Rosehip Research Articles

Fatty acid profile and oxidation tests of fat extracted from yogurt using rose hip seed oil [pdf

Background Yogurt is a dairy product with a high nutritional value. However, like all milk products, it contains milk fat and is rich in saturated fatty acids. It would be desirable to enrich dairy products in poly- unsaturated fatty acids to increase dietary intake amongst consumers and improve their health. Also, some LAB bacteria are able to produce CLA and CLnA isomers from linoleic and linolenic acids. The aim of this study was to investigate the chemical properties and fatty acid profile of yogurt with the addition of 3.5% of rose hip seed oil. Methods Yogurt was made from skimmed milk and yogurt starter culture YC-180 Ch. Hansen (Denmark), with the addition of 3.5% of rose hip seed oil. The peroxide value, acid value, iodine value, TBA rate and fatty acid profile were determined in fat extracted from the yogurt after 1 and 14 days of storage and in fresh rose hip seed oil. The fatty acid profile was determined using gas chromatographic methods with mass spectrometric detectors. Results Fat extracted from the yogurts had lower levels of peroxides than the fresh oil. It was more acidic and the iodine value was higher than in the fresh oil. Rose hip seed oil enriched the product with polyunsaturated fatty acids. After 14 days of storage, linoleic and linolenic acid levels had increased. Moreover, the content of myristic and palmitic acids had decreased. Conclusions The rose hip seed oil added to the yogurt was less susceptible to oxidation. The content of un- saturated fatty acids in the yogurt increased with the addition of the oil, making yogurt with rose hip seed oil an excellent source of O-3 and O-6 fatty acids. Conjugated linoleic (CLA) and linolenic (CLnA) acids were not detected. However, yogurt manufactured with appropriate adjunct cultures and with the correct oil addition could be a natural source of CLA and CLnA in the human diet.

Determination of Parasitoids and its Parasitoids Life of Diplolepis fructuum (Rübsaamen) (Hymenoptera: Cynipoidea) as Pest of Rosehip (Rosa canina) in Sivas

Bu calisma kusburnu bitkisinde gal olusturan Diplolepis fructuum’ un (Hymenoptera: Cynipoidea)'un parazitoitleri ve bu parazitoitlerin, parazitoitlik durumlarini belirlemek icin yapilmistir. Calisma sonucu parazitoit olarak Chalcidoidea (Hymenoptera) ustfamilyasina ait 13 tur tespit edilmistir. Parazitoitlerin teshis edilmesinde iki yol izlenmistir. Bunlardan ilki ergin ve larva uzerinden morfolojik incelemelerle yapilan teshistir. Ikincisi morfolojik olarak degerlendirilen ergin ve larvalarinin genomik DNA izolasyonu yapilip, cekirdek (ITS2) ve mitokondri (COI) genomlarina ait belirtecler dizilenerek dizi bilgileri karsilastirilmistir. Ayrica acilan gallerin incelenmesi sonucu parazitoitlerin tamaminin ektoparazitoit yasam stratejisine sahip olduklari tespit edilmistir.

Characterization of biochemical traits of dog rose (Rosa canina L.) ecotypes in the central part of Iran

Dog rose (Rosa canina L.) is a wild native species in Iran, with a significant genetic diversity. This plant serves as a rich source of vitamin C, anthocyanins, phenolic contents and carotenoids. Rose hips have been used in several food products, as well as perfumery and cosmetics industries. In this research, we investigate biochemical characteristics of five dog rose ecotypes (Kopehjamshid, Zarneh, Miyankish, Aghcheh and Sadeghiyeh), that were collected from the central part of Iran (Isfahan province). Amounts of vitamin C, total carotenoids, total phenolic contents, total anthocyanins, macro and micro minerals were measured. Seed oil are extracted by soxhlet method and analysed by gas chromatography. The macro and micro minerals levels in the fruit vary significantly among these regions. The results of this study demonstrate that dog rose have great diversity and can be used in breeding programmes in order to increase nutrient values as a food resource additive.

