Determination of the quantitative content of the amount of organic acids and ascorbic acid in different batches of Rosa majalis and Rosa canina raw material

Abstract

Rose hips are the pharmacopoeial raw material, and its roots have been widely used in folk medicine. Therefore, these roots attracted our attention. The chemical composition of roots of both Rosa species has been poorly studied. The data of literature sources show that roots contain phenolic compounds, organic acids and amino acids. Rose hips are standardized by the quantitative content of the amount of organic acids and ascorbic acid according to the State Pharmacopoeia of the USSR of the XI-th edition. According to the State Pharmacopoeia of Ukraine (SPhU) 2.0 this raw material is standardized by the quantitative content of ascorbic acid. Aim. To study the quantitative content of the amount of organic acids and ascorbic acid in different batches of samples of Rosa majalis and Rosa canina roots. Material and methods. Roots of Rosa majalis and Rosa canina collected in different regions of Ukraine during 2014-2016 were used for research. The quantitative content of the amount of organic acids was studied by the titration method. Ascorbic acid was determined by the spectrophotometric method. Results and discussion. As a result of studying the quantitative content of the amount of organic acids it was determined that the content of these substances in the raw material ranged from 3 % to 5 % in the roots of Rosa majalis and from 3 % to 4.5 % in the roots of Rosa canina. The content of ascorbic acid in roots of Rosa majalis and Rosa canina fluctuated insignificantly from 0.11 % to 0.13 %. Conclusions. For the first time the quantitative content of the amount of organic acids and ascorbic acid in Rosa majalis and Rosa canina roots collected during three years in seven different places has been determined. The results of our research show that further studying of Rosa majalis and Rosa canina roots is promising taking into account their possible areas of application.