Sarcoma-180 Tumor Cells Research Articles

Influence of dimethyl sulphoxide on glycine transport in sarcoma 180 tumour cells.

DIMETHYL sulphoxide (DMSO) inhibits a number of biosynthetic processes; growth of fibroblasts1 and bacteria2, DNA synthesis in sarcoma-180 tumour cells3, thyroidal transport and organification of iodine (our unpublished observations), and protein systhesis4,5 are all inhibited by sufficiently high concentrations of this compound in vitro. The activity of a number of enzymes6 is also reduced in similar concentrations of DMSO. An interesting fact is that these inhibitory effects of DMSO are reversible—they can be overcome by a sufficient reduction in the DMSO concentration, for example, by “washing” the tissue, cells or enzyme in physiological saline. The mechanism of DMSO inhibition, which is unknown, may involve non-specific enzyme inhibition, although the activity of some enzymes is increased in DMSO6.

Sequential Synthesis of Two 7 S Lysins by Rats Challenged with Mouse Sarcoma-180 Tumor Cells

Summary The administration of S-180 mouse ascites tumor cells to rats has been shown to stimulate the production of two distinct lysins capable of rupturing the tumor cells in vitro. The two antibodies were separated on diethylaminoethyl (DEAE)-cellulose and were shown by sucrose density gradient centrifugation and by chromatography on Sephadex G-200 to be 7 S antibodies. No 19 S antibody was detectable on either day 7 or day 14. The data are consistent with the theory that there is a sequential synthesis of the two antibodies. The lysin which predominates in the serum on day 14 was identified as a γSS-globulin by immunoelectrophoresis. The early lysin may be either a γ1A-globulin or a γSS-globulin.