Austenitic stainless steel welds (ASSWs) suffer from severe pre-strain during assembly process, which threatens the long-term operation safety of pipelines in nuclear power plant. In this work, the 316L weld metals (WMs) with 0 % and 8 % pre-strain were thermally aged at 400 °C for up to 39000 h to investigate the pre-strain effect on thermal aging of ASSWs. The results showed that the pre-strain caused work hardening and further promoted the hardening of thermally aged ferrite. After thermal aging for 39000 h, the nano-hardness increment of ferrite with 8 % pre-strain was about 1.6 GPa more than that without pre-strain. By microstructure characterization, it is found that the high dislocation density induced by pre-strain promoted spinodal decomposition and G-phase precipitation. The spinodal decomposition morphology and corresponding element concentration fluctuations were more obvious in the WM with 8 % pre-strain. Moreover, the size and density of G-phase along dislocations were larger in the ferrite with 8 % pre-strain than those without pre-strain.