Schiff Base HL Research Articles

Structure, Attributive and Bacterial Effectiveness Studies for Complexes, Co(II), Hg(II), Ni(II), Cu(II) and Mn(II) with Ligand kind (N2O2)

Binuclear copper, nickel, cobalt, manganese and mercury complexes of the Schiff base H2L (C40H28N2O4) obtained by condensation of 2-benzoyl benzoic acid with benzidine. The Schiff base and their complexes have been subjected to[ FT-IR, elemental analysis ,UV-Vis, 1H and 13C NMR ] spectral studies, molar conductivity, magnetic moment and HPLC measurements. All the complexes showed tetrahedral geometries with the general structure [M2(L)2]. Generality of the synthesized components offer antibacterial efficiency to (Staphylococcu saureus), (Escherichia coli),(Bacillus subtilis) and(Pseudomonas aeruginosa).

Pharmacological, electrochemical and drug–DNA interaction aspects of tridentate Schiff bases and their triphenylphosphine nickel(II) complexes

The complexes N-(2-oxidophenyl) salicylideneiminatotriphenylphosphine nickel(II) (Ni(L1)PPh3) and N-(2-sulfidophenyl)salicylideneiminatotriphenylphosphine nickel(II) (Ni(L2)PPh3) have been synthesized from Schiff base ligands N-(2-oxidophenyl)salicylideneiminate)(H2L1), N-(2-sulfidophenyl)salicylideneiminate)(H2L2) and bistriphenylphosphine nickel(II) chloride. The synthons were characterized by elemental analysis, spectroscopic (FT-IR, 1H, 13C and &31P NMR) methods and electro-analytical techniques. Single crystal X-ray diffraction analysis of complex Ni(L1)PPh3 revealed its square planar geometry where nickel being coordinated through tridentate Schiff base H2L1 and triphenylphosphine ligands. The pharmacological activities (brine shrimp cytotoxicity, antitumor, DPPH, DNA damage, antibacterial & antifungal) of the Schiff bases (H2L1 & H2L2) and their nickel complexes (Ni(L1)PPh3 & Ni(L2)PPh3) were investigated along with drug–DNA interaction studies. Results of brine shrimp lethality and antitumor assay showed highly significant activities which correlate the cytotoxic effect that can be utilized as anticancerous agents. In DPPH assay two compounds (H2L1 & Ni(L1)PPh3) showed significant activity and the remaining two compounds (H2L2 & Ni(L2)PPh3) showed protective behaviour in DNA damage assay. Antifungal assay exhibited varying degree of activities with MIC value ranging from 50 to 200µg/ml. Additionally, Ni(L2)PPh3 was found to be broad spectrum antibacterial agent by showing antibacterial activity against all tested bacterial strains. Schiff bases and their nickel complexes were found electroactive in positive and negative potential ranges respectively, while having single electron transfer process. The compounds were found highly interactive towards DNA molecules with a binding strength of about 104M−1 thus indicating their biological worth.

Synthesis, spectral and antibacterial activity of Co(II), Ni(II) and Zn(II) complexes with 2‐hydroxy‐benzoic acid(3,4‐dihydro‐2H‐naphthalen‐1‐ylidene)‐hydrazide

A bioactive Schiff base HL i.e. 2‐hydroxy‐benzoic acid(3,4‐dihydro‐2H‐naphthalen‐1‐ylidene)‐hydrazide was synthesized by reacting equimolar amount of salicylic acid hydrazide and 1‐tetralone. Co(II), Ni(II) and Zn(II) complexes of ligand HL was synthesized in 1:1 and 1:2 molar ratio of metal to ligand. The structure of the synthesized ligand and metal complexes was established by elemental analysis, molar conductance, magnetic susceptibility measurements, electronic, IR and EPR spectral techniques. For determining the thermal stability the TGA has been done. In DFT studies the geometries of Schiff bases and metal complexes were fully optimized with respect to the energy using the 6–31 + g(d,p) basis set. Spectral data reveal that ligand behave uninegative tridentate in ML complexes and uninegative bidentate in ML2 complexes. On the basis of characterization octahedral geometry has been assigned for Co(II) and Ni(II) complexes, while tetrahedral for Zn(II) complexes. Antibacterial activity of the synthesized compounds were evaluated against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Xanthomonas campestris and Pseudomonas aeruginosa and the results revealed that metal complexes show enhanced activity in comparison to free ligand.

