Scavenging Activity Research Articles

Bio-prospecting the Anti-oxidative and Radioprotective Role of Bioactive Pigment Isolated from Pontibacter indicus

Aim: To evaluate the anti-oxidative and radioprotective role of Pontibacter indicus SCG24 pigment during radiation exposure Background: Radiation-induced cytotoxicity is quite common during cancer therapy. There is a need for naturally derived therapeutic molecules that can scavenge free radicals. They may act as substitutes for synthetic molecules. Hence, there is a need for urgent evaluation of these potent compounds before therapeutic application. Objective: The objective of this study is to examine the anti-oxidative and radioprotective role of P. indicus SCG24 pigment, specifically to evaluate free radical scavenging X-ray irradiated HDF cells Method: A radiotoleraent pigment-producing P. indicus SCG24 was isolated from pharmaceutical effluent. Chloroform was used as a primary solvent for pigment extraction. GCMS/MS analysed initial pigment composition. Various In-vitro antioxidant assays were performed using ABTS, FRAP, and DPPH assay. Flow cytometry was used to determine the rate of scavenging activity of pigment in HDF cells. Results: The GCMS/MS profile of the chloroform extract revealed twenty-two compounds. Furthermore, based on the DPPH, ABTS, and FRAP assay, the pigment was found to have significant antioxidant properties. The flow cytometry results indicate that the pigment possesses radioprotectant activity by neutralizing ROS species in HDF cells when exposed to X-ray radiation. Conclusion: These observations on P. indicus SCG24 pigment suggested that the pigment may have potential therapeutic importance.

Phytochemical composition analysis, antioxidant, antimitotic, and anti-inflammatory effects of leaf and stem extracts of Pistacia lentiscus L

ABSTRACT This study explored the phytochemical composition, antioxidant properties, anti-germinative effects, and anti-inflammatory actions of methanolic leaf extracts (MLE) and methanolic stem extracts (MSE) derived from Pistacia lentiscus L. species native to the eastern region of Morocco. Using Ultra-high-performance liquid chromatography (UHPLC),16 and 17 components were discovered inMLE and MSE, respectively, with Epigallocatechin being the most prevalent in both extracts. Polyphenols and flavonoids were found to be more abundant in MLE. Furthermore, DPPH assays revealed that it had a higher free radical scavenging activity (IC50 = 37.5 mg/L) compared to ascorbic acid while ferric-reducing power tests showed that MSE had a greater potency (IC50 = 86.14 mg/L) than MLE. Both MLE and MSE inhibited sorghum seed germination in a dose-dependent manner, with MSE being slightly more effective. Mitotic index determination revealed that both extracts had concentration-dependent antimitotic effects, with MSE showing similarity to colchicine, indicating its potential as an antimitotic agent. In vitro, anti-inflammatory assays showed that it had similar properties to indomethacin in protein denaturation and membrane stabilization, while MLE displayed distinct characteristics. Furthermore, in a carrageenan-induced rat model, both extracts demonstrated anti-inflammatory potential, with MSE showing a trend of slightly better efficacy. These results highlight the importance of further investigation into MSE’s potential as an anti-inflammatory and antimitotic agent.

Fortification of yogurt with cloves extract

The purpose of the current study was to evaluate the impact of different concentrations of clove extract (CE: 0%, 0.5%, 1.0%, and 2.0%) on the viscoelasticity, viscosity, syneresis, texture, sensory quality, and antioxidant properties of set yogurt up to 21 days in cold storage. The addition of CE enlarged the total suspended solids (TSS) and mineral contents while reducing whey separation. On the other hand, CE existence dropped the consistency index (k) and elastic modulus (G’) of yogurt gels and further lessening was observed with prolonged storage period. Moreover, the textural firmness remained unaffected whereas gels that are more cohesive were attained with integration of CE. Similarly, total phenolics and free radical scavenging activity increased significantly (p < 0.05) by CE supplementation, and amplified lipid oxidation was observed with increased storage time. Addition of CE lowered the lightness (L*) index and manipulated the organoleptic properties whereas the sample with 1% CE showed improvement in texture and flavor; however, the overall acceptability reduced for samples with higher concentration of CE.

