Homogeneous Sample Research Articles

High-harmonic spin-shearing interferometry for spatially resolved EUV magneto-optical spectroscopy

We present a method for achieving hyperspectral magnetic imaging in the extreme ultraviolet (EUV) region based on high-harmonic generation (HHG). By interfering two mutually coherent orthogonally-polarized and laterally-sheared HHG sources, we create an EUV illumination beam with spatially-dependent ellipticity. By placing a magnetic sample in the beamline and sweeping the relative time delay between the two sources, we record a spatially resolved interferogram that is sensitive to the EUV magnetic circular dichroism of the sample. This image contains the spatially-resolved magneto-optical response of the sample at each harmonic order, and can be used to measure the magnetic properties of spatially inhomogeneous magnetic samples.

The Chinese version of the general benefit finding scale (GBFS): Psychometric properties in a sample of college students.

Benefit finding has become a central construct in the evolution of positive psychology and attracted attention in recent literature. This study aimed to translate and validate the General Benefit Finding Scale (GBFS) in Chinese college students. Forward- and back-translation of the GBFS was followed by the assessment of semantic equivalence and content validity. A sample of 589 college students was recruited in China to conduct reliability and validity analysis. The construct validity was assessed using exploratory (EFA) and confirmatory factor analysis (CFA). Concurrent validity was assessed using Pearson's correlation coefficients of the GBFS with the Perceived Stress Scale (PSS) and World Health Organization-Five Well-Being (WHO-5). Internal consistency and two-week test-retest reliability were also evaluated. The content validity index for each item ranged from 0.83 to 1.00. EFA revealed a six-factor model, which exhibited acceptable goodness of fit in CFA (standardized root mean square residual = 0.031, root mean square error of approximation = 0.059, goodness-of-fit index = 0.860, comparative fit index = 0.904, Tucker-Lewis index = 0.890, chi-squared/degree of freedom = 2.07). The concurrent validity of the GBFS was supported by its statistically significant correlations with PSS (r = -0.271, p<0.001) and WHO-5 (r = 0.354, p<0.001). Moreover, the internal consistency for the overall scale was satisfactory, with Cronbach's α coefficient of 0.93 and McDonald's omega reliability of 0.94. The test-retest reliability was 0.82. Although the Chinese version of GBFS was examined in a homogeneous convenience sample of college students, it provides a reliable and valid instrument for assessing benefit finding in the Chinese context.

A green and facile direct ink writing technique for preparation calibration standards in laser ablation inductively coupled plasma mass spectrometry analysis

BackgroundLaser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful tool for microanalysis of solid materials. Nevertheless, one limitation of the method is the lack of well-characterized homogeneous reference materials (RMs), such as BaF2 crystal and BaCO3 ceramics samples, making direct quantification difficult. This work presents a novel Direct Ink Writing (DIW) method to produce RMs for microanalysis. The Mg, Cr, Fe, Co, Ni, Cu, Y, Mo, Pr, Gd, Dy, Ho, Er, Tm, Yb, and Lu solutions were gravimetrically doped into BaCO3 by mixing with the dispersant and then cured with DIW techniques. (94) ResultsBaCO3 powder was combined with a dopant analyte to produce a printable slurry, aided by the use of a dispersant and cellulose. The resulting mixture was then printed using DIW equipment. The retention rates of the doped elements were investigated by internal and external standard method, and the results showed that they were completely dispersed in the solid material. After further optimization, it was found that there was no significant heterogeneity among the printed samples. LA-ICP-MS was used to analyze printed samples, to evaluate micro-scale homogeneity. The mass concentration of the doped element was determined by ICP-MS, verify its move closer to nominal value. Compared with the traditional reference materials preparation methods, the DIW technology greatly increased the sample homogeneity and the accuracy of the desired concentration. (132) SignificanceAs far as we know, there are few reports on the application of DIW method to prepare calibration standards. In brief, it is proved that the proposed method of preparing calibration standard by DIW technique to quantify analytes is valid and robust. This procedure provides great potential for LA-ICP-MS in-situ analysis in the field of well-prepared products, such as ceramic and crystal samples.(63)

Non-destructive characterization of silicon nanowires and nanowire coatings using mid-infrared spectroscopy

