Salivary Alkaline Phosphatase Levels Research Articles

Reliability of maxillary canine calcification stages and salivary alkaline phosphatase for pubertal growth prediction (cross-sectional study)

Orthodontic growth estimation for growing individuals is essential for diagnosis and treatment planning, essentially in managing cases with skeletal growth defects. Research has revealed a substantial correlation between tooth calcification stages and skeletal maturity. Also, many biomarkers have been investigated as maturity indicators, and studies show promising results. Therefore, the present research assessed if salivary alkaline phosphatase (ALP) level and maxillary canine calcification stages with developmental stages of modified middle phalanx of the third finger (MP3) are reliable skeletal maturity indicators. No previous study conducted the reliability of both canine calcification stages and ALP level as skeletal maturity indicators. In this prospective observational study with a cross-sectional design, 80 subjects aged 8- 16 years were selected. Unstimulated saliva to assess ALP, periapical radiographs for the maxillary canine to assess the dental calcification by relying on the Dermirjian's stages, and periapical radiographs for the middle phalanx of the third finger region were taken from 80 individuals (from eight to sixteen years old) to assess the skeletal maturation state. The data were analyzed using the statistical package of social science (SPSS version 26), using the Spearmen correlation test and the Kruskal-Wallis test. The maxillary canine's calcification stages and MP3 stages showed a significant correlation coefficient; however, the correlation coefficient of MP3 stages and maxillary canine calcification stages with salivary ALP was non-significant. The maxillary canine calcification stages can practically be utilized as an indicator for assessing skeletal maturity, but the salivary ALP level showed no correlation with skeletal maturity indicators. Keywords: Skeletal maturity indicator; Canine calcification stages; Alkaline phosphatase; Middle phalanx of third finger.

Impact of dental anxiety on dental caries and salivary alkaline phosphatase in children across different nutritional statuses.

Dental anxiety is a familiar problem among children, which may affect their oral health. This cross-sectional study aimed to evaluate dental anxiety during the first dental examination in relation to dental caries and salivary alkaline phosphatase, taking into account the nutritional status of children. Eighty-two children (45 boys and 37 girls), aged six to eight years old (average 6.96), were recruited from public clinics in Baghdad city. Participants were divided into the anxious and the non-anxious groups according to physiological measures (oxygen saturation and heart rate) before the first dental treatment. The weight and height of each child were measured to determine the nutritional status (normal weight, overweight, and obese). Dental caries (prevalence and severity) in children with different nutritional statuses were recorded using dmf, DMF, and the guidelines outlined by Manjie et al. Saliva was collected to analyze salivary alkaline phosphatase. No statistically significant differences were observed between anxious and non-anxious children in means of dmf and DMF indices (teeth and surfaces) in primary and permanent teeth (p>0.05) in the three nutritional status groups. However, non-anxious children exhibited a higher mean of d1 (initial enamel caries) compared to anxious children, with statistical significance (p<0.05) observed only in normal-weight children. Salivary alkaline phosphatase was not significantly different between the two groups. In conclusion, dental anxiety did not appear to significantly impact caries development in primary teeth or salivary alkaline phosphatase levels in children with varying nutritional statuses. Anxiety reduced the initial enamel caries in normal-weight children.

Evaluation of Salivary Alkaline Phosphatase Levels in Passive Smokers of Different Age Groups.

Background The smoke inhaled by a nonsmoker from the smoldering end of a cigarette is referred to as passive smoke. The nicotine present in smoke is known to cause tissue damage and alter the enzymatic composition of the body. Alkaline phosphatase (ALP) is a group of intracellular hydrolytic enzymes known to partake in cellular metabolism. ALP levels are affected by smoking as well as passive smoking (PS) with a change in the pH of the oral cavity. The association of salivary alkaline phosphatase (S-ALP) levels in different age groups, gender, and times of exposure is not thoroughly explored yet, which was the primary aim of this study. Material and methods A total of 64 samples were collected from passive smokers and non-smokers. Unstimulated saliva (2-2.5 mL) was collected from each subject after obtaining their consent, followed by centrifuging and mixing with ALP reagent in a semi-autoanalyzer to obtain the S-ALP levels. Results Higher S-ALP levels were seen in passive smokers (34.70 IU/L) compared to healthy individuals (12 IU/L), which came to be statistically significant (p<0.01). S-ALP levels, when compared to different age groups and gender, were statistically insignificant (p>0.05). However, higher levels were seen in association with time of exposure in passive smokers where the data was statistically significant (p<0.01), suggesting tissue damage possibly due to oxidative stresses and tissue inflammation on continuous exposure for a minimum of 30-60 minutes daily as per our study. Conclusion Significantly high levels of S-ALP were found in passive smokers in comparison to non-smokers. This suggests that passive smoking has negative effects on the body tissues. Age, gender, and time of exposure of a non-smoker to tobacco smoke can lead to alterations in S-ALP levels. High levels of S-ALP were seen in individuals with prolonged exposure to tobacco smoke on a daily basis. Salivaomics can thus be used as a non-invasive, economical, and accurate alternative in tissue damage diagnosis.

