Saussurea Lappa Research Articles

Saussurea costus for sustainable and eco-friendly synthesis of palladium nanoparticles and their biological activities

Palladium nanoparticles have been evaluated as a viable candidate in the realm of biological applications due to their unique features. Saussurea costus extract was used as a stabilizing and reducing agent for the synthesis of palladium nanoparticles with average grain size of 17.6 ± 1.2 nm. The synthesized PdNPs were evaluated for their antioxidant activity, anti Alzheimer's activity, antibacterial and anticancer activities. The nanocharacterization was carried out using different spectroscopic techniques, including UV–visible spectroscopy, Transmission Electron Microscopy, Fourier Transformed Infrared spectroscopy, X-ray Diffraction analysis, X-ray Photoelectron spectroscopy, Energy Dispersive X-ray Spectroscopy, Size distribution, and Zeta potential. The characterization data explained the PdNPs mediated by S.costus extract have spherical form and are disseminated without agglomeration. FTIR and XPS supported the hypotheisis that the biomolecules of S.costus are activing as a reducing and stabilizing agents. The antioxidant activity of PdNPs was assessed using a free radical scavenging assay (DPPH) which exhibited similar results to the ABTS assay i.e. 90 μg/mL IC50 value. Moreover Alzheimer's disease can easily be inhibited by S.costus@PdNPs at 400 mg/mL, with 79.23 ± 1.11 % of inhibition rate against AChE and 76.13 ± 0.43 % towards BChE. S.costus@PdNPs showed comparatively greater antibacterial activity against all four Staphylococcus aureus, Bacillus subtilis Escherichia coli and Pseudomonas aeruginosa microorganisms. Supplementary research carried out on the anti-tumor effects of the generated PdNPs using the colon cancer (HCT-116), hepatocellular carcinoma (HepG2), and breast adenocarcinoma (MCF-7) cell lines. PdNPs showed potent anticancerous activity against all the cell lines. Thus we recommend S.costus@PdNPs as a thearapeutic agent after successful clinical trails in future.

Treatment with Rosemarinus Officinalis Leaves and Saussurea Lappa Roots Extracts Ameliorate Hepatorenal and Cardiac Cisplatin Toxicity in Ehrlich Ascitic-Bearing Mice

Medicinal plants have important role in cancer treatments and protect the vital organs from the adverse effect of chemotherapy treatments. This study was conducted to investigate the ameliorating effects of Rosemarinus officinalis leaves (ROLE) and Saussurea lappa roots (SLRE) extracts along with cisplatin (Cis) on hepato-renal and cardiac-toxicities induced in Ehrlich-bearing mice. Forty female Swiss albino mice were divided into 4 groups (n=10) as follows; the first group (Gp1) was served as control. Gp2-Gp4 were inoculated with 1 x 106 EAC-cells/mouse. Gp2 was left without any treatment as EAC-bearing mice, and Gp3 had injected with Cis (2 mg/Kg) starting from day 3 to day7. Gp4 had injected with Cis as in Gp3, and then administered with ROLE/ SLRE from day 3 to day 13. At day 14, blood samples were collected and heart tissue homogenate was prepared for biochemical analysis. Serum hepatic enzymes; alanine aminotransferase (ALT), aspartate aminotransferase (AST) ɤ-glutamyltransferase (ɤ GGT), bilirubin, creatinine and urea levels were significantly increased in EAC-bearing mice, however, the treatment of EAC-bearing mice with Cis/ROLE/SLRE decreased the mentioned biochemical parameters. The contents of troponin (TnI) and creatine-phosphokinase (CPK) in the heart tissue of EAC-bearing mice were also increased; however, the treatment with Cis/ROLE/SLRE exhibited a marked ameliorative effect on both Tn1 and CPK. These results suggested that administration of ROLE/SLRE along with Cis mitigated the toxicity in EAC-bearing mice

In vitro anticandidal activity of the secondary metabolites extracted from Saussurea Costus (Falc.) lipschitz roots

The present study, was conducted to investigate the effect of the crude Alkaloids, Flavonoids, and Terpenoids compounds extract from roots of (Saussurea costus) against Candida species isolated from different clinical samples such as mouth and vagina in the province of Babil 2021 in Iraq. Anticandidal activity was achieved in vitro by using agar well diffusion method against Candida species by preparing three concentrations for each crude compound (25, 50, and 100) mg/ml and compared to positive control represented by Fluconazole 50mg/ml and negative control represented by 10% dimethyl sulfoxide. The aim of this study was to control of Candida species isolated different clinical samples such as mouth and vagina by using secondary metabolites extracted from seeds of Carthamus tinctorius L. The data collected from the study revealed that, the crude Alkaloids, Flavonoids, and Terpenoids compounds extract from seeds of (Saussurea costus) showed reduction at P≤ 0.05 in the growth of Candida species especially at 100mg/ml compared with negative control. Finally, it can be concluded that Saussurea costus is most effective in controlling Candida species, especially Terpenoid and Alkaloids compounds.