Silicon nanowires (SiNWs) have attracted much attention owing to their potential applications in electronics and photonics, while remaining as a cost-effective material platform. Key material properties in engineering SiNWs for different applications include their length, density, and presence of oxides or other chemicals on the surface. However, monitoring these properties is challenging, as scanning electron microscopy and atomic force microscopy (AFM), which directly image the wires, require destructive cleaving of the sample. This paper uses polarized, variable-angle Fourier Transform IR reflectance spectroscopy as a nondestructive technique to characterize the areal density and length of metal-assisted chemically etched SiNW arrays. In addition to the ability to characterize “as-etched” wires, we show that IR spectroscopy can also measure few-nanometer thick layers of aluminum oxide (AlOx) grown radially around the length of the SiNWs utilizing atomic layer deposition. Despite sample inhomogeneities, an effective medium theory (EMT) model can determine the length and density of the NWs for a range of lengths between 3 and 14 μm. The EMT can also reproduce the experimentally measured vibrational bands for coated NWs, demonstrating that IR spectroscopy can also evaluate the presence of molecular contaminants on the wires.

How long can you store vitamins? Stability of tocopherols and tocotrienol during different storage conditions in broccoli and blueberries

Differences between the stability of α-, β-, γ-, and δ-tocopherol as well α-tocotrienol stored at −20 °C and −80 °C were studied in broccoli and blueberry samples. Before storage up to 28 days, they underwent different initializing processes such as freezing quickly with liquid nitrogen and freeze-drying, followed by homogenization. While α-tocopherol levels in blueberries did not significantly differ, levels in broccoli were substantially higher after homogenization of freeze-dried samples compared to fresh broccoli samples. This might be caused by higher extractability of α-tocopherol from the changed cell structure. Storage of fresh broccoli samples at −20 °C led to decreasing α-tocopherol levels. Nevertheless, the deviation between freeze-dried samples to the initial fresh samples and fresh samples frozen with liquid nitrogen stored at −20 °C for 7 days were in the same order of magnitude. In conclusion, storage up to 7 days for vitamin relevant samples before analysis seemed to be justifiable.

Autism Sports Education: Transforming Lives of Families

It is very important to integrate individuals with autism into social life in order to minimize the effects of autism, which is a developmental problem that lasts for a long time and needs to be lived together and is increasing numerically every day. In this context, 14 parents of individuals with autism participated in the research conducted to determine the effect of the Sports Education Project for People with Autism (SEPA), which was carried out in cooperation with Gazi University, on the daily lives of autistic individuals and their families. In the research, the phenomenology design, which is among the qualitative research methods, was utilized. Using homogeneous sampling technique, focus group interviews were conducted with 14 parents who had been attending the SEPA for 4 months without interruption. In general, the participants found the impact of SEPA on their daily lives to be positive; in terms of parents, they stated that it allowed them to share problems with other parents and increase solidarity, thus allowing them to socialize, contributed positively to family interactions and made them feel happier. In terms of their children, they stated that they contributed positively to their physical and social development and that this situation facilitated their children's daily lives. The findings obtained support that projects such as the Children with Autism Education Project will contribute positively to the life processes of individuals with autism and their families. It is recommended that studies in this direction be expanded.

Evidence of SARS-CoV-2 Antibody in Mississippi White-Tailed Deer.

Background: Early detection and monitoring of SARS-CoV-2 infections in animal populations living in close proximity to humans is crucial for preventing reverse zoonosis of new viral strains. Evidence accumulated has revealed widespread SARS-CoV-2 infection among white-tailed deer (WTD), (Odocoileus virginianus) populations in the United States except in the southeast region. Therefore, the objective was to conduct surveillance for evidence of SARS-CoV-2 infection among WTD in Mississippi. Materials and Methods: Blood, kidney tissues, and nasal swab samples were collected in 17 counties from hunter-harvested deer during 2021-2022 and 2022-2023.Samples of kidney tissue were collected to evaluate for detecting antibody as a possible alternative to blood that is not always available from dead WTD. Nasal swab samples were tested for SARS-CoV-2 viral RNA by a RT-PCR assay. Sera and kidney tissue samples were tested for SARS-CoV-2 antibody by an enzyme-linked immunoassay (ELISA) and sera by a plaque reduction neutralization test (PRNT80). Results: The results of testing sera and kidney homogenate samples provided the first evidence of SARS-CoV-2 infection among WTD in Mississippi. The infection rate during 2021-2022 was 67% (10/15) based on the detection of neutralizing antibody by the PRNT80 and 26%(16/62) based on the testing of kidney tissue homogenates by an ELISA, and viral RNA was detected in 25% (3/12) of nasal swab samples. In 2022 to 2023, neutralizing antibody was detected in 62% (28/45) of WTD serum samples. In contrast, antibodies were not detected in 220 kidney homogenates by an ELISA nor was viral RNA detected in 220 nasal swab samples. Evidence of WTD activity was common in urban areas during the survey. Conclusion: Overall, the findings documented the first SARS-CoV-2 infection among WTD in Mississippi and showed that WTD commonly inhabited urban areas as a possible source of acquiring infection from humans infected with this virus.