Metabolite Profiles and Biological Activities of Different Phenotypes of Beech Mushrooms (Hypsizygus marmoreus).

Beech mushrooms (Hypsizygus marmoreus) are edible mushrooms commercially used in South Korea. They can be classified into white and brown according to their pigmentation. This study analyzed the metabolites and biological activities of these mushrooms. Specifically, 42 metabolites (37 volatiles, two phenolics, and three carbohydrates) were quantified in white beech mushrooms, and 47 (42 volatiles, two phenolics, and three carbohydrates) were detected in brown mushrooms. The major volatiles detected were hexanal, pentanal, 1-hexanol, and 1-pentanol. Brown mushrooms contained higher levels of hexanal (64%) than white mushrooms (35%), whereas white mushrooms had higher levels of pentanal (11%) and 1-pentanol (3%). Most volatiles were more abundant in white mushrooms than in brown mushrooms. Furthermore, brown beech mushrooms had a higher phenolic content than white mushrooms. Biological assays revealed that both types of mushroom demonstrated anti-microbial activities against bacterial and yeast pathogens and weak DPPH scavenging activity. The extracts from both mushrooms (50 μg/mL) also exhibited strong anti-inflammatory properties. Brown mushroom extracts showed higher antioxidant, anti-microbial, and anti-inflammatory properties than white mushroom extracts. This study reported that the differences in phenotype, taste, and odor were consistent with the metabolite differences between white and brown beech mushrooms, which have high nutritional and biofunctional values.

Comprehensive evaluation of ibuprofenate amino acid isopropyl esters: insights into antioxidant activity, cytocompatibility, and cyclooxygenase inhibitory potential.

Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for pain relief and inflammation management, but there are challenges related to poor solubility and bioavailability. We explored modifications of ibuprofen (IBU) by forming ionic pairs using amino acid alkyl esters to enhance solubility without compromising the ability to inhibit cyclooxygenase (COX)-1 and COX-2). We comprehensively evaluated the pharmacological properties of the IBU derivatives, focusing on antioxidant activity (based on the ability to scavenge DPPH and ABTS), biocompatibility (using human dermal fibroblasts), and COX inhibitory potential. The antioxidant activity assays significantly enhanced DPPH scavenging activity for several IBU derivatives, particularly [L-SerOiPr][IBU], suggesting potential therapeutic benefits. There was enhanced cell viability with select derivatives, indicating possible stimulatory effects on cellular proliferation. Finally, predominant COX-1 inhibition across derivatives was consistent with IBU's profile. This study provides insights into the pharmacological properties of IBU amino acid derivatives, highlighting their potential as therapeutic agents. Further exploration into structure-activity relationships and in vivo efficacy warranted to advance these derivatives toward clinical applications, offering prospects for novel NSAIDs with enhanced efficacy and reduced side effects.

Antioxidative and Anti-Atopic Dermatitis Effects of Peptides Derived from Hydrolyzed Sebastes schlegelii Tail By-Products.

