Sappan Research Articles

Investigating the potential of waste oyster shell as a sustainable bio-mordant in natural dyeing

The use of natural bioresources in textile dyeing has attracted significant research interest due to their environmentally friendly and low-toxic nature. This study investigated the utilization of waste oyster shell (WOS) as a bio-mordant combined with sappan wood extract to dye silk fabrics using pre-, meta-, and post-mordanting method. The crystal structure of CaCO3 derived from WOS and their impact on color properties, color strength and color fastness were analyzed. The dyed fabrics displayed a range of shades from light pink to deep red and brown. FTIR analysis confirmed chemical interactions among dye, silk, and WOS mordants. Notably, silk treated with WOS at 800 °C as a pre-mordant showed color strength approximately 2.6 times higher than that of un-mordanted samples. Additionally, bio-mordanted samples exhibited improved color fastness compared to untreated ones (rating 2), with pre-mordanting offering the highest resistance to washing (rating 4–5). These findings shed light on the potential of waste oyster shell as an effective, sustainable alternative to conventional metal mordants in textile dyeing. This study not only support the utilization of waste but also enhances environmental and resource efficiency of the dyeing process.

Brazilin Content, Color Stability, and Antioxidant Activity of Sappan Wood (Caesalpinia Sappan L.) Traditional Drink by Different Blanching and Drying Methods

Caesalpinia sappan L (CS) heartwood is used as a natural red colorant in several Asian ethnic traditional drinks both as sole ingredient or mixed with other herbs. However, the pigment is unstable and easily degraded during storage which affects consumer acceptance. This research aimed to investigate the effect of different blanching and drying methods on the color stability, brazilin content, and antioxidant activity of CS heartwood drinks. Steam blanching, water blanching, sun drying and cabinet drying were examined in this study. The most common method applied in traditional production, without blanching and sun drying, was used as a control. The experimental design used was complete randomized design with triplicates. Data are presented as mean ± standard deviation and analyzed by SPSS statistics version 20. The results showed that blanching and drying methods affected the color intensity. For a pure CS heartwood herbal drink, the sample prepared with water blanching and cabinet drying (BCD) showed the highest red value, leading to a 48 % improvement compared to the control. This red value is in accordance with the brazilin content. Sample BCD showed the highest brazilin content, which was 376 % higher than that of the control. Similarly, the BCD sample had the highest antioxidant activity and total phenolic content, with IC50 values of 98.99 ppm and 213.12 mg GAE g–1, respectively. This result revealed that brazilin might act as an antioxidant. Similar to the pure CS drink, the highest quality of mixed herbal drink was obtained by BCD sample. The results suggest that the combination of water blanching and cabinet drying (BCD) was the best method to improves the quality of CS heartwood drink both in pure and mixed herbal drinks as a functional drink. HIGHLIGHTS Samples treated with blanching and drying had higher brazilin content of Sappan heartwood drink There is a correlation between brazilin content and color intensity as well as antioxidant activity Blanching and cabinet drying improved the color stability of Sappan heartwood drink Blanching and cabinet drying increased the total phenolic and flavonoid content of Sappan heartwood drink GRAPHICAL ABSTRACT

Plant-mediated synthesis of biocompatible ZnO NPs: comprehensive characterisation and investigation of their catalytic, corrosion inhibition, antibacterial, scavenging and anti-oncogenic activities

ABSTRACT In this study, we present a novel method for synthesising zinc oxide nanoparticles (ZnO NPs) from the dried Caesalpinia sappan seeds powder. Comprehensive characterisation using XRD, FTIR, SEM, and EDX techniques was employed to elucidate the surface and morphological properties of these ZnO NPs. We showcase the versatile application of ZnO NPs as heterogeneous catalyst in the synthesis of 5-arylmethylidene-pyrimidin-2,4,6-triones. Notably, this eco-friendly approach eliminates the need for organic solvents, offering a sustainable route to producing 5-arylmethylidene-pyrimidin-2,4,6-triones in high yields. Furthermore, we investigate the corrosion inhibition properties of ZnO NPs on mild steel in 5% sodium chloride solution. Our findings reveal the ZnO NPs’ significant inhibition efficiency against corrosion, promising potential for practical applications. In addition to their chemical applications, we delve into the biological activities of ZnO NPs, including their antibacterial effects against both Gram-positive and Gram-negative bacteria, anti-oncogenic activity against DU-145 cells, and scavenging activities against oxidative stress.

