SC-CO2 Extraction Research Articles

Extraction and Characterization of Fatty Acids from the Leaves and Stems of Clinacanthus nutans Using Supercritical Carbon Dioxide and Soxhlet Method

Background: The composition and bioactivity of natural plant extract strongly depend on the extraction technique employed. Clinacanthus nutans Lindau (C. nutans) is a well-known medicinal plant in South-East Asia that has been traditionally used for the treatment of various diseases. Several conventional methods have been using for the extraction of bioactive compounds from C. nutans. However, extraction of fatty acids using supercritical carbon dioxide was not reported yet from this medicinal herbs. Objective: The main objective of the study is to examine the potential of supercritical carbon dioxide (scCO2) extraction of fatty acids from leaves and stems of C. nutans. Method: Fatty acid compositions were determined from leaves and stems of C. nutans oil extracted by scCO2 (temperature 45-65 °C, pressure 25-35 MPa) and compared to the results of Soxhlet extraction. Results: Supercritical CO2 extraction shows the highest oil recovery in both leaves (3.7%) and stems (1.6%) at pressure 35 MPa, temperature 65°C and 2 ml/min flow rate, which was closer to the yield of Soxhlet. The scCO2 yields presented a higher percentage of polyunsaturated fatty acids (PUFA), especially linoleic acid (C18:2n-6). Palmitic acid ranging from 42%- 47% in leaves and stems of C. nutans was found dominant saturated fatty acids (SFA) in both scCO2 and Soxhlet method. Conclusion: The current results indicate that leaves and stems of C. nutans could be a potential source of fatty acids, especially biologically active compounds.

Cytoprotective, antioxidant, and anti-migratory activity of Pistacia lentiscus L. supercritical carbon dioxide extract on primary human endothelial cells

Green chemistry is a useful tool for producing valuable chemicals from biomass. However, extracted compounds need to be tested for safety and efficacy before their use in humans. Here we investigate the chemical composition and biological effects of a leaves Pistacia lentiscus L. supercritical carbon dioxide (SCCO2) extract. Terpenes represented the main extract fraction, with Germacrene D (11.18%), delta-cadinene (10.54%), and alpha-pinene (8.7%) the most abundant molecules. Challenged with endothelial cells (ECs), increasing extract concentrations failed to affect cell proliferation or promote cell toxicity. ROS assessment in unstressed and H2O2-treated ECs revealed an extract dose-dependent antioxidant activity. Exposition of H2O2-treated ECs to increasing extract concentrations dose-dependently counteracted H2O2-induced cell impairments. The extract significantly counteracted fetal calf serum-induced ECs migration. For the first time, we report that a SCCO2 extract obtained from PL leaves is safe on ECs and may be a useful source of valuable compounds with vasculoprotective properties.

Extraction of Municipal Sewage Sludge Lipids Using Supercritical CO2 for Biodiesel Production: Mathematical and Kinetics Modeling

Demand for determining renewable lipids feedstock for the production of biodiesel is increasing with the rapid depletion of petroleum diesel. The present study was conducted to assess the feasibility of utilizing municipal sewage sludge (MSS) as a potential lipids feedstock for biodiesel production. The lipids’ extraction and separation from MSS were conducted using supercritical CO2 (scCO2) with varying treatment time (15–120 min), temperature (30–80°C), pressure (10–50 MPa), and addition of cosolvents (1–10 wt.%). The modified Gompertz equation and Arrhenius equation were employed to evaluate lipids’ extraction and kinetics behavior from municipal sewage sludge using scCO2. About 27% of lipids were extracted from MSS with scCO2 at a temperature of 60°C, pressure of 30 MPa, treatment time of 60 min, and 5 wt.% of ethanol (EtOH) as cosolvent. The modified Gompertz equation was adequately fitted with experimental data of the lipids’ extraction from MSS using scCO2. The kinetics properties analyses revealed that the scCO2 extraction technology was highly dependent on pressure than the temperature for the extraction of the lipids from MSS. The physicochemical characteristics and fatty acid contents of the scCO2 extracted lipids from MSS and sewage sludge biodiesel were determined using a variety of analytical techniques. The physicochemical properties of the sewage sludge biodiesel were compared with the international standard specifications of biodiesel, such as the American Society for Testing and Materials specifications for diesel fuel (ASTM D6751) and European Standard (EN 14214) specifications.