Mechanisms of pancreatic tumor suppression mediated by Xiang-lian pill: An integrated in silico exploration and experimental validation

Ethnopharmacological relevanceXiang-lian pill, consisting of Coptis chinensis Franch. coprocessed with Tetradium ruticarpum (A.Juss.) T.G.Hartley (Yu-huang-lian) and Aucklandia lappa DC. (Mu-xiang), is traditionally used to relieve fever, abdominal pain, and gastrointestinal inflammatory symptoms observed in patients with malignancies of the gastrointestinal tract. Each of the three herbs contained in Xiang-lian pill has been indicated to have anticancer effects on a variety of cancers, but its effects on pancreatic cancer remain unexplored. The main extracts of these herbs have anti-pancreatic cancer effects, but the comprehensive mechanism of this compound prescription of Xiang-lian pill in pancreatic cancer remains to be revealed. Aim of the studyTo explore the main active ingredients, potential anti-pancreatic cancer targets, and related mechanisms of the Xiang-lian pill and to determine its therapeutic value in vivo. Materials and methodsNetwork pharmacology and bioinformatics analysis were applied to screen the potential effective ingredients and key targets. Liquid/gas-mass spectrometry was performed for ingredients validation. Molecular docking and the cellular thermal shift assay were performed to test the binding efficiency between ingredients and targets. A murine pancreatic cancer model was established and administered different doses of the Xiang-lian pill. Hematoxylin-eosin staining was used for histopathological observation. Immunohistochemistry and immunoblotting were conducted for target validation. In vitro studies (cell viability and clonogenicity assays) were conducted to investigate the impact of three main ingredients in Xiang-lian pill on pancreatic cancer cells. PTGS2 overexpression was performed to reversely confirm the antitumor mechanisms of rutaecarpine as a specific PTGS2 inhibitor. ResultsXiang-lian pill suppressed pancreatic cancer growth in the dose range of 0.78-2.34g/kg with no significant toxicity. Sixteen potentially active ingredients and 26 corresponding therapeutic targets for pancreatic cancer were identified. PTGS2, PTGS1, KCNH2, PRSS1, and HSP90AA1 were the top 5 significant genes targeted by the Xiang-lian pill. Evodiamine, rutaecarpine and stigmasterol bound to PTGS2 and PTGS1 with different affinities and inhibited pancreatic cancer cell proliferation. The PTGS2-associated metabolic pathway MEK/ERK was downregulated by rutaecarpine in vitro and the Xiang-lian pill in vivo. ConclusionsXiang-lian pill mainly regulates inflammation, apoptosis, metastasis, and metabolism to exert an antitumor effect. The main active ingredients in Xiang-lian pill exhibit antitumor roles through directly binding to key targets in pancreatic cancer. PTGS2 mediated MEK/ERK inhibition by rutaecarpine represents a key therapeutic mechanism of Xiang-lian pill.

Phytochemical Discrimination, Biological Activity and Molecular Docking of Water-Soluble Inhibitors from Saussurea costus Herb against Main Protease of SARS-CoV-2.

