Root Zone Research Articles

Migration Patterns and Potential Risk Assessment of Trace Elements in the Soil-Plant System in the Production Area of the Chinese Medicinal Herb Scrophularia ningpoensis Hemsl.

Scrophularia ningpoensis Hemsl. holds a prominent place among Chinese medicinal herbs. Assessing the soil-plant system of its origin is crucial for ensuring medication safety. Although some trace elements are essential for the normal functioning of living organisms, exposure to higher concentrations is harmful to humans, so in order to assess the possible health risk of trace elements in the soil-plant system of Scrophularia ningpoensis Hemsl. origin for human assessment, we used non-carcinogenic risk (HI) and carcinogenic risk (CR) for their evaluation. In this paper, the following trace elements were studied in the soil-Scrophularia ningpoensis Hemsl. system: manganese (Mn), iron (Fe), cobalt (Co), zinc (Zn), selenium (Se), molybdenum (Mo), arsenic (As) and lead (Pb). Correlation and structural equation analyses showed that the effect of soil in the root zone on the plant was much greater than the effect of soil in the non-root zone on the plant. The single-factor pollution index (Pi) showed that the soil in the production area of Scrophularia ningpoensis Hemsl. was polluted to a certain extent, notably with Pb showing the highest average Pi values of 0.94 and 0.89 in the non-root and root zones, respectively. Additionally, the Nemerow composite pollution indices (PN) for both zones indicated an alert range. Regarding health risks, exposure to soil in the non-root zone posed higher non-carcinogenic risk (HI) and carcinogenic risk (CR) compared to the root zone, although neither zone presented a significant carcinogenic risk. The potential non-carcinogenic risk (HI) and carcinogenic risk (CR) from consuming Scrophularia ningpoensis Hemsl. leaves and stems were more than ten times higher than that of roots. However, the carcinogenic risk (CR) values for both the soil and plant of interest in the soil- Scrophularia ningpoensis Hemsl. system did not exceed 10-4, and therefore no significant carcinogenic risk existed.

Case study: reducing heating energy consumption in a high tunnel greenhouse with renewable energy and microclimate control by bench-top root-zone heating, bench covers, and under-bench insulation

Appalachian State University's Nexus project designed an efficient greenhouse heating system that integrated renewable energy and root zone heating technology to reduce the greenhouse heating energy burden on local farmers and installed it at local cooperative farms. This study analyzed 5 years of data from 2018 to 2022 to investigate the energy savings and microclimate control effectiveness of the Nexus heating system installed at Springhouse Farm in North Carolina, USA. By varying bench cover materials, bottom insulation, and the number of loops of root zone tubing, the different soil temperatures required for plant types and growth stages were achieved with a single temperature controller. A root zone heating fluid of 32.2 ℃ satisfactorily maintained the germination soil between 20 and 25 ℃ in March 2019 with an average outside temperature of 4.8 ℃ and an average low temperature of − 0.4 ℃. Growing soil maintained an average temperature of 15 ℃ with bottom insulation and an average of 11–12 ℃ without bottom insulation. Compared to the conventional heating system (a forced-air propane unit heater alone), weather-adjusted propane consumption (propane usage divided by heating degree days) was reduced by 65% with the Nexus system alone and 45% with the Nexus system and unit heater together. It shows that the Nexus system has significantly reduced greenhouse heating energy consumption and maintained productive conditions. The renewable energy fraction ranged only 9–13% of the total thermal energy used due to the high inlet temperature entering the solar thermal collector. This can be improved by separating the heat storage and backup heat source.

