Osteoarthritis is a joint deformity, often found in progressive degeneration of the articular cartilage, resulting in loss of joint space. Genu osteoarthritis patients complain of pain, limited movement, and a decrease in their functional activities. Patients with severe complaints use mobility aids for walking activities. This research uses a quasiexperimental design with one group pre and post test design approach. The sample for this research was 30 respondents. The sampling technique for this research is purposive sampling, which is a sample selection technique by selecting samples among a number of populations that are in accordance with what the researcher desires. There is an effect of quadriceps strengthening exercise on increasing muscle strength and reducing pain intensity in osteoarthritis genu patients at Bhayangkara Kediri Hospital in 2023. The average muscle strength of genu osteoarthritis patients before quadriceps strengthening exercise was 3.533 and the average pain intensity of genu osteoarthritis patients before quadriceps strengthening exercise was 4.8. The average muscle strength of genu osteoarthritis patients after quadriceps strengthening exercise was 4.6 and the average pain intensity of genu osteoarthritis patients after quadriceps strengthening exercise was 3.05. There is an effect of quadriceps strengthening exercise on increasing muscle strength with P-value 0,000<0,05 and effect of quadriceps strengthening exercise on reducing pain intensity in osteoarthritis genu patients signification p value 0,000<0,05.