Rosella Flower Research Articles

Karakteristrik Enkapsulat Ekstrak Pewarna Fungsional Bunga Rosella (Hibiscus sabdariffa L.) pada Perlakuan Perbandingan Kasein-Maltodekstrin

Rosella contains high levels of anthocyanin and can be used as a source of functional dyes. The making of functional dyes is carried out through the process of extraction and encapsulation. The aims of this study were to determine the effect of casein-maltodextrin encapsulation ratio on the encapsulation characteristics of rosella flower functional dye extract and determine the best encapsulation ratio of casein-maltodextrin in producing rosella functional dye extract encapsulates. Experiments in this study used a single factor randomized block design. These factors are the casein and maltodextrin ratio which consists of (maltodextrin), (1: 0.5), (1: 1), (1: 1.25), (1: 1.5), (1: 1.75), (1: 2.0), (1: 2.5) and (casein). The results showed that casein and maltodextrin comparison factors had a significant effect of yield, (P <0.01) on yield, total anthocyanin, surface anthocyanin level, encapsulation efficiency, solubility, brightness (L*), redness (a*), yellowish level (b*) but did not have a significant effect (P> 0.05) on water content. The comparison treatment between casein and maltodextrin 1: 0.5 is a treatment to produce encapsulate products of roselle flower functional dye extract with a yield of 42.86 percent, water content of 5.04 percent, surface anthocyanin total of 9.43 mg/g, surface anthocyanin level 1.66 mg/g, encapsulation efficiency 82.37 percent, solubility 45.84 percent, brightness level (L*) 60.86, redness level (a*) 25.64, yellowish level (b*) 29.61.
 Keywords: rosella (Hibiscus sabdariffa L.), extraction, casein, maltodextrin, encapsulation.

Pengaruh Pemberian Seduhan Rosella Terhadap Penurunan Kadar Asam Urat Wanita Menopause

Gout disease is a commonly affects men in aged 30-40 years old, while this disease occurred in women in aged 55-70. The lack of estrogen level is contributing to increase the incidence of gout disease in menopause woman. Menopausal women have two times higher risk of gout disease due to deficit role of estrogen in the removal of uric acid trough urine system. The decrease of estrogen hormone level affect the function of excretion system (Integument and kidney) and also digestive system. While the function of these systems decreased, the residual compound like purin could not be removed trough urine, feces, and sweat. The production of uric acid in human body were linear to the breakdown process of purin. The more purines broken down, the more uric acid is produced. One of the plants that can reduce the uric acid level is Rosella (Hibiscus sabdariffa). The anthocyanins pigment in the calyx of Rosella flower could produce flavonoid compound. The question research of this study is the administration of steeping rosella could reduce the uric acid level in menopause women. Objective: To determine the effect of rosella steeping on decreasing uric acid levels in menopausal women. Method: Pre-experimental with the design of one group pre-post-test have been done, the analysis is using the Wilcoxon test signed rank test. The analysis of results obtained by t-test Sig. (2-tailed) 0.001 which value <0.05 is significantly different. The Rosella steeping administration could affect the decrease of uric acid levels in menopausal women.

IMPROVING POST-PARTUM HEALTH USING HERBAL SOURCES

The postpartum period is a time after the placenta is born until 40 days later, during this period there is a process of returning the physical condition of the mother after conceiving and giving birth. In the process of returning this physical condition requires more attention because there is a possibility of upcoming problems such as bleeding, infection, breastfeeding disorders, vascular disorders, and psychological. There have been several efforts made to support the improvement of postpartum maternal health, both using professional care and through traditional treatments such as the use of herbs. The purpose of this study was to identify the benefits of herbal medicines and their effectiveness in improving mothers’ health in the puerperium. This study used a literature review approach through search in the Google Scholar, EBSCO, and Proquest databases with keywords: herbal, post-partum, and health promotion. In this study, 12 journals were obtained regarding the use of herbs used by women in the puerperium period to improve their health. Herbs used to improve maternal health include: purple sweet potato leaves, katuk leaves, turmeric, lempuyang, tamarind, green bean extract, black meeting, aloe vera, kalendula flowers, binahong leaves, rosella flowers, bitter orange, and lavender. The use of this herb serves to help repair reproductive organs to recover as before pregnancy. Keywords: Herbal, health promotion, postpartum.