Inedible oil feedstocks for biodiesel production: A review of production technologies and physicochemical properties

Biodiesel emits lesser harmful pollutant emissions than renewable and biodegradable ones compared diesel. Research confirms that edible products and crops are the major sources of biofuel production. Excessive usage of these crops leads to higher production costs, economic imbalance, and depletion of food supply. Biofuel production from inedible sources shall lower the drawbacks of edible products and crops. Inedible feedstocks are the sustainable source of biofuel production as they are mostly grown on waste/abandoned land, produce similar or higher yields than edible feedstocks, and are fairly cost-effective. Hence this present work reviews the challenges and possibilities of employing inedible oil and products as a potential feedstock for biofuel production. Salient features of inedible oil such as production technologies, cost and benefits, fatty acid and physicochemical properties and oil extraction technology are reviewed from the latest literature. The outcome of this study suggests that there is a huge prospect of utilizing inedible oil as a reliable feedstock for biofuel generation. Among various production processes, scCO2 extraction technology proved to reduce inedible oil's moisture by 70% and FFA content by 62%, with a higher conversion rate of about 97%, as methanol in supercritical conditions has lesser interaction with the FFA of inedible oil. Inedible feedstocks are effective, non-toxic and safe in biofuel production. However, there exists a challenge in restricting its development in large-scale commercialization.

Hass and Fuerte avocado (Persea americana sp.) oils extracted by supercritical carbon dioxide: Bioactive compounds, fatty acid content, antioxidant capacity and oxidative stability

Avocado oils (AO) from Hass and Fuerte varieties were extracted by supercritical CO2 (scCO2) at 40 and 80 °C (400 bar). The yields of the extraction ranged from 36 % to 38 % and AO extraction using scCO2 showed a good fit to the logistic model. Physicochemical, bioactive compounds, fatty acid composition, antioxidant capacity and oxidative stability of the oil were influenced by scCO2 extraction. Compared to commercial product extracted by cold pressing, the AO extracted with scCO2 showed a lower total phenolic content, except for that extracted from the Fuerte variety at 80 °C, as well as higher total carotenoid and chlorophyll contents, unsaturated/saturate fatty acid ratio and antioxidant capacity in that extracted at 80 °C. However, initiation and propagation kinetic parameters, estimated at the first time using accelerated Oxitest system, showed that AO obtained by scCO2 is more susceptible to lipid oxidation.

Selective Supercritical CO2 Extraction and Biocatalytic Valorization of Cucurbita pepo L. Industrial Residuals.

The valorization of biomass residuals constitutes a key aspect of circular economy and thus a major challenge for the scientific community. Among industrial wastes, plant residuals could represent an attractive source of bioactive compounds. In this context, a residue from the industrial extraction of Cucurbita pepo L. seeds, whose oil is commercialized for the treatment of genito-urinary tract pathologies, has been selected. Supercritical CO2 technology has been employed as a highly selective “green” methodology allowing the recovery of compounds without chemical degradation and limited operational costs. Free fatty acids have been collected in mild conditions while an enrichment in sterols has been selectively obtained from sc-CO2 extracts by appropriate modulation of process parameters (supercritical fluid pressure and temperature), hence demonstrating the feasibility of the technique to target added-value compounds in a selective way. Obtained fatty acids were thus converted into the corresponding ethanol carboxamide derivatives by lipase-mediated biocatalyzed reactions, while the hydroxylated derivatives of unsaturated fatty acids were obtained by stereoselective hydration reaction under reductive conditions in the presence of a selected FADH2-dependent oleate hydratase.

Supercritical carbon dioxide extraction of squalene rich cod liver oil: Optimization, characterization and functional properties

Cod liver oil (CLO) was extracted using conventional hexane and pressing methods or ecofriendly supercritical carbon dioxide (SC-CO2). The extraction main parameters including temperature (A), pressure (B), and CO2 flow rate (C) were optimized using response surface methodology. The oil chemical composition, safety, thermostability, and biological properties were characterized. The optimized SC-CO2 extraction conditions were A = 49 °C, B = 29.9 MPa, and C = 4.97 mL/min. Time-dependent extraction curves revealed a 68%, 85%, and 89% CLO recovery in 5, 15, and 20 h, respectively. SC-CO2-extracted oil contained the highest squalene (≈150 µg/mL) and vitamins D and K concentrations and the lowest toxic heavy metal levels. Thermogravimetric analysis indicated that SC-CO2-extracted oil was more susceptible to thermal degradation because of its high purity. SC-CO2-extracted CLO exhibited radical scavenging and antimicrobial functions and was cytotoxic for cancer cells suggesting its potential use in the nutraceutical industry and novel functional material designs.