Comparative evaluation of periodontal health status of pre and postmenopausal females.

Menopause in females is a corporeal stage that gives rise to a number of adaptive changes both at systemic and oral levels, prototypically occurring in the late fourth or fifth decade of life. Though physiological aging affects the oral tissue, the hormonal changes due to menopause also act as a major contributing factor in deteriorating the health of oral tissues. Thus, the main aim of our study was to compare the overall periodontal status and alkaline phosphatase levels in the saliva of females in their pre and postmenopausal ages. This study was conducted on 200 female subjects coming to the Department of Periodontology at Rajendra Institute of Medical Science for oral prophylaxis. The subjects were arbitrarily selected in the age group ranging from 15 to 70 years and were further divided based on the inclusion criteria. Group A included 100 subjects with age ranging from 15 to 45 years, and group B, 100 subjects with age 54 to 70 years. Signs of periodontitis including clinical attachment loss, furcation involvement, and probing depth and salivary alkaline levels were obtained, evaluated, compared, and analyzed. In group A 65% of patients had grade 0 and 28% had grade 1 of clinical attachment level. Similarly in group B, 44% of the total had grade 0 of clinical attachment loss, 38% had grade 1, and 18% were of grade 2. On evaluating grades of furcation involvement, around 45% of the total patients in group A were grade 1 (incipient, pocket formation), while in group B, 51% of the total patients were grade 1. At least 46% in group A and only 20% in group B had no signs of furcation involvement. Salivary alkaline phosphatase levels in pre and postmenopausal patients showed a significant difference between the two groups was obtained. The study concluded a major difference in the periodontal health of pre and postmenopausal women with many influencing factors apart from menopause itself.

Evaluation of salivary alkaline phosphatase levels in tobacco users to determine its role as biomarker in oral potentially malignant disorders

Background: Although elevated salivary alkaline phosphatase (S-ALP) levels have been seen in oral squamous cell carcinoma, the status of S-ALP in cigarette users and those with oral potentially malignant disorders (OPMDs) is less well understood. The study's goals and objectives were to assess and compare S-ALP levels in tobacco users, nonusers, and people with OPMD. Materials and Methods: The study included 150 people who were divided into four groups: those who did not use tobacco, tobacco smokers, those who are in habit of tobacco chewing, and those who are in the habit of tobacco chewing/smoking and had a lesion. unstimulated saliva (5 mL) was collected and centrifuged for 15 minutes at 3000 rpm, and the supernatant was separated. In an automatic analyzer, S-ALP was calculated in the supernatant using the kinetic photometric method. Results: The information gathered was subjected to statistical analysis. S-ALP levels were 17.90 IU/L in healthy people who didn't smoke, 4.58 IU/L in smokers who didn't have a lesion, and 7.52 IU/L in tobacco chewers who didn't have a lesion, and 64.92 IU/L in people with OPMD. Using Kruskal–Wallis' ANOVA, the mean difference between the groups was statistically significant (P 0.001).

Evaluation of salivary alkaline phosphatase levels in tobacco users to determine its role as a biomarker in oral potentially malignant disorders

Background: Saliva, a widely obtainable oral fluid, has gained acceptability as a screening medium in numerous health disorders in recent years. It has the capacity to discern initial epithelial alterations in tobacco abusers and people with OPMD since it is in direct contact with the lesion. Although few reports have been conducted on the use of concentration of alkaline phosphatase (ALP) enzyme in serum as well as saliva of OSCC patients as a biomarker, more research is needed in OPMD.Aims and Objectives: The study's major goals were to measure S-ALP levels in smokers, nonsmokers, and people with OPMD. and to compare S ALP levels in smokers, nonsmokers, and people with OPMD.Materials and Methods: The 84 participants in the study ranged in age from 18 to 75 years old, and they were divided into four groups:Category One – People who don't smoke or chew tobacco and don't have any lesions on intraoral inspection (n = 24).

