Fish Eggs Research Articles

Estimated Hydraulic Characteristics and the Entrainment of Fish Eggs and Larvae at a Pumped River‐Water Intake

AbstractWithdrawal of fish eggs and larvae through a river intake (entrainment) may damage the river's early fish resources. To investigate how the hydraulics (flow velocities, directions, and magnitudes) around and within the water intake structure influence entrainment, this study focused on a typical river‐pump intake. A turbulence model was developed based on the Euler‐Lagrange method and the variable of helicity was introduced to define the zone of the river from which water is withdrawn and organisms are entrained. The process of simulated hydraulics on organism withdrawal was validated by physical experiments using artificial fish eggs under various river flow and intake flow conditions. The simulated results indicated that when the intake‐to‐river flow ratio ranged from 3 to 7‰, the width of the planar entrainment area was approximately 1.2 to 1.4 times the width of the intake structure, and the entrainment quantity of fish eggs and larvae accounted for 0.12% to 0.49% of the incoming flux. The entrainment quantity increased with the intake flow to the river flow ratio. The absolute helicity threshold under different conditions ranged from 0.004 to 0.047 m/s2, which was inversely proportional to the intake flow rate and unaffected by river flow and water depth. An optimized intake structure design with stepped side walls was promoted, which can minimize the impact of river sedimentation and reduce the fish entrainment quantity by an average of 11%. This research provides valuable insights for water intake safety and fish resource protection.

Decreases in pH from effluent had a devastating but reversible impact on the coastal plankton communities

An event of releasing untreated effluent caused serious decreases in surface seawater pH from 8.1 to lower than 7.5 in seven years and increased back to prior levels after 15 years. It gives us a rare natural experiment to examine the impacts of decreases in pH on the marine plankton communities (phytoplanktons, zooplanktons, shrimp larvae, crab larvae, fish eggs, and larvae) in the natural environment. Observed decreases in pH had a nonlinear effect ubiquitous on all plankton groups, leading to a reduction of approximately 50 % in their density and abundance compared to the level at pH 8.1. Non-linear responses of planktons implied the existence of specific groups more robust to decreases in pH. As pH bounced back to normal levels, the density and abundance of the plankton communities also recovered, further indicating that the negative impacts of decreases in pH on the marine plankton communities were reversible.

Hormonal symphony: The dynamic duo of IGF and EGF in gonadotropin-induced fish ovarian development and egg maturation.

Fish oocyte maturation (FOM) is a critical biological process that occurs before ovulation and is influenced by gonadotropins, particularly luteinizing hormone (LH). The release of LH stimulates the ovarian follicle to produce a maturation-inducing hormone (MIH), specifically 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-DP), which initiates the formation of maturation-promoting factor (MPF) through the activation of cyclin B and cdc2 kinase. Insulin-like growth factor I (IGF-I) significantly regulates ovarian functions, including steroidogenesis, by activating its membrane receptors and the tyrosine kinase pathway. IGF-I influences oocyte maturation directly via the PI3 kinase pathway, independent of steroid hormones. Additionally, epidermal growth factor (EGF) promotes cell growth and differentiation by binding to its receptor (EGFR). It is implicated in mediating human chorionic gonadotropin (hCG)-induced DNA synthesis in ovarian follicles while suppressing apoptosis. The presence of EGF in follicle cells and oocytes, along with its higher expression in oocytes, suggests it may act as a paracrine signal regulating somatic cell activity. Recent studies indicate that the activin system in follicle cells could be a target for EGF activity. The EGFR signaling pathway enhances gonadotropin-induced steroidogenesis and governs the transition of oocyte maturation stages, essential for successful fertilization. This review synthesizes current research on the roles of gonadotropins, IGFs, and EGFs in fish oocyte maturation and ovarian steroid production.

Effect of artificial fish nest placement on spawning site preference and reproductive efficiency in reservoir fish with adhesive and demersal eggs