Siddha medicine is one of the oldest medical systems in the world and is believed to have originated more than 10,000 years ago and is prevalent across ancient Tamil land. It is undeniable that inhibitor preferences rise with increasing solubility in water due to the considerations pertaining to the bioavailability and the ease of which unabsorbed residues can be disposed of. In this study, we showed the phytochemical discrimination of Saussurea costus extracted with water at room temperature as a green extraction procedure. A total of 48 compounds were identified using gas chromatography-mass spectrometry (GC-MS). The fatty acids had a high phytochemical abundance at 73.8%, followed by tannins at 8.2%, carbohydrates at 6.9%, terpenoids at 4.3%, carboxylic acids at 2.5%, hydrocarbons at 2.4%, phenolic compounds at 0.2%, and sterols at 1.5%. Of these compounds, 22 were docked on the active side and on the catalytic dyad of His41 and Cys145 of the main protease of SARS-CoV-2 (Mpro). Eight active inhibitors were carbohydrates, five were fatty acids, three were terpenoids, two were carboxylic acids, one was a tannin, one was a phenolic compound, and one was a sterol. The best inhibitors were 4,8,13-Cyclotetradecatriene-1,3-diol, 1,5,9-trimethyl-12-(1-methylethyl), Andrographolide, and delta.4-Androstene-3.beta.,17.beta.-diol, with a binding affinity that ranged from −6.1 kcal/mol to −6.5 kcal/mol. The inhibitory effect of Saussurea costus of SARS-CoV-2 entry into the cell was studied using a pseudovirus with Spike proteins from the D614G variant and the VOC variants Gamma and Delta. Based on the viral cycle of SARS-CoV-2, our results suggest that the Saussurea costus aqueous extract has no virucidal effect and inhibits the virus in the events after cell entry. Furthermore, the biological activity of the aqueous extract was investigated against HSV-1 virus and two bacterial strains, namely Staphylococcus aureus ATCC BAA 1026 and Escherichia coli ATCC 9637. According to this study, an enormous number of water-soluble inhibitors were identified from Saussurea costus against the Mpro, and this is unprecedented as far as we know.

Antioxidant and Anti-Urolithiatic Activity of Aqueous and Ethanolic Extracts from Saussurea costus (Falc) Lispich Using Scanning Electron Microscopy.

The plant Saussurea costus (Falc) Lipsch has many biological activities and a strong curative and preventive power against a variety of diseases including cancer, diabetes, and hemorrhoids. In the current study, phytochemical screening was carried out as well as an investigation of the antilithiatic and antioxidant activities of aqueous and ethanolic extracts of this plant. The results showed that aqueous and ethanolic extracts were effective in reducing cystine stone mass and that the aqueous extract of Saussurea costus (Falc) Lipsch had the highest percentage of dissolution (6.756 ± 1.024) (p < 0.05). A turbidimetric method and a crystallization test were used to evaluate the antilithiatic activity of an aqueous and ethanolic extract of this plant on calcium oxalate crystallization. The results of these methods revealed that the ethanolic extract of this plant has a significant inhibitory effect on calcium oxalate crystallization, with a percentage inhibition of (91.017 ± 0.299) (p < 0.05) for a concentration of 2 mg mL−1. The DPPH method revealed that the ethanolic extract of Saussurea costus (Falc) Lipsch with a concentration of (IC50 = 0.12325 mg mL−1) had the highest IC50, whereas the FRAP method revealed that the aqueous extract of Saussurea costus (Falc) Lipsch with a concentration of 300 µg mL−1 has the most significant reducing power with (OD = 0.56 ± 0.05). These findings indicate that aqueous and ethanolic extracts of Saussurea costus (Falc) Lipsch had a significant effect on whewellite and weddellite and a greater free radical scavenging effect but had no effect on cystine dissolution.

Development and Physico-Chemical Analysis of Varnya Lepa Sunscreen Lotion (In-Vitro)

Background: Direct exposure to Solar radiation causes many harmful effects on the skin like suntan, sunburn, hyperpigmentation and skin ageing etc. To overcome these types of problems sunscreen applications can be helpful. In Ayurveda, authors explained Varnya Lepa drugs like Raktachandana (Pterocarpus santalinus Linn.), Manjistha (Rubia cordifolia Linn), Lodhra (Symplocos racemosa Roxb), Kustha (Saussurea lappa), Priyangu (Callicarpa macrophylla), Vatankura (Ficus benghalensis) and Masura (Lens culinaria Medic) have a high potential due to their antioxidant activity, vitamin, photoprotective, flavonoids and phenolic acid in the safety of the skin from UV rays and blocks each UVA and UVB. This formulation is mentioned for external application in the form of Lepa but due to problems like stains after applications, impediment in utilization, protection trouble, non-accessibility of crisp medication all the season. If Lepa contains herbal drugs it should be used within 24 hours, otherwise, the drugs get decomposed and the application may harm the skin. Lotion is viscous semisolid preparation. They may be oil-in-water or water-in-oil type, easy to apply in unbroken skin without friction. Lotions contain water-soluble bases and hence are more convenient to use. Aim: The present study aim was to develop Varnya Lepa sunscreen lotion and Assessment of Sun Protection Factor (SPF) by In-vitro method. Material and Methods: A lotion base of Four trials was done, in this trial fourth was passed all parameters and the final development of sunscreen lotion was prepared. The quantity of extract used in sunscreen lotion was selected after assessment of the absorbance factor in UV-spectrophotometry and calculated sun protection factor. Results: The Sun protection factor was obtained 29 and shown under broad-spectrum sunscreen. Conclusion: Varnya Lepa sunscreen lotion was successfully developed. Hence, can be used as sunscreen lotion.

