Sarcoma-180 Tumor Research Articles

Biological effects of anthramycin methyl ether. II. Inhibition of DNA synthesis in the intestinal epithelium and sarcoma-180 tumor cells.

The temporal course of DNA synthesis after subcutaneous injection on Anthramycin Methyl Ether (AME) was investigated in Sarcoma-180 ascites tumor cells and in the proliferative cells of the intestinal epithelium. Both cell types demonstrated dramatic inhibition of DNA synthesis: however, there was no appreciable difference in response of the 2 cell systems to the drug. After sub-lethal doses, recovery was seen to occur in an oscillatory fashion which eventually reached control values. This pattern of recovery suggested partial <i>in vivo </i>synchronization of the proliferative cell population, which could be due to preferential block in one stage of cell cycle or selective killing of cells in a sensitive stage.

Inhibition of DNA synthesis in sarcoma-180 tumor cells in vitro by dimethyl sulfoxide.

SummaryDMSO was found to inhibit DNA synthesis in S-180 ascites tumor cells in vitro. This inhibition was a linear function of DMSO concentration from 5 to 15% (v/v). Removal of the DMSO resulted in an immediate restoration of the capacity for TdR-3H incorporation.

Studies on the Anti-Tumor Activity of Polysaccharides. I. Isolation of Hemicelluloses from Yakushima-bamboo and Their Growth Inhibitory Activities against Sarcoma-180 Solid Tumor

In order to confirm the chemical entities of the anti-tumor substances in the extract of the leaves of a sort of bamboo, Sasa senanensis (FR. et SAV.) REHD, a series of fractionation was carried out on the macromolecular constituents of the extract. Two hemicelluloses were isolated as homogeneous states both in free boundary electrophoresis and ultracentrifugal analysis. One of them was a neutral polysaccharide which was precipitable with alkaline copper solution and consisted of L-arabinose, D-xylose and D-glucose, while the other contained D-galactose in addition to the three sugar components mentioned above and showed weak acidity due to a small amount of uronic acid. Both polysaccharides showed over 90% tumor-inhibitions when injected intraperitoneally into mice bearing Sarcoma-180 solid tumor.

Sequential Synthesis of Two 7 S Lysins by Rats Challenged with Mouse Sarcoma-180 Tumor Cells

Summary The administration of S-180 mouse ascites tumor cells to rats has been shown to stimulate the production of two distinct lysins capable of rupturing the tumor cells in vitro. The two antibodies were separated on diethylaminoethyl (DEAE)-cellulose and were shown by sucrose density gradient centrifugation and by chromatography on Sephadex G-200 to be 7 S antibodies. No 19 S antibody was detectable on either day 7 or day 14. The data are consistent with the theory that there is a sequential synthesis of the two antibodies. The lysin which predominates in the serum on day 14 was identified as a γSS-globulin by immunoelectrophoresis. The early lysin may be either a γ1A-globulin or a γSS-globulin.