ABSTRACTDissolved organic carbon (DOC), an organic carbon fraction with high activeness and mobility, migrated by runoff is a key part in carbon cycle. A rational straw mulching rate can be regulated to obtain maximum benefits while controlling runoff and sediment yield on sloping lands. However, little remains known about the optimal straw mulching rates required for effectively reducing the loss of DOC in runoff. Therefore, to overcome the existing limitations, this study investigated the effects of modified maize straw mulching rates on the loss of DOC during runoff, utilizing indoor rainfall simulation. Five mulching rates, including 0, 0.2, 0.4, 0.6, and 0.8 kg m−2 [control (CK) and treatments (T1, T2, T3, and T4), respectively], were tested in combination with three slope gradients (10°, 15°, and 20°) to evaluate how straw mulching rate influences runoff, sediment yield, and runoff‐related DOC loss under a heavy rainfall intensity of 90 mm h−1. Our results showed that various straw mulching rates did not significantly differ runoff rates; however, straw mulching significantly reduced sediment concentration and yield. Moreover, the reduction in sediment yield increased with an increase in mulching rate. Compared to the CK, T1 resulted in a 63% increase in DOC loss at a slope of 20°. Additionally, T2 caused an 8% and 7.2% increase in DOC loss at both 10° and 15° slopes. Conversely, T3 and T4 reduced DOC loss by 54.1%–80.8% and 51.1%–65.2%, respectively, across all slope gradients. These results suggested that mulching rates of 0.2–0.4 kg m−2 may potentially increase DOC loss in runoff on the sloping lands. Our results hold significant importance in optimizing the use of straw mulching for sustainable management practices in agricultural lands.