Activity of Various Fractions of Saussurea Lappa Herb Against Multidrug Resistant Gram Negative Rods

Background: Microorganisms are turning out to be greatly resistant to existing antibiotics, specifically gram-negative rods which shows resistance to currently accessible antibiotics. Beta-lactam antibiotics are the main therapeutic option to treat infections of gram-negative microorganisms i.e., ESBL and Carbapenemase producers. Saussurea lappa herb is a medicinal herb use since many times. Basically, the roots of S. lappa herb were used as medicines. Current study was conducted to find out activity of various fractions of S. lappa herb against multidrug resistant gram-negative rods. Methods: Crude extracts of ethanol, methanol and water and fractions of n-hexane, chloroform, and methanol from S. lappa herb were used. Total of sixty multidrug resistant organisms were included in which thirty were ESBL and thirty were carbapenemase producing organisms. Mean MIC and comparative analysis of various extracts and fractions have been evaluated. Results: The mean MIC value of crude extracts of ethanol, methanol, n-hexane, chloroform and methanol fractions and water extract from S. lappa against ESBL producing organisms were 109.33± 6.915 mg/ml, 154.67± 5.164 mg/ml, 150.00± 5.345 mg/ml, 55.33±5.164 mg/ml, 178.00±6.103 mg/ml, 64.00± 7.701 mg/ml respectively. Similarly, the mean MIC value against carbapenemase producing organisms were 100.67± 8.683 mg/ml, 158.67± 3.519 mg/ml, 150.67± 5.936 mg/ml, 54.67±5.164 mg/ml, 176.67±14.223 mg/ml, 64.33± 9.353 mg/ml respectively. Conclusion: This study suggests that extracts and fractions of S. lappa herb can form the basis to develop novel broad-spectrum formulation for antimicrobial drugs as it contains compounds that has novelty to perform its action against multi-resistant mechanisms.

EFFECT OF KUSHTHADI GHRITA IN ADOLESCENT MENTAL HEALTH PROBLEMS: A REVIEW

Adolescence is the transitional phase for mental health promotion since more than half of mental health problems begin during this time, and many of them remain into adulthood. These conditions account for 10-20% of the global disease burden. This impacts the individual's likelihood of having an intellectual disability. According to Ayurveda, acharya Kashyap describes a Kushthadi ghrita prepared with Kushtha (Saussurea lappa), leaf-buds of Vata (Ficus benghalensis), Gauri (yellow mustard) (Sinapis alba), Pippali (Piper longum), Triphala [Haritaki (Terminalia chebula), Amalaki (Phyllanthus emblica), Bibhitaki (Terminalia bellirica)] Vacha (Acorus calamus), and rock-salt which is one of the best drugs for improving mental functions (Nootropic activity). The present paper reviews the indications and uses of Kushthadi ghrita described in Ayurveda texts. Studies published in various journals regarding the evidence of its ingredient effect are also examined. This Kushthadi ghrita drug acts as a Medhya drug and Rasayana, which is beneficial for improving mental functions. Medhya drugs ultimately increase the overall cognitive capacity of the brain, i.e., by increasing perfusion and metabolism and improving the chemical imbalance in the brain. Rasayana drugs supposed to increase all the sharira dhatu. The ingredients of these drugs have Antioxidant, Anxiolytic, Antistress activity, antidepressant, Neuroprotective, and Anti-amnestic Properties, which are supportive for improving mental functions (Nootropic activity).