Crystal Structure, Spectral Characterization and Biologically Studies of Mononuclear Transition Metal Complexes Derived from New N2O2 Type Ligand

The new mononuclear metal complexes viz Mn(III), Co(II), Ni(II) and Cu(II) have been synthesized by using tetradentate N2O2 donor symmetric schiff base 6,6’-((1E,1’E)-(1,2-phenylenebis(azanylylidene))bis(methanylylidene)) bis(5-isopropyl-2-methylphenol) HL and employing with corresponding metal chloride or acetate salts. After successful synthesis of compounds were thoroughly characterized by Elemental analysis, FT-IR, Uv-visible, NMR spectroscopy, LC-MS spectrometry, SEM analysis, Magnetic susceptibility measurement, Molar conductance, ESR spectroscopy. X-ray single crystal structure of HL schiff base has been determined. The synthesized compounds have been screened for their antimicrobial and antioxidant activities, which show significant results.

Synthesis, structure characterization and biological activity of selected metal complexes of sulfonamide Schiff base as a primary ligand and some mixed ligand complexes with glycine as a secondary ligand

The current work reports synthesis of metal complexes and mixed ligand complexes of a novel sulfonamide Schiff base ligand (HL) resulted from the condensation of sulfametrole [N′-(4-methoxy-1,2,5-thiadiazol-3-yl]sulfanilamide and acetyl-acetone as a primary ligand and glycine as a secondary ligand. The metal complexes and mixed ligand complexes of HL Schiff base ligand were synthesized and characterized using different physicochemical studies as elemental analyses, mass spectra, conductivity measurement, IR spectra, 1H NMR spectra, UV–vis Spectra, solid reflectance, magnetic susceptibility, thermal analyses (TGA and DTA) and their microbial and anticancer activities. The spectroscopic data of the complexes suggest their 1:2(L1:M) complex structures and 1:2:2(L1:L2:M) mixed ligand complex structures, where L1 = HL and L2 = glycine. Also, the spectroscopic studies suggested the octahedral structure for all complexes. The synthesized Schiff base, its metal and mixed ligand complexes were screened for their bacterial, antifungal and anticancer activity. The activity data show that the metal complexes and mixed ligand complexes exhibited promising microbial and anticancer activities than their parent HL Schiff base ligand, also the data show that the mixed ligand complexes more effective than the metal complexes.

Synthesis, characterization, density functional study and antimicrobial evaluation of a series of bischelated complexes with a dithiocarbazate Schiff base ligand

A nitrogen-sulfur Schiff base HL (1) derived from S-hexyldithiocarbazate and 4-methylbenzaldehyde has been reacted with different divalent metal ions in 2:1 molar ratio, producing neutral complexes (2–7) of general formula MIIL2 (where M=Ni, Cu, Zn, Cd, Pd and Pb). All compounds were characterized using established physico-chemical and spectroscopic methods. The single crystal structures of CuII and ZnII complexes are compared and discussed with those of NiII and PdII already reported by us, underlining the geometrical variations occurring in the HL ligand upon coordination. The metal complexes, as revealed by the X-ray diffraction analyses, show a square planar or tetrahedral coordination geometry, and in the former case either a cisoid or transoid configuration of chelating ligands. Density functional theory (DFT) and time-dependent density functional theory (TD-DFT) calculations have been performed on the isolated cis/trans complexes of Ni and Pd complexes in order to evaluate the stability of the isomer isolated in solid state. The thermodynamic parameters for trans to cis isomerization of NiL2 complex [ΔH=−29.12kJ/mol and ΔG=−43.97kJ/mol] indicated that the trans isomer (observed in solid state) is more stable than the cis one. On the other hand, relative enthalpy [ΔH=−4.37kJ/mol] and Gibbs free energy [ΔG=−5.50kJ/mol] of PdL2 complex disclosed a small difference between the energies of the two isomers. Experimental UV–vis and TD-DFT calculation confirmed that these complexes have distinctive LMCT bands with a broad shoulder at 400–550nm. With the purpose of providing insight into the properties and behavior of the complexes in solution, photoluminescence and electrochemical experiments have been also performed. Finally, the anti-bacterial activity of these compounds was evaluated against three pathogenic Gram-negative organisms such as Escherichia coli, Salmonella typhi and Shigella flexneri, but only the free ligand 1 showed anti-bacterial property against all three tested organisms.

