The volvocalean microalga Haematococcus pluvialis is used as a sourceof the ketocarotenoid astaxanthin for applications in aquaculture and thepharmaceutical and cosmetic industries. This green alga accumulatesastaxanthin, mostly esterified, canthaxanthin and echinenone in lipid vesiclesoutside the chloroplast. This accumulation process normally but notexclusively accompanies formation of the resting state in the developmentalcycle. With regard to increased bioavailability of the accumulated secondarycarotenoids, the fragility of the extracellular matrix makes the flagellatedstate of H. pluvialis an interesting alternative to the thick-walledaplanospore state. A two-step batch cultivation scheme was developed thatleads to accumulation of secondary carotenoids in flagellated cells of H. pluvialis (strain 192.80, Gottingen, Germany). Germination ofgreen aplanospores during the first step of cultivation proceeded optimallyunder 30 μmol photon m-2 s-1 of whitefluorescent light at 20 °C. For optimal induction and enhancementof carotenoid biosynthesis, the flagellated cells formed were then exposedto a decreased level of nitrate (0.4 mM KNO3) and to enhancedirradiance (150 μmol photon m-2 s-1). Under theseconditions, which still permitted cell division and chlorophyll synthesisduring the first two days of exposure, carotenoid accumulation in theflagellated cells reached 2° of dry mass at the fourth day of exposure. Asa mixotrophic carbon source, addition of acetate at a concentration nothigher than 10 mM increased carotenoid synthesis only slightly whereaspartial or complete phosphate deficiency or salt stress (40 mM NaCl) didnot.