Hybrid bi-material concepts in engineering structures where fibre-polymer composites are used together with steel structural members have potential to reduce material usage, extend fatigue life and improve structural reliability. The bond performance of the bi-material composite-to-steel interface is of crucial importance and highly relies on the surface preparation quality of the steel element. This paper investigates the influence of steel surface roughness on the mode II fracture toughness and fatigue crack growth behaviour of the bonded composite-to-steel joints. Glass fibre composite and mild structural steel material is considered directly bonded in a wet lay-up process. 4-point end notch flexure (4ENF) tests are conducted on specimens with the steel plates prepared with low, medium and high roughness, respectively. In addition, morphology of the fracture surfaces are characterised by a 3D profilemeter and the friction coefficient is measured by a tribometer for each roughness level. Results show that in quasi-static fracture, fibre bridging is dependent on the surface roughness. A roughness level with Sq ≥ 22 µm of the steel surface can promote significant fibre bridging thus improved fracture toughness due to enlarged effective bonding area. Under cyclic loading, no fiber bridging is observed across all roughness levels tested. However, the Paris curve parameter C is significantly affected by the roughness level, which decreases by approximately 100 times as the steel surface roughness increases from the low level of Sq = 5 µm to high level of Sq = 22 µm. The m parameter of the Paris curve remains fairly constant across all the roughness levels tested.