Rosa Species Research Articles

Study of the Chemical Composition of Rosa beggeriana Schrenk's Fruits and Leaves.

Rosa species are widely used in folk medicine in different countries of Asia and Europe, but not all species are studied in-depth. For instance, Rosa beggeriana Schrenk, a plant which grows in Central Asia, Iran, and some parts of China, is little described in articles. Column and thin-layer chromatography methods were used to isolate biologically active substances. From a study of fruits and leaves of Rosa beggeriana Schrenk, a large number of compounds were identified, seven of which were isolated: 3β,23-dihydroxyurs-12-ene (1), β-sitosterol (2), betulin (3), (+)-catechin (4), lupeol (5), ethyl linoleate (6), and ethyl linolenoate (7). Their structures were elucidated by 1H, DEPT and 13C NMR spectroscopy, mass spectrometry, and GC-MS (gas chromatography-mass spectrometry). The study also identified the structures of organic compounds, including volatile esters and acids. Consequently, comprehensive data were acquired concerning the chemical constitution of said botanical specimen.

MOLECULAR CLASSIFICATION OF THE GENUS ROSA L. (ROSACEAE) GROWN IN NORTHERN IRAQ BY USING RAPD MARKERS

The presented research, comprising characterization of nine species of the wild rose (Rosa L.), came from various regions of Northern Iraq. The study proceeded in 2021–2022 at the College of Education of Pure Science, University of Mosul, Mosul, Iraq. Employing the random amplification polymorphism DNA (RAPD) helped determine the genetic variation relationships among the species using the statistical program Numerical Taxonomy and Multivariate Analysis System (NTSYSpc 2.02). The use of 10 random primers attained amplifications observed with agarose gel electrophoresis. The RAPD primers generated 523 random bands, making it possible to separate Rosa species from each other. Among the studied species, the genetic distance ranged from 0.067 to 1.027. The least genetic dimension (0.067) came from the species Rosa canina var. deseglisei and Rosa canina var. canina, with the highest genetic breadth reaching 1.027 between Rosa x centifolia and Rosa foetida. The dendrogram revealed three main clusters based on the genetic distance values, with the third one getting split into three distinct groups. RAPD proved as an effective method for studying the relatedness among the species.

Seed Germination within Genus Rosa: The Complexity of the Process and Influencing Factors

Seed germination is a crucial stage in the life cycle of plants, and understanding the factors influencing germination is essential for successful cultivation, plant breeding, and conservation efforts. The genus Rosa, commonly known as roses, encompasses a diverse group of flowering plants renowned for their beauty and fragrance. Rosa germination is influenced by a variety of factors, including seed dormancy, environmental conditions, and seed treatments. Many Rosa species exhibit different types of seed dormancy, such as physical dormancy caused by hard seed coats and physiological dormancy due to internal mechanisms. Overcoming seed dormancy often requires specific treatments, including cold stratification, scarification, or chemical treatments, to promote germination. Environmental factors, including temperature, moisture, light, and substrate, play vital roles in Rosa germination. Temperatures ranging from 15 to 25 °C, moisture, and exposure to light or darkness, depending on the species, constitute suitable conditions for seed germination. Many studies have been conducted to investigate the germination requirements of different Rosa species, thereby expanding our understanding of their propagation and conservation. Additionally, advancements in techniques such as in vitro germination and molecular approaches have further enhanced our understanding of Rosa germination biology.

Otiorhynchus armadillo (Rossi, 1792) (Coleoptera: Curculionidae): a new record and a new pest in Anatolian part of Turkey

With the present study Otiorhynchus armadillo (Rossi, 1792), which is one of several noxious species in Europe, and is first recorded from Anatolia (the Asian part of Turkey) as well as the Asian Continent. The species was recently recorded from the European part of İstanbul. Important morphological features were noted, habitus, aedeagus and feeding damages of the adults on some host plants were photographed. Distribution and damage potential of this species are discussed. Otiorhynchus armadillo can be considered an important ornamental plant pest, particularly on Rosa species in the country. Unfortunately, it has a big potential being a destructive pest for the rose growing area in Isparta, which is also known as “Rose Garden of the World”.