The exploration of bio-inspired copper oxide nanoparticles: synthesis, characterization and in-vitro biological investigations

The paper describes the synthesis and characterization of copper oxide nanoparticles (CuO NPs) using the mixture of plant rhizome extracts Ocimum sanctum and Saussurea lappa as a reducing agent. The prepared CuO nanoparticles are characterized and confirmed their formation based on data obtained from powder X-ray diffraction spectroscopy, Fourier Transmission Infrared, Ultraviolet-Visible spectra, Field Emission Scanning Electron Microscopy images, Energy Dispersive X-ray analysis and Dynamic light scattering techniques and data reveal that the average size of CuO Nps was 103.4 nm. The result of antibacterial and antifungal activities for concentrations 50, 100, and 170 ppm indicate that NPs may exhibit appreciable activity at higher (170 ppm) concentrations. The MTT cytotoxic assay studies of Chinese Hamster Ovary (CHO) cell lines showed a Half-maximal inhibitory concentration (IC50) value of 4.14 ​μg/mL.

Sesquiterpene lactones-rich fraction from Aucklandia lappa Decne. alleviates dextran sulfate sodium induced ulcerative colitis through co-regulating MAPK and Nrf2/Hmox-1 signaling pathway

Ethnopharmacological relevanceAucklandia lappa Decne. (ALDE) is the general name for Asteraceae plants Yunmuxiang, which has traditionally been proven to have the efficacy in relieving depression by regulating qi, alleviating cold by warming, attenuating pain in stomach and relieving diarrhea in intestines. Therefore, ALDE is always recommended as an herbal remedy for gastrointestinal dysfunction. Aim of the studyThe purpose of this study was to explore the therapeutic potential and mechanism of action of the sesquiterpene lactone-rich fraction (SLRF) of ALDE extracts in vivo and in vitro. Materials and methodsAn aqueous extract (AE) and SLRF of ALDE were prepared and the contents of the main components were quantified by high performance liquid chromatography (HPLC). The therapeutic effects of the extracts were evaluated in C57BL/6 mice with dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). Body weight, disease activity index (DAI), and colon length were recorded, and histopathological changes in the colon were characterized using hematoxylin and eosin (H&E) staining. The in vitro anti-inflammatory activity and possible mechanisms of the two main sesquiterpene lactones in ALDE (costunolide and dehydrocostus lactone) were studied by quantitative proteomic analysis. Finally, based on bioinformatic analysis, we used polymerase chain reaction (PCR), immunofluorescence, and western blot experiments to verify the anti-inflammatory mechanism of the extracts in C57BL/6 mice. ResultsThe SLRF of ALDE significantly improved the pathological symptoms and inflammatory pathology of UC, whereas the AE had a weak protective effect. In RAW264.7 cells stimulated with lipopolysaccharide (LPS), costunolide and dehydrocostus lactone significantly reduced the mRNA levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, suggesting that these two sesquiterpene lactones had strong anti-inflammatory activity. Quantitative proteomics results indicated that the anti-inflammatory mechanism of these lactones was associated with the NF-κB/MAPK and Nrf2-Hmox-1 pathways. These results were further validated in SLRF-treated mice. ConclusionThis study confirmed that the SLRF of ALDE exerted protective activity against UC by regulating the Nrf2-Hmox-1, NF-κB, and MAPK pathways.

Antimicrobial activity of Saussurea costus extracts against Streptococcus penumoniae and Escherichia coli

Traditionally, numerous components of medicinal plants, such as their roots, stems, flowers, fruits, and twigs, were widely employed as crude extracts to treat many human ailments. This investigation aimed to look at the effectiveness of various antimicrobial agents of Saussurea costus against G+ve bacteria Streptococcus penumoniae and G-ve bacteria Escherichia coli. The antibacterial activity of the plant was determined using the diffusion technique, which was used to both alcoholic and aqueous extracts generated from the plant's roots against streptococcus penumoniae and Escherichia coli; the inhibition zone was measured by millimeter. The antimicrobial examination revealed that the Gram-positive bacteria represented by Streptococcus penumoniae were susceptible to the alcoholic extracts of S. costus with inhibition zones (18 mm) at 200 mg/ml concentration. In contrast, the aqueous extract had fewer inhibition zones (16 mm) at a 200 mg/ml concentration. Meanwhile, E.coli had a weak effect on the alcoholic extracts with inhibition zones (13 mm) at 200 mg/ml concentration. Still, aqueous extract was (10 mm) at200 mg/ml concentration. The constituents of the ethanolic extract of roots were investigated using Gas chromatography-mass (GC); twenty-four compounds were identified in the extract of the roots, such as C10H14 and C10H16. The results demonstrated that the Saussurea costus extracts have antimicrobial activity against Gram-positive bacteria S. pneumoniae while it has a weak effect against E.coli Keywords Saussurea costus. Antimicrobial. Ethanolic extract. Streptococcus penumoniae. Escherichia coli