PERENDAMAN PLAT RESIN AKRILIK POLIMERISASI PANAS PADA EKSTRAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP PERUBAHAN WARNA

The color of acrylic resin plate is one of particular concern, because it's related with aesthetics of oral tissue. An acrylic resin can absorb colored fluids and cause a color change, which reduces the aesthetic value of the denture. This study aims to determine discoloration of acrylic resin plate heat cured after submersion of rosella flower extract. This study used 25 samples, divided into 5 samples for 20% rosella flower extract, 5 samples for 40% rosella flower extract, 5 samples for 60% rosella flower extract, 5 samples negative control (aquades) and 5 samples positive control (fifftydent). Soaking time is 10 minutes per day for a week. The type of research used was experimental pretest-posttest with control group design. Measurements using Adobe Photoshop and the CIELab color measurement system. Furthermore, statistically using the Shapiro wilk test, Leven's test, One Way Anova and Least Significant Diffence. The results of the study are shows the mean color change of CIELab (DE) in the five groups after treatment significantly different (p <0.05). Least Significant Difference - test (LSD) test shows the results of immersion test on extract 20% and 40% are not different from negative controls, extract 60% is different from negative controls, extract 60% is same like positive control. The conclusions in this study are concentration of immersion with rosella flower (Hibiscus sabdariffa L.) extract 20% and 40% can't change the color of the acrylic resin plate heat cured, however extract rosella flowers (Hibiscus sabdariffa L.) 60% can change the color of  acrylic resin plate heat cured.

PERBEDAAN pH SALIVA SEBELUM DAN SETELAH BERKUMUR DENGAN REBUSAN BUNGA ROSELLA PADA MAHASISWA JURUSAN KEPERAWATAN GIGI BUKITTINGGI

Polyphenols and flavonoids of roselle ( Hibiscus sabdariffa L ) acts as an antiviral, antioxidant and antibacterial that can affect bacteria and increase the pH of saliva in the oral cavity. The purpose of this study to determine differences in the pH of saliva before and after rinsing with stew rosella The design is a quasi-experimental study with pretest and posttest. The population in this study Dental students from the Department of Nursing. The sampling technique is purposive sampling. Data collected by direct observation and examination of the salivary pH. Analysis of the data used using paired t test.Results showed before rinsing with rosella flower stew, average salivary pH 6.6 (acidic) and after rinsing with rosella flower stew average salivary pH to 7.0 (neutral). Statistical test Paired t test showed p value = 0.00 <0.05. Conclusion of the study that there is a significant difference between the pH of saliva before and after rinsing with stew rosella. Suggested to the research subjects to use a mouthwash of natural materials such as stew rosella to maintain oral health.