Influence of microstructure and tribology properties on deep-dipping coating method of sappanwood (SW)/ polyvinyl alcohol (PVA)/ Polyethylene (PE)/ Aluminium nitride (AlN) natural fiber nanocomposite materials

Influence of microstructure and tribology properties on deep-dipping coating method of sappanwood (SW)/ polyvinyl alcohol (PVA)/ Polyethylene (PE)/ Aluminium nitride (AlN) natural fiber nanocomposite materials

Iron chelating, antioxidant, and anti-inflammatory properties of brazilin from Caesalpinia sappan Linn

BackgroundIron overload and inflammation are severe conditions that can lead to various chronic diseases. However, the current iron chelator drugs have their limitations. The phytochemical compounds from herbals, such as brazilin, the major active compound in Caesalpinia sappan Linn., have significant therapeutic potential in various chronic diseases. Our study was designed to examine the effect of brazilin on iron chelating properties, antioxidant activity in hepatocytes, and anti-inflammatory potential in macrophages. MethodsThis study focused on the isolation, purification, and evaluation of brazilin, the principal bioactive constituent found in C. sappan wood. Brazilin was extracted via methanol maceration followed by column chromatography purification. The purified compound was characterized using high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry (MS). The antioxidant potential of brazilin was assessed by in vitro assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazolin-6-sulfonic acid (ABTS), and ferric-reducing antioxidant power (FRAP). Furthermore, its cellular antioxidant activity was evaluated using hydrogen peroxide-induced oxidative stress in the hepatocellular carcinoma cell line (Huh-7). The iron-chelating capacity of brazilin was determined spectrophotometrically, and Job's plot method was used to elucidated the stoichiometry of the iron-brazilin complex formation. The anti-inflammatory properties of brazilin were investigated in lipopolysaccharide (LPS)-stimulated macrophages (RAW 264.7). Nitric oxide (NO) inhibition was quantified using the Griess reagent, while the expression levels of pro-inflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), were evaluated by RT-qPCR. ResultsThe results demonstrated that brazilin exhibited potent antioxidant activity in vitro and hepatocytes in a concentration-dependent manner. It also showed anti-inflammatory activity, in which NO production was significantly reduced and IL-6 and TNF-α expression in LPS-induced macrophages were repressed. Furthermore, it can bind ferric and ferrous ions. Brazilin acts as a bidentate iron chelator that forms a complex with iron in a 2:1 ratio, and two water molecules are used as additional chelators in this complex. ConclusionsOur findings have significant implications. Brazilin can potentially alleviate the harmful effects of iron-induced oxidative stress and inflammatory disorders.

The application of copper sulfate mixed with Caesalpinia sappan L. extract to increase colorfastness in pressed blush products

This study aims to increase the colorfastness of pressed blush products by combining copper sulfate with Caesalpinia sappan L. extract. This experiment consisted of four conditions used for color extraction: 70% w w-1, 95% w w-1, 70% w w-1 with 10% w w-1 copper sulfate, and 95% w w-1 with 10% w w-1 copper sulfate. The highest yield percentage of crude extract was found at 70% w w-1, with the highest absorption value (λmax) at 70% methanol with 10% w w-1 copper sulfate. The darkest shade of red was found at 70% w w-1 with 10% w w-1 copper sulfate. The development of pressed blush occurred when corn starch was mixed with more white color and smell than arrowroot flour. The highest red color was achieved at 4.5% w w-1, with the highest red - purple intensity. The microbial contamination test showed no microbial contamination in pressed blush products. GRAPHICAL ABSTRACT HIGHLIGHTS Caesalpinia sappan L. was extracted using maceration methods with methanol solvent. Phytochemical screening revealed pink anthraquinone and anthocyanidins. The chemical properties showed a pH range of 5.31–5.74, maximum light absorption, and mordant increased absorption. A compress blush was developed with corn starch and arrowroot flour, suitable for the cosmetic industry.