Enhanced antimycotic activity of nanoconjugates from fungal chitosan and Saussurea costus extract against resistant pathogenic Candida strains

Targeting the control of pathogenic Candida spp., especially the fungicides resistant strains from C albicans and C glabrata, nanoconjugates from the biopolymer (chitosan) and costus root extract (Saussurea costus) was synthesized and characterized. Chitosan was extracted from the grown mycelia of Aspergillus niger and characterized with high deacetylation degree of 91.2% and moderate molecular weight of 106.8 kDa. Synthesis of nanoconjugates from fungal chitosan/costus extract (NCt/CE) was conducted using ionic gelation technique; the resulted NCt/CE particles were characterized with mean diameter of 48 nm, positive zeta potentiality (+3.28 mV) and high stability. The infra‐red spectra of synthesized nanoconjugates indicated their strong biochemical cross‐linkage. The antimycotic activities, of the synthesized NCt, CE and their nanocomposite, were evaluated against standard and antibiotic‐resistant strains from C albicans and C. glabrata and revealed that the entire agents had notable antimycotic potentiality against all examined strains; the NCt/CE nanoconjugates had significantly stronger antimicrobial action. The scanning microscope imaging, of exposed resistant strains to NCt/CE, indicated their vigorous structural and morphological alterations and confirmed the antimycotic activity of the nanocomposite. NCt/CE nanoconjugates' synthesis could be exceedingly recommended as a natural, biodegradable and effectual antimycotic agent to control resistant pathogenic yeast strains.

Tentatively Identified (UPLC/T-TOF–MS/MS) Compounds in the Extract of Saussurea costus Roots Exhibit In Vivo Hepatoprotection via Modulation of HNF-1α, Sirtuin-1, C/ebpα, miRNA-34a and miRNA-223

Saussurea costus is a plant traditionally used for the treatment of several ailments. Our study accomplished the UPLC/T-TOF–MS/MS analysis of a methanol extract of Saussurea costus roots (MESC), in addition to lipoidal matter determination and assessment of its in vivo hepatoprotective activity. In this study, we were able to identify the major metabolites in MESC rather than the previously known isolated compounds, improving our knowledge of its chemical constituents. The flavones apigenin, acacetin, baicalein, luteolin, and diosmetin, and the flavonol aglycones quercetin, kaempferol, isorhamnetin, gossypetin, and myricetin and/or their glycosides and glucuronic derivatives were the major identified compounds. The hepatoprotective activity of MESC was evaluated by measuring catalase activity using UV spectrophotometry, inflammatory cytokines and apoptotic markers using ELISA techniques, and genetic markers using PCR. Paracetamol toxicity caused a significant increase in plasma caspase 2, cytokeratin 18 (CK18), liver tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), miRNA-34a, and miRNA-223, as well as a significant decrease in liver catalase (CAT) activity and in the levels of liver nuclear factor 1α (HNF-1α), sirtuin-1, and C/ebpα. Oral pretreatment with MESC (200 mg/kg) showed a significant decrease in caspase 2, CK18, TNF-α, IL-6 and a significant increase in liver CAT activity. MESC decreased the levels of liver miRNA-34a and miRNA-223 and induced HNF-1α, sirtuin-1, and C/ebpα gene expression. The histological examination showed a significant normalization in rats pretreated with MESC. Our findings showed that Saussurea costus may exert a potent hepatoprotective activity through the modulation of the expression of cellular cytokines, miRNA-34a, and miRNA-223.

Foresight on Phytoconstituents and Associated Pharmacological Activities of Traditional Medicinal Plant: Saussurea costus (Falc.) Lipschitz

Foresight on Phytoconstituents and Associated Pharmacological Activities of Traditional Medicinal Plant: Saussurea costus (Falc.) Lipschitz

Saussurea Costus Extract Has Anti-inflammatory, Antioxidant, and Hormonal Effects Against Testicular Toxicity Induced by Oxaliplatin in Male Albino Rats