UJI DAYA HAMBAT EKSTRAK KELOPAK BUNGA ROSELLA (HIBISCUS SABDARIFFA L.) SEBAGAI ANTIBAKTERI STAPHYLOCOCCUS AUREUS

Latar Belakang : Penyakit infeksi merupakan salah satu masalah kesehatan terbesar di dunia. Menurut WHO 2015 berdasarkan data YLL (Years Of Life Lost) di negara berkembang penyakit infeksi masih merupakan penyebab kematian utama. Staphylococcus adalah penyebab utama infeksi bernanah pada manusia yang terdapat di rongga hidung dan kulit sebagian besar populasi manusia. Salah satu Penyakit kulit yang disebabkan oleh bakteri Staphylococcus aureus adalah bisul. Staphylococcus aureus telah mengalami resistensi terhadap antibiotik. Maka dari itu alternatif yang dapat dilakukan adalah dengan cara menggunakan bahan alam atau tradisional yaitu dengan memanfaatkan kelopak bunga rosella (Hibiscus Sabdariffa L.). Tujuan penelitian ini adalah mengetahui daya hambat ekstrak bunga rosella (Hibiscus Sabdariffa L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Metode : Penelitian ini merupakan penelitian Deskriptif dengan empat perlakuan dengan variasi kosentrasi 70%, 75%, 80% dan 85% dengan menggunakan analisa data uji statistik Univariat.Hasil : Diameter zona hambat yang terbentukpada konsentrasi 70% sebesar 14.4 mm,konsentrasi 75% sebesar 15.7 mm, konsentrasi 80% sebesar 16.4 mm, konsentrasi 85% sebesar 19.6 mm.Kesimpulan :Ekstrak etanol kelopak bunga rosella (Hibiscus Sabdariffa L.) memiliki zat antimikroba yang dapat menghambat pertumbuhan bakteri staphylococcus aureus paling baik pada kosentrasi 85% dengan rerata zona hambat 19,6 mm dan di kategorikan kuat.

Efek Penambahan Sari Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kualitas Selai Lembaran Dami Nangka (Artocarpus heterophyllus)

Straw from jackfruit so far is still considered a waste that is still thrown away by most people and even often becomes a problem that can pollute the environment. Jackfruit straw contains many of the same substances as fruit flesh such as protein, crude fiber, sugar and so on. Jackfruit straw has pectin which can be used as raw material in making jam. In making jam, fruits that have high acidity are also needed. Rosella contains vitamin C and flavonoids in the form of antosanin which can be used as flavor enhancers and natural dyes. The aim of this study was to determine the addition of rosella flower concentration that produced the best jackfruit straw jam based on the level of panelist preference (texture, color, taste), anthocyanin content, vitamin C content. This study used a completely randomized design with 5 treatments consisting of concentrations 0%, 10%, 20%, 30% and 40% with 5 repetitions. Analysis carried out by ANOVA test, if there is an effect of giving rosella juice then it will be followed by Duncan's test. The results showed that the addition of 40% roselle flower extract affected the level of preference for texture, color and taste. The addition of 40% has the highest total anthocyanin content of 45.22 mg / 100g and the total content of vitamin C in jackfruit straw sheet jam is 35.11 ppm.
 
 Keyword : Jam sheet, jackfruit, rosella essence, levels of pleasure, anthocyanin contains, vitamin C contains.

ANALYSIS OF BONE IMPLANT TO CONTACT VALUE USING SCANNING ELECTRON MICROSCOPE IN POST IMMEDIATE IMPLANTS WITH ADDITION OF GEL-EXTRACTED ROSELLA FLOWER

Objective: The objective of the study was to analyze the difference of bone to implant contact (BIC) value post-immediate implants with or withoutinjecting rosella flower extracts gel in mongrel.Methods: A total of 18 implants were inserted in 9 mongrel’s dog. The implant was divided into two groups. The first one (test implant) was implantedimmediately after injection of gel-extracted rosella flower 10%, while another group (control implant) was implanted immediately after the toothextraction. The histomorphometry analysis was done after 14, 28, and 56 days to find out the BIC value.Result: The mean value of BIC rosella group was 63.44% and the mean value of the control group was 52.11%, with p=0.041.Conclusion: Rosella flower extract is able to shorten the inflammatory phase after implantation, accelerate and expand bone growth by spurringcollagen deposition as well as inhibiting osteoclast activity.

EFEKTIVITAS PENAMBAHAN EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa. L) TERHADAP AKTIVITAS ANTIOKSIDAN MINUMAN LIDAH BUAYA (Aloe vera)

Aloe vera and rosella flower has high antioxidants and has the potential as a functional drink. The purpose of this study was to analyze the antioxidant activity of aloe vera drink with the addition of various concentrations of rosella extract and stored for 21 days. The results showed that aloe vera drink with the addition of rosella flower calyx extract had a significant effect on antioxidant levels. The addition of rosella flower calyx extract at a concentration of 3% has the highest antioxidant, which is 58.12% after being stored for 21 days. Increasing the concentration of rosella flower calyx extract also significantly affected pH and total acid values. Aloe vera drink with the addition of 3% rosella extract after storage of 21 days had a total acid value of 6.6% and a pH value of 3.86. Different results are shown in the analysis of sensory tests, where the addition of rosella flower calyx extract to aloe vera drinks did not significantly affect the reception of panelists.

