Treatment with Rosemarinus Officinalis Leaves and Saussurea Lappa Roots Extracts Ameliorate Hepatorenal and Cardiac Cisplatin Toxicity in Ehrlich Ascitic-Bearing Mice

Abstract

Medicinal plants have important role in cancer treatments and protect the vital organs from the adverse effect of chemotherapy treatments. This study was conducted to investigate the ameliorating effects of Rosemarinus officinalis leaves (ROLE) and Saussurea lappa roots (SLRE) extracts along with cisplatin (Cis) on hepato-renal and cardiac-toxicities induced in Ehrlich-bearing mice. Forty female Swiss albino mice were divided into 4 groups (n=10) as follows; the first group (Gp1) was served as control. Gp2-Gp4 were inoculated with 1 x 106 EAC-cells/mouse. Gp2 was left without any treatment as EAC-bearing mice, and Gp3 had injected with Cis (2 mg/Kg) starting from day 3 to day7. Gp4 had injected with Cis as in Gp3, and then administered with ROLE/ SLRE from day 3 to day 13. At day 14, blood samples were collected and heart tissue homogenate was prepared for biochemical analysis. Serum hepatic enzymes; alanine aminotransferase (ALT), aspartate aminotransferase (AST) ɤ-glutamyltransferase (ɤ GGT), bilirubin, creatinine and urea levels were significantly increased in EAC-bearing mice, however, the treatment of EAC-bearing mice with Cis/ROLE/SLRE decreased the mentioned biochemical parameters. The contents of troponin (TnI) and creatine-phosphokinase (CPK) in the heart tissue of EAC-bearing mice were also increased; however, the treatment with Cis/ROLE/SLRE exhibited a marked ameliorative effect on both Tn1 and CPK. These results suggested that administration of ROLE/SLRE along with Cis mitigated the toxicity in EAC-bearing mice