Sea Buckthorn Cultivars Research Articles

Features of Bioecology of Sea Buckthorn Flies Rhagoletis batava Hering in the Central Non-Chernozem Region of Russia

Sea buckthorn fly Rhagoletis batava Hering (Diptera; Tephritidae) was first recorded in the Central Non-Chernozem Region of Russia in the early 21st century. The pest adapted in a short time and there are periodically massive outbreaks of reproduction. In some years, this phytophage damages more than 80% of susceptible sea buckthorn cultivars’ fruits. However, the bioecology of R. batava in the specified area has not been studied, which hampers the timely implementation of protective measures and ensuring of their high efficiency. The beginning of flight of flies, oviposition, and larval hatching strongly depend on weather conditions and they occur with a difference in the calendar period up to 25–30 days in different years. In the years of research, the sum of effective temperatures above 10°C at the beginning of flight of flies ranged from 252.1 to 319°С, from 339.5 to 390.3°C at the beginning of oviposition, and from 428.3 to 471.0°C at the beginning of larval hatching. The duration of the summer period in the exploration area ranges from 48 to 57 days. To track the dynamics of the summer and to more accurately determine the beginning of the individual phases of development of the pest, it is necessary to use yellow two-sided glue traps and fruit inspection under a microscope.

Comprehensive analysis of differentially expressed genes reveals the molecular response to elevated CO2 levels in two sea buckthorn cultivars

Atmospheric carbon dioxide (CO2) concentration increases every year. It is critical to understand the elevated CO2 response molecular mechanisms of plants using genomic techniques. Hippophae rhamnoides L. is a high stress resistance plant species widely distributed in Europe and Asia. However, the molecular mechanism of elevated CO2 response in H. rhamnoides has been limited. In this study, transcriptomic analysis of two sea buckthorn cultivars under different CO2 concentrations was performed, based on the next-generation illumina sequencing platform and de novo assembly. We identified 4740 differentially expressed genes in sea buckthorn response to elevated CO2 concentrations. According to the gene ontology (GO) results, photosystem I, photosynthesis and chloroplast thylakoid membrane were the main enriched terms in ‘xiangyang’ sea buckthorn. In ‘zhongguo’ sea buckthorn, photosynthesis was also the main significantly enriched term. However, the number of photosynthesis related differentially expressed genes were different between two sea buckthorn cultivars. Our GO and pathway analyses indicated that the expression levels of the transcription factors WRKY, MYB and NAC were significantly different between the two sea buckthorn cultivars. This study provides a reliable transcriptome sequence resource and is a valuable resource for genetic and genomic researches for plants under high CO2 concentration in the future.

Role of Flavonols and Proanthocyanidins in the Sensory Quality of Sea Buckthorn (Hippophaë rhamnoides L.) Berries.

Sensory profile, flavonols, proanthocyanidins, sugars, and organic acids were investigated in purees of six sea buckthorn (Hippophaë rhamnoides) cultivars. The sensory profiles of the purees were dominated by intense sourness followed by astringency and bitterness due to the high content of malic acid. Malic acid and isorhamnetin glycosides, especially isorhamnetin-3-O-sophoroside-7-O-rhamnoside, had close association with the astringent attributes in the different purees, whereas some of the known astringent compounds such as proanthocyanidin dimers and trimers or quercetin glycosides, had less impact. Moreover, the ratios between contents of acids and phenolic compounds were more important predictors of bitterness than the individual variables alone. Astringency and bitterness are important sensory factors for the consumer acceptance of sea buckthorn products. The current study provides new knowledge on the correlations between sensory properties and composition and supports industrial utilization of the sea buckthorn berries.