Application of response surface methodology (RSM) for optimization of the supercritical CO2 extract of oil from Zanthoxylum bungeanum pericarp: Yield, composition and gastric protective effect.

Supercritical carbon-dioxide (SC-CO2) is a promising two-phase technology for flavor components (volatile oil and alkylamides) extract from Zanthoxylum bungeanum pericarp. However, the gastric protective effect of SC-CO2 extract from Z. bungeanum (SZB) have not been systematically investigated. In this study, response surface methodology (RSM) was employed to optimize the yield of SZB, and the average yield of 11.07% were obtained under optimal parameters (30MPa, 43°C and time 75min). Here, limonene, linalool and hydroxy-α-sanshool were identified as the main compounds of SZB by GC-MS and UPLC-Q-Extractive Orbitrap/MS analysis. When the gastric protective effect of SZB (5, 10 and 20mg/kg, p.o.) were evaluated, significant increase in body weight and organ indexes of rat, and decreased gastric lesion were observed. Furthermore, nineteen serum metabolites were regarded as the potential biomarkers for the gastric protective effect of SZB. Collectively, this study provides a comprehensive perspective into the chemical composition analysis and gastric protective effect of Z. bungeanum SC-CO2 extract.

Extraction and encapsulation of squalene-rich cod liver oil using supercritical CO2 process for enhanced oxidative stability

We produced squalene-rich cod liver oil (CLO) and solid-lipid particles (SLP) were produced using supercritical carbon dioxide (SC-CO2) extraction and gas saturated solutions (PGSS) processes respectively. Polyethylene glycol (PEG) was used for encapsulation. The encapsulation yield and efficiency (EE) of the SLPs obtained from the oil under 25 MPa, 4:20 (SCO:PEG, w/w) condition, were 71.13 % ± 0.44 % and, EE 35.52 % ± 0.22 %, respectively. The optimum conditions included 25 MPa, 4:20 (SCO:PEG, w/w), the bulk density and tapped density of the SLPs were 0.30 ± 0.02 and, 0.33 ± 0.02 g/cm3, respectively. The corresponding Carr’s index (CI) value was the lowest at 9.09 ± 0.97 and the smallest particle size was 315.83 µm with 9-PEG, making it suitable for commercial applications. Microencapsulated CLO was characterized using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimeter (DSC). Upon evaluation, the antioxidant activity of squalene was enhanced with 9-PEG at ABTS+ 142.60 ± 5.71 mgTE/g and, DPPH 30.38 ± 1.44 mgTE/g. Encapsulated CLO (22.50 ± 0.30 meq/kg) showed improved oxidative stability than bare CLO. Therefore, encapsulation of functional oils obtained via SC-CO2 extraction using PGSS process inhibits oxidation and facilitates its commercial applications.

Supercritical CO2 Extract from Microalga Tetradesmus obliquus: The Effect of High-Pressure Pre-Treatment.

High-pressure pre-treatment followed by supercritical carbon dioxide (ScCO2) extraction (300 bar, 40 °C) was applied for the attainment of the lipophilic fraction of microalga Tetradesmus obliquus. The chemical profile of supercritical extracts of T. obliquus was analyzed by ultra-high-performance liquid chromatography–high-resolution mass spectrometry with electrospray ionization (UHPLC-ESI-HRMS). Moreover, the impact of ScCO2 on the microbiological and metal profile of the biomass was monitored. The application of the pre-treatment increased the extraction yield approximately three-fold compared to the control. In the obtained extracts (control and pre-treated extracts), the identified components belonged to triacylglyceroles, fatty acid derivatives, diacylglycerophosphocholines and diacylglycerophosphoserines, pigments, terpenes, and steroids. Triacylglycerols (65%) were the most dominant group of compounds in the control extract. The pre-treatment decreased the percentage of triacylglycerols to 2%, while the abundance of fatty acid derivatives was significantly increased (82%). In addition, the pre-treatment led to an increase in the percentages of carotenoids, terpenoids, and steroids. Furthermore, it was determined that ScCO2 extraction reduced the number of microorganisms in the biomass. Considering its microbiological and metal profiles, the biomass after ScCO2 can potentially be used as a safe and important source of organic compounds.

