In the post-socialistic space, old, industrialised regions are not only dealing with problems with the regeneration of industrial brownfields but also with the occurrence of post-agricultural brownfields. The main objective of this paper is to identify and interpret how a successful network of cooperating actors was formed that participated in the process of transforming an agricultural brownfield into a successful rural business zone. From a methodological perspective, the article is based on the findings derived from a set of written memories with ten key actors who were involved in the redevelopment process. The studied regeneration project, which was implemented thanks to the successful cooperation of network of actors both from different hierarchical levels (local, regional and central level) and from the different sectors (public, private and non-profit sector), regenerated an area of 10.4 hectares thanks to both public and private investments of approximately CZK 240 million. Regeneration contributed to the creation of approximately 450 new jobs, and thanks to this project, the population in Třanovice also increased significantly in the transition period (1991–2021), which the studied community positively distinguishes from the shrinking urban centres and rural communities in the post-industrial neighbourhoods.
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