Development of RNA-Seq SSR Markers and Application to Genetic Relationship Analysis among Sea Buckthorn Germplasm

Sea buckthorn (Hippophae rhamnoides) is an ecologically and economically valuable species that has been widely cultivated as a new berry crop rich in nutritional and medicinal compounds. RNA Sequencing (RNA-Seq) simple sequence repeat (SSR) markers were developed to evaluate the genetic relationships among 91 plants of 31 cultivars from two subspecies, mongolica and sinensis, as well as intraspecific hybrids between them. A total of 7540 RNA-Seq SSRs were identified as potential molecular markers, in which AG/CT (27.57%) was the most abundant unit type. AT/AT (9.93%), and AAG/CTT (11.95%) are the other main repeat motifs. A total of 110 primer pairs were randomly selected for validation of amplification. Seventeen SSR loci, located in genes encoding metabolic processes and cellulose synthases, were identified to be polymorphic among different sea buckthorn cultivars. These SSR loci generated 48 alleles, ranging from 2 to 5 per locus. Cluster analysis based on the proportion of shared alleles and unweighted pair group method with arithmetic average (UPGMA) algorithm divided all the genotypes into two main groups, with all of the ssp. sinensis cultivars (native to China) and hybrids in one group and ssp. mongolica cultivars (introduced from Russia) in the other group, which was in good agreement with their taxonomic classification. The RNA-Seq SSRs developed in this study have a potential use in the conservation of sea buckthorn germplasm and marker-assisted breeding (MAB).

NMR metabolomics demonstrates phenotypic plasticity of sea buckthorn (Hippophaë rhamnoides) berries with respect to growth conditions in Finland and Canada.

The berries of sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) cultivars 'Terhi' and 'Tytti' were studied with respect to their growth location, 60° and 68°N latitude in Finland and 46°N in Canada, using 1H NMR metabolomics. The berries of 'Terhi' were characterised by stronger signals of quinic acid, while 'Tytti' had higher levels of O-ethyl β-d-glucopyranoside. The metabolic profile of the northernmost berries was distinctly different from those grown in southern Finland or Canada. Berries from northern Finland had relatively higher levels of quinic acid, glucose, l-quebrachitol and ascorbic acid. Ethyl glucoside was shown to accumulate by several fold at the late stage of maturation in the south as it correlated with degree days (r=0.63) and global radiation (r=0.59), but not in the north. The variance in the composition of the sea buckthorn berries demonstrates plasticity in the acclimatisation to growth environments.

Metabolite profiling and expression analysis of flavonoid, vitamin C and tocopherol biosynthesis genes in the antioxidant-rich sea buckthorn (Hippophae rhamnoides L.)

In this study, phenolic compounds were analyzed in developing berries of four Canadian grown sea buckthorn (Hippophae rhamnoides L.) cultivars (‘RC-4’, ‘E6590’, ‘Chuyskaya’ and ‘Golden Rain’) and in leaves of two of these cultivars. Among phenolic acids, p-coumaric acid was the highest in berries, while gallic acid was predominant in leaves. In the flavonoid class of compounds, myricetin/rutin, kaempferol, quercetin and isorhamnetin were detected in berries and leaves. Berries of the ‘RC-4’ cultivar had approximately ⩾2-fold higher levels of myricetin and quercetin at 17.5mg and 17.2mg/100g FW, respectively, than the other cultivars. The flavonoid content in leaves was considerably more than in berries with rutin and quercetin levels up to 135mg and 105mg/100g FW, respectively. Orthologs of 15 flavonoid biosynthesis pathway genes were identified within the transcriptome of sea buckthorn mature seeds. Semi-quantitative RT-PCR analysis of these genes in developing berries indicated relatively higher expression of genes such as CHS, F3′H, DFR and LDOX in the ‘RC-4’ cultivar than in the ‘Chuyskaya’ cultivar. Vitamin C levels in ripened berries of the Canadian cultivars were on the high end of the concentration range reported for most other sea buckthorn cultivars. Orthologs of genes involved in vitamins C and E biosynthesis were also identified, expanding the genomic resources for this nutritionally important plant.

