Abstract: 
 
 Sapindus trifoliatus L. is an extremely valuable medicinal plant distributed in tropical and subtropical regions of Asia. Plant extracts appear to be one of the better alternatives as they are known to have a minimal environmental impact and danger to consume in contrast to synthetic pesticides. The seed extracts of Sapindus trifoliatus L. exhibited activity against Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Pseudomonas aeruginosa. The antimicrobial activity of each plant extract was tested against bacterial and fungal organisms
 I.e. Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and
 E.coli. Aspergillus niger, Aspergillus flavous and Fusarium spp.
 The antimicrobial activity was done for Chloroform, Ethyl Alcohol, Petroleum Ether, Acetone and Aqueous extracts. The study revealed that the ethanolic extract of the pericarp of Sapindus trifoliatus L. showed positive prominent antibacterial activity against E.coli and Staphylococcus aureus. The ethanol extract showed antifungal activity against Aspergillus fumigates and Aspergillus niger at 100% plant extract. The inhibitory activity of the various seed extracts like Chloroform, Acetone, Petroleum ether, Ethyl alcohol and aqueous were observed.