POTENTIAL IMPACT OF EXERCISE UPON SYMPTOM BURDEN IN ADOLESCENTS AND YOUNG ADULTS UNDERGOING CANCER TREATMENT

INTRODUCTION Adolescents and young adults (AYAs) experience vast symptom burden resulting from cancer treatment-related toxicities (TRTs). Evidence supports exercise to mitigate several TRTs in other cohorts, however evidence in AYAs is lacking. Conventional reporting of TRTs adopts a maximum grade approach failing to recognise the trajectory over time or lower grade toxicities. Alternatively, longitudinal analysis of toxicities over time (ToxT) may provide clinically meaningful summaries of this data. We evaluated the longitudinal impact of an exercise intervention on TRTs in AYAs undergoing cancer treatment. METHODS A prospective, randomised trial allocated participants to a 10-week exercise intervention (EG) or control group (CG) undergoing usual care. Detailed information on TRTs was collected throughout the intervention. All TRTs were graded per the Common Terminology Criteria for Adverse Events (CTCAE v5.0). RESULTS Forty-three (43) participants (63% male, mean age 21.1 years) were enrolled. When categorised to reflect the maximal worst grade experienced (Grade 0, Grade 1-2 and ≥Grade 3), the CG reported an increased incidence of severe fatigue (≥Grade 3) compared with the EG (p=0.05). No other differences between groups were evident (p&amp;gt;0.05). ToxT analysis of the four most common toxicities (fatigue, pain, nausea and mood disturbances) demonstrated no difference in the mean grade of each over time (p&amp;gt;0.05). Additionally, area under the curve (AUC) analysis revealed trends towards a higher magnitude of fatigue (mean AUC 12.5 vs 11.1 p=0.11) and mood disturbances (mean AUC 7.2 vs 5.7 p=0.28) over time in the CG. No differences were evident for pain and nausea between groups (p&amp;gt;0.05). CONCLUSION A 10-week exercise intervention reduces the severity of fatigue in AYAs undergoing treatment. While the ToxT approach provided insight into the toxicity profile, adequately powered studies are needed to better understand these differences within a homogeneous sample

PENGARUH PENGGUNAAN MEDIA PEMBELAJARAN DAN GAYA KOGNITIF TERHADAP HASIL BELAJAR MATEMATIKA SISWA KELAS VIII MADRASAH TSANAWIYAH

This is a quasi-experimental study (Quasi-Experimental Design) nonequivalent control group design with applying 3 × 2 factorial design. Subjects were students MTs Laboratory Fak. Tarbiyah IAIN STS Jambi City class VIII semester of academic year 2012/2013. Data collection was conducted by using two different instruments namely instruments Group Embedded Figures Test (GEFT) to measure cognitive styles. Instrument in the form of students and students' mathematics achievement test form of objective questions (multiple choice). Hypotheses were tested using analysis of Varian Two Line (Two-Way ANOVA), after testing prerequisites are met ie the data are normally distributed and the variance between relatively homogeneous sample. From the data analysis and statistical test results obtained are: (1) there is the influence of media use on learning outcomes of mathematics teaching eighth grade students of MTs, (2) there is an influence of cognitive style on learning outcomes of students' mathematics class VIII MTs (and (3) there is no significant interactions between media learning and cognitive style on learning outcomes of students' mathematics class VIII MTs Keyword: interactive cd, powerpoint, konvensional, cognitive style and learning outcomes

Differences in medically assisted reproduction use by sexual identity and partnership: a prospective cohort of cisgender women.

Differences in medically assisted reproduction use by sexual identity and partnership: a prospective cohort of cisgender women.

A speech fluency brain network derived from gliomas.