Effect of growth environment on the gene expression and lipids related to triacylglycerol biosynthesis in sea buckthorn (Hippophaë rhamnoides) berries

Sea buckthorn (Hippophaë rhamnoides) has numerous health properties and an important potential as a functional food. As one important healthy feature, the beneficial effects of sea buckthorn seed and mesocarp oil to humans have been demonstrated in clinical trials. The seed oil is rich in linoleic (18:2n−6) and α-linolenic (18:3n−3) acids, while the berry mesocarp oil is rich in palmitoleic (16:1n−7) and vaccenic acids (18:1n−7), up to 40% and 10%, respectively.We studied the effect of extreme growth conditions to triacylglycerol content and composition and the related gene expression of two Scandinavian sea buckthorn cultivars. The plants were cultivated at two locations that were very different climate-wise (latitudes 60° N and 68° N) and their berry samples of two years were analyzed. The composition of seed oil and mesocarp oil differed between the growth locations. The amount of linoleic acid increased in both oils in the northern extreme climate. Additionally, the two cultivars differed from each other in mesocarp oil composition. Gene expression levels of genes related to triacylglycerol biosynthesis were under transcriptional control during berry development, while adjustment to growth environment was likely to be under post-transcriptional control. Expression of the genes encoding diacylglycerol acyltransferase (DGAT) increased most prominently in seeds towards berry ripening. The study showed that growth conditions have a significant effect on seed oil biosynthesis and composition.

Root morphology and cluster root formation by seabuckthorn (Hippophaë rhamnoides L.) in response to nitrogen, phosphorus and iron deficiency

The aims were to investigate effects of availability of nitrogen (N), phosphorus (P) and iron (Fe) on root properties of seabuckthorn (Hippophae rhamnoides) and to test the hypothesis that seabuckthorn is able to form cluster roots (CRs). Two sources of seabuckthorn were used: the seabuckthorn cultivar BHi10726 originating from a breeding programme based on H.r. ssp mongolica and carried out in rich agricultural field soil in the black earth (chernozem) region of Russia and the seabuckthorn accession named Pk originating in a natural population of H.r. ssp turkestanica in the mountainous region of northern Pakistan. Three cultivation systems giving different water availabilities were used at two levels each of N, P and Fe. Root morphology of seedlings and clones was characterized and metabolite content in extracts of young and old CRs of Pk was analyzed by proton nuclear magnetic resonance spectroscopy. Availability of N affected growth and distribution of biomass between shoot and root, while P and Fe deficiency modified root system architecture towards more lateral roots. Densely positioned rootlets with a determinate type of growth consistent with the definition of CR were observed under low P and low Fe. Pk formed on average 12 CRs per plant, which was 3 to 4-fold higher compared to BHi10726 also when normalized per root length. Malate and glycine were most abundant of the organic acids and amino acids, respectively, and decreased in old CRs. Seabuckthorn has the ability to form cluster roots especially in Pk and under deficiency of P and Fe. The two sources of seabuckthorn with different histories showed distinctly different root system architectures. The high contents of malate and glycine and their decrease in old CRs may reflect roles in CR metabolism.

Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn (Hippophaë rhamnoides L.) Berries.

The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophaë rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids), UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as well spectrophotometric method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds β-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6.19 and 23.91 mg/100 g fresh weight (fw) (p < 0.05). The major polyphenol group identified in berries was flavonols (mean content of 311.55 mg/100 g fw), with the structures of isorhamnetin (six compounds), quercetin (four compounds), and kaempferol (one compound) glycosides. Examined sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52.86 to 130.97 mg/100 g fw (p < 0.05).

A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers.

DNA fingerprinting is both a popular and important technique with several advantages in plant cultivar identification. However, this technique has not been used widely and efficiently in practical plant identification because the analysis and recording of data generated from fingerprinting and genotyping are tedious and difficult. We developed a novel approach known as a cultivar identification diagram (CID) strategy that uses DNA markers to separate plant individuals in a more efficient, practical, and referable manner. A CID was manually constructed and a polymorphic marker was generated from each polymerase chain reaction for sample separation. In this study, 67 important sea buckthorn cultivars cultivated in China were successfully separated with random amplified polymorphic DNA markers using the CID analysis strategy, with only seven 11-nucleotide primers employed. The utilization of the CID of these 67 sea buckthorn cultivars was verified by identifying 2 randomly chosen groups of cultivars among the 67 cultivars. The main advantages of this identification strategy include fewer primers used and separation of all cultivars using the corresponding primers. This sea buckthorn CID was able to separate any sea buckthorn cultivars among the 67 studied, which is useful for sea buckthorn cultivar identification, cultivar-right-protection, and for the sea buckthorn nursery industry in China.