The Importance of Rose and Rosehip Species in Past, Present, and Tomorrow

Rose and rosehip species have been important to people in different civilizations since the historical period. There are many natural and cultural Rosa species in Anatolia, which is an important area for Turks and has been a homeland for many years. In the study, information about the use of these species in the historical process, nutritional values, mythology and current developments were obtained. In addition, today, recommendations for these special species have been determined.

Comparative analysis of the chloroplast genomes of Rosa species and RNA editing analysis

BackgroundThe genus Rosa (Rosaceae) contains approximately 200 species, most of which have high ecological and economic values. Chloroplast genome sequences are important for studying species differentiation, phylogeny, and RNA editing.ResultsIn this study, the chloroplast genomes of three Rosa species, Rosa hybrida, Rosa acicularis, and Rosa rubiginosa, were assembled and compared with other reported Rosa chloroplast genomes. To investigate the RNA editing sites in R. hybrida (commercial rose cultivar), we mapped RNA-sequencing data to the chloroplast genome and analyzed their post-transcriptional features. Rosa chloroplast genomes presented a quadripartite structure and had highly conserved gene order and gene content. We identified four mutation hotspots (ycf3-trnS, trnT-trnL, psbE-petL, and ycf1) as candidate molecular markers for differentiation in the Rosa species. Additionally, 22 chloroplast genomic fragments with a total length of 6,192 bp and > 90% sequence similarity with their counterparts were identified in the mitochondrial genome, representing 3.96% of the chloroplast genome. Phylogenetic analysis including all sections and all subgenera revealed that the earliest divergence in the chloroplast phylogeny roughly distinguished species of sections Pimpinellifoliae and Rosa and subgenera Hulthemia. Moreover, DNA- and RNA-sequencing data revealed 19 RNA editing sites, including three synonymous and 16 nonsynonymous, in the chloroplast genome of R. hybrida that were distributed among 13 genes.ConclusionsThe genome structure and gene content of Rosa chloroplast genomes are similar across various species. Phylogenetic analysis based on the Rosa chloroplast genomes has high resolution. Additionally, a total of 19 RNA editing sites were validated by RNA-Seq mapping in R. hybrida. The results provide valuable information for RNA editing and evolutionary studies of Rosa and a basis for further studies on genomic breeding of Rosa species.

The first chloroplast sequence of Rosa davurica Pall. var. Davurica

Rosa davurica Pall. var. davurica is a member of the plant family Rosaceae. Although R. davurica has high application value, its chloroplast genome sequence has not been reported. This study aims to reveal the genetic characteristics of the chloroplast genome of Rosa roxburghii. The length of its total chloroplast DNA is 156,971 bp, with 37.22% G/C content. Its chloroplast genome has two inverted repeat (IRa and IRb) regions totaling 26,051 bp which are separated by a large single copy (LSC) region of 86,032 bp and a small single copy (SSC) region of 18,837 bp. The genome contains 131 independent genes (86 protein-coding, 37 tRNA, and 8 rRNA), and there are 18 repeated genes within the IR region. Among these genes, 17 genes contained one or two introns. The phylogenetic analysis showed that R. davurica was relatively close to other Rosa species, such as the Rosa hybrid.

Genetic Structure and Selection Signature in Flora Scent of Roses by Whole Genome Re-Sequencing