The utilization of red seed guava and rosella flower as sources of vitamin C

The research aimed at making instant drinks by using red seed guava androselle flowers as sources of vitamin C using a vacuum oven. The research was conducted in Laboratory of Processing and Management of Agricultural Products and Instrument Laboratory, Faculty of Agriculture, Universitas Katolik Santo Thomas Indonesian. This research was conducted with factorial random design with two treatments, namely: Factor I. The concentration of dextrin with K code consisting of 4 levels, namely: K1 is 40%; K2 is 50%; K3 is 60%; and K4 is 70%. Factor II. Drying with L code, consisting of 4 levels, namely: L1 is 15 hours; L2 is 20 hours; L3 is 25 hours and L4 is 30 hours. Parameters analyzed were rendement, vitamin C content, total acid, organoleptic value and water content. The best quality was obtained from rendement, vitamin C content, total acids, organoleptic value and water content with 60% dextrin concentration in 25 hours drying time.

PENGARUH WAKTU PERENDAMAN TiO2 DALAM LARUTAN EKSTRAK ANTOSIANIN BUNGA ROSELLA PADA KINERJA DYE SENSITIZED SOLAR CELL (DSSC)

The use of solar panels is still quite expensive in the making, for it needs to be developed solar panels are cheap based DSSC (Dye Sensitized Solar Cell). The use of DSSC is very good developed in Indonesia which is famous for its biological richness. This study used rosella flowers as photosensitizer obtained from the extraction process in the form of maceration for 24 hours. The components used to construct a DSSC outline can be divided into four parts, namely substrate, dye, semiconductor titanium dioxide (TiO2), and electrolyte. In this research the Ti O2 layer was immersed in a solution of rosella flower extract with different time variations, ie 1 hour, 2 hours, and 3 hours. Light sources used are sunlight and 150 watt halogen lamps. It was found that at 3 hours of immersion using the sunlight source was the best result of maximally current (Imax) 0.3 mA, maximum voltage (V) 582,4 mV and its efficiency 0,258%. The results obtained were lower when the DSSC was given a halogen light source, i.e. Maximum current (Imax) 0.08 mA, maximal voltage (Vmax) 125,6 mV and its efficiency 0,167%. The greater the intensity of the light illumination the greater the output power of the DSSC.The use of solar panels is still quite expensive in the making, for it needs to be developed solar panels are cheap based DSSC (Dye Sensitized Solar Cell). The use of DSSC is very good developed in Indonesia which is famous for its biological richness. This study used rosella flowers as photosensitizer obtained from the extraction process in the form of maceration for 24 hours. The components used to construct a DSSC outline can be divided into four parts, namely substrate, dye, semiconductor titanium dioxide (TiO2), and electrolyte. In this research the TiO2 layer was immersed in a solution of rosella flower extract with different time variations, ie 1 hour, 2 hours, and 3 hours. Light sources used are sunlight and 150 watt halogen lamps. It was found that at 3 hours of immersion using the sunlight source was the best result of maximally current (Imax) 0.3 mA, maximum voltage (Vmax) 582,4 mV and its efficiency 0,258%. The results obtained were lower when the DSSC was given a halogen light source, i.e. Maximum current (Imax) 0.08 mA, maximal voltage (Vmax) 125,6 mV and its efficiency 0,167%. The greater the intensity of the light illumination the greater the output power of the DSSC.

Pengaruh Konsentrasi Gelatin Tulang Ikan Bandeng (Chanos chans F.) Pada Pembuatan Permen Jelly dari Bunga Rosella (Hisbiscus sabdariffa L.)