Comparison of selected aroma compounds in cultivars of sea buckthorn (Hippophae rhamnoides L.)

AbstractThirteen cultivars of sea buckthorn (Hippophae rhamnoides L.) berries: Aromat, Botanicky, Buchlovicky, Hergo, Krasavica, Leicora, Ljubitelna, Pavlovsky, Peterbursky, Sluničko, Trofinovsky, Vitaminnaja and Velkoosecky, were tested for the content of volatile aroma compounds using gas chromatography with the solid phase microextraction method during two consequent years (2012- 2013). In total, 69 volatile compounds were identified: 26 alcohols, 12 aldehydes, 11 ketones, 9 acids and 11 esters. Based on principal component analysis, 18 most relevant compounds, best representing the variability of the whole system and suitable for the discrimination of the samples, were selected from all compounds identified. These compounds were then compared using the analysis of variance to confirm differences between the samples. Significant (p &lt; 0.05) differences were found in the varieties in both years, Krasavica and Sluničko cultivars were found to be quite different from other varieties, being rich in the compounds identified and containing most of the selected compounds. Variability within the cultivars (between picking years) was low or not significant.

Changes in content of major phenolic compounds during leaf development of sea buckthorn (Hippophaë rhamnoides L.)

Content of total phenolic compounds and antioxidant capacity (FRAP) were investigated in the leaves of three sea buckthorn (Hippophaë rhamnoides L.) cultivars: ‘Otradnaja’, ‘Gibrid Pertjika’ and ‘Ljubitelskaja’, at different dates. In addition, major phenolic compounds (catechin, kaempferol, quercetin, epigallocatechin, kaempferol-3-O-glucoside, quercetin-3-O-galactoside, isorhamnetin-3-O-glucoside, rutin, gallic acid, procyanidin monomer glycoside, procyanidin dimer aglycone and hydrolyzable tannins I–III) were determined in ‘Ljubitelskaja’. Antioxidant capacity and the content of total phenolic compounds fluctuated during April, May and June, and then increased until the end of July, when the highest values were observed. Total phenolic compounds were strongly correlated with FRAP. Levels were generally higher in ‘Ljubitelskaja’ than in ‘Otradnaja’ and ‘Gibrid Pertjika’. In ‘Ljubitelskaja’, hydrolyzable tannins I–III occurred in higher amounts than did any of the other studied phenolic compounds. The developmental stage of the leaves (harvesting date) had a strong influence on content of phenolic compounds and should be carefully considered when harvesting sea buckthorn leaves for different purposes.

Harvest Moon seabuckthorn

Schroeder, W. R., Soolanayakanahally, R. Y. and Naeem, H. A. 2014. Harvest Moon seabuckthorn. Can. J. Plant Sci. 94: 1309–1312. Harvest Moon is a female, winter-hardy seabuckthorn (Hippophae rhamnoides L. ssp. mongolica) cultivar released by Agriculture and Agri-Food Canada (AAFC), Agroforestry Development Centre (ADC). It is an early-maturing relatively thornless seabuckthorn with reddish-orange berries. The berries are sour-sweet, large with long pedicels, which facilitates hand harvesting. Harvest Moon is adapted for fruit orchards in the Canadian Prairies and northern Great Plains of the United States. The name Harvest Moon was chosen as it represents the large reddish-orange oblong berries characteristic of the cultivar.

Harvest Moon seabuckthorn

Schroeder, W. R., Soolanayakanahally, R. Y. and Naeem, H. A. 2014. Harvest Moon seabuckthorn. Can. J. Plant Sci. 94: 1309-1312. Harvest Moon is a female, winter-hardy seabuckthorn (Hippophae rhamnoides L. ssp. mongolica) cultivar released by Agriculture and Agri-Food Canada (AAFC), Agroforestry Development Centre (ADC). It is an early-maturing relatively thornless seabuckthorn with reddish-orange berries. The berries are sour-sweet, large with long pedicels, which facilitates hand harvesting. Harvest Moon is adapted for fruit orchards in the Canadian Prairies and northern Great Plains of the United States. The name Harvest Moon was chosen as it represents the large reddish-orange oblong berries characteristic of the cultivar.