Artificial fish nests are common tools in fisheries management, providing spawning grounds to enhance the size and diversity of fish populations. This study aimed to explore the effects of deployment locations on the reproductive efficiency and preferences of fish with adhesive and demersal eggs using artificial nests. Floating artificial nests were deployed in three regions (upstream, midstream, and downstream) of a reservoir in Zhejiang, China, at locations with three topographical types: steep slope (reservoir shore, slopes > 60°), gentle slope (reservoir shore, slopes < 30°), and confluence (middle thread of channel). The quantity, viability and species of eggs in artificial nests were recorded throughout the fish reproductive cycle. The mean density of viable eggs in artificial nests in the midstream region (1176 ± 448 ind./m2) was significantly higher than that in the downstream (477 ± 167 ind./m2) and upstream regions (350 ± 148 ind./m2). The survival rate of fish eggs in the gentle slope area (56.94 ± 11.97%) was significantly lower than that in the confluence (79.80 ± 6.20%) and steep slope areas (74.47 ± 11.40%). Different fish groups respond uniquely to environmental factors. We suggest that the construction and implementation of artificial nests should be based on the differences in upstream and downstream water bodies, as well as the specific ecological requirements of target fish species. Management practices should focus on site-specific environmental monitoring and adapt nest placement to optimize conditions for different species. Furthermore, future research should aim to develop predictive models that integrate environmental factors and species needs, providing a more robust basis for artificial nest placement and fisheries management.

Detection of Tilapia parvovirus in farm-reared tilapia in India and its isolation using fish cell lines.

Tilapia parvovirus (TiPV) is an emerging viral pathogen and responsible for severe economic loss in tilapia culture production. Lethargic, cutaneous haemorrhages; ocular lesions; discolouration of gill and cloudy eye and exophthalmia are common symptoms of TiPV. The TiPV-suspected tilapia fish were collected from grow-out ponds situated in different parts of Tamil Nadu, India, and screened for TiPV by PCR. The results showed the presence of TiPV in disease-suspected fish which was further confirmed by PCR using different primer sets specific to different genomic regions of TiPV. Sequence analysis of 534bp of genomic region of TiPV showed 100% similarity with the sequence of TiPV strain of Thailand and India. TiPV was found in different organs including eggs of infected fish and showed the possibility of systemic infection and vertical transmission. Snakehead kidney (CSK), snubnose pompano fin (SPF) and tilapia heart (TH) cell lines showed susceptibility to TiPV. The viral replication in cell lines was confirmed by PCR, TiPV-specific cytopathic effect of Cowdry A inclusion bodies with clear halo surrounding them and infectivity experiment. The disease was reproduced in normal fish by intramuscular route using viral inoculum from TiPV-infected fish or virus multiplied in susceptible cell lines to satisfy Koch's postulates.

Physicochemical characteristics and metabolite composition of fish sauce made from large yellow croaker roe enzymatic hydrolysates based on three fermentation temperatures.

Large yellow croaker roes were used to make fish sauce using enzymolysis method, and the impact of different fermentation temperatures (25, 32 and 40°C) on the physicochemical characteristics and metabolite composition was revealed. Results indicated that compared with 25 and 32°C, the higher contents of total acidity, amino nitrogen, total soluble nitrogen, and the deepening of the nonenzymatic browning degree of fish sauce fermented at 40°C were obtained. The total volatile basic nitrogen content at 40°C was lower than 32°C. The ideal fermentation temperature should be 40°C. The metabolomics analysis of fish sauce showed that 40°C significantly increased the abundance of hippuric acid, 3-hydroxy hippuric acid, hydrocinnamic acid and heptanoic acid compared with other temperatures, but decreased trimethylamine N-oxide, 15-methyl palmitic acid, N6-succinyl adenosine and nicotinamide. These results expand the utilization pathways of low-value fish roes, and provide a promising reference for the manufacturing of high-quality fish sauce.

Effect of Thiamine and Its Metabolites on Survival of Danio rerio Prelarvae under Different Salinity

The paper deals with the effect of thiamine and its metabolites thiamine disulfide, thiochrome and 4-methyl-5-&amp;beta;-hydroxyethylthiazole on survival of &lt;i&gt;Danio rerio&lt;/i&gt; prelarvae under the different salinity. The absence of the thiamine and its metabolites' effect on the fish eggs' development under the salinity range of 0-3&amp;#8240; was established. The survival rate of prelarvae decreased along with salinity growth, and under thiamine and its derivatives it decreased even more, but the effect of metabolites was individual.