Evaluation of salivary alkaline phosphatase levels in tobacco users to determine its role as a biomarker in oral potentially malignant disorders

The aim of this study evaluate the salivary alkaline phosphatase levels in tobacco users to determine its role as a biomarker in oral potentially malignant disorders. This prospective observational study comprised 60 individuals aged between 18 and 70 years who were categorized into four groups. For Group II, Group III and Group IV, individuals with the habit of smoking/tobacco chewing for a minimum period of 6 months were included in this study. The mean values for S‑ALP were found to be about 19.00 IU/L (SD 12.37) for Group I (range from 7 to 50 IU/L), 8.50 IU/L (SD 3.35) for Group II (range from 2 to 14 IU/L), 5.60 IU/L (SD 1.01) for Group III (range from 1 to 8 IU/L) and 65.90 IU/L (SD 50.70) for Group IV (range from 10 to 146 IU/L). Comparison of S‑ALP between the groups showed a statistically significant difference (P &lt; 0.001) using Kruskal–Wallis’ ANOVA. To know if there is any difference in S‑ALP levels between tobacco users and nonusers (Groups I and II and Groups I and III) and among the different forms of tobacco users (Groups II and III), the mean S‑ALP levels were compared using Mann–Whitney U‑test.

Salivary IGF-1 and Alkaline Phosphatase-Based Modeling for Skeletal Maturity Prediction in Orthodontic Patients.

Results A strong positive correlation was found between CA and cervical stages (r = 0.836, P < 0.001). Based on the regression model analysis, the model which combined IGF-1, ALP, and CA provided the best prediction at P < 0.001 with McFadden's pseudo R2 value of 0.552 for cervical stage prediction and 0.646 for growth phase prediction. In particular, its predictive ability for the prepubertal, pubertal, and postpubertal growth phases was 95%, 80%, and 90%, respectively. Conclusions A new model with the combination of salivary IGF-1 and ALP with CA can be used as a less invasive method to determine the cervical stage and also growth phase with high predictive ability in healthy subjects.

Evaluation of Salivary Alkaline Phosphatase and Glutathione Peroxidase Levels in Diabetic and Nondiabetic Participants With and Without Smoking Habits: A Case-Control Study.

Background:The majority of the current evidence suggests that tobacco smoking increases the risk of diabetes. Salivary alkaline phosphatase (ALP) and glutathione peroxidase (GSHPx) considered a biomarker to detect various oral diseases. Several studies suggest that smoking habits tend to alter ALP and GSHPx levels. However, at present, there is no much information about these enzymes in smokers with diabetes. Hence, the study aimed to evaluate the status of salivary ALP and GSHPx levels in diabetic and nondiabetic participants with and without smoking habits.Materials and Methods:This case–control study was approved by the Institutional Ethical Committee. A total of 60 male participants between the age group 35–50 years were recruited. Informed consent was obtained from participants. Participants were categorized into four groups: Group I – Smokers with diabetes (n = 15), Group II – Smokers without diabetes (n = 15), Group III – Nonsmoker with diabetes (n = 15), and Group IV – Nonsmoker without diabetes (n = 15). Salivary ALP levels and GSHPx activity were measured by colorimetric assay. Data were compared between groups using the one-way analysis of variance, followed by a Bonferroni post-hoc test.Results:Nonsmoker diabetic participants demonstrated significantly higher ALP levels as compared to other groups (P < 0.05). We observed significantly lower levels of ALP in smokers with diabetes (P < 0.05). We observed a significant decrease in GSHPx activity in smokers with diabetes compared to all other groups (P < 0.05).Conclusions:Salivary ALP can be used as a clinical biomarker to be correlated for evaluating diabetes. GSHPx activity can be used to understand the response of supplementation therapy in smokers with diabetes.

