In the Pampas region of Argentina agriculture is dominated by intensive no-till (NT) soybean cropping which produce negative consequences on soil quality. A group of farmers started to use the Good Agricultural Practices (GAP) which include a higher crop rotation, use of winter cover crops and nutrient restoration. In this NT system earthworms have a significant role in soil functioning, particularly in organic matter cycling and soil structure formation. The aim of this paper was to examine the contribution of earthworm activity to the process of C incorporation and soil structure maintenance in soils with different NT variants of: NT with GAP for 30 years (NT + r30); NT with some of the GAP for 12 years (NT-r12) and NT with soybean monoculture (NTm). Also a natural grassland (NA) was sampled as a reference. Earthworm aggregates were obtained by gently separating them from surrounding soil. Fine (HOC) and coarse (POC) organic matter fractions, water-stable aggregates (WSA) and mean weight diameter (MWD) were calculated for earthworm aggregates and bulk soil. In all sites only one species (Aporrectodea caliginosa) was found, with higher density in the NA, followed by NT + r30, that had about 9 times more earthworms than NTm. The number of earthworm aggregates was higher in the NT + r30 followed by the NA, both showing differences with the other NT systems. The earthworm aggregates in NA and NT + r30 had significantly more POC than the surrounding soil (230% increase in NA and 100% NT + r30). Earthworm casts had higher values of MWD and WSA than physical soil aggregates in both NA and NT + r30 management treatments. Our results show the existence of a positive feedback loop we called earthworm-driven virtuous cycle. The increase in earthworm abundance promotes higher production of earthworm aggregates which are richer in organic matter and more water stable than the surrounding soil. This has favourable results in terms of soil quality but also increase crop yields (57% in maize and 18% in soybean), by means of biologically mediated soil processes, which is a highly desirable way to sustainability of agricultural production. Farmers, politicians and the whole society should pay more attention to soil as a key component supporting agricultural production by means of internal biological soil functioning.