Respons pertumbuhan tanaman secang (Caesalpinia sappan L.) terhadap dosis pupuk kandang sapi

Sappan wood (Caesalpinia sappan L.) is a plant that contains the compound brazilin, which is found in the pith of its stem. The pith of the sappan wood stem can be used as a raw material for traditional medicine or as a dye with red pigment. Currently, there are no procedures or guidelines for cultivating techniques for sappan wood plants. Each annual plant requires a certain amount of organic material at the beginning of planting to support its growth. The organic material commonly used is manure, one of which is cow manure. This research aims to determine the effect of cow manure dosage on the growth of sappan plants. The experiment was conducted from March 2023 to December 2023 at Ciparanje Research Center, Faculty of Agriculture, Universitas Padjadjaran. The method used is experimental by design Randomized Block Design consisting of three treatments namely, it was repeated six times: 1 kg cow manure/plant, 2 kg cow manure/plant, and 3 kg cow manure/plant. The experimental results showed that treatment with 2 kg cow manure/plant had the best effect on plant height at 5,6,7,8, and 9 months after treatment (MAT) and the number of leaves at 5 and 9 MAT. There has been no response in plant diameter to the dose of cow manure fertilizer

MOLECULAR DOCKING STUDIES OF FLAVONOIDS FROM SECANG WOOD (Caesalpinia sappan L.) AGAINST GLUCOKINASE ENZYME AS ANTIDIABETIC CANDIDATES

Diabetes mellitus (DM) is a metabolic disease caused by a deficiency of insulin secretion, insulin resistance, and increased hepatic glucose production. Secang wood (Caesalpinia sappan L.) is known to have antihyperglycemic activity. However, these compounds are not yet known. In silico studies are needed to determine the compounds that act as antidiabetics. This study performed molecular docking of flavonoid compounds in sappan wood against the 1V4S glucokinase receptor. The results showed that all flavonoid compounds of sappan wood were predicted to have antidiabetic activity because they had a lower docking score than metformin, the first-line therapy of type 2 diabetes mellitus. Butein is expected to have the best activity. It has the lowest docking score (-94.4836). Visualization of the docking results shows that butein interacts with the identical amino acid residues as metformin, namely ARG 63 and THR 65, through the formation of hydrogen bonds and Van der Waals interactions. SWISS-ADME web tool predicted that butein has good oral absorption and excretion. The toxicity prediction tool showed a slight contradiction in the mutagenic effect. Based on this research, molecular docking may be able to design new drugs, especially from butein in sappan wood (Caesalpinia sappan L.), as antidiabetic candidates.

Sappan wood extraction for intelligent gelatin packaging films: a review on implementing green packaging solutions

Sappan wood extraction for intelligent gelatin packaging films: a review on implementing green packaging solutions

Molecular Docking of Brazilin from Secang Wood Plant (Caesalpinia sappan L.) as an Anti-Breast Cancer

Breast cancer is a disease characterized by abnormal cell growth, which can invade adjacent tissues or metastasize to other organs. Secang (Caesalpinia sappan L.) is a plant that has been used as an alternative medicine for a variety of health conditions, including some types of cancer. This study aims to determine whether the brazilin compound found in secang wood can interact with the target receptors estrogen alpha, 17-β-HSD-1, and NUDT5, potentially serving as an anticancer candidate. Molecular docking simulations were employed to identify the molecular interactions of brazilin against estrogen receptor alpha (ERα) (PDB ID: 3ERT), 17β-HSD-1 (PDB ID: 3HB5), and NUDT5 (PDB ID: 5NQR) using AutoDockTools software. The results showed that the best free-binding energy (ΔG) value obtained for brazilin against the 17-β-HSD-1 receptor was -9.16 kcal/mol, with an inhibition constant of 192.45 nM. The ΔG value of brazilin with estrogen alpha was -6.68 kcal/mol, and the ΔG value for brazilin against NUDT5 was -4.8 kcal/mol. Brazilin has a higher potency compared to innate ligands based on the docking result of estrogen alpha receptor and NUDT5. Some structural similarities and interactions occurred between the amino acids GLY186 and TYR155 in brazilin with the binding of amino acids formed in the innate ligand against the 17-β-HSD-1 receptor, thus showing similar affinity to the 17-β-HSD1 receptor. In silico approaches provided valuable insights into the potential of brazilin as an anti-breast cancer agent.

Antioxidant, antiglycation, and antibacterial of copper oxide nanoparticles synthesized using Caesalpinia Sappan extract.