Roses are important plants, and they are cultivated worldwide for their beautiful flowers, remarkable scent, and nutritious hips. In this study, we re-sequenced the whole genomes of 57 rose cultivars and one wild species that originated from different regions around the world and had different scents, aiming to evaluate their genetic structure and to detect the potential signature of the selective sweep between different scent groups with single-nucleotide polymorphism (SNP) and indel markers. The roses were sequenced at an average depth of 6× to the reference genome of Rosa ‘Old blush’. A total of 2,375,949 SNPs were obtained. The SNP numbers varied among the 58 samples, with an average of 1,271,906 per sample. The phylogeny and population structure revealed that the roses could be divided into three main clusters; however, the groups were not consistent in terms of geographic origin or scent classification. These indicated that rose cultivars have a complex genetic background due to the extensive hybridization between cultivated roses or wild rosa species worldwide. A selective sweep analysis was conducted to detect the selection signatures in rose scent traits. A total of 2430 candidate genes were identified in the strong scent groups, and were at the top 5% FST compared to the moderate group. These genes were significantly enriched in the KEGG pathways of tyrosine metabolism, cyanoamino acid metabolism, alpha-linolenic acid metabolism (13 genes), and phenylpropanoid biosynthesis (30 genes). When the low-scent group was used as the control, 2604 candidate genes were identified at the top 5% FST that were significantly enriched in the KEGG pathways of alpha-linolenic acid metabolism (15 genes), and glutathione metabolism (23 genes). We also observed genes enriched in pathways including the linoleic acid metabolism (five genes), diterpenoid biosynthesis (six genes), and monoterpenoid biosynthesis (seven genes), although they were not significant. These implied a positive selection of rose cultivars with a strong fragrance in terms of fatty acid derivatives, terpenoids and benzenoids/phenylpropanoids during rose breeding. Our study establishes a foundation for the further large-scale characterization of rose germplasm, improving the genetic knowledge of the background of roses.

Phenolic Compounds of Rose Hips of Some Rosa Species and Their Hybrids Native Grown in the South-West of Slovenia during a Two-Year Period (2020-2021).

The genus Rosa is very extensive and variable, so it remains very unpredictable and uninvestigated. This also holds true for values of secondary metabolites in rose hips, which are important for several purposes (human diet, protection of plants against pests, etc.). The aim of our study was to determine the content of phenolic compounds in the hips of R. × R. glauca, R. corymbifera, R. gallica and R. subcanina, which grow wild in nature in southwestern Slovenia. We examined the content of phenolic compounds in different parts of rose hips, in the flesh with skin and in the seeds, depending on the individual species, over a period of two years, 2020 and 2021. We also considered the influence of environmental conditions on the content of the mentioned compounds. In both years, the content of phenolic compounds was higher in the flesh with skin than in the seeds. Considering the total content of phenolic compounds in the flesh with skin, R. gallica stands out (15,767.21 mg/kg FW), but the hips of this species accumulated the lowest number of different phenolic compounds. The lowest content of total phenolic compounds (TPC) was found in R. corymbifera, in the year 2021 (3501.38 mg/kg FW). The content of TPC (in both observed years) in the seeds varied between 1263.08 mg/kg FW (R. subcanina) and 3247.89 mg/kg FW (R. × R. glauca). Among the anthocyanins, cyanidin-3-glucoside was determined, which was predominant in R. gallica (28.78 mg/kg FW), and at least was determined in R. subcanina (1.13 mg/kg FW). When comparing the two years of the period (2020-2021), we found that 2021 was more favorable for the formation of phenolic compounds in the seeds, but 2020 in the flesh with skin.

Ethnobotany, Phytochemistry, Pharmacology, and Toxicology of Rosa Sericea a medicinal plant

Plants have majorly been used for medicinal purposes since the Vedic era. Since the dawn of time, flowering or aromatic plants have been playing an important part in humanity. Medicinal Plant-based treatments are currently used by approximately 80% of the world’s population due to their low cost, high availability, fewer side effects, and other factors. One of these plants is Rosa sericea, sometimes called the silky rose. Rosa species is a Rosaceae genus that generates medicinal natural products like polyphenolic and terpenoid compounds, which are utilized in a range of traditional medicines. It is indigenous to some parts of northern India and in some parts of southwest China. Medicinal effects can be found in almost every part of the plant. Fruits and seeds are the parts of plants that are utilized as remedies. Rosa plants are used to cure many diseases like headaches, cancer, liver complications, etc. Many fruits of this genus are highly rich in minerals and vitamins, particularly rich in vitamins A, C, and E, and flavonoids and other bioactive substances are also present. Rosa sericea has been used in various research for its pharmacological properties, there is a gap or lack of knowledge on production methods. The review’s goal is to provide academics, healthcare practitioners, and politicians with a systematic way to organize massive amounts of incomprehensible data so that they may quickly incorporate existing data and make balanced decisions. The findings of this study suggest the extension of traditional medicinal usage of the examined plants for the treatment of all of the diseases indicated above. The current review seeks to incorporate some available information on the ethnobotany, phytological, and ecology of this genus as well as its therapeutic features.