Применение метода дифференциальной сканирующей калориметрии для идентификации происхождения облепихового масла Алтайского края

Abstract: The composition of lipids derived by extraction with Freon 22 and enzymatic hydrolysis from berries, berry shells, and seeds of the Chuy sea buckthorn cultivar has been studied. The fatty acid composition and acid and peroxide values of the samples have been analyzed; the differential scanning calorimetry (DSC) melting curves have been examined. The DSC method has been found to be appropriate for determining the origin of raw materials and the production method for sea buckthorn oil. Keywords: sea buckthorn oil, the Altai Territory, production method, differential scanning calorimetry 1. INTRODUCTION Sea buckthorn berries are rich in vitamins, carotenoids, flavonoids, proteins, antioxidants, amino acids, fatty acids, and phytosterols [1]. The most valuable component of sea buckthorn berries is their oil. The oil from the sea buckthorn pulp and seeds is characterized by a high content of lipids, including tocopherols, 3tocotrienols, carotenoids, and ω-3 and ω-6 polyunsaturated fatty acids [2, 3]. The composition of the sea buckthorn seeds and pulp varies in accordance with the subspecies, cultivar, soil and climate conditions, origin, cultivation activities, harvesting time, and extraction method [3]. The aim of this study is to explore the possibility of identifying samples of sea buckthorn oil derived from different parts of sea buckthorn berries by differential scanning calorimetry (DSC).

Fatty acids in berry lipids of six sea buckthorn (Hippophae rhamnoides L., subspecies carpatica) cultivars grown in Romania

BackgroundA systematic mapping of the phytochemical composition of different sea buckthorn (Hippophae rhamnoides L.) fruit subspecies is still lacking. No data relating to the fatty acid composition of main lipid fractions from the berries of ssp. carpatica (Romania) have been previously reported.ResultsThe fatty acid composition of the total lipids (oils) and the major lipid fractions (PL, polar lipids; FFA, free fatty acids; TAG, triacylglycerols and SE, sterol esters) of the oils extracted from different parts of six sea buckthorn berry subspecies (ssp. carpatica) cultivated in Romania were investigated using the gas chromatography-mass spectrometry (GC-MS). The dominating fatty acids in pulp/peel and whole berry oils were palmitic (23-40%), oleic (20-53%) and palmitoleic (11-27%). In contrast to the pulp oils, seed oils had higher amount of polyunsaturated fatty acids (PUFAs) (65-72%). The fatty acid compositions of TAGs were very close to the compositions of corresponding seed and pulp oils. The major fatty acids in PLs of berry pulp/peel oils were oleic (20-40%), palmitic (17-27%), palmitoleic (10-22%) and linoleic (10%-20%) acids, whereas in seeds PLs, PUFAs prevailed. Comparing with the other lipid fractions the SEs had the highest contents of saturated fatty acids (SFAs). The fatty acid profiles of the FFA fractions were relatively similar to those of TAGs.ConclusionsAll parts of the analyzed sea buckthorn berry cultivars (ssp. carpatica) exhibited higher oil content then the other European or Asiatic sea buckthorn subspecies. Moreover, the pulp/peel oils of ssp. carpatica were found to contain high levels of oleic acid and slightly lower amounts of linoleic and α-linolenic acids. The studied cultivars of sea buckthorn from Romania have proven to be potential sources of valuable oils.

NMR metabolomics of berry quality in sea buckthorn (Hippophae L.)