Synthesis of metal nanoparticles using plant extracts is environmentally friendly and of increasing interest. However, not all plant extracts can meet successfully on the synthesis. Therefore, searching for the high potential extracts that can reduce the metal salt precursor in the synthesis reaction is essential. The present study explores the synthesis of copper oxide nanoparticles (CuONPs) using Caesalpinia sappan heartwood extract. Phytochemical analysis and determination of the total phenolic content of the extract were performed before use as a reducing agent. Under the suitable synthesized condition, a color change in the color of the solutions to brown confirmed the formation of CuONPs. The obtained CuONPs were confirmed using ultraviolet-visible spectroscopy, photon correlation spectroscopy, X-ray diffraction, scanning electron microscope, energy dispersive X-ray, and Fourier transform infrared analysis. The synthesized CuONPs investigated for antioxidant, antiglycation, and antibacterial activities. CuONPs possessed antioxidant activities by quenching free radicals with an IC50 value of 63.35 µg/mL and reducing activity with an EC range of 3.19-10.27 mM/mg. CuONPs also inhibited the formation of advanced glycation end products in the bovine serum albumin/ribose model with an IC50 value of 17.05 µg/mL. In addition, CuONPs showed inhibition of human pathogens, including Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, and prevention of biofilm formation and biofilm eradication, with maximum inhibition of approx. 75%. Our findings suggest that C. sappan extract can be used to obtain highly bioactive CuONPs for the development of certain medical devices and therapeutic agents.

Effect of Sappan Wood Ethanol Extract in CRP Level and Phagocytic Index Between Group of Mice Infected with S. aureus and E. coli

S.aureusand E.coli are pathogenic bacterial that cause many infectious disease in the world. Immunomodulator is needed to prepare the immune system to be able against the infection. Some parameters usually used to assess the immunomodulatory activity such as C-Reactive Protein (CRP) and phagocytic index. This study aims to investigate the difference effect of sappan1wood extract in CRP level and phagocytic index between group mice infected with S. aureus and E. coli. Two treatment groups of mice were prepared for S. aureus and E. coli test. Each group subjected to 7 treatments i.e. (1) Normal mice1(CMC-Na 1 %- Merck® 217277), 1(2) Negative Control (bacterial infection + CMC-Na 1 %),11(3) Positive Control (bacterial infection + Imboost force®1treatment, PT SOHO Industri Pharmasi), (4) Bacterial infection and EESW treatment 25 mg/kg BW, (5) 50 mg/kg BW, (6) 100 mg/kg BW, and (7) 200 mg/kg BW. Mice blood was taken to detect the CRP and phagocytic index after treatment. The T test showed that there was a significant difference between CRP levels (p0.05) and phagocytic index (p0.05) of S. aureus and E. coli group. EESW 200 mg/kg BW reduced CRP level to 11 mg/dL (S. aureus) and 6 mg/dL in (E. coli). EESW 200 mg/kg BW increased phagocytosis to 1.54 folds (S. aureus) and 4.62 folds (E. coli). Sappan wood ethanol extract effect to CRP level and phagocytic index1in mice group infected with E. coli is better than S. aureus1infection group.

In Silico Study of the Potential of Brazilein Sappan Wood as a Beta-Lactamase Inhibitor against Extended-Spectrum Beta-Lactamase-Encoding Genes.

Infectious illnesses are a serious health concern in Indonesia. Widespread use of self-medication by the community increases the risk of developing multi-drug resistant (MDR) bacteria. This study assessed the potential of sappan wood as an inhibitor of extended-spectrum beta-lactamase (ESBL) encoded by blaSHV, blaTEM and blaCTX-M genes. In silico testing was conducted to develop an effective and economical starting strategy. Thereby, this study significantly advances the development of novel treatments to combat antibiotic resistance. Using clavulanic acid as the benchmark medicine, the potency of the beta-lactamase inhibitor brazilein was predicted. Using the Molegro Virtual Docker computer tool, docking was performed to estimate the chemical and physical properties of the compounds, as well as the biological activity of brazilein toward the required receptor. The receptors used were SHV-1 beta-lactamase, PDB code: 2H0T; TEM-1 beta-lactamase, PDB code: 4OQG and CTX-M-14 beta-lactamase, PDB code: 6VHS. Data analysis was performed by comparing the binding energies of the docking results between the ligands and the target receptor. The more stable the bond that formed between the ligand and the target receptor, the lower the bond energy. The in silico test results on the blaSHV gene were as follows: binding energy of ligand MA4_400[A] = -100.699, brazilein = -82.206, clavulanic acid = -79.3704; in the blaTEM gene: ligand bond energy 2UL_301[B] = -107.681, brazilein = -82.0296, clavulanic acid = -103.3; in the blaCTX-M gene: X57_301[A] ligand bond energy = -86.6197, and brazilein = -88.1586, clavulanic acid = -101.933. The findings of this study demonstrate the significant potential of brazilein sappan wood to block the beta-lactamase activity of blaCTX-M.