The brain network of speech fluency has not yet been investigated via a study with a large and homogenous sample. This study analysed multimodal imaging data from 115 patients with low-grade glioma to explore the brain network of speech fluency. We applied voxel-based lesion-symptom mapping to identify domain-specific regions and white matter pathways associated with speech fluency. Direct cortical stimulation validated the domain-specific regions intra-operatively. We then performed connectivity-behaviour analysis with the aim of identifying connections that significantly correlated with speech fluency. Voxel-based lesion-symptom mapping analysis showed that damage to domain-specific regions (the middle frontal gyrus, the precentral gyrus, the orbital part of inferior frontal gyrus and the insula) and white matter pathways (corticospinal fasciculus, internal capsule, arcuate fasciculus, uncinate fasciculus, frontal aslant tract) are associated with reduced speech fluency. Furthermore, we identified connections emanating from these domain-specific regions that exhibited significant correlations with speech fluency. These findings illuminate the interaction between domain-specific regions and 17 domain-general regions-encompassing the superior frontal gyrus, middle frontal gyrus, inferior frontal gyrus and rolandic operculum, superior temporal gyrus, temporal pole, inferior temporal pole, middle cingulate gyrus, supramarginal gyrus, fusiform gyrus, inferior parietal lobe, as well as subcortical structures such as thalamus-implicating their collective role in supporting fluent speech. Our detailed mapping of the speech fluency network offers a strategic foundation for clinicians to safeguard language function during the surgical intervention for brain tumours.

Anxiety among youth with food allergy.

Anxiety is among the major psychological concerns for children living with food allergy (FA). Yet research exploring the variables driving anxiety symptoms in FA remains sparse, and most studies still utilize homogeneous samples to assess anxiety symptoms. The current study seeks to evaluate the rates of clinically significant anxiety symptoms among a diverse sample of youth with FA and examine whether a heightened risk perception of FA outcomes and FA burden (vs. FA medical history) is associated with anxiety in youth. 94 youth ages 10-14 and their parents were recruited from FA clinics at a mid-Atlantic children's hospital. Both youth and parents completed demographic and FA medical history questionnaires, the Screen for Child Anxiety Related Emotional Disorders, and the Food Allergy Independent Measure as part of a longitudinal study about FA adjustment and adherence. Over a third (37%) of youth scored above clinical cut-offs for overall anxiety symptoms. At least 25% of youth reported clinically significant scores on panic disorder, generalized anxiety, social anxiety, separation anxiety, and school avoidance subscales. Perception of risk of adverse FA outcomes and burden-but not FA medical history-were associated with total anxiety, generalized anxiety, panic disorder, and school avoidance symptoms, but not social anxiety and separation anxiety. Having more FAs was associated with higher social anxiety scores but not with other anxiety subscales. Youth with FA might benefit from psychosocial interventions that address FA risk perception management and promote appropriate FA vigilance to cope with anxiety symptoms.

Insights into the theory-practice gap: Perspectives from South African industrial psychologists

Orientation: Emerging industrial psychologists enter into an internship after completion of their studies. Interns sometimes lack the required competencies, which may be a result of a gap between theory taught at university and expected competencies in practice.Research purpose: The general objective of this study was to explore the beliefs of supervising industrial psychologists regarding the theory-practice gap, and the perceived competence of graduates entering industrial psychology internships.Motivation for the study: The existence of the theory-practice gap in industrial psychology in South Africa is largely unexplored. Understanding the current level of competence and identifying the aforementioned gaps, universities and interns can proactively reduce the perceived gap.Research approach/design and method: A qualitative descriptive research design was employed. Data were collected through qualitative surveys among a homogenous sample of 13 industrial psychologists supervising interns. Content analysis was used to analyse the data.Main findings: Interns display some competencies, while lacking others. The majority of the participants confirmed a gap between theory acquired at university and industry. The participants made recommendations to universities and interns to close this gap.Practical/managerial implications: Identifying a theory-practice gap and the impact it may have on the competence level of emerging professionals, students and universities can adapt their learning and teaching strategies to potentially help minimise the gap to ensure graduates are more competent when entering internships.Contribution/value-add: This research study adds qualitative insights on the topic of the theory-practice gap, specifically the perceptions of supervising industrial psychologists within South African organisations.