Gerontological Impact on Embryonic Development of Common Carp Cyprinus Carpio Var. Communis

The present study deals with the gerontological impact on embryonic development of hatchery-based broodstock of Cyprinus carpio var. communis (Linnaeus, 1758) in two different age groups (0 and +1 year). The average size of fertilized eggs (adhesive, round and yellowish) of the experimental fish was recorded as 1.00±0.04 and 1.15±0.06 mm for 0 (t0) and +1year (t1) age groups, respectively. About eight hours post-fertilization (hpf), the gastrula disc began to protrude gradually and formed the germinal ring in fertilized eggs of the +1-year brooder group while the development of eggs of 0-year group was comparatively slow. The head and tail region development became visible at 16 hpf in the +1-year group eggs. Head region became visible in the 0-year group embryos at 30 hrs post-fertilization while in the +1-year group optic primordium and somites developed prominently at this stage. Hatching took place 52 hpf in +1 year while in the other group, it occurred at 54 hpf. The yolk sac was absorbed rapidly in the hatchlings of +1-year group compared to 0-year group while after the yolk sac absorption, development was observed almost to be the same in both groups` embryos. This embryological study is advantageous in choosing better broodstock for aquaculture production.

DNA Barcodes for Identifying Fish Egg Species Diversity in Summer and Autumn in the Southwest Daya Bay, China

Identifying fish eggs and understanding fish reproductive periods are necessary for informed fishery management. However, accurate the identification of fish eggs is difficult because eggs have few distinct characters, and their morphology varies ontogenetically. Using cytochrome c oxidase subunit I, we identified fish eggs from ichthyoplankton samples collected in the summer and autumn of 2021 from southwestern Daya Bay, China. Of 567 fish eggs, 498 high-quality cytochrome c oxidase subunit I sequences were obtained, of which 116 eggs (23.3%) could be identified to species; 364 (73.1%) to genus, family and/or order; and 18 (3.6%) could not be assigned. Of 51 apparent taxa, 46 were identified to 6 orders, 19 families, and 30 genera; 20 to the species and 25 to the genus and/or family, and 1 to the order. Among these 51 taxa, 35 occurred in summer, 29 occurred in autumn, and 13 occurred in both seasons; 22 occurred only in summer and 16 only in autumn, indicating species-specific spawning periods. High-resolution photographs of eggs are provided to facilitate subsequent identification based on morphology. These results will facilitate the identification of spawning grounds and their protection, to more holistically manage fishery resources in Daya Bay, China.

Chemical Warfare in the Environment-Secondary Metabolites From Starfish (Asterias amurensis) Induce Teratogenicity in Medaka Embryos (Oryzias melastigma).

Starfish saponins, known for their role as feeding deterrents against predators like crabs and fish, have been extensively studied for their antifeedant and cytolytic effects. Recent research suggests that starfish secondary metabolites possess antifouling and antifeedant properties and play a role in biological interactions and various ecological functions. Previous research demonstrated that saponins from starfish exert their toxic effects on fish by interacting with cholesterol in the blood cell membrane. This study investigated the hypothesis that secondary metabolites, other than saponins, from the starfish Asterias amurensis may be harmful to fish eggs, particularly marine medaka (Oryzias melastigma) eggs, which share starfish habitat. We systematically separated the secondary metabolites of A. amurensis by a variety of chromatographic methods. Two oligoglycosides related to embryonic teratogenicity were identified, including one previously undescribed tetrasaccharide (1) and one known pyrrole oligoglycoside (2). Their structures were established mainly on the basis of detailed analysis of the nuclear magnetic resonance spectroscopy (NMR) and mass spectroscopic data. Compounds 1 and 2 exhibited strong lethality and significantly reduced voluntary movements in medaka embryos, with compound 2 showing more pronounced effects on teratogenicity and heart rates. The main morphological abnormalities observed included delayed head development (DHD), tiny spines (TS), incomplete absorption of oil balls (OB), cardiac abnormalities (CA), and shrunken yolk sacs (SYS). Our findings imply that starfish secondary metabolites may have broader ecological effects, influencing habitat-sharing species in subtle but crucial ways.

Spinal deformity in a whale shark, Rhincodon typus (Smith 1828), encountered in the northern Gulf of Mexico, with notes on its movement patterns.

This note details the first formal report of a spinal deformation in whale sharks, Rhincodon typus. An individual whale shark with suspected kypholordoscoliosis was observed at Ewing Bank in the Gulf of Mexico during aggregation events in 2010 and 2013. Despite the significant deformity, the shark was observed feeding on fish eggs at the surface during both encounters. Based on satellite tag tracking, its movements, temperature preferences, and depth use were within the range of other whale sharks from the region.