Atopic dermatitis (AD) is a chronic inflammatory skin disorder associated with significant morbidity, including pruritus, recurrent skin lesions, and immune dysregulation. This study aimed to investigate the antioxidative and anti-AD effects of peptides derived from hydrolyzed Sebastes schlegelii (Korea rockfish) tail by-products. Hydrolysates were prepared using various enzymes, including Alcalase, Flavourzyme, Neutrase, and Protamex. Among them, Protamex hydrolysates demonstrated the highest ABTS radical scavenging activity with an RC50 value of 69.69 ± 0.41 µg/mL. Peptides were further isolated from the Protamex hydrolysate using dialysis, fast protein liquid chromatography (FPLC), and high-performance liquid chromatography (HPLC). The most active peptide, STPO-B-II, exhibited a single peak and was identified as a sequence of Glu-Leu-Ala-Lys-Thr-Trp-His-Asp-Met-Lys, designated as MP003. In vivo experiments were conducted using a 2,4-dinitrochlorbenzene (DNCB)-induced AD model in NC/Nga mice. The isolated peptide, MP003, showed significantly reduced AD symptoms, including erythema, lichenification, and collagen deposition. Additionally, MP003 decreased epidermal and dermal thickness, eosinophil, and mast cell infiltration and downregulated the expression of pro-inflammatory cytokines IL-1β, IL-6, and IgE in serum and skin tissues. These findings suggest that peptides derived from Sebastes schlegelii tail by-products may serve as potential therapeutic agents for AD.

Bioactive Lipids in Dunaliella salina: Implications for Functional Foods and Health.

Dunaliella salina is a green microalga extensively explored for β-carotene production, while knowledge of its lipid composition is still limited and poorly investigated. Among lipids, polar lipids have been highlighted as bioactive phytochemicals with health-promoting properties. This research aimed to provide an in-depth lipidome profiling of D. salina using liquid and gas chromatography coupled with mass spectrometry. The lipid content was 6.8%, including phospholipids, glycolipids, betaine lipids, sphingolipids, triglycerides, diglycerides, and pigments. Among the total esterified fatty acids, 13.6% were 18:3 omega-3 and 14.7% were 18:1 omega-9. The lipid extract of D. salina showed anti-inflammatory activity by inhibiting cyclooxygenase-2 activity at 100 µg/mL, dose-dependent antioxidant scavenging activity, and antidiabetic activity by inhibiting α-glucosidase activity at 25 and 125 µg/mL. In conclusion, the lipid extract of D. salina has the potential to be used as a functional food ingredient or in the nutraceutical and cosmeceutical industries.

Radical Scavenging Capacity and In Vitro Cytoprotective Effects of Great Salt Lake-Derived Processed Mineral Water.

The Great Salt Lake, located in Utah, USA, is a saltwater lake with no outlet and is surrounded by vast mountains and salt deserts. We aimed to use Great Salt Lake-derived processed mineral water (hereafter termed as GSL-MW) for maintaining oral health. Therefore, we examined its radical scavenging activity as an antioxidant and its cytoprotective effect on human gingival fibroblasts (hGFs). The scavenging activity against O2•- radicals was determined by an electron spin resonance (ESR)-spin trapping technique using two kinds of O2•- generation systems; however, we could not reach any concrete conclusion because of the interference caused by GSL-MW in both systems. Detection of ·OH radicals using the ESR-spin trapping technique and kinetic analyses using double-reciprocal plots (corresponding to Lineweaver-Burk plots that are used to represent enzyme kinetics) revealed that GSL-MW has the ability to scavenge ·OH radicals. GSL-MW also showed a weak 2,2-diphenyl-1-picrylhydrazyl (DPPH; a stable radical)-scavenging activity. Regarding the cytoprotective effects, subconfluent hGFs pretreated with 10× and 100× dilutions of GSL-MW for 3 min and then exposed to harsh environmental conditions, such as pure water or 100 μM H2O2 for 3 min, showed enhanced cell viability rate. Moreover, 10× and 100× dilutions of GSL-MW reduced oxidative damage in confluent hGFs exposed to 12.5 and 25 mM H2O2. Our findings show that GSL-MW has antioxidant potential and cytoprotective effects on hGFs, suggesting that GSL-MW can be used to maintain oral health.

Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian Ganoderma Species.