Synthesis, of some transition metal (ΙΙ/ΙΙΙ)-Schiff base complexes and copper, zinc nanoparticle size bearing sulfonamide fragment : New drugs for antimicrobial and anticancer agents.

A simple approach to the synthesis metal complexes and copper, zinc nano particles size (CuNPs, ZnNPs) of novel Schiff base of sulfonamide ligand (HL) resulted from the condensation of sulfametrole [N'-(4-methoxy-1,2,5-thiadiazol-3-yl]sulfanilamide and 1-(2-Furanyl) ethanon. The metal complexes and copper, zinc nano particles size (CuNPs, ZnNPs) of HL ligand were synthesized and characterized using different physico-chemical studies as elemental analyses, mass spectra, conductivity measurement, UV-vis Spectra, solid reflectance, IR spectra,,1H NMR spectra, magnetic susceptibility, thermal analyses (TGA and DTA), (TEM) Transmission Electron Microscope and their microbial and anticancer activities . The spectroscopic data of the complexes suggest their 1:2(L:M) complex structures. Also the spectroscopic studies suggested the octahedral structure for all complexes. The size and morphology investigation of copper and zinc nano particles (CuNPs, ZnNPs) with (HL) ligand by TEM images indicate that the copper and zinc nano (CuNPs, ZnNPs) show spherical shape with core shell of HL ligand. The synthesized Schiff base and its metal complexes, CuNPs, ZnNPs were screened for their bacterial, antifungal and anticancer activity. The activity data show that the metal complexes, CuNPs and ZnNPs to be more potent than the parent HL Schiff base ligand. Also the data show that the CuNPs and ZnNPs more potent than the metal complexes and than HL free ligand .

Antiferro- to ferromagnetic crossover in diphenoxido bridged NiII2MnII complexes derived from N2O2 donor Schiff base ligands

Three new trinuclear NiII–MnII complexes have been synthesized using three different “metalloligands” [NiL1], [NiL2] and [NiL3] derived from the Schiff bases H2L1=N,N′-bis(salicylidene)-1,3-pentanediamine, H2L2=N,N′-bis(salicylidene)-1,3-propanediamine and H2L3=N,N′-bis(salicylidene)-1,3,-diaminopropan-2-ol), respectively. In all three complexes, [(NiL1)2Mn(OOCPh)2(H2O)2]·CH3OH (1), [(NiL2)2Mn(OOCPh)2(CH3OH)2]·CH3OH (2) and [(NiL3)2Mn(OOCPh)2(H2O)2]·CH3OH (3), in addition to the double phenoxido bridge, the two terminal NiII atoms are linked to the central MnII by means of a syn–syn bridging benzoate, giving rise to a linear structure. Complex 1 with Ni–O–Mn angle of 97.35° exhibits antiferromagnetic interactions (JNi–Mn=−0.60cm−1) whereas ferromagnetic exchange is observed in 2 and 3 (JNi–Mn=+2.00 and +1.10cm−1 respectively) having Ni–O–Mn angle 97.34° (in 2), 97.27° (in 3). Theoretical calculations have been performed in order to understand the effect of structural parameters that can tune the magnetic properties of this type of complexes such as small differences in the Ni–O–Mn angle and/or slight variations in intermolecular contacts within the crystal.

Manganese(II) coordination polymer having pyrazine and μ-phenolato bridging: Structure, magnetism and biological studies

The manganese(II) coordination polymer, {[Mn(L)(pyz)]ClO4·2(H2O)}n. (1) has been synthesized from a mixture of Mn(ClO4)2·6H2O, Schiff base HL (derived from the condensation of 4-aminoantipyrine and salicylaldehyde) and pyrazine (pyz). Its molecular structure was determined by single crystal X-ray diffraction, which reveals that the polymeric structure consists of simultaneous Mn–Pyz–Mn and Mn–O(phenoate)–Mn bridges between the metal centers. Variable temperature magnetic studies give g=2.037(4), J=−1.90(4)cm−1, ϑ=0.5K values, using a simple dinuclear Mn–Mn model, and indicates that the major exchange pathway is via the two phenoxide bridges and the pyrazine has little influence. The coordination polymer also shows enhanced antibacterial activity compared with the standard antibiotic levofloxacin.