Enhancing a machine learning model for predicting agricultural drought through feature selection techniques

This study aims to determine the crucial variables for predicting agricultural drought in various climates of Iran by employing feature selection methods. To achieve this, two databases were used, one consisting of ground-based measurements and the other containing six reanalysis products for temperature (T), root zone soil moisture (SM), potential evapotranspiration (PET), and precipitation (P) variables during the 1987–2019 period. The accuracy of the global database data was assessed using statistical criteria in both single- and multi-product approaches for the aforementioned four variables. In addition, five different feature selection methods were employed to select the best single condition indices (SCIs) as input for the support vector regression (SVR) model. The superior multi-products based on time series (SMT) showed increased accuracy for P, T, PET, and SM variables, with an average 47%, 41%, 42%, and 52% reduction in mean absolute error compared to SSP. In hyperarid climate regions, PET condition index was found to have high relative importance with 40% and 36% contributions to SPEI-3 and SPEI-6, respectively. This suggests that PET plays a key role in agricultural drought in hyperarid regions because of very low precipitation. Additionally, the accuracy results of different feature selection methods show that ReliefF outperformed other feature selection methods in agricultural drought modeling. The characteristics of agricultural drought indicate the occurrence of drought in 2017 and 2018 in various climates in Iran, particularly arid and semi-arid climates, with five instances and an average duration of 12 months of drought in humid climates.

Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone.

Chinese cabbage, scientifically known as Brassica rapa subsp. pekinensis, is a highly popular vegetable in China for its delectable taste. However, the occurrence of bacterial soft rot disease poses a significant threat to its growth and overall development. Consequently, this study aimed to explore the defense mechanisms employed by Chinese cabbage against bacterial soft rot disease. Specifically, the investigation focused on understanding the relationship between the disease and the microbial communities present in the soil surrounding the roots of Chinese cabbage. Significant disparities were observed in the composition of microbial communities present in the root-zone soil of healthy Chinese cabbage plants compared to those affected by Pectobacterium brasiliense-caused soft rot disease. The analysis of 16S rRNA gene high-throughput sequencing results revealed a lower abundance of Proteobacteria (8.39%), Acidobacteriot (0.85), Sphingomonas (3.51%), and Vicinamibacteraceae (1.48%), whereas Firmicutes (113.76%), Bacteroidota (8.71%), Chloroflexi (4.89%), Actinobacteriota (1.71%), A4b (15.52%), Vicinamibacterales (1.62%), and Gemmatimonadaceae (1.35%) were more prevalent in healthy plant soils. Similarly, the analysis of ITS gene high-throughput sequencing results indicated a reduced occurrence of Chytridiomycota (23.58%), Basidiomycota (21.80%), Plectosphaerella (86.22%), and Agaricomycetes (22.57%) in healthy soils. In comparison, Mortierellomycota (50.72%), Ascomycota (31.22%), Podospora (485.08%), and Mortierella (51.59%) were more abundant in healthy plant soils. In addition, a total of 15 bacterial strains were isolated from the root-zone soil of diseased Chinese cabbage plants. These isolated strains demonstrated the ability to fix nitrogen (with the exception of ZT20, ZT26, ZT41, ZT45, and ZT61), produce siderophores and indole acetic acid (IAA), and solubilize phosphate. Notably, ZT14 (Citrobacter freundii), ZT33 (Enterobacter cloacae), ZT41 (Myroides odoratimimus), ZT52 (Bacillus paramycoides), ZT58 (Klebsiella pasteurii), ZT45 (Klebsiella aerogenes), and ZT32 (Pseudomonas putida) exhibited significant growth-promoting effects as determined by the plant growth promotion (PGP) tests. Consequently, this investigation not only confirmed the presence of the soft rot pathogen in Chinese cabbage plants in Hangzhou, China, but also advanced our understanding of the defense mechanisms employed by Chinese cabbage to combat soft rot-induced stress. Additionally, it identified promising plant-growth-promoting microbes (PGPMs) that could be utilized in the future to enhance the Chinese cabbage industry.