Density and diversity of larval fish in some coastal bays in Sharm El-Sheikh, northern Red Sea, Egypt

This study was carried out to investigate the composition and the spatial and temporal distribution of coral reef fish larvae in Port Bay, Sharm El- Maya Bay and Naama bay located in Sharm El-Sheikh area on the Egyptian Red Sea coast. Ichthyoplankton samples were collected monthly for one year mostly in the morning just before and at sunrise. A net of 100 cm, and 0.5 mm mesh size was towed horizontally parallel to the reef for 5–10 min. Throughout the period of study, a total of 205 plankton samples resulted in a collection of 3678 larvae representing 46 taxa and 32 fish families. Although larvae occurred all the year round, the general pattern showed two peaks in summer and winter with their maximum density in August with 1030 individuals/1000 m3. The lowest density of fish larvae was observed in September with 12 individuals/1000 m3. Regarding the distribution in bays, Port Bay (PRT) recorded the highest larval density where 1476 individuals/1000 m3 were collected. On the other hand, the lowest density was recorded in Sharm El-Maya Bay (SMB) with an average density of 908 larvae/1000 m3. The highest density of fish larvae occurred in any sample was recorded in Naama bay in August with a density of 1824 individuals/1000 m3. In several cases the spawning seasons of these taxa as determined from the present work based on the seasonal occurrence of larvae coincides mostly with that obtained from the literature based on the reproductive biological studies. Results obtained during this work reflected the importance of fish eggs and larvae survey in the management and conservation of reef fish in the Red Sea.

Investigating the Presence of Selenoneine, Ergothioneine, and Selenium-Containing Biomolecules in Fish and Fish-Derived Commercial Products.

Two potent antioxidants, selenoneine and ergothioneine, have been determined simultaneously for the first time in the muscle of highly consumed fish and in fish-derived products. The extraction methods were optimized, and their subsequent analysis was performed by HPLC-ESI-MS/MS. Best extraction efficiency (106 ± 3 and 104 ± 5%) was attained by performing a simple aqueous extraction containing 100 mmol L-1 ammonium acetate or ammonium formate as extractants. Selenoneine and ergothioneine were simultaneously detected in tuna (0.306 ± 0.006 and 3.5 ± 0.2 μg g-1) and swordfish (0.14 ± 0.02 and 1.1 ± 0.4 μg g-1) muscle tissues, while in fish roe and in farmed salmon, only ergothioneine was detected (with contents ranging 0.03-3.3 μg g-1). Moreover, the presence of their respective methylated forms was also considered. Additionally, Se-containing biomolecule profiles from water-soluble proteins were obtained by SEC-ICP-MS analysis. Results revealed similar profiles between fish and fish roe, whereas for ultraprocessed fish products, Se seems to be mostly associated with low-molecular-weight biomolecules. The results included in this work may contribute to a better understanding of the relations between selenoneine and ergothioneine in fishery products as well as their fate and potential modifications of Se-containing biomolecule profiles during fish processing.

DNA barcode reference library of the fish larvae and eggs of the South China Sea: taxonomic effectiveness and geographic structure

Fish early-stages constitute useful indicators of the states of marine ecosystems, as well as important fishery resources. Given the spectacular phenotypic changes during ontogeny, and the paucity of diagnostic morphological characters at the species level, the identification of fish early-stages is a challenging task. DNA barcoding, the use of the mitochondrial gene of the cytochrome c oxidase subunit I (COI) as an internal species tag, opened new perspectives for the identifications of both larval fish and fish eggs. However, the accuracy of the identifications assisted by DNA barcoding are dependent of the completeness of the DNA barcode reference libraries used to assigned unknown sequences to known species. Here, we built a DNA barcode reference library for 113 species of larval fish and 85 species of fish eggs involving the production of 741 newly generated DNA barcodes from South China Sea (63 localities). Together with 514 DNA barcodes mined from Genbank for 116 species from the South China Sea regions, a reference library including 1255 DNA barcodes for 308 species (248 locations) was assembled. The present study emphasizes the importance of integrating DNA barcoding to large scale inventories of early stages, as DNA-based species delimitation analyses delimited 305 molecular operational taxonomic units (MOTUs) and multiple cases of discordance with morphological identifications were detected. Cryptic diversity is detected with 14 species displaying two MOTUs and a total of 23 species were lumped into 11 MOTUs due to low interspecific divergence and/or mixed lineages.