Pharmacognostical Studies on the Leaves of Caesalpinia sappan Linn (Fabaceae)

Botanical medicine, herbalism, and herbal medicine are all terms that describe the same practice. There are many different chemical compounds found in and produced by herb plants that have physiological effects. Traditional herbal medicine is an integral part of the medical systems of all indigenous peoples, including Ayurvedic, naturopathic, homeopathic, traditionally oriental, and native American Indian medicines. The amount and quality of medicinal components determine the medicinal plant’s therapeutic effectiveness. Misidentification is the first step in the unlawful use of plant-based materials or herbal remedies. Pharmacognostical studies of medicinal plants by proper standardization parameters ensures plant identification and authentication. Brazil, or Sappan wood, is a popular name for Caesalpinia sappan Linn., which belongs to the Fabaceae family. In Hindi, it is referred to as Bakam/Patang. The Malay Peninsula, Sri Lanka, India, Myanmar, and Vietnam all have a long history of using the medicinal herb C. sappan Linn. heartwood is beneficial for Pitta, burning feelings, injuries, ulcers, leprosy, dermatological ailments, dysentery, diabetes, loose stool, and more, according to Ayurveda. The tree’s huge, decorative panicles of yellow blossoms are the reason it’s grown in gardens. The plant grows rapidly after being produced from seed. Cultivated throughout every region of tropical Asia, this spreading shrub or tree may reach a height of 10 m. It is native to and exists in the wild in places like the southern part of India, the West Bengal region, Orissa, Madhya Pradesh, Malaya, as well as Sri Lanka. The wood has an especially smooth, uniform appearance, is somewhat massive, and has a strong, orange-red color. Its grain is straight. Branchlets, pubescent and rufous, equipped with tiny prickles. Pinnae are 9 to 14 pairs in number. Leaves are big, hairy to glabrous, and have little prickles at the base and leaflets are subsessile, oblong, membranous, and obliquely truncate, with 10 to 20 pairs per pinna. Primarily, it promotes blood flow and acts as an analgesic, emmenagogue, hemostatic, and anti-inflammatory in traditional Chinese medicine for traumatic diseases. One of the ingredients of the world-famous Indian toothpaste and powder Vicco-Vajradanti is the wood of the C. sappan Linn Tree. Some research suggests that C. sappan Linn. may have antidiabetic effects. The flavonoid of C. sappan Linn. wood extract is protosappanin, Brazilin, and gallic acid compounds. Brazilin has a mechanism of action to increase the production of fructose 2,6 bisphosphate and hexose six phosphate. Fructose 2,6 bisphosphate in the metabolic process has a role in regulating glycolysis and gluconeogenesis in the liver. Increased fructose 2,6 bisphosphate in a state of hyperglycemia will stimulate the process of glucose breakdown (glycolysis) by activating phosphofructokinase 1 intended for the purpose of can be a reduction in blood glucose levels in body. This paper deals with the morphological studies and microscopical studies carried out on the leaves of C. sappan Linn according to the World Health Organization (WHO) accepted parameter for the identification of medicinal plants. For a better understanding of structure, zooming in using a Zeiss Axio Cam Erc5s digital camera and a Nikon ECLIPSE-E200 trinocular microscope under strong field lighting also employed.