Evaluation of genotoxicity and subchronic toxicity of standardized rose hip extract.

Rose hip is the fruit of the rose plant, which is widely used in food, cosmetics and as a traditional medicine. Therefore, rose hip is considered safe and has a sufficient history of consumption as food. However, few studies have reported on the safety of rose hip extracts in toxicological analyses. Thus, to evaluate the safety of rosehip polyphenol MJ (RHPMJ), an aqueous ethanol extract standardized with the trans-tiliroside content, we performed genotoxicity and 90-day repeated oral dose toxicity studies in compliance with the Organisation for Economic Co-operation and Development-Good Laboratory Practice. RHPMJ did not induce gene mutations in reverse mutation tests of Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2 uvrA strains and did not induce chromosomal aberrations in cultured Chinese hamster lung (CHL/IU) cells. Moreover, micronucleus tests using rat bone marrow showed RHPMJ had no micronucleus-inducing potential. Finally, 90-day repeated oral dose toxicity studies (100-1000 mg/kg) in male and female rats showed no treatment-related toxicity in rats. These data indicate that the RHPMJ had no genotoxicity and a no-observed-adverse-effect level greater than 1000 mg/kg in rats.

Innovative biocapped CuO nano-photocatalysts: a rapid and green method for photocatalytic degradation of 4-nitrophenol

In this work, the rapid extracellular biofabrication of CuO nanoparticles (CONPs) was performed through microwave irradiation (MI) using rosehip extract. Not any hazardous reagents were employed during the fabrication period. The as-synthesized CONPs were characterized using XRD, SEM, EDS, antioxidant activity and FT-IR. The CONPs were crystalline and had average sizes about to 20–40 nm according to SEM. This methodology has the advantages of elimination of any chemical surfactants, simple rout and easy work up. The formation mechanism of CONPs was detected, for the first time, by marginal decrease in pH, potentiometric data, XRD and FTIR techniques. Moreover, CONPs as nano-photocatalyst could effectively photodegrade 4-nitrophenol as an environmental pollutant. The degradation rate is about 88% (for 20 ppm of catalyst) in 120 min under Vis irradiation. It demonstrated that the bio-capping had not any retarding effect during photodegradation. This fabrication protocol opens the windows for a large-scale and green approach for generating other metal oxide that are useful as nano-photocatalyst.

Establishing sterilization protocol and shoot induction medium for in vitro regeneration of sweetbriar (Rosa rubiginosa L.)

Rosa rubiginosa L. is popular in Lesotho where rural communities harvest the rosehips for sale to the local companies which process them into various products. There is, however, a challenge in establishing plantations of the species due to deep seed dormancy and so difficulty in germination. In this study, nodal explants of R. rubiginosa L. were sterilized with 20 g·L-1 Ridomil Gold MZ (RGM) and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP). The explants treated with 20 g·L-1 RGM showed resistant against fungal contamination giving 73% survival explants. Moreover, addition of BAP in shoot induction medium increased shoot formation as compared to 29% response recorded on MS without BAP. Response of 100% shoot formation was obtained on MS medium with 2 mg·L-1 BAP. Key words: 6-Benzylaminopurine, nodal explants, ridomil gold mz.

Therapeutic Applications of Rose Hips from Different Rosa Species.

Rosa species, rose hips, are widespread wild plants that have been traditionally used as medicinal compounds for the treatment of a wide variety of diseases. The therapeutic potential of these plants is based on its antioxidant effects caused by or associated with its phytochemical composition, which includes ascorbic acid, phenolic compounds and healthy fatty acids among others. Over the last few years, medicinal interest in rose hips has increased as a consequence of recent research that has studied its potential application as a treatment for several diseases including skin disorders, hepatotoxicity, renal disturbances, diarrhoea, inflammatory disorders, arthritis, diabetes, hyperlipidaemia, obesity and cancer. In this review, the role of different species of Rosa in the prevention of treatment of various disorders related to oxidative stress, is examined, focusing on new therapeutic approaches from a molecular point of view.