Pinus radiata bark sequentially processed using scCO2 and an ionic liquid catalyst yields plentiful resin acids and alkanoic acids enriched suberin

The bark of Pinus radiata is an under-utilized forest residue that is renewable, abundant and has the potential to become a source of sustainable high-value chemicals. However, the use of this bark within a biorefinery for advanced applications is hindered by its intractable characteristics: high integrity, complex composition, and high heterogeneity. Most of the bark is burnt to provide energy and heat. The bark contains a high portion of phenolic extractives, constituting a potential source of valuable compounds. It also contains the heteropolymer suberin, a source of unique building blocks for developing innovative materials with potential broad bactericidal properties. Removal of phenolic extractives and suberin from bark simplifies down-streaming pulping processing of bark’s lignocellulosic part. Herein, we describe an effective green strategy to sequentially extract the lipophilic bark constituents and suberin, exploring scCO2 (40, 50 or 60 °C / 200, 350 or 500 bar) and a biocompatible ionic liquid catalyst. The obtained scCO2 extracts had similar diversity of lipophilic compounds and predominantly contained resin acids. Further extraction of the scCO2 extracted bark yielded suberin amounts of 2.25% wt. The bark’s suberin structure shows archetypal chemical features yet has an idiosyncratic high abundance of alkanoic acids, which is not common in most sources. The findings of this opening bark biorefinery study deserve further development and complementary techno-economic analyses to secure new value chains for the bark's major lipophilic compounds consisting of resin acids and bark suberin.

Edible oils as a co-extractant for the supercritical carbon dioxide extraction of flavonoids from propolis.

Propolis is a good source for flavonoids, however, their recovery is challenging, as it is a waxy material. This study investigated edible oils virgin coconut oil (VCO), corn oil (CO), and ghee (G) as co-extractants for the supercritical carbon dioxide (scCO2) extraction of flavonoids from the propolis. The extraction of flavonoids using 20% VCO as co-extractant with scCO2 (25 g/min) for 210 min at 150 bar and 50°C was found to be the most appropriate, yielding a total flavonoid content (TFC) of 11.7 mg/g and 25% TFC recovery. At a higher temperature (60°C) and pressure (250 bar and 350 bar), the propolis became softer and compressed causing the extractions to retrograde. The extraction curves correlated to the diffusion model with 1.6% (AARD). The matrix diffusivities increased from 4.7 × 10−11 m2/s (scCO2) to 6.9 × 10−11–21.4 × 10−11 m2/s upon the addition of edible oils. Thus, edible oils could be used with scCO2 to improve the flavonoid extraction from propolis.

Supercritical fluid extraction of oil from medlar (Mespilus germanica l.) seeds

The production of SC CO2 extracts from different types of biologically active raw materials growing in different soil and climatic conditions has been studied and continues to be studied. The object of this study was the bones of a wild medlar (Mespilus germanica L.) growing in Azerbaijan. The purpose of this study was to determine the optimal technological parameters of extraction. The experimental part of the work included obtaining oil from medlar seeds by supercritical fluid extraction with carbon dioxide at the extraction plant of the company “SITEC” Switzerland at different technological parameters and determining the content of fatty acids by gas-liquid chromatography with mass-selective detection (GC-MS). Data were obtained characterizing the oil yield in a wide range of temperatures (40 …80°C) and pressures (100 …280 bar), as well as its physico-chemical parameters and fatty acid profile. It has been established that the optimal parameters for the extraction process are a pressure of 150-250 bar (preferably 200 bar), a temperature of 40°C, a time of 1 - 1.5 h. The data obtained allow us to get a more complete picture of the biotechnological potential of the local medlar as a raw material for processing by modern extraction methods.