Crystal structures and mesomorphic properties of Schiff base homologs and derivatives, and magnetic properties of their dimeric and dinuclear copper(II) complexes

ABSTRACTThree N2O4 Schiff. base homologs, H2L1 (n = 8), H2L3 ((n = 9), and H2L4 (n = 10) were obtained from the reactions of 2-HOC6H4CHO with H2N(CH2)8-10NH2, while a derivative of H2L1, namely, H2L2, was obtained from the reaction of 3,5-X2-2-HOC6H2CHO (X = tert-butyl) with H2N(CH2)8NH2. The Schiff bases H2L2 (triclinic; P-1), H2L3 (monoclinic; C2/c), and H2L4 (monoclinic; P21/c) were single crystals with low melting temperatures (less than 100°C). The homologs exhibited mesomorphisms, while the derivative was not mesomorphic and did not reform crystals from its melt. Copper(II) complexes of H2L1, H2L2 and H2L4 were dimeric, while that of H2L3 was dinuclear with chelating CH3COO ligand. These complexes were paramagnetic with insignificant interactions between the copper(II) atoms and have high decomposition temperatures (Tdec = 268–304°C). Their melting temperatures (Tmelt = 138.9–190.2°C) were higher than the corresponding Schiff bases, but they were not mesomorphic.

N-HO and N-HCl supported 1D chains of heterobimetallic CuII/NiII-SnIV cocrystals.

The Schiff base H2L1 [N,N'-ethylenebis(3-methoxysalicylaldimine)] or H2L2 [N,N'-ethylenebis(3-ethoxysalicylaldimine)] was reacted with MCl2·xH2O and SnCl4·5H2O to afford the supramolecular heterobimetallic systems (H2ED)2+·2[ML]·[SnCl6]2- [M = Cu, L = L1 (1), L = L2 (2); M = Ni, L = L1 (3), L = L2 (4); ED = 1,2-ethylenediamine], whose structures were established by single crystal X-ray analyses. Each structure includes different entities, viz. a mononuclear [CuL]/[NiL] neutral complex (coformer), a hexachlorostannate dianion [SnCl6]2-, a 1,2-ethylenediammonium dication (H2ED2+) and, only in 2 and 4, a methanol molecule. Based on the work of Grothe et al. (Cryst. Growth Des., 2016, 16, 3237-3243), compounds 1 and 3 are cocrystal salts, 2 and 4 are cocrystal salt solvates. The ionic pairs (H2ED)2+·[SnCl6]2- in 1-4 are encapsulated by the Cu- or Ni-complexes, and stabilized by N-HO and one N-HCl bond interactions leading to infinite 1D chains. The antimicrobial studies of 1-4 against yeasts (C. albicans and S. cerevisiae) and Gram-positive (S. aureus and E. faecalis) and -negative bacteria (P. aeruginosa and E. coli) indicate that the Ni2Sn systems (3 and 4) are more active than the analogous Cu2Sn ones (1 and 2).

A tetranuclear holmium compound exhibiting single molecule magnet behavior

The synthesis, structure, and magnetic properties of a new tetranuclear holmium cluster [HoIII4(μ3-OH)2(L)4(piv)2(DMF)2]·2DMF (1) is reported, where the Schiff base H2L ligand is 2-hydroxy-3-methoxy-phenylsalicylaldimine and piv is pivalate. The cluster has a planar “butterfly” Ho4 core, which is bridged by two μ3-hydroxide and six phenoxide oxygen atoms. Magnetically, compound 1 displays field-induced slow relaxation of magnetization. Fitting the dynamic magnetic data to the Arrhenius law gives energy barrier ΔE/kB=21.49K and τ0=6.97×10−7s.

Tetra(μ3-hydroxo) bridged copper(II) tetranuclear cubane complexes: synthesis, crystal structure, and DNA binding studies

The tridentate Schiff base H2L was synthesized by the condensation of equimolar amount of 1-amino-2-propanol and salicylaldehyde. The reaction of H2L with an equimolar amount of Cu(CH3COO)2·H2O in methanol leads to the formation of the tetranuclear Cu4L4, 1. However, reaction of equimolar amount of H2L, copper(II) acetate, and 2,4,6-trimethylaniline in methanol forms a mixture of products which includes a discrete mononuclear complex Cu(L′)2, 2m (where HL′ is a bidentate ligand), in addition to the tetranuclear Cu4L4 species, 2c. In both tetranuclear cubane species, the tridentate H2L is both a chelating and a bridging ligand, after deprotonation of the enolic and the phenolic OH. The copper(II) centers are five-coordinate with a [N, O4] donor set from the ligands. The coordination geometry about each copper is distorted square pyramidal with one nitrogen and two oxygen from one ligand and two oxygen from adjacent ligands in the next unit of the cubane. In mononuclear 2m, the ligand is bidentate and the coordination geometry around copper(II) is square planar. The absorption spectra strongly suggest that tetranuclear 1 interacts with CT-DNA.