Effects of Harvest Times on the Fatty Acids Composition of Rose Hip (Rosa sp.) Seeds

This study was conducted to determine the change of fatty acids ratios of some rose hip species seeds in different harvest times. Seeds of five genotypes belonging to rose hip species (Rosa sp.) were used in the study. The fruits of species were harvested in six different ripening times and analyzed. Total oil analysis was performed for the fifth harvest only, which was determined as the optimal harvest time. As a result; total oil ratio of rose hip seeds varied as 5.22 and 6.62 g/100g respectively for accessions of Rosa dumalis (MR-12 and MR-15), 6.37 g/100g for R. canina (MR-26), 5.00 g/100g for R. dumalis ssp. boissieri (MR-46) and 5.29 g/100g for R. villosa (MR-84). Eleven fatty acids were determined in rose hip seeds. Among these fatty acids linoleic, oleic, linolenic, palmitic and stearic acids respectively had high ratio. Saturated fatty acids ratio (SFAs) was the highest in R. canina (MR-26) and the lowest in R. dumalis (MR-12); monounsaturated fatty acids ratio (MUFAs) was the highest in R. dumalis (MR-12) and the lowest in R. dumalis ssp. boissieri (MR-46); polyunsaturated fatty acids ratio (PUFAs) was the highest in R. dumalis ssp. boissieri (MR-46) and the lowest in R. dumalis (MR-12). Mono and polyunsaturated fatty acid contents obtained in this study was high; the change of fatty acid profile in the studied species in relation to harvest time was significant for some species and insignificant for others. A conclusion was reached that it is important to pay attention to qualitative and quantitative properties of seeds when conducting studies about rose hip improvement.

Determination of the quantitative content of the amount of organic acids and ascorbic acid in different batches of Rosa majalis and Rosa canina raw material

Rose hips are the pharmacopoeial raw material, and its roots have been widely used in folk medicine. Therefore, these roots attracted our attention. The chemical composition of roots of both Rosa species has been poorly studied. The data of literature sources show that roots contain phenolic compounds, organic acids and amino acids. Rose hips are standardized by the quantitative content of the amount of organic acids and ascorbic acid according to the State Pharmacopoeia of the USSR of the XI-th edition. According to the State Pharmacopoeia of Ukraine (SPhU) 2.0 this raw material is standardized by the quantitative content of ascorbic acid. Aim. To study the quantitative content of the amount of organic acids and ascorbic acid in different batches of samples of Rosa majalis and Rosa canina roots. Material and methods. Roots of Rosa majalis and Rosa canina collected in different regions of Ukraine during 2014-2016 were used for research. The quantitative content of the amount of organic acids was studied by the titration method. Ascorbic acid was determined by the spectrophotometric method. Results and discussion. As a result of studying the quantitative content of the amount of organic acids it was determined that the content of these substances in the raw material ranged from 3 % to 5 % in the roots of Rosa majalis and from 3 % to 4.5 % in the roots of Rosa canina. The content of ascorbic acid in roots of Rosa majalis and Rosa canina fluctuated insignificantly from 0.11 % to 0.13 %. Conclusions. For the first time the quantitative content of the amount of organic acids and ascorbic acid in Rosa majalis and Rosa canina roots collected during three years in seven different places has been determined. The results of our research show that further studying of Rosa majalis and Rosa canina roots is promising taking into account their possible areas of application.

Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice

Atherosclerosis is a disease in which atheromatous plaques develop inside arteries, leading to reduced or obstructed blood flow that in turn may cause stroke and heart attack. Rose hip is the fruit of plants of the genus Rosa, belonging to the Rosaceae family, and it is rich in antioxidants with high amounts of ascorbic acid and phenolic compounds. Several studies have shown that fruits, seeds and roots of these plants exert antidiabetic, antiobesity and cholesterol-lowering effects in rodents as well as humans. The aim of this study was to elucidate the mechanisms by which rose hip lowers plasma cholesterol and to evaluate its effects on atherosclerotic plaque formation. ApoE-null mice were fed either an HFD (CTR) or HFD with rose hip supplementation (RH) for 24 weeks. At the end of the study, we found that blood pressure and atherosclerotic plaques, together with oxidized LDL, total cholesterol and fibrinogen levels were markedly reduced in the RH group. Fecal cholesterol content, liver expression of Ldlr and selected reverse cholesterol transport (RCT) genes such as Abca1, Abcg1 and Scarb1 were significantly increased upon RH feeding. In the aorta, the scavenger receptor Cd36 and the proinflammatory Il1β genes were markedly down-regulated compared to the CTR mice. Finally, we found that RH increased nitric oxide-mediated dilation of the caudal artery. Taken together, these results suggest that rose hip is a suitable dietary supplement for preventing atherosclerotic plaques formation by modulating systemic blood pressure and the expression of RCT and inflammatory genes.

Healing incisional surgical wounds using Rose Hip oil in rats

Purpose: To evaluate incisional surgical wound healing in rats by using Rose Hip (Rosa rubiginosa L.) oil.
 Methods: Twenty-one days after the oophorectomy procedure, twenty-seven female, adult, Wistar rats were distributed into three groups: Control group (wound treatment with distilled water); Collagenase group (treatment with collagenase ointment); and Rose Hip group (wound treatment with Rose Hip oil). Each group was distributed according to the date of euthanasia: 7, 14 and 21 days. The wound was evaluated considering the macroscopic and microscopic parameters.
 Results: The results indicated differences in the healing of incisional wounds between treatments when compared to control group. Accelerated wound healing was observed in the group treated with Rose Hip oil in comparison to the control and collagenase, especially after the 14th day. Morphometric data confirmed the structural findings.
 Conclusion: There was significant effect in topical application of Rose Hip oil on incisional surgical wound healing.

Anti-prediabetic effect of rose hip (Rosa canina) extract in spontaneously diabetic Torii rats.

Prediabetes, a high-risk state for developing diabetes showing impaired glucose tolerance but a normal fasting blood glucose level, has an increasing prevalence worldwide. However, no study investigating the prevention of impaired glucose tolerance at the prediabetic stage by anti-diabetic functional foods has been reported. Thus, the present study aimed to evaluate the anti-prediabetic effect of rose hip in a prediabetic rat model. Spontaneously diabetic Torii (SDT) rats were supplemented with hot-water extract of rose hip at a dose of 100 mg kg-1 body weight day-1 for 12 weeks. The results obtained showed that the supplementation of rose hip extract improved impaired glucose tolerance, promoted insulin secretion, preserved pancreatic beta-cell function and suppressed plasma advanced glycation end-products formation of methylglyoxal-derived hydroimidazolone (MG-H1) residue and Nϵ -carboxymethyl-lysine residues (e.g. MG-H1, control: 465.5 ± 43.8 versus rose hip: 59.1 ± 13.0 pmol mg protein-1 , P < 0.05) in SDT rats at the prediabetic stage (12-20 weeks old). The present study provides the first evidence showing that a hot-water extract of rose hip could exert an anti-prediabetic effect in a rat model. © 2017 Society of Chemical Industry.

Some Physico-Chemical Characteristics in Fruits of Rose Hip (Rosa spp.) Genotypes from Bolu Province in Western Part of Turkey

More recently, functional foods or food supplements which can protect humans from oxidative stress and several diseases have attracted worldwide interest. Among functional foods, fruits were given special attention, particularly wild edible fruits (neglected or underutilized fruits). There are various neglected and underutilized fruit tree species grown in Turkey solely. They could be exploited directly as foods, or used to obtain valuable natural compounds and derivatives. Rose hip (Rosa spp.) is one of them. The aim of the study is to determine some fruit characteristics of promising rose hip selections from Bolu province in the western part of Turkey. In the selection study, around 100 wild growing rose hip plants were investigated and among them 9 promising genotypes were selected based on rose hip selection criteria. The fruit mass, fruit flesh ratio, soluble solid content, titratable acidity, total dry matter and vitamin C content of these selected nine genotypes ranged from 1.40-2.77 g, 64.92-82.83%, 24.10-30.50%, 0.04-1.55%, 32.44-56.94% and 332.42-1603.52 mg/100 g respectively. Among the 9 promising genotypes, two genotypes had high, two genotypes had medium and five genotypes had low level of thorn.