Rosella is one of the plants that has many benefits as well as nutrients used potentially as food ingredients such as jelly candy. Jelly candy was made by mixing rosella flower extracts with a gelling agent. This study aimed to determine the effect of the addition of gelatin from milkfish bones toward the production of jelly from rosella flowers and to determine the addition concentration of gelatin from milkfish bones to obtain a good quality of jelly candy. The gelling agent used was gelatin derived from milkfish bones. Gelatin from milkfish bones was made through several stages i.e. degreasing, demineralization, extraction, and drying then applied to produce jelly candy. Rosella flower was extracted using the infusion method at a temperature of 70 oC. The addition concentration of gelatin varied of 10, 12, 14, 16 18, and 20%. Parameters used were water content and organoleptic test covering of color, aroma, flavor, and texture. Results showed that addition of gelatin from milkfish bones influenced strongly on the moisture content, color, aroma, flavor, and texture of the jelly candy. The good quality of rosella jelly candy was obtained by addition concentration of gelatin of 18% with a water content of 8.76%, and the elasticity was preferred by the panelists.

Rosella Flower Extract Prevent Increasing Of Interleukin-6 And Amyloid-Levels In Brain Tissue Of Heated Diet-Treated Rats

Heated food is a source of formation of various types of Advanced Glycation Endproducts (AGEs). One of which is N-carboxymethyl-lysine (CML) that is often used as a sign of the formation of AGEs in the diet. The accumulation of AGEs in the brain can trigger neurodegenerative conditions. It also accelerates the accumulation of Amyloid β and proinflammatory cytokines which exacerbate neurodegeneration. Hibiscus sabdariffa L or rosella known to have antiglycation, antioxidant, and anti-inflammatory effects. This study aims to prove the effect of food heating to the increasing levels of CML serum and evaluate the effects of daily intake of Rosella extract IL-6 levels and Amyloid β in the Wistar rats’ brain given a diet high of AGEs. This experimental study with post test only control group design was conducted using 25 white male rats Wistar strain ( Rattus novergicus) which were divided into 5 groups namely positive control, negative control, the treatment group with rosella dose 200mg.kgBW-1, 300mg.kgBW-1, and 400mg.kgBW-1. The high diets of AGEs are given for 12 weeks, and it was added to the rosella extract with various doses on the 9thto 12th weeks. The examination of CML serum level, interleukin 6 brain level, and Amyloid β of the brain was carried by ELISA. This study found differences between CML serum level of rats which fed a standard diet and the one that was given a heated diet (p= 0.0001). It also found that rosella flower extract can prevent the increasing levels of interleukin 6 and Amyloid β in the brain tissue of rats which were given a heated diet, and both effective doses were on 200mg.kgBW-1. Keywords : Advanced Glycation End products, Amyloid β, Interleukin 6, N-carboxymethyl-lysine, Rosella.

Preparation and Evaluation of Biscuits Supplemented with some Natural Additives for Children and Adolescents Feeding

To prepare and evaluate a healthy biscuit for reducing malnutrition diseases such as anemia and osteoporosis. Several models of two formulas were proposed by using five types of natural supplements; pumpkin (P), yellow corn germ (G), white kidney bean (Wb), rice bran (R) and rosella flower (K) to supplement wheat flour 72% extraction (W). The results of the sensory evaluation showed that the biscuits supplemented from the following mixtures: PG (80%W+10%P+10%G), PR (80%W+10%P+10%R), PWb (80%W+10% P +10%Wb), WbG (80%W +10%Wb+10%G) and WbR (80%W+10%Wb+10%R)as a duple mixes or T3 (75%W+10%R+5%Wb+5%G+3%P+2%K) as a multi mix had a very good degree of preference, so they were selected to be a chemically and physically evaluate and study the storage stability of the produced biscuits. The results of the chemical analysis indicated that all types of supplemented biscuit had high content of protein, fat, ash, crude fiber and total calories. They have a high mineral content (i.e., iron, zinc, calcium, manganese and magnesium), vitamins contents (i.e., A, E, K, B1, B2, B3, B6 and B9), total essential amino acids, biological value, protein efficiency ratio, total unsaturated fatty acids compared with the control. The color properties of the samples containing the pumpkin were improved. The spread factor and hardness of all types of supplemented biscuits was higher than those for the control. All types of supplemented biscuits were the highest in contribute of most of Recommended Dietary Allowances for a studied previous nutrient for children and adults. So it could be recommended to incorporate the investigated nutritional sources in bakery products to obtain healthy bakery products having high biological values, especially for resistance to anemia and osteoporosis due to the high content of the required nutrients.

