The third generation of the BeiDou navigation satellite system (BDS-3) can transmit five-frequency signals. The real-time satellite clock offset of BDS-3 is typically generated utilizing the B1I/B3I combination with the ambiguity-float solutions. By conducting the ambiguity resolution (AR), the reliability of the satellite clock offset can be improved. However, the performance of BDS-3 ambiguity-fixed real-time satellite clock offset with B1C/B2a signals remains unknown and unrevealed. In this contribution, the performance of the BDS-3 ambiguity-fixed satellite clock offset with the new B1C/B2a signals is investigated. One week of observation data from 85 stations was used to perform ambiguity-fixed satellite clock offset estimation. For B1I/B3I and B1C/B2a signals, the wide-lane (WL) uncalibrated phase delay (UPD) on the satellite end is fairly stable for one day, while the narrow-lane (NL) UPD standard deviation (STD) amounts to 0.122 and 0.081 cycles, respectively. The mean ambiguity fixing rate is 80.7% and 78.0% for these two signal combinations, and the time to first fix (TTFF) for the B1C/B2a signals is remarkably shorter than that of the B1I/B3I signals. The STDs of the ambiguity-float and -fixed satellite clock offsets are 0.033 and 0.026 ns, respectively, for the B1I/B3I combination, and it is reduced to 0.024 and 0.023 ns for B1C/B2a signals, respectively. Using the estimated UPD and clock offset products, the positioning performance of the kinematic Precise Point Positioning (PPP)-AR results amounts to 1.56, 1.23, and 4.46 cm in the east, north, and up directions for B1I/B3I signals, respectively. It is improved to 1.36, 1.16, and 4.25 cm using the products estimated with the B1C/B2a signals, with improvements of 12.8%, 5.7%, and 4.7% in three directions, respectively. The experiments showed that the performances of the ambiguity-fixed satellite clock offsets and the PPP-AR results using B1C/B2a signals are better than those of B1I/B3I.