The use of rose hips in the technology of alcoholic beverage

The problem of developing beer drinks with added fruits, berries or extract's of plant is very actual problem. The aim of this study was to investigate the possibility of using syrup and rose hips in technology Ales. In the work explored the form of the introduction of rose hips, as well as the stage of application, and the maximum allowable amount of additive. The proportion of syrup in different dose was 20 25 and 30% by weight of malt. Syrup made at the stage of wort boiling with hops. Rose hips were added to the number 15,20 and 25 g/l for 5 minutes before the end of wort boiling. The influence of introduced additives on the activity of yeast were studied. It is shown that the biomass growth and activity of yeast in all the variants of the experiment are practically the same. The article discusses the effect of different concentrations of rose hip syrup and insertion of the fruit on the fermentation and quality of final beer. The introduction of the investigated concentrations of the syrup does not impact greatly on the course of fermentation, physico-chemical parameters and organoleptic characteristics of the beverage. Samples with the addition of syrup to the basic characteristics are similar to control sample. They are distinguished caramel taste, aggravated by the increasing share of the syrup. It is shown that the optimal form to make the herbal supplement of rose hips in the technology of production of Ales are rose hips. The most balanced organoleptic characteristics was the sample with the rose hips in a quantity of 0,4 kg on 1 dal Ale.

Rose hip supplementation increases energy expenditure and induces browning of white adipose tissue

BackgroundOverweight and obesity are widespread chronic disorders defined as excessive fat accumulation, and are major risk factors for several chronic diseases including type 2 diabetes, coronary heart disease, high blood pressure and fatty liver. Changes in lifestyle such as increased physical activity and a healthy diet can be crucial tools for treating obesity. Intake of rose hip, the fruit of several plants belonging to the Rosaceae family, has been shown to reduce body fat mass and prevent body weight gain. Thus, the aim of the study was to elucidate potential mechanisms through which rose hip inhibit diet-induced obesity.MethodsC57BL/6 J mice were fed a high fat diet with (RH) or without (CTR) rose hip supplementation for three months. In vivo indirect calorimetry was monitored, as well as gene expression and protein levels of different adipose depots.ResultsAlthough no differences in energy intake were found compared to the CTR group, RH prevented body weight gain and lowered blood glucose, insulin and cholesterol levels. Indirect calorimetry showed that RH-fed mice have significantly higher EE during the dark phase, despite comparable voluntary activity. Moreover, when challenged with treadmill running, RH-fed mice exhibited higher metabolic rate. Therefore, we hypothesized that RH could stimulate the brown adipose tissue (BAT) thermogenic capacity or may induce browning of the white adipose tissue (WAT). Compared to the CTR group, gene expression and protein levels of some brown and “brite” markers, together with genes able to promote brown adipocyte differentiation and thermogenesis (such as ucp1, tbx15, bmp7, and cidea), as well as phosphorylation of AMPK, was increased in WAT (but not in BAT) of RH-fed mice.ConclusionsTaken together these results indicate that dietary rose hip prevents body weight gain by increasing whole body EE and inducing browning of WAT. Thus, it has potential therapeutic implication for treatment of obesity and related metabolic disorders.