Lanostene-derived triterpenoids and β-glucans are important metabolites in Ganoderma mushrooms associated with benefits to human health. The medicinal value of the Australian Ganoderma species remains unclear, with no data on triterpenoid distribution or glucan content. In the present study, 22 Australian Ganoderma specimens were analyzed for triterpenoid and glucan contents. Thirty-two triterpenoids were identified in the fruiting bodies of 19 of the specimens. Distinct patterns in triterpenoid distribution between laccate and matte fruiting bodies were observed, leading to the classification of four groups of Ganoderma. Most of the glucans in the Ganoderma fruiting bodies were β-glucans (~99%), with a nominal α-glucan content (~1%). The β-glucan content ranged from 19.5 to 43.5% (w/w). A range of antioxidant activities was observed for methanol extracts using the ABTS (1.8 to 8.4 mg GAE.g-1), DPPH (1.7 to 9.4 mg GAE/g-1) and FRAP (24.7 to 111.6 mmol FeSO4.g-1) assays, with four specimens presenting relatively high radical scavenging and reducing activities. For the first time, we demonstrated that Australian Ganoderma mushrooms contain medicinal triterpenoids, including ganoderic acid A, and we established a link between its distribution and the fruiting body morphology. However, further research is required to isolate diploid clones and determine factors that impact triterpenoid and glucan synthesis in these strains.

Bioactive phenolic contents of Scorzonera ketzkhowelii Sosn. ex Grossh. (Asteraceae) with comprehensive in vitro and in silico studies

This study explores the antioxidant, anti-inflammatory, and anticholinesterase properties of extracts from the natural plant Scorzonera ketzkhowelii for the first time. Additionally, it focuses on isolating phenolic compounds from the ethyl acetate sub-extract, elucidating their structures, and investigating their in-silico bioactivities. Twelve phenolic compounds were isolated and characterized from the ethyl acetate sub-extracts, including hydrangenol (1), 4-hydroxybenzaldehyde (2), luteolin (3), esculin (4), 3-O-caffeoylquinic acid ethyl ester (5), 3-O-caffeoylquinic acid methyl ester (6), kaempferol 3-O-β-glucopyranoside (7), quercetin 3-O-α-arabinopyranoside (8), 3,5-di-O-caffeoylquinic acid ethyl ester (9), thunberginol F 7-O-β-D-glucopyranoside (10), hydrangeic acid 4′-O-β-D-glucopyranoside (11), and 3-O-caffeoylquinic acid (12). The ethyl acetate sub-extracts from both aerial and subaerial parts demonstrated exceptional radical scavenging activity. Moreover, all fractions exhibited potent inhibition against COX-I and COX-II enzymes, with notable inhibitory effects observed in the ethyl acetate and dichloromethane sub-extracts against AChE. Additionally, the inhibitory effects of these compounds were assessed against various biological targets, including TNFα, COX-I, COX-II, human CYP450, and hAChE, through molecular docking studies. According to the molecular docking and dynamics studies, compound 9 emerged as particularly noteworthy across all complexes, exhibiting stable binding modes and promising interactions with key residues involved in inhibition.

New Quinazolin-4(3H)-One Derivatives Incorporating Isoxazole Moiety as Antioxidant Agents: Synthesis, Structural Characterization, and Theoretical DFT Mechanistic Study.