Metabolic profiles, bioactive compounds and antioxidant activity of rosehips from Xinjiang, China

This study aimed to conduct a systematic evaluation of the morphological characteristics, phytochemical components, antioxidant activity, and secondary metabolites of rosehips (Rosa L.) distributed in Xinjiang, China. They have significant differences in morphology and phytochemical composition and darker rosehips had higher phytochemical concentrations than light-colored rosehips. A total of 512 secondary metabolites were identified in five rosehip species using extensively targeted metabolomics, with flavonoid (179) and phenolic acid (146) as the main metabolites. Comparative analysis of metabolites among species showed that YL (R. iliensis) is a potential source of food and drug development. Differentially accumulated metabolites (DAMs) in R. beggeriana (WC) and its variant YL were significantly enriched in the anthocyanin pathway. In addition, the metabolites in R. laxa var. tomurensis (TME) and R. laxa (SH1) were similar but they were significantly different from R. laxa in another floristic region (SH2). These results can expand our understanding of the bioactive components of Rosa species and provide references for improving their utilization, introduction, and breeding.

Diversity of Sporocadaceae (pestalotioid fungi) from Rosa in China.

Rosa (Rosaceae) is an important ornamental and medicinal plant genus worldwide, with several species being cultivated in China. Members of Sporocadaceae (pestalotioid fungi) are globally distributed and include endophytes, saprobes but also plant pathogens, infecting a broad range of host plants on which they can cause important plant diseases. Although several Sporocadaceae species were recorded to inhabit Rosa spp., the taxa occurring on Rosa remain largely unresolved. In this study, a total of 295 diseased samples were collected from branches, fruits, leaves and spines of eight Rosa species (R. chinensis, R. helenae, R. laevigata, R. multiflora, R. omeiensis, R. rugosa, R. spinosissima and R. xanthina) in Gansu, Henan, Hunan, Qinghai, Shaanxi Provinces and the Ningxia Autonomous Region of China. Subsequently 126 strains were obtained and identified based on comparisons of DNA sequence data. Based on these results 15 species residing in six genera of Sporocadaceae were delineated, including four known species (Pestalotiopsis chamaeropis, Pes. rhodomyrtus, Sporocadus sorbi and Spo. trimorphus) and 11 new species described here as Monochaetia rosarum, Neopestalotiopsis concentrica, N. subepidermalis, Pestalotiopsis tumida, Seimatosporium centrale, Seim. gracile, Seim. nonappendiculatum, Seim. parvum, Seiridium rosae, Sporocadus brevis, and Spo. spiniger. This study also represents the first report of Pes. chamaeropis, Pes. rhodomyrtus and Spo. sorbi on Rosa. The overall data revealed that Pestalotiopsis was the most prevalent genus, followed by Seimatosporium, while Pes. chamaeropis and Pes. rhodomyrtus were the two most prevalent species. Analysis of Sporocadaceae abundance on Rosa species and plant organs revealed that spines of R. chinensis had the highest species diversity. Citation: Peng C, Crous PW, Jiang N, et al. 2022. Diversity of Sporocadaceae (pestalotioid fungi) from Rosa in China. Persoonia 49: 201-260. https://doi.org/10.3767/persoonia.2022.49.07.

Comparative analysis of the chloroplast genomes of Rosa species and RNA editing analysis

Comparative analysis of the chloroplast genomes of Rosa species and RNA editing analysis

Assessment of genetic diversity among wild rose in Morocco using ISSR and DAMD markers