Effects of dietary administration of Rose hip and Safflower on growth performance, haematological, biochemical parameters and innate immune response of Beluga, Huso huso (Linnaeus, 1758)

The aim of this study was to investigate effects of two dietary medicinal herbs, Rose hip (Rosa canina) and Safflower (Carthamus tinctorius) supplementation on growth performance, haematological, biochemical parameters and innate immune response of in juvenile beluga, Huso huso. Fish (26.3 ± 0.4 g) were allocated into 15 tanks (20 fish per tank) and triplicate groups were fed a control diet or diets containing 1% and 2% of medicinal herbs, respectively. Feed conversion ratio (FCR), specific growth rate (SGR) and condition factor (CF) did not show significant differences (P > 0.05) in fish given herbal diets. Significant differences were observed in number of white blood cells (WBC) and haemoglobin (Hb) values among the dietary treatments. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were significantly lower in supplemented diet groups compared with the control. Innate immune responses (lysozyme activity and ACH50) were significantly higher in 2% Safflower-fed fish compared with other groups (P < 0.05). These results indicate that medicinal herbs in diets can be considered as a beneficial dietary supplement for improving the physiological parameters and enhance the immune response of Persian sturgeon.

Fruit Growth Characteristics of Rose Hips (Rosa sp)

This study was carried out to determine the fruit growth characteristics of two rose hip (Rosa) species selected from wild population in Tokat region of the Northern Anatolia, Turkey. Shrubs were propagated by cuttings and planted in 2000 at the Research Station of the Horticultural Department of Agricultural Faculty of Gaziosmanpasa University, Tokat, Turkey. Fruit weight, fruit diameter, fruit length, fruit flesh percentage and seed weight of genotypes belonging Rosa dumalis and Rosa jundzillii were investigated in the study. Fruit samples were picked up weekly from full flowering to optimal harvest time for measurement and weighing. As a result, fruit development characteristics of species measured exhibited single sigmoidal curve except seed weights. In general, developing of measured properties were fast until last 3-4 weeks, then became stable or slightly decreased. Results obtained in 2011 were higher than the results obtained in 2010 in both genotypes.

Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of methylesterification (62%) and acetylation (10%) and consists of different molecular weight populations in the range of 10–100kDa. Enzymatic fingerprinting was performed using a combination of pectin lyase (PL) and endo-polygalacturonase. Detailed information about the structure and level of galacturonic acid oligomers released was obtained using LC-HILIC-MS/ELSD and HPAEC. Predominantly, unsaturated and methyl-esterified oligomers (DP 3–5) were released indicating that high proportions of methylesterified ‘PL degradable’ areas were present within the pectin. The data revealed that homogalacturonan is the main building block of the extracted pectin and consists of long methylesterified/acetylated GalA sequences interspersed with small blocks of non-methyl-esterified GalA units.

Determination of free and esterified carotenoid composition in rose hip fruit by HPLC-DAD-APCI+-MS

Rose hip fruit, which contains high concentration of carotenoids is commonly used for different food products in Europe and it is considered to have medical properties. In this study, a simple, rapid and efficient HPLC-DAD-APCI+-MS method was developed and applied to identify and quantify the carotenoids in rose hip fruit of four rose species, including both unsaponified and saponified extract. In the unsaponified extract 23 carotenoid esters were detected, in which either rubixanthin ester or violaxanthin ester was the dominant component of the ester composition. In the saponified extract 21 carotenoids, including 11 xanthophylls and 10 carotenes were detected. This is the first time the total carotenoid composition, including the carotenoid esters in rose hip fruit were identified and quantified. This work reveals the potential of rose hip fruit to be utilized as a healthy dietary material and give chemical information for the possible future development in the pharmacology field.

Characterization of bioactive compounds in rosehip species from East Anatolia region of Turkey

The objective of this work was to determine some bioactive compounds for four different rosehip species (Rosa L.), growing in the East Anatolia region of Turkey. The highest total phenolic, L-ascorbic acid contents and the highest total antioxidant capacity were found in R. canina. The total phenolic, total anthocyanin, total dry matter, and L-ascorbic acid contents and the total antioxidant capacity of the rosehip species ranged from 1081–6298 mg/100 g, 2.43–3.72 mg/100 g, 40.1–56.7%, 24.98–754.48 mg/100 g, and 10.04–97.95 mmol of Trolox equivalent/g (TE/g), respectively. The major organic acid in the rosehip species was found to be citric acid (0.48–1.05 g/100 g).