Background: This research centers on the development and spectroscopic characterization of new quinazolin-4(3H)-one-isoxazole derivatives (5a-e). The aim was to investigate the regioselectivity of the 1,3-dipolar cycloaddition involving arylnitriloxides and N-propargylquinazolin-4(3H)-one, and to assess the antioxidant properties of the synthesized compounds. The synthetic approach started with the alkylation of quinazolin-4(3H)-one using propargyl bromide, followed by a 1,3-dipolar cycloaddition reaction. Methods: The structural identification of the products was performed using various spectroscopic methods, such as IR, 1H, 13C, and HMBC NMR, HRMS, and single-crystal X-ray diffraction. To further examine the regioselectivity of the cycloaddition, Density Functional Theory (DFT) calculations at the B3LYP/6-31G(d) level were employed. Additionally, the antioxidant potential of the compounds was tested in vitro using DPPH (2,2-Diphenyl-1-picrylhydrazyl)radical scavenging assays. The reaction selectively produced 3,5-disubstituted isoxazoles, with the regiochemical outcome being independent of the substituents on the phenyl ring. Results: Theoretical calculations using DFT were in agreement with the experimental results, revealing activation energies of -81.15 kcal/mol for P-1 and -77.32 kcal/mol for P-2, favoring the formation of P-1. An analysis of the Intrinsic Reaction Coordinate (IRC) confirmed that the reaction proceeded via a concerted but asynchronous mechanism. The antioxidant tests demonstrated that the synthesized compounds exhibited significant radical scavenging activity, as shown in the DPPH assay. The 1,3-dipolar cycloaddition of arylnitriloxides with N-propargylquinazolin-4(3H)-one successfully resulted in novel 3,5-disubstituted isoxazoles. Conclusions: The experimental findings were well-supported by theoretical predictions, and the antioxidant assays revealed strong activity, indicating the potential for future biological applications of these compounds.

Revealing the Potential of Star Anise Essential Oil: Comparative Analysis and Optimization of Innovative Extraction Methods for Enhanced Yield, Aroma Characteristics, Chemical Composition, and Biological Activities

ABSTRACTStar anise has been used for a long time in improving human health and curing diseases, owing to its unlimited components with complex chemical structures and a wide range of bioactivities. This study is aimed to investigate the influence of extraction methods (steam distillation, ethanol Soxhlet extraction, supercritical carbon dioxide extraction, subcritical n‐butane extraction) on the yield, aroma properties, chemical composition, and bioactivity of star anise essential oils. Electronic nose detection revealed the essential oils from subcritical extraction exhibited the most intense aroma, while the essential oils from ethanol Soxhlet extraction had a more complex aroma profile. Fourier‐transform infrared analysis showed the presence of benzene rings, carbonyl groups, C=C, and aromatic ether bonds in the essential oils extracted through different methods. The major components were heterocyclic olefins, heterocyclic oxygenates, and aromatic oxygenates, as well as certain amounts of flavonoids and polyphenols. Correlation analysis revealed the relative contents of volatile trans‐anethole, wormwood, d‐limonene, cineole, and trans‐α‐citronelene were strongly associated with the antibacterial activity of the essential oils. Similarly, the contents of volatile components (d‐limonene, cineole) and non‐volatile components (total flavonoids and total polyphenols) were strongly correlated with the DPPH scavenging activity of the essential oils. These results confirm the effectiveness of the ethanol Soxhlet extraction method in retaining the bioactivity of the essential oils. Finally, with a Box–Behnken central composite design of response surface and single‐factor experiments, the optimal extraction conditions for the ethanol Soxhlet method were determined: ultrasonic frequency of 80 kHz, crushing particle size of 60 meshes, liquid–material ratio of 8:1 (mL:g), and ultrasonic time of 35 min. Under these conditions, the essential oil yield was 25.51% ± 0.21%. Overall, these findings highlight the significance of extraction methods in obtaining high‐quality star anise essential oils with desirable aroma properties and potent bioactivities.

Evaluating the nutrient composition and antioxidant properties of orange (Citrus sinensis) peels through Penicillium camemberti-based solid-substrate fermentation