Latitudinal variation in zooplankton over the Emperor Seamounts (34°–44° N, 170°–171° E) during the summer of 2019

The horizontal and vertical distributions of zooplankton over the Emperor Seamounts (Koko, Jingu, Nintoku, and Suiko guyots) were analyzed. The zooplankton was represented by four genera of amphipods, seven genera of pteropods, 39 genera of copepods, and larvae of benthic invertebrates and fish. The abundance and biomass of zooplankton increased towards higher latitudes along the series Koko < Jingu < Nintoku < Suiko guyots. The highest index of diversity was recorded over the Koko and Jingu guyots; the highest species richness occurred over the Jingu and Nintoku guyots. Small-sized copepods, appendicularians, chaetognaths, euphausiids, and fish eggs and larvae concentrated in the epipelagic; large copepods and ostracods were concentrated in the mesopelagic. We identified three types of zooplankton assemblages: a Subtropical assemblage between 34°–35°N characterized by tropical/subtropical forms; a Transition assemblage characterized by subtropical, subarctic, and widespread species between 38°–41°N; and a Subarctic assemblage characterized by subarctic and widespread species between 43°–44°N. The spatial distribution of zooplankton was a function of environmental variables such as surface salinity, temperature at 200 m, and, to a certain extent, by surface Chl-a concentrations.

Effect of Microplastic Exposure to the Reproductive Energy and Fecundity of Female Wami Tilapia (Oreochromis urolepis, Norman 1922) Fish

ABSTRACTThere is mounting evidence indicating that microplastics (MPs, &lt; 5 mm) cause reproductive dysfunction in fish, yet information on the long‐term effects of MP exposure remains scarce. In this study, Wami tilapia fries were exposed to 38–45 µm polyethylene (PE) MPs for their first 2 months in treatment groups of control (0 PE MPs/mL), 1, 10, and 100 PE MPs/mL (with 60 individuals per group in triplicates), and subsequently maintained in a ratio of 3 females to 1 male for an additional 5 months. Reproductive proxies and parameters of female fish health were calculated, and models were developed according to the Akaike information criterion (AIC) and no significant differences in fecundity, relative fecundity, hepatosomatic index (HSI), weight, length, gonadosomatic index, and oocyte packing density were found between treatment groups (one‐Way ANOVA, p &gt; 0.05). However, condition factors varied significantly between treatment groups (Kruskal–Wallis Test, p = 0.006), with the control group differing significantly from the groups exposed to 1 PE MPs/mL (p = 0.007), 10 PE MPs/mL (p = 0.03), and 100 PE MPs/mL (p = 0.001). Fecundity showed strong correlations with weight and length in all treatment groups except the group exposed to 10 PE MPs/mL (weight: r = 0.471, p = 0.346; length: r = 0.688, p = 0.131) and showed insignificant correlations with condition factors and HSI. Multiple regression models revealed that weight significantly contributed to fecundity in all treatment groups except the group exposed to 10 PE MPs/mL. Histopathological analysis indicated damage to livers and small intestines proportional to the dose of PE MPs. This study demonstrates that long‐term exposure of fish to MPs has no significant effect on fecundity but impairs fish health, which could potentially jeopardize the quality of fish eggs and recruitment.

Declines in coastal macrozooplankton communities within a recent climate and ocean tipping point in the Tropical South Atlantic

This study investigated whether variations in climate and ocean drivers on global, regional, and local scales affected macrozooplankton communities in a coastal protected area in Tamandaré Bay (northeastern Brazil). For this purpose, bimonthly field campaigns were carried out from June 2013 to August 2019. A significant tipping point (point of change, p < 0.001), with an abrupt increase in SST, was detected in the TSA (Tropical South Atlantic) index. This indicates the existence of a climate regime shift in the Tropical South Atlantic during the 2015/16 El Niño (EN) event. Extreme rainfall events were observed in Tamandaré Bay after this EN event, in 2017, 2018, and in 2019 (and more recently, in 2022). This extreme rainfall led to low-salinity events, increased variability in salinity, and significantly lower abundances in the period after the strong EN event, for socioeconomically relevant penaeid shrimp postlarvae and several other zooplankton groups (e.g., copepods, appendicularians, anomuran hermit crab larvae, and chaetognaths). We found a significant relationship between SSTs in the TSA region and penaeid shrimp recruitment in the study area, located leewards of the TSA index area. The decline in shrimp postlarvae and other macrozooplankton may be due to a combination of factors, such as climate and ocean shifts (atmospheric easterly waves disturbances, winds, precipitation, salinity) and possibly increasing marine pollution (related to extreme rainfall events, that convey macro- and microplastics, and pollutants from the continent). Cnidarian medusae and fish eggs were among the few “winners” of this ecosystem regime shift. Changes in climate, ocean, macrozooplankton, and shrimp postlarvae abundance evidence a relevant climate, ocean and ecosystem regime shift in this region with a tipping point during 2015/16 “Godzilla” El Niño. Possible future consequences in the context of persistent warming in the TSA region and the currently ongoing record strength 2023/24 EN event are discussed.