Analysis of Metoxyl and Galacturonate Contents from Pectin of Banana Peel Waste Combined with Sappan Wood

The production of banana plants (Musaceaea sp.) ranks first in agricultural output in Indonesia, but this is not balanced by the processing of waste from banana peels, which is a large amount. Therefore, an attempt is made to use banana peel waste as raw material for making pectin. Pectin is used as a functional component in the food industry because of its ability to form thin gels and stabilize proteins. Pectin is also used as a filler in the paper and textile industries, and as a thickener in the rubber industry. This research aims to determine the effect of extraction time and temperature on the quality of pectin from Kepok banana peels. The method used is extraction through Ultrasonic Assisted Solvent Extraction (UASE) in 40 kHz. Banana peels that had been dried and ground into powder were extracted with 0.1 N HCl at temperatures of 70 oC, 80 oC and 90 oC. The variables used in this extraction are variations in extraction time, namely 60 minutes and 80 minutes. The extracted solution is filtered and thickened to half the volume of the original filtrate by heating, then the pectin is thickened using acid ethanol. The pectin precipitate was washed using 96% alcohol until it was free of chloride and the precipitate was separated using a vacuum and then dried in an oven then to analysis of pectin using methoxyl and galacturonate as method to determine pectin quality form banana peel waste. The research results showed that the pectin quality was optimum at a temperature of 90 oC for 80 minutes with a methoxyl content of 4.12% (low methoxyl) and a galacturonic acid content of 79.96%.

Potensi Antibakteri Minuman Fungsional Tradisional Jawa (Wedang Uwuh) Berdasarkan Variasi Waktu Rebusan

Wedang uwuh is a functional beverage prepared by boiling a combination of spices, namely sappan wood, ginger, nutmeg leaves, cinnamon leaves, cloves, and clove leaves. It is reported to have antioxidant, antibacterial, and immune-enhancing properties due to its active compounds. This traditional beverage shows potential in reducing infections caused by Escherichia coli, highlighting the importance of proper preparation methods. The objective of this study was to evaluate the antibacterial activity of wedang uwuh prepared by different boiling duration (5, 10, 15, and 20 min) to determine the optimal time for maximum effectiveness. Phytochemical changes, as indicated by color alterations, were assessed by a descriptive approach while a disc diffusion method was used as an antibacterial assay to measure the inhibition zone diameter against E. coli. Compounds in each treatment were analyzed using GC-MS. Phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, triterpenoids, and tannins in all variations. Antibacterial testing revealed that boiling for 15 min exhibited the highest activity, with an inhibition zone diameter of 10.43±1.33 mm, reflecting strong antibacterial efficacy. This result significantly differed from the 5 and 10 min treatments. The 5 min boiling treatment demonstrated a smaller inhibition zone with an average diameter of 5.71±1.67 mm, indicating moderate antibacterial activity, but it did not differ significantly from the 10 min treatment. A chemical analysis by GC-MS for the 15 min boiling sample successfully identified 23 compounds, and among these chemicals, hexadecanoic acid was found as the predominant component.

Effect of Foam Mat Drying on Antioxidants in Secang Wood Extract (Caesalpinia Sappan L)

There is a notable increase in interest in utilizing herbal plants, with some herbal products being particularly rich in bioactive compounds, including antioxidants. Antioxidants play a pivotal role in the protection of the body against oxidative stress, through the neutralization of harmful free radicals. One plant extract that exhibits notable antioxidant activity is sappan wood extract (Caesalpinia sappan L). The objective of this study is to assess the impact of foam formulation and temperature on the drying process and antioxidant activity of sappan wood extract, which has been dried by the foam-mat drying method. The research was conducted in three stages, namely extraction, drying and analysis of results. Drying of sappan wood extract was carried out at 40, 60 and 80°C, with different compositions of foaming agent. The results demonstrated that antioxidant activity exhibited a tendency towards stability at a drying temperature of 40°C. However, the highest reduction in water content was observed with drying at 80°C. The most effective foam composition was identified as foam composition 2, 0% CMC + 20% egg white (albumin) + 10%. Conversely, the foam composition with the highest antioxidant activity was identified as foam composition 5, 0.5% CMC + 30% gum Arabic. The most appropriate antioxidant degradation equation for this study was the first-order equation. The most accurate drying rate model for this study was the Lewis drying rate model.