Preparation of Binary and Ternary Laves and µ-Phases in the Ta–Fe(–Al) System for Property Analysis at the Microscale

Topologically close-packed (TCP) intermetallic phases are attractive candidates for adapting the property profile of both structural and functional materials, but their mechanical behavior, in particular below their brittle-to-ductile transition, is generally still poorly understood. The systematic analysis of the underlying deformation mechanisms requires the fabrication of homogeneous samples with sufficiently high purity and large enough grain size. Here, we describe identified pathways on the example of Laves and µ-phases from the binary Ta–Fe and ternary Ta–Fe–Al systems with regard to input materials, liquid metallurgy synthesis, heat treatment, and metallographic preparation methods. Preparation routes for structural analysis by electron backscatter diffraction and mechanical analysis by nanomechanical testing, as well as the transferability of our approach to other alloy systems containing TCP phases, are outlined and discussed.

Disparities in Mortality by Sexual Orientation in a Large, Prospective Cohort of Female Nurses

Extensive evidence documents health disparities for lesbian, gay, and bisexual (LGB) women, including worse physical, mental, and behavioral health than heterosexual women. These factors have been linked to premature mortality, yet few studies have investigated premature mortality disparities among LGB women and whether they differ by lesbian or bisexual identity. To examine differences in mortality by sexual orientation. This prospective cohort study examined differences in time to mortality across sexual orientation, adjusting for birth cohort. Participants were female nurses born between 1945 and 1964, initially recruited in the US in 1989 for the Nurses' Health Study II, and followed up through April 2022. Sexual orientation (lesbian, bisexual, or heterosexual) assessed in 1995. Time to all-cause mortality from assessment of exposure analyzed using accelerated failure time models. Among 116 149 eligible participants, 90 833 (78%) had valid sexual orientation data. Of these 90 833 participants, 89 821 (98.9%) identified as heterosexual, 694 (0.8%) identified as lesbian, and 318 (0.4%) identified as bisexual. Of the 4227 deaths reported, the majority were among heterosexual participants (n = 4146; cumulative mortality of 4.6%), followed by lesbian participants (n = 49; cumulative mortality of 7.0%) and bisexual participants (n = 32; cumulative mortality of 10.1%). Compared with heterosexual participants, LGB participants had earlier mortality (adjusted acceleration factor, 0.74 [95% CI, 0.64-0.84]). These differences were greatest among bisexual participants (adjusted acceleration factor, 0.63 [95% CI, 0.51-0.78]) followed by lesbian participants (adjusted acceleration factor, 0.80 [95% CI, 0.68-0.95]). In an otherwise largely homogeneous sample of female nurses, participants identifying as lesbian or bisexual had markedly earlier mortality during the study period compared with heterosexual women. These differences in mortality timing highlight the urgency of addressing modifiable risks and upstream social forces that propagate and perpetuate disparities.

Reference-free x-ray fluorescence analysis with a micrometer-sized incident beam

Spatially resolved x-ray fluorescence (XRF) based analysis employing incident beam sizes in the low micrometer range (μXRF) is widely used to study lateral composition changes of various types of microstructured samples. However, up to now the quantitative analysis of such experimental datasets could only be realized employing adequate calibration or reference specimen. In this work, we extent the applicability of the so-called reference-free XRF approach to enable reference-free μXRF analysis. Here, no calibration specimen are needed in order to derive a quantitative and position sensitive composition of the sample of interest. The necessary instrumental steps to realize reference-free μXRF are explained and a validation of ref.-free μXRF against ref.-free standard XRF is performed employing laterally homogeneous samples. Finally, an application example from semiconductor research is shown, where the lateral sample features require the usage of ref.-free μXRF for quantitative analysis.

Walking an extra mile: Determinants of organizational citizenship behavior—An exploratory study in faith‐based organizations

AbstractObjectiveOur study is intended to explore the factors that promote organizational citizenship behaviors (OCB) in faith‐based organizations, which has the potential to extend the scholarly conversation around a previously unexplored context.MethodsOur study used a purposive, homogeneous sampling technique in selecting the participants. We interviewed 30 employees who have at least 5 years of experience in faith‐based organizations. We conducted detailed interviews and subsequent analysis involved a rigorous six‐step thematic analysis process to better understand the phenomena being studied.ResultsOur study revealed the significance of OCB in faith‐based organizations and further elucidated those factors that determine the display of OCBs. Our findings have the power to enhance the existing comprehension of OCBs in different environments, specifically those present in faith‐based organizations. Our study broadens our conceptual understanding of OCB in faith‐based organizations by adding one more dimension to the existing framework of P. MPodsakoff et al. (1990).ConclusionOur findings have important implications for organizational scholars in faith‐based organizations. Our new conceptual framework offers insights into the distinct characteristics of OCBs in faith‐based organizations and suggests directions for future scholars to engage with OCBs from different contexts.