Distribution and environmental preferences of Saprolegnia and Leptolegnia (Oomycota) species in fish hatcheries in Hungary

We studied for the first time the abundance and species distribution of water molds (Oomycota, Saprolegniales) in fish hatcheries in Hungary, with a focus on Saprolegnia and Leptolegnia spp. A total of 182 water mold isolates were obtained from different developmental stages of fish (eggs, fry, and adults) and water samples collected during the spawning season of cyprinids and salmonids. Of the ten water mold species identified based on their ITS–rDNA sequences, S. parasitica, S. ferax, and S. australis were the most abundant. Statistical analyses of species proportions in relation to the sample source and host preference revealed that S. parasitica was significantly more abundant in the host than in water samples, whereas the opposite trend was observed for S. ferax and S. australis. In addition, our results confirmed previous findings that S. australis and S. ferax prefer warm water (+21–24 °C) and that S. parasitica has a wide temperature tolerance (+3–24 °C). The DNA sequence identity analysis showed that the species demarcation criterion for Saprolegnia spp. was approximately 98 % identity, based on ITS–rDNA sequences. Furthermore, our phylogenetic analyses suggest that taxonomic revision of Leptolegnia spp. is necessary.

The influence of lingonberry and sea buckthorn powder-aqua solutions on the nutritional value of sander roe

Fish roe is a rich source of polyunsaturated fatty acids, essential amino acids and several vitamins. The paper presents the possibility of using fish caviar treatment with berry solutions instead of traditional preservatives. The purpose of this study is to determine the effect of berry solutions from lingonberry powder (Vaccinium vitis-idea) and buckthorn (Hippophae rhamnoides) on the nutritional, biological value and safety of walleye caviar through the use of wild plants as preservatives. Samples of the caviar of the common walleye Sander (Sander lucioperca) were selected as research objects. The results showed that experimental caviar samples treated with berry solutions showed differences (p&gt;0.05) compared to the control sample with saline solution, this proves an increase in the nutritional value and safety of fish caviar. It is also shown that the use of berry solutions from cranberries (LS) and sea buckthorn (SBS) combined with salt is 35% more effective compared to the control sample containing only salt (S). This means that using berry solutions significantly improves the results compared to the traditional canning method. This effect may be due to the additional beneficial properties of berry extracts, such as antioxidant and antimicrobial activity, which contribute to better preservation of caviar quality and safety. It was found that using a berry solution from cranberry powder revealed the best result. Also, it was shown that the greatest value and composition was possessed by the LS sample, which more satisfied the daily requirement of vitamins A, E and B group in comparison with other samples (p&gt;0.05), the content of vitamin A (0.016±0.014); vitamin B (0.24±0.16); vitamin E (2.89±0.04); vitamin PP (2.1±0.05). This is because treating pikeperch caviar with berry solutions increases the amount of water-soluble and fat-soluble vitamins.

The influence and mechanism of fish collagen peptide and egg yolk lecithin on proliferation and lipid composition in feline adipocytes.

The influences of fish collagen peptide (FCP) and egg yolk lecithin (EYL) on the proliferation, fat accumulation and triglyceride content in feline adipocytes were investigated in this work, aiming at unveiling the mechanism of fat accumulation for cheek of feline animals. The lipogenic changes of adipocytes in the presence of FCP and EYL were determined by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The results demonstrated that FCP of 10mg/mL had the strongest cell activity, with a relative increment rate of 156 ± 0.23%, and the triglyceride content reached 215.9 ± 3.86 mmol/L. By comparison, it was observed that an EYL concentration of 5mg/mL elicited the highest cell activity, exhibiting a relative increment rate of 152 ± 0.60%, and the level of triglyceride content was noted to reached 256.56 ± 25.68 mmol/L. After the feline adipocytes were treated with different concentrations of two active substances, fat formation and lipid droplets were found by oil red O staining. Liposome analyses confirmed that the formation of lipid compounds was regulated by FCP and EYL through pathways involved in lipid metabolism, notably including inositol phosphate insulin resistance, and phosphatidylinositol signaling pathways. This regulation was found to enhance cell vitality and facilitate fat accumulation. These findings provide a new strategy for the development of nutritional and healthy products or foods that promote feline cheek.