BackgroundMorocco is considered one of the main biodiversity hotspots in the Mediterranean region and contains various plant species including wild and domestic Rosa. This genus is the most important among cultivated ornamental plants in the world, with a high economic value in cosmetics, pharmaceutical industries, and floriculture. In the present study, genetic diversity among the collected accessions of wild Rosa species in Morocco was assessed using Inter-Simple Sequence Repeat (ISSR) and Directed Amplification of Minisatellites DNA (DAMD) markers. ResultsResults confirmed that both markers used have a good efficiency to assess genetic diversity in wild roses. Ten ISSR and eight DAMD primers amplified 276 and 203 loci, with an average of 27.4 and 25 polymorphic alleles per primer, respectively. The polymorphic information content (PIC) values were 0.34 with ISSR and 0.31 with DAMD. Analysis of molecular variance (AMOVA) showed that genetic variation in wild rose occurs mainly within populations (86%) rather than between populations (14%). The region of Azrou (Middle Atlas of Morocco) is the area that registered the highest genetic diversity in the present study with He = 0.21. The 39 rose accessions were divided into three main groups with among-group similarity of 30%. Principal component analysis and the hierarchical classification were consistent with genetic relationships derived by structure analysis. ConclusionThe findings revealed that the patterns of grouping are weakly correlated with geographical origin. ISSR and DAMD markers showed that the accessions have a good genetic diversity.

Morphological and biochemical diversity in Rosa species

The Rosa L. is one of the widely grown plants in the world and its flowers and fruits has been used in different parts of the world for centuries. Wild grown Rosa plants is one of the most important element of the natural landscape and used as rootstock for roses for centuries as well. In this study the main flower, shrub and fruit characteristics of a large number of Rosa canina L. and Rosa dumalis Bechst. genotypes naturally found in Ardahan province of Turkey has been studied. All plants found very health conditions and indicated their strong defense mechanism of the genera to harsh abiotic and biotic conditions. The majority of genotypes had attractive flowers. The genotypes exhibited fruit mass between 2.81 g to 4.60 g. SSC (soluble solid content), vitamin C, total phenolic, total flavonoid, total carotenoid, and total anthocyanin content of the genotypes ranged from 15.8–20.6%, 502–714 mg per 100 g FW (fresh weight), 405–507 mg gallic acid equivalent per 100 g FW, 1.02–2.00 mg per g FW, 8.40–13.30 mg per g FW and 4.35–6.98 mg cyanidin-3-glucoside equivalent per liter, respectively. Antioxidant activity was determined between 22.3–30.9 mg ascorbic acid equivalent per g fresh weight. Chlorogenic acid was the major phenolic acids in fruits of Rosa ecotypes. Our results indicated promising perspectives for usage of R. canina and R. dumalis fresh fruits studied with considerable levels of bioactive compounds.

A new species of Metopolophium Mordvilko, 1914 (Hemiptera: Aphididae: Aphidinae) on Rosa beggeriana from Iran

A new species of the genus Metopolophium Mordvilko, 1914 (Hemiptera: Aphididae: Aphidinae: Macrosiphini) is described and illustrated. Apterous and alate viviparous females, oviparous females, and apterous males of Metopolophium rogeri sp. nov. on Rosa beggeriana (Rosaceae) are collected from Kerman Province, Iran. The new species is morphologically most similar to M. alpinum Hille Ris Lambers, 1966, but is characterized by the low ratio of processus terminalis to basal segment of antennal segment VI, high ratio of siphunculus to cauda length and the presence of many more accessory setae on the ultimate rostral segment. This holocyclic aphid is monoecious and not attended by ants. The biometric data are given, and the key to the aphids associated with Rosa species in Blackman and Eastop (2021) is modified to accommodate the new species.

In Vitro and Human Pilot Studies of Different Topical Formulations Containing Rosa Species for the Treatment of Psoriasis.

The aim of this study was to evaluate the phytochemical profile and antioxidant properties of the extracts from three Rosa species (R. canina, R. damascena, R. cairo), to develop and investigate topical formulations with lyophilized forms of extracts for the treatment of psoriasis. Phytochemical screening and in vitro total antioxidant capacity (DPPH, FRAP, CUPRAC, SOD) of studied samples were examined and compared. Lyophilized extracts of roses were dissolved in Transcutol HP and different formulations of creams were prepared. Franz diffusion method was used to evaluate the drug release and biocompatibility was tested on HaCaT cells. Rosa damascene had the best results regarding all the analyses that were conducted. After the evaluation of topical products, the formulation with Rosa damascena extract in a self-emulsifying drug delivery system was tested on a human clinical study that involved 20 patients. At the end of the clinical study an improvement in the quality of life of the patients was observed and erythema, induration and scaling were reduced. The present study indicates that our examined extracts exhibited great phenolic content, antioxidant capacity and safety profile of topical formulation and therefore can be used as a reliable source of natural antioxidants and may be used as a complementary treatment to improve the quality life of patients with psoriasis or may be tested on another diseases.