The relationship between chronological age, dental age, and salivary alkaline phosphatase in Indonesian children aged 8–14 years: A cross-sectional study

Inability to predict dental age accurately has long been the Achilles heel of pediatric dentistry. Whilst dental age has an important aspect in clinical practice, saliva can be one of the most practically important diagnostic tools to estimate biological age. This study aimed to analyze a correlation between chronological age, dental age, and salivary alkaline phosphatase in Indonesian children aged 8–14 years. This study was an observational study with cross-sectional design. Twenty healthy children (10 boys and 10 girls) were selected by consecutive sampling. Chronological age was assessed by recording date of birth. Dental age was assessed by orthopantamogram following Demirjian's method. Salivary samples were collected by passive drool method and estimation of alkaline phosphatase was done by autoanalyzer. Normality test was assessed using Kolmogorov-Smirnov and Shapiro-Wilk test. Statistical analyses were assessed using Spearman's rank-order correlation coefficients. Results are presented as mean ± standard deviation (SD). Mean chronological age was 10.504 ± 1.895 years, mean Demirjian's score was 91.74 ± 5.972, and mean salivary alkaline phosphatase was 291.563 ± 115.135 pg/ml. There is a very strong positive correlation between chronological age and dental age (r = +0.804; p < 0.001). On the contrary, levels of salivary alkaline phosphatase was negatively correlated to dental age (r = −0.780; p < 0.001) and chronological age (r = −0.508; p = 0.022). This study showed strong correlation between dental age, chronological age, and salivary alkaline phosphatase; hence, estimation of dental age and salivary alkaline phosphatase in Indonesian children during early and late childhood has significant meaning to chronological age.

A Comparative Evaluation of Salivary Alkaline Phosphatase Level in Pre-Menopausal And Post-Menopausal Women With And Without Periodontitis. A Biochemical Study.

Introduction- Alkaline phosphatase is an intracellular destruction enzyme in the periodontium, and it takes part in the normal turnover of the periodontal ligament, alveolar bone, and root cementum formation and maintenance.&#x0D; Aim- The aim of this case control study was to evaluate the enzyme Alkaline Phosphatase (ALP) level in saliva of post menopausal women with and without chronic periodontitis.&#x0D; Materials and Methods- This study includes 48 samples of pre and post menopausal females. All the subjects were selected from the Department of Periodontology, Peoples College of Dental Science and Research Centre, Bhopal. The study participants were divided into 4 groups, Group A: 12 pre- menopausal women without periodontitis with systemically healthy, Group B : 12 post-menopausal women without periodontitis with systemically healthy, Group C: 12 pre- menopausal women with periodontitis and systemically healthy, Group D: 12 post- menopausal women with periodontitis and systemically healthy. Clinical parameters assessed were Plaque Index (PI), Gingival Index (GI), Clinical Attachment Level (CAL) and Probing Pocket Depth (PPD). Unstimulated salivary samples were obtained in which the ALP concentration was measured using p-Nitrophenylphosphate, and 2-amino-2-methyl-1-propanol reagents in Beckman and Coulter, AU 480 auto analyser. Mann-Whitney U test was used to find statistical difference with respect to all clinical parameters such as PI, GI, CAL, PPD and salivary ALP levels.&#x0D; Result – The result of the present study showed considerably higher salivary ALP levels in post menopausal women with chronic periodontitis compared to other groups.&#x0D; Conclusion -The present study suggest that the presence of ALP levels is not simply a reflection of the local inflammatory state in females but their estrogen levels may possibly influence bone destruction in periodontitis.

Effect of nonsurgical periodontal therapy on the salivary ghrelin and serum alkaline phosphatase levels in health and chronic periodontal diseases: A clinico-biochemical study

Effect of nonsurgical periodontal therapy on the salivary ghrelin and serum alkaline phosphatase levels in health and chronic periodontal diseases: A clinico-biochemical study

Salivary Alkaline Phosphatase Level in People with Healthy Periodontium and Generalised Chronic Periodontitis

Introduction: Periodontal disease is a multifactorial chronic inflammatory disease affecting the soft and hard supporting structures of teeth leading to alveolar bone loss and exfoliation of involved teeth when left untreated. Traditional periodontal diagnostic methods are not always able to assess present disease states. Saliva as possible biological sample helps measure current disease state, monitor treatment, and indicate prognosis. Oral fluids contain mediators of disease that include microbial host response and bone-specific resorptive markers.&#x0D; Objective: To investigate and compare salivary alkaline phosphatase level in people with healthy periodontium and generalised chronic periodontitis.&#x0D; Methods: This cross-sectional analytical study was conducted in the department of dental surgery, Bir hospital from January to December 2017. Ethical clearance was obtained from institutional review board. Unstimulated saliva sample for the evaluation of alkaline phosphatase was collected from 35 generalised chronic periodontitis (GCP) patients and 35 volunteers with healthy periodontium after informed consent was obtained and periodontal parameters were recorded. Data collected was analysed using SPSS software v.16.&#x0D; Results: Three and half fold increased level of salivary alkaline phosphatase level in GCP patients than healthy volunteers was found which is highly significant. Correlation of salivary alkaline phosphatase level with clinical features of the chronic periodontal disease was observed.&#x0D; Conclusion: An expected benefit of an oral fluid-based periodontal diagnostics would be identification of highly susceptible individuals prior to aggressive disease. Timely detection and diagnosis of disease offer earlier, less invasive, and more cost-effective treatment.