Effects of different irrigation methods and mulching on yield, growth and water use efficiency of strawberry

ABSTRACT A greenhouse experiment was conducted from 2015–2016 to 2017–2018 on an Aeric Haplaquept to evaluate the effect of irrigation (surface irrigation at furrows at irrigation water: cumulative pan evaporation = 1, drip irrigation to meet 100%, 80% and 60% of actual crop evapotranspiration demand), and mulch (nonwoven jute agro textile, biodegradable plastic film, rice straw and no mulch) regimes on strawberry. Average soil water storage in root zone (86 mm) and fraction of potential plant available water (61%) was highest in jute agrotextile mulched and drip irrigated at 80% actual evapotranspiration. This combination of mulch and irrigation regime increased belowground (root length density, 7.15 × 103 mm−3) and above ground (leaf area index, 9.4) crop growth and emerged as the best option with three- and four-times higher yield (31.1 Mg ha −1) and water use efficiency (23.1 kg m−3), respectively than surface irrigated unmulched strawberry. Results suggest that these findings be incorporated into packages and practices of strawberry cultivation in the moist tropical sub-humid region of Bengal basin and other similar agroecological regions of South Asia.

Wastewater irrigation beneath the water table: analytical model of crop contamination risks

Wastewater irrigation alleviates freshwater scarcity. However, conventional (near)surface irrigation techniques directly expose crops to contaminants. Irrigating wastewater into shallow phreatic zones to raise the water table enhances groundwater evapotranspiration, while using the vadose zone as a bioreactor that attenuates contaminants through dilution, adsorption, and biodegradation. Nevertheless, contaminants may spread across the groundwater, soil, and vegetation. In this study, we focus on the crop contamination risks, and derive a simple analytical model to estimate crop solute uptake. Although crops are not directly exposed to the irrigated wastewater, contaminants (and nutrients) may spread to the root zone. Results show that crop contamination is primarily determined by the root zone water balance, and by solute dispersion and biogeochemical reaction parameters. The model contributes towards identifying hydrogeologically and climatically suitable locations for phreatic zone wastewater irrigation, determining acceptable levels of irrigation water quality, and evaluating crop contamination hazards against the fertigative value of wastewater.

An underground drip water monitoring network to characterize rainfall recharge of groundwater at different geologies, environments, and climates across Australia

Abstract. Understanding when and why groundwater recharge occurs is of fundamental importance for the sustainable use of this essential freshwater resource for humans and ecosystems. However, accurately capturing this component of the water balance is widely acknowledged to be a major challenge. Direct physical measurements identifying when groundwater recharge is occurring are possible by utilizing a sensor network of hydrological loggers deployed in underground spaces located in the vadose zone. Through measurements of water percolating into these spaces from above, we can record the potential groundwater recharge process in action. By using automated sensors, it is possible to precisely determine when recharge occurs (which event, month, or season and for which climate condition). Combined with daily rainfall data, it is possible to quantify the “rainfall recharge threshold”, the amount of rainfall needed to generate groundwater recharge, and its temporal and spatial variability. Australia's National Groundwater Recharge Observing System (NGROS) provides the first dedicated sensor network for observing groundwater recharge at an event scale across a wide range of geologies, environments, and climate types representing a wide range of Australian hydroclimates. Utilizing tunnels, mines, caves, and other subsurface spaces located in the vadose zone, the sensors effectively record “deep drainage”, water that can move beyond the shallow subsurface and root zone to generate groundwater recharge. The NGROS has the temporal resolution to capture individual recharge events, with multiple sensors deployed at each site to constrain the heterogeneity of recharge between different flow paths, and to quantify (including uncertainty bounds) rainfall recharge thresholds. Established in 2022, the network is described here together with examples of data being generated.

Endodermal suberin restricts root leakage of cesium: a suitable tracer for potassium.