Pengaruh Penambahan Kelopak Bunga Rosella Pada Pembuatan Fruit Leather Dari Buah Mangga

Fruits are not always consumed in fresh form. But most are processed into various forms and types of food. This treatment aims in addition to extending the shelf life, as well as to increase the variety of products. Fruits are generally processed into preparations such as jam, jelly, jam, puree, fruit juice, canned fruit, candied dry or wet. One type of dried fruit products other than candied fruits is Fruit leather. Fruit leather is a type of food of fruit flesh that has been destroyed and dried. Drying can be done with drying or it could be with a warming that has a hot temperature of 50-60oC. Fruit leather has a shelf life of up to 12 months, when stored at appropriate storage conditions. In Indonesia, Fruit leather is still not commercially produced. Rossela contains some substances that are very important for health. Including grossy peptin, antochanin, gluside hibiscin, and flavonoids that are useful to prevent cancer, control blood pressure, blood circulation and so forth. The fiber content is high enough that plays a role in launching the exhaust system and lower cholesterol levels in the blood (Erianto, 2009). Based on this background then this study was conducted to combine the mango fruit is known to have high nutritional content with rosella flowers that have some substances that are very important for health. Implementation of Research using Factorial Random Design (RAL), with two main factors that is: Factor I: Varieties of mango fruit (M) consisting of 3 levels: M1: Mango shrimp, M2: sweet sweet Mango, M3: Kueni. Factor II: Addition of rosella (%) of mango flesh, consisting of 4 levels: R1: 40%, R2: 60%, R3: 80%, R4: 100%. The study was conducted with 3 replications. The research was conducted from April to May 2017 at the laboratory of Agricultural Product Technology Faculty of Agriculture UISU Medan. The results showed that the best treatment combination was obtained in Kueni mango with 40% rosella flower meat (M3R1). The strong aroma and taste of Kueni produce the fruit leather with the highest organoleptic value of the color scent. Conversely, the higher percentage of rosella addition resulted in organoleptic assessment of flavor, color and flavor decreased.

FORMULASI SEDIAAN LIPSTIK EKSTRAK BUNGA ROSELLA (Hibiscus sabdariffa) SEBAGAI PEWARNA DAN MINYAK ZAITUN (Olive oil) SEBAGAI EMOLIEN

Islam is a beautiful religion and loves beauty. The using of a lipstick is to form a lip in an attractive appearance and to protect the lips from dryness with no side effects. The use of rosella as a dye and olive oil as an emollient in the formulation of the preparations due to the red pigments of anthocyanin in rosella (Hisbiscus sabdariffa l.) and olive oil can be used as an emollient to keep the moisture of the lips. The purpose of this study was to make a lipstick with a good stability. The simplisia of rosella flowers was macerated in ethanol 96%. The components of the lipstick used are cera alba petroleum jelly, aba, Cetyl alcohol, lanolin, titanium dioxide, nipagin, oleum rosae, olive oil and extracts of flowers rosella with concentrations of 0%, 2%, 4%, 6%, 8%, 10%. The lipstick mixture was then tested including: homogeneity, stability, grease, melting point and pH tests. Test a fondness and irritation test performed on the 25 panelists. The results showed that the concentrations of rosella extract 0% did not cause any color or white, the concentration of 2%-4% color posed red fade, the concentration of 6% was red, the concentration of 8%-10% was dark red. Concentration of the extract of 0%-4% did not experience the color change (stable) in a storage room temperature for 30 days. As for the material of 6%-10% color lipstick changes slowly, due to the occurrence of oxidation on lipstick. The result of fondness and irritation tests are that in the preparation of 1, 2, 3, 4, 5 and 6 does not cause irritation, itching and skin redness on the skin.

