restrict conclusions on organism health impacts. There have been some attempts to use non-invasive sampling of indicator tissues such as fur, bristle and feathers. However, it is currently difficult to extrapolate these data to estimate contaminant concentrations in major organs. Recommendations for future investigations are made.