Synthesis, spectroscopic, coordination and biological activities of some transition metal complexes containing ONO tridentate Schiff base ligand

The main target of this paper is to get an interesting data for the preparation and characterizations of metal oxide (MO) nanoparticles using H2L Schiff base complexes as precursors through the thermal decomposition procedure. Five Schiff base complexes of Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) metal ions were synthesized from 2-[(2-hydroxy-naphthalen-1-ylmethylene)-amino]-benzoic acid new adduct (H2L). Theses complexes were characterized using infrared, electronic, mass and 1H NMR spectroscopic techniques. The elemental analysis data was confirmed that the stoichiometry of (metal:H2L) is 1:1 molar ratio. The molar conductance indicates that all of complexes are non electrolytic. The general chemical formulas of these complexes is [M(L)(NH3)]·nH2O. All complexes are tetrahedral geometry. The thermal decomposition behavior of H2L hydrated and anhydrous complexes has been discussed using thermogravimetric analysis (TG/DTG) and differential thermal analyses (DTA) under nitrogen atmosphere. The crystalline phases of the reaction products were checked using X-ray diffractometer (XRD) and scanning electron microscopy (SEM).

Syntheses, characterizations, X-ray crystal structures, and antibacterial activities of Co(II), Ni(II), and Zn(II) complexes of the Schiff base derived from 5-nitro-2-hydroxybenzaldehyde and benzylamine

Three new complexes, [CoIIL2(bzln)2] (1), [NiIIL2(DMF)2] (2), and [ZnIIL2] (3), with the bidentate (NO) Schiff base HL = 2-((E)-(benzylimino)methyl)-4-nitrophenol have been synthesized and characterized. [CoIIL2(bzln)2] (1) was initially formed from the reaction of H2L1 (N,N′-bis(5-nitrobenzaldehyde)-2-aminobenzylamine = H2L1), with CoII(CH3COO)2·4H2O in the presence of benzylamine. The X-ray crystal structure of 1 revealed that HL was formed from the hydrolysis of H2L1 and the recondensation of benzylamine with 2-hydroxy-5-nitrobenzaldehyde produced in H2L1 hydrolysis. The Schiff base HL was then prepared by direct reaction of 2-hydroxy-5-nitrobenzaldehyde and benzylamine. Complexes 1–3 were also directly synthesized and their crystal and molecular structures determined by X-ray crystallography. The ligand and its metal complexes were also screened for in vitro antibacterial activity.

Sulfonated Schiff base copper(ii) complexes as efficient and selective catalysts in alcohol oxidation: syntheses and crystal structures

Alcohol oxidation properties of four copper complexes (2–5) derived from the sulfonated Schiff base H2L·3H2O (1) are described. A quantitative conversion of 1-phenylethanol was obtained with4within 20 min of low power MW irradiation.

Synthesis and characterization of manganese(II), cobalt(II), nickel(II), copper(II), and zinc(II) mixed ligand complexes with [(1-phenyl-3- methyl-5- hydroxopyrazol-4-yl) methylimino] 2′, 3′ dimethylaniline and 2- hydroxy-1-naphthaldehyde

Mixed ligand complexes of the type [MLL ' ] where M = Mn (II), Co (II), Ni (II), Cu (II), Zn (II) HL = [(1-phenyl-3methyl-5-hydroxopyrazol-4-yl)phenylimino]2,3-dimethylaniline; (HPMPZP)dma. HL ' = 2-hydroxy-1-naphaldehyde; (HNA), have been synthesized and characterized on the basis of elemental analysis, conductivity measurements, magnetic, electronic and infra red spectral studies. The complexes confirm to 1:1:1 stoichiometry and are non electrolytes. The schiff base HL act as a monovalent bidentate ligand co-ordinating through azomethine nitrogen and phenolic oxygen. On the basis of electronic spectra, IR spectra and magnetic moment measurements; six coordinated octahedral structures have been proposed for the complexes. Thermal studies revealed the presence of two co-ordinated water molecules. The shiff base and mixed ligand complexes have been tested for their antibacterial activity against the Escherichia coli, Bacillus substilis, staphylococcus aureus.