Background: Testicular dysfunction is one of the common side effects that results from the treatment with oxaliplatin® as a chemotherapy drug, and pharmaceutical search for agents to relieve the side effects are underway. The current study explored the possible ameliorative and regenerative effects of Costus ethanolic extract against testicular degeneration in male rats induced by oxaliplatin. Methods: Male Wistar albino rats weighing 150-200g were divided into four groups of 10 rats each as follows: group-1 control rats; group-2 rats treated orally with the extract at 50 mg/kg/day for six weeks; group-3 rats injected oxaliplatin intraperitoneally at 10 mg/kg/week for six successive weeks; group-4 rats were injected intraperitoneally with oxaliplatin at 10 mg/kg/week combined with a daily oral dose of the Costus extract for six weeks. Results: Data from the current study revealed that the extract lowered the toxic effect of oxaliplatin on the testicular tissue samples. This was evident by the significant rise in the serum of total &amp; free testosterone and CD4 cells, and the levels of GSH, SOD and CAT activities in the testis coupled with a marked reduction of serum TNF-α and IL-1β and testis MDA, nitric oxide levels and DNA fragmentation. Also, the extract promoted the regeneration of the histopathological structure of the testis. Conclusion: This study proposes a novel therapeutic application for the Costus extract as a therapeutic agent against testicular toxicity induced by oxaliplatin treatment through its promising anti-inflammatory and antioxidant properties.

Structure-Based Molecular Networking for the Discovery of Anti-HBV Compounds from Saussurea lappa (Decne.) C.B Clarke.

It is a crucial to find target compounds in natural product research. This study presents a concept of structure-guided isolation to find candidate active molecules from herbs. We establish a process of anti-viral sesquiterpene networking. An analysis of the networking suggested that new anti-HBV sesquiterpene may be attributable to eudesmane-, guaiane-, cadinane-, germacane- and bisabolane-type sesquiterpenes. In order to evaluate the efficiency of the structure-based molecular networking, ethanol extract of Saussurea lappa (Decne.) C.B Clarke was investigated, which led to the isolation of two guaiane-type (1 and 14), ten eudesmane-type (2–5 and 8–13), two chain (6 and 7) and one germacrane-type (15) sesquiterpenes, including seven new ones, lappaterpenes A–G (1–7), which are reported on herein. The absolute configurations of the new compounds were established by coupling constants, calculated ECD and ROESY correlations, as well as comparisons of optical rotation values with those of known compounds. The absolute configuration of compound 2 was further confirmed by X-ray diffraction. Compounds 1–15 were evaluated for their potency against hepatitis B virus. Compounds 4, 6, 7 and 9 showed effect on HBsAg with inhibition ratios of more than 40% at 30 μM concentrations. Compounds 14 and 15 inhibited HBsAg secretion with the values of IC50 0.73 ± 0.18 and 1.43 ± 0.54 μM, respectively. Structure-based molecular networking inspired the discovery of target compounds.

IN VITRO EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF FIVE HERBAL EXTRACTS AGAINST STREPTOCOCCUS MUTANS

Background: The emergence and extend of antibiotic resistance, together with the development of new strains of illness causes, are of enormous alarm to the global health community. Successful treatment of a illness involves the development of new pharmaceuticals or some promise source of novel drugs. Universally make use of medicinal plants of our community could be an outstanding source of drugs to fight off dental caries. This study is focused on discovering the antibacterial properties of the plants that are frequently being used as traditional medications. Methods: Five methanol extracts from Salvia officinalis, Commiphora myrrha, Boswellia carteril, Saussurea lappa and Dracaena cinnabari were examined for their antibacterial activities against most common bacterial oral pathogen, Streptococcus mutans. The antibacterial testing was carried out by using the disc diffusion and broth micro-dilution assays. Results: Methanol extracts of the five plants were effective against Streptococcus mutans with diameter zone of inhibition ranging from 63.6 to 21.0 mm. The results of the microdilution assay confirmed that the Salvia officinalis, Commiphora myrrha, Saussurea lappa and Dracaena cinnabari were effective against the Streptococcus mutans, exhibiting MIC values, ranging from 0.310 to 0.156 mg/ml. Conclusion: The results of our study indicate that the methanol extracts of plants used in this study have an antibacterial effect even at low concentration against the carcinogenic Streptococcus mutans bacteria, and they may be possible to combat Streptococcus mutans to increase the effectiveness of oral hygiene practices by incorporating the extracts of these plants into anti-caries such as Toothpastes and mouthwash. Peer Review History: Received: 18 January 2022; Revised: 13 February; Accepted: 8 March, Available online: 15 March 2022 Academic Editor: Dr. Asia Selman Abdullah, Pharmacy institute, University of Basrah, Iraq, asia_abdullah65@yahoo.com UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewers: Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, alialkaf21@gmail.com Similar Articles: ASSOCIATION BETWEEN THE STREPTOCOCCUS MUTANS BIOFILM FORMATION AND DENTAL CARIES EXPERIENCE AND ANTIBIOTICS RESISTANCE IN ADULT FEMALES ANALYSIS OF BIOFILMS FOR STREPTOCOCCUS MUTANS FROM DENTAL ROOT SURFACES OF ADULT PATIENTS WITH ROOT CARIES