Citrus by-products, especially orange peels, are a significant global waste issue, driving research into management solutions. Solid-substrate fermentation is a promising method to convert this waste into valuable resources, enhancing its medicinal and nutritional potential while addressing environmental and economic concerns in food waste management. Therefore, this study was carried out to evaluate the impact of solid-substrate fermentation (SSF) with Penicillium camemberti on the nutrient profile, antioxidative properties, and potential neuroprotective effects of orange peels in AlCl3 (Al)-induced neurotoxicity in fruit fly (Drosophila melanogaster) model. Orange peels were collected, air-dried to a constant weight, and subjected to a 10-day SSF process with Penicillium camemberti. Post-fermentation, in vitro nutritional profiling, and antioxidant assays were conducted, alongside in vivo assessments of the neuroprotective effects on survival rate, memory index, monoamine oxidase (MAO) activity, and endogenous antioxidant markers on Al-induced fruit flies were determined. The results showed that fermentation with Penicillium camemberti significantly enhanced the nutritional composition of the orange peels compared to their unfermented counterparts. The antioxidant properties of fermented orange peels were significantly (p < 0.05) increased, as evidenced by increased DPPH scavenging activity (81.68 ± 0.18) and ferric reducing antioxidant power (10.77 ± 0.27). Additionally, biochemical assays in vivo revealed a significant improvement in survival rates and memory indices in Al-induced flies. Also, monoamine oxidase (MAO) levels were significantly reduced, while glutathione S-transferase (GST) levels were significantly (p < 0.05) elevated in the induced flies fed with the fermented samples. Conclusively, this study highlights the capability of Penicillium camemberti fermentation in converting waste into valuable resources, enhancing the nutrient profile of citrus peels for applications in livestock feed and potential therapeutic interventions for degenerative disorders.

Anti-Oxidant and Anti-Cancerous Properties of Pyrus pashia (Buch. -Ham.Ex D.Don) Against KB-3-1 Cancer Cell Lines In Vitro

Background: Oral cancer is the fastest-growing cancer in men. Many medicinal approaches are tried step-wise for cancers. Homeopathy and Ayurveda are contributing much to cure cancers without side effects. Plants having antioxidant properties are now being utilized with different solvents to extract different chemical compounds to cure cancer. Objective: The current study aimed to examine the anti-cancer and antioxidant properties of acetone extract of Pyrus pashia (PP-AE) fruit against KB-3-1 cancer cell lines. Materials and Methods: The extract's total phenolic content was determined using a folin-ciocalteu reagent. The DPPH, ABTS, and H2O2 assay were used to measure the free radical scavenging activity of the PP-AE. The MTT assay was used to evaluate the cytotoxic efficacy of the PP-AE against KB-3-1 cancer cell lines at various concentrations. Results: Based on the observations, the total phenolic content was present in the PP-AE of fruit and all free radical scavenging assays have shown remarkable IC50 values. Conclusion: According to the current study, acetone extract of Pyrus pashia fruit significantly possesses antioxidant and anti-cancer properties against oral (KB-3-1) cancer cell lines. This effect may be used in the future to analyze particular chemical compounds in extract, capable of minimizing the growth of oral cancer cells.

Chemical profiling and bioactivity analysis of shoots and roots essential oil of Indian Blumea mollis D. Don (Merr.)

Aromatic plants contain essential oils, potent extracts renowned for therapeutic benefits. Essential oils are commonly utilized due to their diverse range of phytochemicals that possess therapeutic properties. The present study analyses the chemical composition of the essential oil obtained from shoots and roots of Blumea mollis (Family: Asteraceae), using GC-FID and GC-MS techniques. A total of 49 and 46 compounds were identified from shoots and roots accounting for 95.6 % and 88.4 % respectively. β-caryophyllene is reported as major compound 36.2 ± 0.50 % in essential oil of the shoots and 33.8 ± 0.55 % in essential oil of the roots. Both essential oils of shoots and roots were dominated by sesquiterpene hydrocarbons (59.2–76.4%), followed by oxygenated sesquiterpene (14.9–20.3%). The in vitro antioxidant activity was determined using DPPH radical scavenging activity, H2O2 radical scavenging activity, and Iron(II) complexing activity. The highest antioxidant activity was observed in root essential oil of B. mollis. The oils also inhibited the activity of α-amylase with IC50 of 3.53 ± 0.04 μg/ml (shoot), 3.45 ± 0.04 μg/ml (root). Root oil also showed good activity for protein denaturation with IC50 of 3.02 ± 0.03 g/ml as compared to shoot essential oil. This is first time that essential oil constituent and biological activity of B. mollis roots and shoots have been characterized from India.