Synthesis, characterization and bio-activity of nickel(II) and copper(II) complexes of a bidentate NS Schiff base of S-benzyl dithiocarbazate

The reaction of S-benzyldithiocarbazate (SBDTC) with 3-hydroxyacetophenone afforded a bidentate NS Schiff base HL (HL=benzyl-3-(3-hydroxyphenyl methyl ethylene)hydrazine carbodithioate) which on further reaction with nickel(II) and copper(II) salt yielded bis-chelated inner complexes, NiL2 and CuL2, respectively with deprotonated L ligand. The ligand and its complexes were characterized by physico-chemical techniques, viz., molar conductance, magnetic susceptibility measurement, IR, NMR, UV–Vis spectroscopy and mass spectroscopic techniques. The crystal structure of all the compounds was also characterized by single crystal X-ray crystallography. The ligand exists in its thione tautomeric form both in solution and in the solid state. In the complexes the two deprotonated ligands chelate the metal through the azomethine nitrogen and the thione sulfur atom with cis arrangement generating a tetrahedrally distorted square planar geometry around the metal center. Only complex CuL2 showed moderate analgesic activity at 60min compared to Diclofenac sodium, while all the test compounds exhibited good anti-inflammatory activity as compared to standard drug Indomethacin.

Mononuclear Complexes Based on Pyrimidine Ring Azo Schiff‐Base Ligand: Synthesis, Characterization, Antioxidant, Antibacterial, and Thermal Investigations

AbstractSix transition metal(II) complexes with the heterocyclic ligand HL (1), [CuL2]·H2O (2), [NiL2]·3H2O (3), [CoL2]·3H2O (4), [MnL2]·3H2O (5), [ZnL2]·2H2O (6), [PdLOAc]·H2O (7) [HL = 5‐benzoyl‐1‐((E)‐(2‐hydroxy‐5‐((E)‐phenyldiazenyl)benzylidene)amino)‐4‐phenylpyrimidin‐2(1H)‐one] were synthesized. The features of the azo Schiff bases were assigned from microanalytical, spectroscopic (IR, UV/Vis., 1H‐ and 13C NMR, API‐ES mass), magnetic, and molar conductivity measurements at room temperature as well as thermal analysis. The electronic absorption spectroscopy and magnetic susceptibility measurements of the complexes indicate square pyramidal arrangement for PdII and octahedral environment for all the other complexes. The azo Schiff base HL acts as a monobasic tridentate ligand, which commonly coordinates through the oxygen atoms of the phenol OH and the pyrimidine one group, and the nitrogen atom of the azomethine group. The thermal behaviors of the ligand and its metal complexes were studied using thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The metal complexes proved to be more thermally stable than the ligand; they decomposed at 10–30 °C higher temperatures. Antioxidant properties of the ligand and its metal complexes (DPPH free radical scavenging, ferrous chelating and reducing power activities) were tested. Antimicrobial activities were studied with gram‐positive bacteria, which included Bacillus subtilis and Staphylococcus aureus, whereas Escherichia coli and Pseudomonas aeruginosa represented gram negative bacteria.

Effects of copper ions on DNA binding and cytotoxic activity of a chiral salicylidene Schiff base

A chiral Schiff base HL N-(5-bromo-salicylaldehyde)dehydroabietylamine (1) and its chiral dinuclear copper complex [Cu2L4]·4DMF (2) have been synthesized and fully characterized. The interactions of 1 and 2 with salmon sperm DNA have been investigated by viscosity measurements, UV, fluorescence and circular dichroism (CD) spectroscopic techniques. Absorption spectral (Kb=3.30×105M−1 (1), 6.63×105M−1(2)), emission spectral (Ksv=7.58×103M−1 (1), 1.52×104M−1 (2)), and viscosity measurements reveal that 1 and 2 interact with DNA through intercalation and 2 exhibits a higher DNA binding ability. In addition, CD study indicates 2 cause a more evident perturbation on the base stacking and helicity of B-DNA upon binding to it. In fluorimetric studies, the enthalpy (ΔH>0) and entropy (ΔS>0) changes of the reactions between the compounds with DNA demonstrate hydrophobic interactions. 1 and 2 were also screened for their cytotoxic ability and 2 demonstrates higher growth inhibition of the selected cancer cells at concentration of 50μM, this result is identical with their DNA binding ability order. All the experimental results show that the involvement of Cu (II) centers has some interesting effect on DNA binding ability and cytotoxicity of the chiral Schiff base.