Effect of solvents on the composition of Rosa x damascena concrete oil in multistage solvent extraction

Four different solvents, ethyl acetate, ethanol, petroleum ether, and hexane, were used for the multistage solvent extraction of rose concrete oil from the aromatic plant species of Rosa x damascena. The components present in the concrete oils were analyzed using Gas Chromatography-Mass Spectrometer. After the multistage solvent extraction process, the solvent was removed by using a rotary vacuum evaporator. Methyl alpha d-glucopyranoside, 5-hydroxy methyl furfural, 2,3-butanediol, and ethyl-d glucopyranoside were the major components identified using ethyl acetate ethanol, hexane, and petroleum ether as a solvent, respectively. The phenyl ethyl alcohol and 5-hydroxymethyl furfural were identified as the repeated components in all four solvents. The solvent ethanol showed a different composition when compared to the other three solvents. A high yield was obtained when ethanol was used as a solvent. The type of solvent used significantly impacts the compositions of the concrete oil of Rosa x damascena.

Antioxidant Activity and Flavonoid Estimation in Rosa multiflora and Rosa wichuraiana Fruits and Flowers

The fruits of Rosa multiflora Thunberg and Rosa wichuraiana Crépin are oriental medicine resources used complementary in management dropsy, edema and nocturnal enuresis in Korea. The objective of the present study was to evaluate the antioxidant activity and the content of kaempferol and quercetin of Rosa multiflora and Rosa wichuraiana fruits and flowers. Crude ethanol extracts of the species’ fruits and flowers from the two Rosa species were fractionized with hexane, ether, ethyl acetate and water, and antioxidant activities of the resulting fractions were evaluated in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and superoxide anion radical scavenging activity. The content of kaempferol and quercetin was quantified by high-performance liquid chromatography (HPLC) analyses. The water fraction of R. multiflora and ethyl acetate fraction of R. wichuraiana exhibited the highest DPPH free radical scavenging activity, which are generally proportionally to concentration, and the ethyl acetate fraction of fruit and ether fraction of the flower from the two Rosa species exhibited the highest superoxide anion radical scavenging activity. Meanwhile, the ethyl acetate and ether fraction of flower and fruit from the two Rosa species contained high level content of kaempferol and quercetin. These findings indicate that the antioxidant activity and the content of kaempferol and quercetin of Rosa multiflora and Rosa wichuraiana is dependent on solvent fraction. Moreover, both Rosa species fruits and flowers are promising sources of antioxidant phytochemicals, which further supports their use in complementary oriental medicine resource in Korea.

Functional Compounds and Antioxidant Activity of Rosa Species Grown In Turkey

Rose hips differ from other fruits with their high vitamin C, vitamin E, phenolic, and antioxidant content, making it an economical source of antioxidants. Exploring the fruit and seed components of different Rosa species could enable better use of their potential for various industries. Thus, rose hips of Rosa corymbifera, Rosa rugosa (Thunb.), Rosa alba L., and Rosa canina L. cultivated in the same growing conditions were analyzed. Their antioxidant activity and capacity, vitamin C, total carotenoids and phenolics, tocopherols and seed oils, as well as their fatty acid composition were determined. In addition to having highly polyunsaturated fatty acids, R. canina was also found to have noticeably high antioxidant components. In the overall evaluation (both fruit and oil characteristics), R. canina was found to have the most favorable content, while R. rugosa has the most desirable oil characteristics. As a result of the evaluation of fruit (excluding oil), R. corymbifera and R. canina were determined as prominent species. Despite medium level oil content, R. rugosa can be recommended for seed oil uses. R. corymbifera and R. canina are recommended for the food and food supplement industry. Production of rose hip species that contain the remarkable functional components of fruits and the health-promoting fatty acids of seeds may be used in combination as a marketing tool. In this way, the medicinal plant market share and profitability rate of rose hip will increase.