Solvent assisted mechanochemical synthesis, antimicrobial and antioxidant studies of Ni2+, Mn2+, Cr&lt;sup&gt;2+&lt;/sup&gt; , and Cu&lt;sup&gt;2+&lt;/sup&gt; Metal complexes of some ligands derived from l-histidine and l-alanine

Mechanochemistry enables rapid, quantitative reactions to occur at or close to 27℃. It is a one-step process that allows for reactant homogenization, product nucleation, and particle growth. These processes use little or no solvent, making them less wasteful and more environmentally friendly than solvent-based reactions. Ni2+ , Mn2+, Cr2+and Cu2+ metal ions were synthesized by grinding in an agate mortar with pestle using ligands derived from amino acids (Histidine &amp;Alanine) which were further synthesized to form complexes. The entire experiment was carried out by solvent solvent-assisted mechanochemical process and ethanol was used as liquid liquid-assisted solvent. The complexes were characterized by IR spectroscopy, U-V spectroscopy, X-ray analysis, melting point, solubility test, and conductivity measurement. The Solubility test showed that the complexes are soluble in DMSO, DMF, and ethanol and are insoluble in both hexane and tetrachloromethane. The molar conductivity values are in the range of (6.05-28.56) Ω-1cm2mol-1 ) indicating the complexes behave as non-electrolytes. IR analysis showed a vibrational frequency band observed at (1620-1625) cm-1 which is assigned to the ʋ(C=N) of the azomethine, also the new bands M=N, M=O indicate the coordination reaction between the metal and the ligands. The UV visible analysis shows various transitions, the metal complexes showed adsorption bands at λ of 420 and 575nm respectively because of the ligand-to-metal charge transfer (LMCT). The XRD analysis showed that there was a change in phase between the starting material and the product which indicate that a reaction occurred, also the nature of the spectra showed that the compounds are all crystalline. The Antimicrobial activity showed that both the complexes and the ligands have good activity against the bacterial isolates. Antioxidant activity was also tested and the compound appeared to have moderate scavenging activities compared to the reference used. The values indicated that the activities are more pronounced when coordinated with metal ions.

In silico studies, synthesis, characterization and in vitro studies of levosulpiride derivatives.

Aim: Breast cancer is the most recurring cancer among females and is being diagnosed as a major cause of death among women.Materials & methods: Levosulpiride Schiff base derivatives were synthesized and analyzed by physical and spectral (FTIR, 1H-NMR, 13C-NMR) analysis. MTT assay against MCF-7 (human breast cancer cell line), scavenging activity and Molecular docking against receptors 1M17, 3PP0, 3IOK and 4KIK along ADME pharmacokinetic studies were performed.Results & conclusion: L1 and L3 synthesized derivatives have revealed better percent cell viability and inhibitory concentration (IC50) with scavenging activity as of the parent compound. L1, L3 and L9 revealed significant docking scores compared with standard drugs. Most of the derivatives showed strong pharmacokinetic profiles while no drug crossed blood-brain barrier. The newly synthesized L1 and L3 levosulpiride-derived compounds have demonstrated promising anticancer properties against breast cancer cells.