Antioxidant, Antimicrobial and Antibiofilm Properties of Glechoma hederacea Extracts Obtained by Supercritical Fluid Extraction, Using Different Extraction Conditions

Glechoma hederacea var. longituba is a herbaceous plant from the Lamiaceae family, used in herbal medicine. In this work, we aimed to assess the total phenolic content, antioxidant, antimicrobial and antibiofilm activity of extracts obtained from G. hederacea via supercritical dioxide extraction with methanol as a co-solvent under different extraction conditions. The results showed that the activity of the obtained SC-CO2 extracts is strongly dependent on the extraction temperature. Significantly higher total polyphenol content, as well as antioxidant and antimicrobial activity towards bacteria and yeasts, was observed in the extract obtained at 40 °C, compared to extracts obtained at 50 °C and 60 °C; however, antifungal activity against filamentous fungi was not dependent on the extraction conditions. Antimicrobial activity also depended on the microorganism type. Higher sensitivity was exhibited by Gram-positive bacteria than by Gram-negative bacteria, with S. aureus and P. aeruginosa being the most sensitive species among each group. The most susceptible fungi were Candida albicans and Sclerotinia sclerotiorum. The antibiofilm activity was differentiated and depended on the extraction conditions, the microorganism and the method of biofilm treatment. All tested extracts inhibited biofilm formation, with the extract obtained at 40 °C showing the highest value, whereas only extract obtained at 60 °C efficiently removed mature biofilm.

Effect of process variables in supercritical carbon dioxide extraction of tocotrienols from hydrolyzed palm fatty acid distillate ( PFAD )

The recovery of tocotrienols from palm fatty acid distillate (PFAD), a by-product of palm oil, is of economic and environmental interest. In this study tocotrienols were extracted from PFAD using supercritical carbon dioxide (scCO2) at different temperatures (40–60°C) and pressures (20–40 MPa). The optimal process conditions were estimated to be 20 MPa and 53°C for 300 min and a CO2 flow rate of 32 ± 2 g/min, with 16.45 mg/g of tocotrienols extracted. Two modifications to the extraction process were then made at the optimal conditions. First, 180 min of static extraction was added before the dynamic extraction; and second, the solvent polarity was altered by adding 0.075 ml/g ethanol to the scCO2 system. These modifications significantly increased the tocotrienol extraction, to 23.62 mg/g and 30.03 mg/g, respectively. The use of scCO2 for the extraction of tocotrienols shows great potential, which might be extended to other compounds. Novelty impact statement scCO2 extraction is capable to extract tocotrienols from palm fatty acid distillate (PFAD) at optimum condition by altering the density of CO2 through manipulating pressure and temperature. The extraction of tocotrienols can be enhanced my slight modification on the scCO2 system or modifying the polarity of the CO2 solvent. The best recovery for tocotrienols extraction from PFAD was by adding 0.075 ml/g ethanol in the scCO2 system.

Pilot-Scale Optimization of Supercritical CO2 Extraction of Dry Paprika Capsicum annuum: Influence of Operational Conditions and Storage on Extract Composition

Supercritical carbon dioxide extraction was used to extract carotenoids from dry paprika Capsicum annuum. Studies regarding the effect of process parameters, including pressure (25–45 MPa), temperature (40–60 °C), and time (10–110 min), were carried out using response surface methodology. It was found that under optimal conditions (pressure of 45 MPa, temperature of 50 °C, and time of 74 min), the extract yield was 10.05%, and the total content of carotenoids in the extract was 4.21%, in good agreement with the predicted values (10.24% and 4.24%, respectively). Composition analysis showed that paprika extract mainly consisted of linoleic acid. There was no significant difference between the fatty acid content of the extracts obtained by SC-CO2 extraction and n-hexane Soxhlet extraction. For functional purposes, the effect of storage conditions and time on the quality of paprika extract was also specified.

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats.

Compared to conventional artificial nerve guide conduits (NGCs) prepared using natural polymers or synthetic polymers, acellular nerve grafts (ACNGs) derived from natural nerves with eliminated immune components have natural bionic advantages in composition and structure that polymer materials do not have. To further optimize the repair effect of ACNGs, in this study, we used a composite technology based on supercritical carbon dioxide (scCO2) extraction to process the peripheral nerve of a large mammal, the Yorkshire pig, and obtained an innovative Acellular nerve xenografts (ANXs, namely, CD + scCO2 NG). After scCO2 extraction, the fat and DNA content in CD + scCO2 NG has been removed to the greatest extent, which can better supported cell adhesion and proliferation, inducing an extremely weak inflammatory response. Interestingly, the protein in the CD + scCO2 NG was primarily involved in signaling pathways related to axon guidance. Moreover, compared with the pure chemical decellularized nerve graft (CD NG), the DRG axons grew naturally on the CD + scCO2 NG membrane and extended long distances. In vivo studies further revealed that the regenerated nerve axons had basically crossed the CD + scCO2 NG 3 weeks after surgery. 12 weeks after surgery, CD + scCO2 NG was similar to autologous nerves in improving the quality of nerve regeneration, target muscle morphology and motor function recovery and was significantly better than hollow NGCs and CD NG. Therefore, we believe that the fully decellularized and fat-free porcine ACNGs may be the most promising “bridge” for repairing human nerve defects at this stage and for some time to come.