Serum and salivary alkaline phosphatase levels in oral health and disease: A brief review

Alkaline phosphatase is an intracellular hydrolase enzyme participating in the metabolic processes of cells. The enzyme alkaline phosphatase (AP) occurs in many organisms ranging from bacteria to man. Alkaline phosphatase levels were increased in various disease processes like periodontitis, gingivitis, and bone disorders. The alkaline phosphatase enzyme released in secretions like the serum, saliva, and gingival crevicular fluid. The serum and salivary levels were used as a diagnostic aid in oral diseases. The below was a brief review of the alkaline phosphatase levels both in serum and saliva.

Detection of Salivary Alkaline Phosphatase Levels in Smokers, Diabetic Patients, Potentially Malignant Diseases and Oral Malignant Tumours.

ABSTRACTAim:Alkaline phosphatase (ALP) is present in human and plays a key role in intracellular destructive processes and cellular damage. It has bicarbonate and phosphate ions, which help in buffering against acids. ALP activity is affected by smoking, which changes pH in oral environment and has harmful effects. Thus, the evaluation of ALP activity of smokers and healthy nonsmoker along with patients who were diabetic, potentially malignant, and malignant was carried out in this study.Materials and Methods:The study took place between 2016 and 2017. A total of 150 smokers, non-smokers, and patients who were diabetic, potentially malignant, and malignant were included. Collection of unstimulated whole saliva was done from each participant, and salivary ALP levels were measured by spectrophotometric assay.Results:Mean salivary ALP levels were significantly higher in smokers compared to those in nonsmokers. Mean ALP levels were also increased in patients who were diabetic, potentially malignant, and malignant compared to those in controls.Conclusion:ALP levels were significantly higher among smokers when compared to a healthy control group. Oral tissue function and side effects among smokers can be evaluated by these salivary enzyme alterations, which can serve as biomarkers for the diagnosis of any disease process. These salivary alterations could potentially be used as biochemical markers for the evaluation and early diagnosis. The higher levels were also found in patients who were diabetic, potentially malignant, and malignant, and ALP levels may also be used as biomarkers for the evaluation of the disease process.

Salivary Alkaline Phosphatase Level Changes in Patients with Multiple Implants Surgery (Comparative Study)

Salivary Alkaline Phosphatase Level Changes in Patients with Multiple Implants Surgery (Comparative Study)

Evaluation of salivary alkaline phosphatase levels in tobacco users to determine its role as a biomarker in oral potentially malignant disorders.

Background:Elevated salivary alkaline phosphatase (S-ALP) levels have been observed in oral squamous cell carcinoma, but its status in tobacco users and in individuals with oral potentially malignant disorders (OPMDs) is less explored.Aims and Objectives:The aims and objectives were to estimate and compare the levels of S-ALP among tobacco users, nonusers and in individuals with OPMD.Materials and Methods:The study population comprised 42 individuals, categorized into four groups with/without tobacco usage habit and with/without lesion. 5 ml of unstimulated saliva sample was collected, centrifuged at 3000 rpm for 15 min and supernatant separated. S-ALP was estimated in the supernatant by using kinetic photometric method in an automatic analyzer.Results:Data obtained were subjected to statistical analysis. The mean S-ALP was 18.00 IU/L for normal individuals without tobacco usage, 4.60 IU/L for smokers without lesion, 7.50 IU/L for tobacco chewers without any lesion and 64.90 IU/L for individuals with OPMD. The mean difference between the groups was statistically significant (P < 0.001) using Kruskal–Wallis’ ANOVA. No statistically significant difference (P > 0.05) was obtained in the S-ALP levels between tobacco users and nonusers and between smokers and tobacco chewers, using Mann–Whitney U-test. S-ALP levels in individuals with OPMD were statistically significantly higher (P < 0.001) than those without lesions, with or without tobacco usage habit, using Mann–Whitney U-test.Conclusion:We conclude that S-ALP could be used as a reliable noninvasive biomarker in monitoring OPMD.

