Changes in content of major phenolic compounds during leaf development of sea buckthorn (Hippophaë rhamnoides L.)

Petra Scheewe,Anders Ekholm,Anne Morgenstern,Kimmo Rumpunen

doi:10.23986/afsci.9489

Abstract

Content of total phenolic compounds and antioxidant capacity (FRAP) were investigated in the leaves of three sea buckthorn (Hippophaë rhamnoides L.) cultivars: ‘Otradnaja’, ‘Gibrid Pertjika’ and ‘Ljubitelskaja’, at different dates. In addition, major phenolic compounds (catechin, kaempferol, quercetin, epigallocatechin, kaempferol-3-O-glucoside, quercetin-3-O-galactoside, isorhamnetin-3-O-glucoside, rutin, gallic acid, procyanidin monomer glycoside, procyanidin dimer aglycone and hydrolyzable tannins I–III) were determined in ‘Ljubitelskaja’. Antioxidant capacity and the content of total phenolic compounds fluctuated during April, May and June, and then increased until the end of July, when the highest values were observed. Total phenolic compounds were strongly correlated with FRAP. Levels were generally higher in ‘Ljubitelskaja’ than in ‘Otradnaja’ and ‘Gibrid Pertjika’. In ‘Ljubitelskaja’, hydrolyzable tannins I–III occurred in higher amounts than did any of the other studied phenolic compounds. The developmental stage of the leaves (harvesting date) had a strong influence on content of phenolic compounds and should be carefully considered when harvesting sea buckthorn leaves for different purposes.

Highlights

Sea buckthorn (Hippophaë L.) belongs to the family Elaeagnaceae
In this study we investigate the influence of development stage of sea buckthorn leaves of H. rhamnoides hybrid cultivars on the total antioxidant capacity, total phenolic compounds, and on the content of major single phenolic compounds
Leaf dry matter content varied during the sampling period for all three cultivars, with comparatively low values between the end of May and the beginning of July

Summary

Introduction

Sea buckthorn (Hippophaë L.) belongs to the family Elaeagnaceae. The most widely distributed species, H. rhamnoides, occurs in Central and Northern regions of Europe and Asia, and is divided into eight subspecies (Swenson and Bartish 2003). About 90% of the genetic resources are located in China, but northern India, Russia and Mongolia are rich in sea buckthorn (Singh 2003, Erkkola and Yang 2003, Ruan et al 2013). Sea buckthorn is often used as a pioneer plant to counteract soil erosion and to improve soil fertility through its N2 fixation capacity (Singh 2003, Qinxiao and Hongyan 2003). These features, together with the valuable fruits, make sea buckthorn an interesting multipurpose crop for sustainable agriculture

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Conclusion