Microscopic remaining oil initiation mechanism and formation damage of CO2 injection after waterflooding in deep reservoirs

Injecting CO2 into deep reservoirs with high temperature and pressure (HTHP) is a promising method for enhancing oil recovery (EOR) and CO2 utilization and storage (CUS). It is, however, challenging to quantitatively unravel the initiation mechanisms of micro-remaining oil and formation damage using microfluidic technology at 115 °C and 55 MPa. In this study, water/gas flooding experiments in the micromodel has carried out on the HPHT microfluidic experimental platform. The results reveal that 35.41% of the micro-remaining oil is recovered by scCO2 extraction on the funnel-shaped interface, and 11.21% by carbonated water swelling. When water is injected into heterogeneous reservoirs, the oil-water displacement front in the high-permeability zone is less stable than that in the low-permeability zone. Subsequent scCO2 injection resulted in asphaltene deposits (approximately 10.49% of pore volume), including low-velocity deposition, corner deposition and trap deposition, which reduced reservoir permeability. Moreover, compared with continuous CO2 injection, the asphaltene deposition is reduced by 4.25% of CO2 injection after waterflooding, including 2.64% in the high-permeability zone and 1.61% in the low-permeability zone. These observations provide important theoretical support for the application of CO2-EOR technology and carbon storage in deep reservoirs.

Extrusion affects supercritical CO2 extraction of red pepper (Capsicum annuum L.) oleoresin

Carotenoids provide red colour and antioxidant properties in formulated foods. High-value, carotenoid-rich oleoresin can be extracted from red pepper using SuperCritical (SC) carbon dioxide (CO2). Densifying the solid substrate can overcome process limitations of batchwise operation to increase the extraction vessel load of red pepper. However, there is no information in literature on the effect of densifying treatments on the bioactive components of vegetable matrices. This work evaluated the effect of extrusion as a densification process on the yield and quality of SC–CO2–extracted red pepper oleoresin. A 23-factorial experimental design was used to study the effects of substrate pretreatment (control or extruded) and extraction temperature (40 or 60 °C) and pressure (30 or 50 MPa) on SC-CO2 extraction yield, and the extractable colour (ASTA units), carotenoid content, antioxidant activity (inhibition of a DPPH radical), and oxidative index (p-anisidine value) of the extracts. Extrusion had a significant (p ≤ 0.01) but negative effect on all responses. Independent of substrate pretreatment, SC-CO2 extraction yield and carotenoid yield where maximized at the extreme conditions of 60 °C and 50 MPa. The extractable colour and antioxidant activity correlated positively (p ≤ 0.05), and their oxidative index correlated negatively (p ≤ 0.05) with the carotenoid content of the SC-CO2 extracts.

Evaluation of the antioxidant, anti-inflammatory and antihyperglycemic activities of black bean (Phaseolus vulgaris L.) by-product extracts obtained by supercritical CO2

Food by-products have been used as source of bioactive compounds with health benefits. Supercritical carbon dioxide (SC-CO2) extraction has been a valuable technology to obtain a diversity of bioactive compounds with the use of safer solvents. This research focused on the comparison of the antioxidant, anti-inflammatory and antihyperglycemic activities of SC-CO2 and conventional extracts obtained from black bean by-products. A Box-Behnken design was used to evaluate the effect of temperature, pressure, and co-solvent on the soyasaponins recovery. Total phenolic compounds (TPC) were quantified using Folin-Ciocalteu. Cellular antioxidant activity (CAA) and anti-inflammatory response via nitric oxide (NO) assay were evaluated in vitro. The antihyperglycemic activity was evaluated using digestive enzymes. The highest soyasaponin extraction yield was obtained at 70 °C, 10 MPa and 10% co-solvent. SC-CO2 extracts exhibited up to 74.7% of CAA, an inhibition of 93.0% NO, 25.1% α-amylase, 78.7% α-glucosidase and 82.33 ± 5.80 of TPC. SC-CO2 extraction resulted in a feasible technology to obtain high-value compounds from industrial by-products to treat hyperglycemic, oxidative stress and inflammation.