Short-term tank culture of Gracilariopsis heteroclada at high salinities improves agar quality

Abstract Agar, a phycocolloid naturally extracted from Gracilariopsis heteroclada is strongly influenced by salinity. Wild stocks of G. heteroclada were exposed to varying salinities (20, 30, 40, 50, 60) for 6 days, and subsequently processed for agar extraction using 5 % NaOH. The extracted agar was evaluated regarding yield, gel rheology, colour composition, chemical properties, and infrared spectra. Results highlighted that hypersaline conditions (salinity 50) could produce high agar yield (4.77 %) and viscosity (10.67 mPa s), while agar samples at salinity 40 exhibited gels with high cohesiveness (6.35 mm), gel breaking strength (3.01 N), and gel strength (390.61 g cm−2) while having a rather high 3,6-anhydrogalactose content (7.49 %). All samples exhibited FTIR signature peaks at 930 cm−1, confirming the identity of extracted agar from G. heteroclada. Exposure at increasing hypersalinity increased the sulphate levels of agar from G. heteroclada, which implies synthesis of sulphated polysaccharides. Moreover, high 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities were obtained in acid hydrolysed agars at salinities of 40 (74.09 %) and 50 (75.57 %), suggesting that G. heteroclada agars from hypersaline conditions potentially offer antioxidative roles beyond its traditional food use.

Effects of Different Cooking Methods on Physicochemical and Antioxidant Properties of Climbing Perch (Anabas Testudineus)

Climbing perch, Anabas testudineus is a freshwater fish recognised for its distinct flavour, high nutritional content, and medicinal properties. This species can be found in almost every Asian country, including Malaysia. However, the cooking methods applied can change the nutritional value of the climbing perch. This study aims to investigate the effect of four different cooking methods for climbing perch, which are frying, grilling, steaming and air frying, on their cooking loss, texture, colour properties, proximate composition, free fatty acid, total phenolic contents and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The result showed that the physicochemical properties, proximate composition and antioxidant activity were significantly (P&lt;0.05) affected by the cooking method. Fried climbing perch samples were significantly higher (P&lt;0.05) for cooking loss and texture. The steamed sample has the lightest colour with 40.39, and the fried sample has the highest value in redness and yellowness with 6.76 and 11.40 respectively. Proximate composition showed the highest value in protein, fat and carbohydrate in fried samples. Furthermore, free fatty acid was highest in the steamed sample (1.98%) and the lowest in the fried sample (1.39%). Fried samples showed the highest total phenolic content (TPC), 1.23 mg GAE/g and DPPH scavenging activity was highest in steamed samples (69.62%). In conclusion, aside from frying, steamed method emerged as the effective cooking method for preserving the nutritional value of the fish, as it exhibited the highest values in most of the analyses conducted.

OPTIMIZATION ULTRASOUND-ASSISTED EXTRACTION USING CHOLINE CHLORIDE-BASED NATURAL DEEP EUTECTIC SOLVENT TO INCREASE PHENOLIC COMPOUNDS AND ANTIOXIDANTS FROM RHODOMYRTUS TOMENTOSA LEAVES

Objective: The main purpose of this study is to give recommendations for the ideal extraction conditions for improving the extraction yield and antioxidant activity of R. tomentosa leaves. Methods: First, the extraction total phenolic yields of five choline chloride-based Natural Deep Eutectic Solvents (NADES) were evaluated. Then, Box Behnken designs of Response Surface Methodology (RSM) were conducted in order to optimize the extraction condition. The extraction variables investigated were extraction time, water content in NADES, and solid-to-liquid ratio. Meanwhile, total phenolic and 2,2′-azinbis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radicals scavenging activity were used as responses. Results: NADES with combination of choline chloride and propylene glycol with a molar ratio of 1:1 was found to be the best solvent for extracting phenolic compound. The total phenolic compound obtained was 29.6351 mg GAE/g dried leaves with ABTS scavenging activity about 96.84% under the optimum extraction condition (extraction time of 60 min, 25% water content in NADES, and solid-to-liquid ratio of 0.02 g/ml). Better results were shown compared to extracts with conventional solvents. Conclusion: In sum, the use of choline chloride-based NADES as extraction solvent under optimum condition was proven to be effective in increasing the extraction efficiency of phenolic compounds and antioxidant activity from R. tomentosa leaves.