The Effects of Rosella Extract (Hibiscus sabdariffa) against the n-carboxymethyl-lysine, NF-κβ, TNF-α in the Rats Heating Food Diets

The food processing by heating can increase the formation of AGEs (Advanced Glycation End Products). AGEs are compounds that formed from a non-enzymatic continuous glycation reaction between proteins and sugar residues. The CML ( N-carboxymethyl-lysine ) is used as a marker for AGEs cause most commonly found in vivo. The bond of AGEs and RAGE (receptor for AGEs) induce various signaling pathways that trigger inflammation and increase oxidative stress. The AGE and RAGE interaction activate the transcription factor of NF-κβ. NF-κβ activates gene transcription to release proinflammatory cytokines such as TNF-α. Anthocyanins are compounds that can prevent the formation of AGEs and muffle the adverse effects of AGEs. Rosella contains anthocyanin such as: delphinidin-3-O-glucoside, delphinidin-3-O-sambubioside, and cyanidin-3-O-sambubioside. This study is to determine whether the daily intake of Rosella extract can reduce the levels of n-carboxymethyl-lysine in serum, expression of NF-κβ and TNF α in the rats fed with heated food. This study applied experimental post test control using Rattus norvegicus Wistar strain. The samples were divided into 5 groups: KN (negative control/fed without heating), KP (positive control/fed heated food but not treated by Rosella extract), R 1 (fed heated food and treated by 200 mg.kg -1 BW Rosella extract), R 2 (fed heated food and treated by 300 mg.kg -1 BW Rosella extract), and R 3 (fed heated food and treated by 400 mg.kg -1 BW Rosella extract). The n -carboxymethyl-lysine levels were measured by using the ELISA, the expression of NF-κβ is analyzed by using immunofluorescence, and expression of TNF - α is observed by immunohistochemistry. There was significantly decreased the levels of n -carboxymethyl-lysine in all groups which were treated by Rosella extracts (R 1 ,R 2 ,R 3 ); p= 0.000, α = 0.05 (p< α). Decreased activation of NF-κβ in all groups which were treated by Rosella extract is significant (p = 0.000), and decreased expression of TNF α in all groups which were treated by Rosella extract is also significant (p = 0.000). Rosella extract can reduce the levels of n-carboxymethyl-lysine , expression of NF-κβ, and TNF α. Keywords: AGEs, anthocyanin, Rosella flower extract, n -carboxymethyl-lysine , NF-κβ, TNF α.

ROSELLE FLOWER (HIBISCUS SABDARIFFA) IN THE TREATMENT OF HYPERTENSION IN POSTPARTUM MOTHERS

Background: Roselle flower (Hibiscus sabdariffa) has been used as a food and herbal drinks, in hot and cold beverages, as an herbal medicine.&#x0D; Objective: To examine the effect of consuming roselle dried flower petals on changes in maternal hypertensive blood pressure during postpartum period.&#x0D; Methods: This was a quasi-experimental study with non-equivalent control group design conducted in 2016. There were 30 samples recruited using consecutive sampling with 15 samples in an intervention group and a control group. Data were analyzed using paired t-test to know the difference of blood pressure value before and after intervention in the intervention and control group, and independent t-test to determine the difference of blood pressure decrease between intervention and control group. Mann-Whitney and Wilcoxon test were also conducted for data analysis.&#x0D; Results: Findings showed that there were statistically significant differences in systolic and diastolic blood pressure values before and after intervention in the intervention and the control group with p-value 0.000 (&lt;0.05) with systolic blood pressure decrease was 5 mmHg and diastolic was 5.33 mmHg. Additionally, there was a significant effect in the duration of healing in the intervention group with p-value 0.000 (&lt;0.05), with the average duration of healing was 2 days.&#x0D; Conclusion: There were significant effects of dried petals of rosella flowers (Hibiscus sabdariffa) brewed with 200 ml of hot water with temperature 90oC in lowering blood pressure, and there were significant differences in systolic and diastolic blood pressure and duration of healing between the intervention group and the control group of mothers who consumed antihypertensive drugs. The results of this study can be applied by midwives in postpartum care with the cases of hypertension. It is expected that this intervention can help the healing process more quickly through a combination of antihypertensive medicines and sedated petals of rosella flowers.