Sea buckthorn (Hippophae L.) is a woody perennial shrub or small tree whose berries are rich in bioactive compounds with powerful nutritional and medicinal properties. Untargeted 1H nuclear magnetic resonance (NMR) spectroscopy and principal component analysis (PCA) were used to characterize the metabolic profile of berry quality. There was strong separation in the NMR signal intensity of bioactive compounds between pulp and seeds, such as amino acids [gamma-aminobutyric acid (GABA), aspartate, glutamate, theanine, and proline], organic acids (citrate, succinate, malate, acetate, quinate, and heriguard), and carbohydrates (sucrose, fructose, glucose, and melibiose). Sea buckthorn cultivars could be clearly separated into two groups using principal component analysis (PCA) based on NMR spectroscopy of bioactive compounds in the pulp and seeds. Several metabolic compounds such as sugar, organic acids, and amino acids could serve as biomarkers for prediction of berry quality and for classification of germplasm collections. This dataset provides potential information concerning the mechanisms of berry quality formation and contributes to increasing the breeding efficiency of sea buckthorn quality improvement.

Fatty Acid Composition of Developing Sea Buckthorn (Hippophae rhamnoides L.) Berry and the Transcriptome of the Mature Seed

BackgroundSea buckthorn (Hippophae rhamnoides L.) is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18∶2ω-6) and α-linolenic (18∶3ω-3) acids, and pulp oil that contains high levels of monounsaturated palmitoleic acid (16∶1ω-7). Sea buckthorn is fast gaining popularity as a source of functional food and nutraceuticals, but currently has few genomic resources; therefore, we explored the fatty acid composition of Canadian-grown cultivars (ssp. mongolica) and the sea buckthorn seed transcriptome using the 454 GS FLX sequencing technology.ResultsGC-MS profiling of fatty acids in seeds and pulp of berries indicated that the seed oil contained linoleic and α-linolenic acids at 33–36% and 30–36%, respectively, while the pulp oil contained palmitoleic acid at 32–42%. 454 sequencing of sea buckthorn cDNA collections from mature seeds yielded 500,392 sequence reads, which identified 89,141 putative unigenes represented by 37,482 contigs and 51,659 singletons. Functional annotation by Gene Ontology and computational prediction of metabolic pathways indicated that primary metabolism (protein>nucleic acid>carbohydrate>lipid) and fatty acid and lipid biosynthesis pathways were highly represented categories. Sea buckthorn sequences related to fatty acid biosynthesis genes in Arabidopsis were identified, and a subset of these was examined for transcript expression at four developing stages of the berry.ConclusionThis study provides the first comprehensive genomic resources represented by expressed sequences for sea buckthorn, and demonstrates that the seed oil of Canadian-grown sea buckthorn cultivars contains high levels of linoleic acid and α-linolenic acid in a close to 1∶1 ratio, which is beneficial for human health. These data provide the foundation for further studies on sea buckthorn oil, the enzymes involved in its biosynthesis, and the genes involved in the general hardiness of sea buckthorn against environmental conditions.

Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.).

Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.

Associations of SRAP markers with dried-shrink disease resistance in a germplasm collection of sea buckthorn (Hippophae L.)

Sea buckthorn (Hippophae L.) is a woody, outcrossing dioecious pioneer plant, being widely planted as a new berry crop with rich nutritional and medicinal compounds. This long-juvenile and long-lived woody plant can be more difficult to cultivate than other crop plants. Dried-shrink disease (DSD) is a dangerous pathogen that destroys sea buckthorn and halts commercial production. We estimated variability of sequence-related amplified polymorphism (SRAP) markers in 77 accessions of 22 sea buckthorn cultivars to seek markers associated with DSD resistance and help to identify potential breeding cultivars. Seventeen SRAP primer combinations generated 289 bands, with a mean of 17 bands per primer combination. At a Dice coefficient of 0.852, the dendrogram generated with 191 polymorphic bands clustered 73 accessions of Hippophae rhamnoides into 2 groups and 4 accessions of Hippophae salicifolia into 1 group. Eleven SRAP markers (Me1-Em3(600), Me1-Em1(680), Me2-Em1(650), Me2-Em1(950), Me3-Em6(1300), Me2-Em6(320), Me2-Em6(400), Me1-Em2(600), Me1-Em1(1200), Me1-Em1(1700), Me2-Em2(250)) were significantly correlated with DSD resistance (P < 0.001). These markers provide a viable option for breeding programs that select lineages with DSD resistance, especially when no other genetic information, such as linkage maps and quantitative trait loci, are available.