CLI: A new protocol for the isolation of Lactic Acid Bacteria from complex plant samples

Lactic Acid Bacteria (LAB) are predominantly probiotic microorganisms and the most are Generally Recognized As Safe (GRAS). LAB inhabit in the human gut ecosystem and are largely found in fermented foods and silage. In the last decades, LAB have also has been found in plant microbiota as a new class of microbes with probiotic activity to plants. For this reason, today the scientific interest in the study and isolation of LAB for agronomic application has increased. However, isolation protocols from complex samples such as plant tissues are scarce and inefficient.In this study, we developed a new protocol (CLI, Complex samples LAB Isolation) which yields purified LAB from plants. The sensitivity of CLI protocol was sufficient to isolate representative microorganisms of LAB genera (i.e. Leuconostoc, Lactococcus and Enterococcus).CLI protocol consists on five steps: i) sample preparation and pre-incubation in 1% sterile peptone at 30 °C for 24–48 h; ii) Sample homogenization in vortex by 10 min; iii) sample serial dilution in quarter-strength Ringer solution, iv) incubation in MRS agar plates with 0.2% of sorbic acid, with 1% of CaCO3, O2 < 15%, at pH 5.8 and 37 °C for 48 h.; v) Selection of single colonies with LAB morphology and CaCO3-solubilization halo. Our scientific contribution is that CLI protocol could be used for several complex samples and represents a useful method for further studies involving native LAB.

Impact of Modified Frailty Index on Readmissions Following Surgery for NSCLC.

Analyzing the risk factors that predict readmissions can potentially lead to more individualized patient care. The 11-factor modified frailty index is a valuable tool for predicting postoperative outcomes following surgery. The objective of this study is to determine whether the frailty index can effectively predict readmissions within 90 days after lung resection surgery in cancer patients within a single health care institution. Patients who underwent elective pulmonary resection for nonsmall cell lung cancer (NSCLC) between January 2012 and December 2020 were selected from the hospital's database. Patients who were readmitted after surgery were compared to those who were not, based on their data. Propensity score matching was employed to enhance sample homogeneity, and further analyses were conducted on this newly balanced sample. A total of 439 patients, with an age range of 68 to 77 and a mean age of 72, were identified. Among them, 55 patients (12.5%) experienced unplanned readmissions within 90 days, with an average hospital stay of 29.4 days. Respiratory failure, pneumonia, and cardiac issues accounted for approximately 67% of these readmissions. After propensity score matching, it was evident that frail patients had a significantly higher risk of readmission. Additionally, frail patients had a higher incidence of postoperative complications and exhibited poorer survival outcomes with statistical significance. The 11-item modified frailty index is a reliable predictor of readmissions following pulmonary resection in NSCLC patients. Furthermore, it is significantly associated with both survival and postoperative complications.

Development and Validation of Novel HPLC Methods for Quantitative Determination of Vitamin D3 in Tablet Dosage Form.

In the present work, an efficient isocratic HPLC method was developed for the precise and accurate estimation of vitamin D3 in tablet form. The chromatographic conditions comprised an L3 silica column (5 µm in particle size, 4.6 mm × 250 mm) with a mobile phase n-hexane/ethyl acetate (85:15 v/v) with a flow rate of 2.0 mL/min and a detection wavelength of 292 nm. The new methodology was validated for accuracy, precision, specificity, robustness, and quantification limits according to an official monograph of USP/BP and ICH guidelines. The peak areas of the six replicates of the homogeneous sample were recorded. The mean value obtained was 67,301, and the relative standard deviation (RSD) was 0.1741. The linearity and range were in the acceptable bounds, i.e., 0.999, which was calculated using regression line analysis. The results show that the method is truly acceptable as the RSD, as the flow rate was 0.81%, while for the mobile phase composition, it was 0.72%, which lies in the acceptable range. The limit of detection (LOD) and the limit of quantification (LOQ) values were 0.0539 µg/mL and 0.1633 µg/mL, respectively. The % RSD of the intra and inter-day precision of the method was deemed acceptable according to the international commission for harmonization guidelines. The developed method has potential to be used for the detection and quantification of vitamin D3 during routine analysis for tablets in dosage form.