Research of the Dyeing Properties of Lychee Leaves and Sappan Wood Extract on Silk-Linen Fabric

Synthetic dyes are the primary colouring materials for textile dyeing; however, the residual industrial wastewater from the dyeing process contains complex concentrations of chemical substances, which result in toxicity and environmental pollution with severe negative impacts on ecology and health. This study adopts an eco-friendly perspective, utilizing a mixture of lychee leaves and sappan wood extracts for dyeing silk-linen fabrics. It explores the impacts of dyeing time, mordants, and dyeing methods on the colour quality of the samples. A computer colourimeter was used to compare the colour effects of different dye solution ratios on silk-linen fabrics, and the colour fastness to water, perspiration, and dry rubbing of the dyed fabrics was tested. The results show that the optimized dyeing process for silk-linen fabrics using a mixed solution of lychee leaves and sappan wood includes the following findings: Dyeing time has no significant impact on the CLE L*, a*, and b* values. The highest L* value and colour brightness are achieved under conditions without mordants. By mixing dye solutions in different proportions and treating them with mordants, the colour gamut of silk-linen fabrics has been expanded. The dyed colours are similar to the main popular colours of 2024, making them the trend colours of this year; the samples' fastness performance tests indicate that the colour fastness to water, perspiration, and dry rubbing essentially meets the requirements for textile fabrics. The use of lychee leaves and sappan wood for fabric dyeing is not only environmentally friendly but also possesses significant health-beneficial properties, offering potential commercial value.

THE EFFECTIVENESS OF SAPPAN WOOD EXTRACT (Caesalpinia sappan L.) AS AN ALTERNATIVE MATERIAL FOR DENTAL PLAQUE IDENTIFICATION

The main factor of periodontal disease is dental plaque, which is attached to the surface of the teeth and consists of microorganisms originating from saliva and food debris. Dental plaque has a transparent colour, so a disclosing agent is needed to identify it. Sappan wood (Caesalpinia sappan L.) is a plant that contains brazilin and flavonoids that can be used for natural colouring. This study aims to determine the effectiveness of sappan wood extract (Caesalpinia sappan L.) as an alternative material for dental plaque identification. The experiment was conducted by measuring the plaque index of 20 people who did not clean their teeth for 12 hours for two periods. The first period of examination used a tablet disclosing agent, and the second period used 75% sappan wood extract. The research data were analyzed for normality with the Shapiro-Wilk dan Paired T-test. The result showed a significant difference in the mean plaque index score in the first period (1.3470) and the second period (1.0375) (p<0.05). It was concluded that staining dental plaque using a disclosing agent is more effective than 75% sappan wood extract (Caesalpinia sappan L.)

Caesalpinia sappan Reduce Fever with An Anti-inflammatory and Antioxidant Mechanism: A Review

<p><em><span lang="EN-US">Caesalpinia sappan L</span></em><span lang="EN-US"> (Sappan wood) is an herbal plant that has long been trusted by the public as an herbal medicine for tuberculosis, diarrhea, dysentery, skin infections, anemia, and other diseases by utilizing the decoction of <em>C.sappan</em>. Sappan wood is an herbal plant widely used as a raw material for traditional medicinal products. Sappan wood has been reported to have substantial pharmacological effects in analgesic, antioxidant, antibacterial, anti-inflammatory, and anti-viral. Fever is a clinical manifestation of certain conditions or diseases characterized by increased body temperature above the normal range (36.5–37.5 °C). Many studies declare that antioxidant and anti-inflammatory activity in <em>C.sappan</em> reduces oxidative stress and pro-inflammatory cytokines. This literature review shows that the antioxidant and anti-inflammatory properties of <em>C.sappan</em> are linked to fever as a sign of illness. Literature review using the last ten years' Scopus, ScienceDirect, and Pubmed databases. There are as many as 20 journals regarding sappan wood's antioxidant and anti-inflammatory effects. Sappan wood has been shown to have an antioxidant effect by lowering reactive oxygen species levels via SOD, GPx, or CAT markers. It inhibits inflammation cytokines such as IL-1, IL-6, TNF-</span><span lang="EN-US">𝝰</span><span lang="EN-US">, and INF produced during fever. Sappan wood also has an anti-inflammatory effect by inhibiting PGE2 production when someone has a fever. The findings of our review state that <em>C.sappan</em> can be used to treat fever for both of these reasons. The use of <em>C.sappan</em> as a component in producing traditional health beverages has potential.</span></p>