Dehydrocostus Lactone Suppresses Dextran Sulfate Sodium-Induced Colitis by Targeting the IKKα/β-NF-κB and Keap1-Nrf2 Signalling Pathways.

Dehydrocostus lactone (DCL) is a major sesquiterpene lactone isolated from Aucklandia lappa Decne, a traditional Chinese herbal medicine that used to treat gastrointestinal diseases. This study aimed to examine the therapeutic effects of DCL on dextran sulfate sodium (DSS)-induced colitis with a focus on identifying the molecular mechanisms involved in DCL-mediated anti-inflammatory activity in macrophages. First, oral administration of DCL (5–15 mg/kg) not only ameliorated symptoms of colitis and colonic barrier injury, but also inhibited the expression of proinflammatory cytokines and myeloperoxidase in colon tissues in DSS-challenged mice. Furthermore, DCL also exhibited significant anti-inflammatory activity in LPS/IFNγ-stimulated RAW264.7 macrophages. Importantly, DCL significantly suppressed the phosphorylation and degradation of IκBα and subsequent NF-κB nuclear translocation, and enhanced the nuclear accumulation of Nrf2 in LPS/IFNγ-treated RAW264.7 cells. Mechanistically, DCL could directly interact with IKKα/β and Keap1, thereby leading to the inhibition of NF-κB signalling and the activation of Nrf2 pathway. Furthermore, DCL-mediated actions were abolished by dithiothreitol, suggesting a thiol-mediated covalent linkage between DCL and IKKα/β or Keap1. These findings demonstrated that DCL ameliorates colitis by targeting NF-κB and Nrf2 signalling, suggesting that DCL may be a promising candidate in the clinical treatment of colitis.

Botany, traditional uses, phytochemistry and pharmacological properties of Saussurea costus – An endangered plant from Himalaya- A review

• The paper will provide knowledge of phytochemistry, traditional uses, and pharmacological properties, of Saussurea costus . • Further, will provide comprehensive knowledge regarding the availability and cultivation of Saussurea costus in India. • Different compounds isolated from Saussurea costus may provide a new therapeutic approach for the administration of various diseases. • It paves a way for the future researchers for identification and isolation of novel drugs. Saussurea costus (Falc.) Lipsch. Syn . Saussurea lappa C.B. Clarke belongs to family Asteraceae and is a critically endangered plant that is monetarily very vital as a therapeutic herb of North - West Himalaya. Saussurea costus has been traditionally used to treat a variety of ailments in different indigenous health systems viz. , carminative, expectorant, antiarthritic, antiseptic, aphrodisiac, anodyne and vermifuge without any noticeable adverse effects. Textbooks on ethnobotany, peer-reviewed academic publications, unpublished materials, and research repositories were used to collect the widespread review of literature on Saussurea costus . The scientific names were authenticated by using Kew Botanical Garden, International Plant Name Index and Plant List databases. Costunolide, dihydrocostunolide, sesquiterpene lactones and cynaropicrin have been identified as major phytoconstituents of Saussurea costus with great potency to develop as a drug candidate. Different pharmacological experiments convincingly confirmed the potential of Saussurea costus with anticancer, anti-ulcer, hepatoprotective activities, etc. providing support to the various traditional uses associated with Saussurea costus . Saussurea costus will be a preferable alternative for novel drug discovery due to its notable pharmacological properties. Although the existing information to far indicates the security and possibly efficacy of Saussurea costus, bioactive ingredients, physiological mechanisms, pharmacokinetics, accessibility, and the impact on human health are not fully understood. The present review piles up the up-to-date and widespread information on botany, traditional uses, phytochemistry and pharmacology of Saussurea costus , to assess its therapeutic potential, with a focus on in vitro , biomedical, and histopathological experiments.