An innovative analytical procedure for the analysis of polycyclic aromatic hydrocarbons (PAHs) from large-volume water samples is presented. It involves sample preparation, sampling and the elution process in an automated continuous procedure involving the ASE technique. Prior to sampling, a XAD-2 resin column is prepared on the basis of a commercial accelerated solvent extraction (ASE) cartridge so that the resin bed is permanently fixed. Then, the XAD column inside the ASE cartridge is cleaned and conditioned. The sampling procedure involves conventional filtration with subsequent isolation of dissolved PAHs on an XAD-2 resin contained in the ASE cartridge. After sampling, the XAD-2 resin content inside the cartridge is eluted by ASE without any further sample preparation and subsequently reused. In order to validate the procedure, the PAHs were isolated from water samples from the Lake Maggiore (North of Italy) using both XAD-2 resin adsorption and hexane liquid–liquid extraction according to the International Standard Methodology ISO 17993. The mean percentages of deviation between concentrations obtained by both methodologies range from 6% for benzo( a)pyrene to 15% for fluoranthene and benzo( b, k)fluoranthene. Compared to the traditional techniques, this procedure offers numerous practical advantages: easy to perform, fast, savings in solvent volume and in time, all steps are fully automated thus avoiding any XAD-2 resin manipulation during and between steps and moreover, low detection limits were provided (0.001 ng l −1 for chrysene, benzo( b, k)fluoranthene, benzo( a)pyrene, dibenz( a, h)anthracene, benzo( g, h, i)perylene and indeno(1,2,3- cd)pyrene, and 0.01 ng l −1 for acenaphthylene and fluoranthene). This procedure was developed in the frame of a project aimed at evaluating the diffuse input of organic contaminants in the Lake Maggiore.