Inhibition of Toothpaste Denture Cleanser Rosella Petals Have Stored Several Times on Denture Plaque Formation, Colonies of Bacteria and Candida Albicans

&lt;p class="AbstractContent"&gt;&lt;strong&gt;Objective&lt;/strong&gt;: It is known the benefits of rosella flower petals are able to inhibit the growth of bacteria and C. albicans. In addition, do not change the color and transverse strength dentures plate. Unknown inhibitory power after being stored several times in the form of toothpaste.&lt;/p&gt;&lt;p class="AbstractContent"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; This is a clinical and laboratory experimental research, with pre-and post-test design. The effectiveness of roselle denture cleanser toothpaste was assessed on 5 samples 3, 6 and 9 months after they were created. Plaque score was assessed by the Modified Quigley Hein index, colonization of bacteria and C.albicans were assessed through laboratory observations. Data were analyzed using Wilcoxon and Friedman two ways.&lt;/p&gt;&lt;p class="AbstractContent"&gt;&lt;strong&gt;Result:&lt;/strong&gt; The result show that there were changes in the effectiveness of roselle denture cleanser toothpaste in inhibiting the growth of bacteria and Candida in the 9&lt;sup&gt;th&lt;/sup&gt; month, but no change of effectiveness in inhibiting plaque formation.&lt;/p&gt;&lt;p class="AbstractContent"&gt;&lt;strong&gt;Conclusion&lt;/strong&gt;: It was concluded that the effectiveness of roselle denture toothpaste in inhibiting the growth of bacteria and Candida has decreased; but the same cleanser paste is still effective in inhibiting plaque formation after 9-month storage.&lt;/p&gt;

Rosella Flower Decreases the CML Serum and Liver Inflammation of Rats Given Baked-Food Diet

Advanced Glycation Endproducts (AGEs) or so-called glycotoxin can be triggered by heated food in which the Maillard reaction occurs. One type of glycotoxins is CML. Accumulated N-Carboxymethyl-Lysine (CML) can cause inflammation of organs, e.g. liver. Rosella flowers contain anthocyanin compound that has anti-glycation and antioxidant effects. This study aimed to determine the effect of ethanol extract of Rosella on CML serum level, IL-6 level, and NF-ƙB activation in the liver of mice fed with baked food. This study used post-test design using 25 Wistar rats aged 3-4 months old that were divided into 5 groups, namely negative control group, positive control group, treatment groups given Rosella extract dose of 200mg.kgBW -1 , 300mg.kgBW -1 , and 400mg.kgBW -1 . Baked feed was given for 12 weeks, and Rosella ethanol extract was administered in the 9 th to 12 th week. The examination on CML serum and IL-6 of the liver was using ELISA method. Immunofluorescent staining was used to determine NF-ƙB activation in the liver using a confocal microscope. CML serum is proven to increase significantly (p = 0.000). The effective dose of Rosella flower extract to prevent CML Serum increase is 200 mg.kgBW -1 , whereas a dose of 400 mg.kgBW -1 can decrease IL-6 level and NF-ƙB activation. Ethanol extract of Rosella flower decrease the levels of N-carboxymethyl-lysine serum, IL-6, and NF-ƙB activation in the liver of rats given baked-food diet. Keywords: Advanced Glycation Endproducts (AGEs), Interleukin-6, N-Carboxymethyl-lysine, Rosella.