Comparative Evaluation of BMI, Dental Age, Salivary Alkaline Phosphatase Levels, and Oral Health Status in Children with β Thalassemia Major.

ABSTRACTPurposeThalassemia poses clear systemic and oral health problems. Clinicians must be aware of various dental treatment needs in these patients. This study was undertaken to assess body mass index (BMI), dental age, salivary alkaline phosphatase levels, malocclusion, and treatment needs in children with β thalassemia major.Materials and methodsA total of 100 children within the age group of 2–15 years (50 thalassemic and 50 healthy controls) were included. Dental age, oral health status, malocclusion, and intervention urgency index was recorded. Unstimulated whole saliva samples were collected to assess the salivary alkaline phosphatase levels. The recorded data were statistically analyzed.ResultsA significantly greater number of thalassemia children were found to be in the underweight category. A significantly high OHI-S scores and low gingival index scores were observed in thalassemia children. Salivary alkaline phosphatase levels were significantly low in thalassemia children compared to the control group. Intervention urgency index scores were significantly high in thalassemia group of children.ConclusionThalassemia children had low BMI, gingival index scores, salivary alkaline phosphatase levels and higher prevalence of malocclusion, and high OHI-S index scores compared to the healthy control group. Intervention urgency index scores revealed greater dental treatment needs in children with thalassemia.How to cite this articleRani ST, Reddy ER, et al. Comparative Evaluation of BMI, Dental Age, Salivary Alkaline Phosphatase Levels, and Oral Health Status in Children with β Thalassemia Major. Int J Clin Pediatr Dent 2019;12(4):303–306.

Evaluating Salivary Alkaline Phosphatase Levels as a Biochemical Marker of Periodontal Disease in Periodontal Patients in a Tertiary Hospital in Nigeria

Background and aims. Traditional methods of diagnosing periodontal diseases have their limitations, which include inability to provide information about the recent activity of the disease and difficulty in diagnosing the disease at an early stage when clinical changes have not started. This has necessitated the use of biomarkers designed to overcome these shortcomings. Materials and methods. A cross-sectional investigation was conducted among three groups of 27 participants each to assess the level of salivary alkaline phosphatase (SALP). The groups consisted of a control group (healthy) and two test groups (gingivitis and periodontitis). Saliva samples were collected and spectrophotometric analyses were carried out. Results. The highest mean SALP level (87.78 U/L) was found in group III, followed by group II (73.56 U/L), with group I exhibiting the least (38.89 U/L). The differences in the mean SALP between groups I and II, II and III, and I and III were statistically significant. Correlation between periodontal pockets depth and mean SALP was significant in group III. Conclusion. Variations in mean SALP levels among subjects with different periodontal conditions showed that mean SALP can differentiate various degrees of periodontal conditions and therefore has the potential to be used as a biochemical marker for periodontal disease.

Estimation of salivary and serum alkaline phosphatase level as a diagnostic marker in type-2 diabetes mellitus with periodontal health and disease: A clinico-biochemical study.

Introduction:Chronic periodontitis is a multifactorial disease resulting in the inflammation and destruction of the supporting structures around the teeth, leading to tooth mobility and subsequent loss of tooth. Metabolic disorders, such as diabetes mellitus, play a crucial role in the progression of periodontal inflammatory conditions. Alkaline phosphatase (ALP) enzyme plays a key role in gingival inflammation and bone resorption. Hence, the aim of the present study is to compare the serum and salivary alkaline phosphatase levels in chronic periodontitis patients with or without type-2 diabetes mellitus.Materials and Methods:A total of 45 individuals were included in the study and divided into three groups: Group I (healthy individual), Group II (Chronic periodontitis without diabetes mellitus type-2) and Group III (Chronic periodontitis with type-2 diabetes mellitus) on the basis of clinical, radiographic and blood sugar examination. The serum and unstimulated saliva were collected from all patients in aseptic condition and samples were analyzed for alkaline phosphatase level using AVANTOR™ Benesphera ALP Kit by fully automated analyzer.Results:The result showed that the concentration of serum and salivary alkaline phosphatase increases significantly in patients with chronic periodontitis with type-2 diabetes mellitus than chronic periodontitis without diabetes mellitus and healthy patients.Conclusion:We can conclude that alkaline phosphatase can be used as a key inflammatory diagnostic biomarker in periodontal diseases.