An urgent challenge within crop production is to maintain productivity in a world plagued by climate change and its associated plant stresses, such as heat, drought and salinity. A key factor in this endeavor is to understand the dynamics of root suberization, and its role in plant-water relations and nutrient transport. This study focuses on the hypothesis that endodermal suberin, acts as a physical barrier preventing radial potassium (K) movement out of the vascular tissues during translocation. Previous attempts to experimentally support this idea have produced inconsistent results. We developed a Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) method, allowing us to visualize the distribution of mineral elements and track K movement. Cesium (Cs), dosed in optimized concentrations, was found to be an ideal tracer for K, due to its low background and similar chemical/biological properties. In suberin mutants of Arabidopsis thaliana, we observed a positive correlation between suberin levels and K translocation efficiency, indicating that suberin enhances the plant's ability to retain K within the vascular tissues during translocation from root to shoot. In barley (Hordeum vulgare), fully suberized seminal roots maintained higher K concentrations in the stele compared to younger, less suberized root zones. This suggests that suberization increases with root maturity, enhancing the barrier against K leakage. In nodal roots, suberin was scattered towards the phloem in mature root zones. Despite this incomplete suberization, nodal roots still restrict outward K movement, demonstrating that even partial suberin barriers can significantly reduce K loss. Our findings provide evidence that suberin is a barrier to K leakage during root-to-shoot translocation. This understanding is crucial to maintain crop productivity in the face of climate change.

Development and validation of LC-MS/MS method for trace analysis of acrylamide, acrylic acid and N, N-methylene bis acrylamide in sandy loam soil

Acrylamide and N, N-methylene bis acrylamide are most commonly used monomer and crosslinker compounds employed in synthesis of super absorbent hydrogels. When applied as soil conditioners, there are apprehensions that these hydrogels degrade over time and thus may release the toxic monomers in the soil. A method was thus developed using Liquid Chromatography tandem mass spectrometry (LC-MS/MS) for the trace level quantification of acrylamide (AD), acrylic acid (AA) and N,N-methylene-bis-acrylamide (MBA) in sandy loam soil amended by two test hydrogels the Pusa Hydrogel and SPG 1118 hydrogel prepared using AD and MBA. The MRM (multiple reaction monitoring) transitions were optimized for both the compounds. Soil samples were extracted using dispersive solid-phase extraction (dSPE) with a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) technique, employing acetonitrile. All analytes were quantified at trace levels within a five-minute run using UHPLC equipped with a C-18 column.Single laboratory validation of the developed method in soil matrix was conducted based on specificity, linearity, sensitivity, accuracy, precision, matrix effect and measurement of uncertainty. LC-MS/MS exhibited a linear response in the concentration range of 0.001 to 1 µg mL−1, with correlation coefficient >+0.99. Acceptable recovery (within 70–120 %) with repeatability (%RSD ≤20 %) was obtained at 0.01 to 1 µg g–1 fortification levels. LOQ (Limit of quantification) of the method for AD, AA and MBA in soil matrix were 0.05, 1 and 0.01 µg g–1, respectively. Both intra-laboratory repeatability and intermediate precision at LOQ suggested well acceptable precise (HorRat≈ 0.3) method for quantification. Matrix enhancement effect was observed in the order: AA>AD>MBA. The Expanded Uncertainty (EU) in soil matrix at LOQ was 21.64 %, 28 % and 19 % for AD, AA and MBA respectively. Groundnut and wheat grown with application of the hydrogels showed no detectable residues of monomers in soil samples (total n = 60) near the root zone at the time of crop harvesting.

A potential role of heat-moisture couplings in the range expansion of Striga asiatica.

Parasitic weeds in the genera Orobanche, Phelipanche (broomrapes) and Striga (witchweeds) have a devastating impact on food security across much of Africa, Asia and the Mediterranean Basin. Yet, how climatic factors might affect the range expansion of these weeds in the context of global environmental change remains unexplored. We examined satellite-based environmental variables such as surface temperature, root zone soil moisture, and elevation, in relation to parasitic weed distribution and environmental conditions over time, in combination with observational data from the Global Biodiversity Information Facility (GBIF). Our analysis reveals contrasting environmental and altitude preferences in the genera Striga and Orobanche. Asiatic witchweed (Striga asiatica), which infests corn, rice, sorghum, and sugar cane crops, appears to be expanding its range in high elevation habitats. It also shows a significant association with heat-moisture coupling events, the frequency of which is rising in such environments. These results point to geographical shifts in distribution and abundance in parasitic weeds due to climate change.

Phenotyping revealed tolerance traits and genotypes for acidity and aluminum toxicity in European Vicia faba L.

Soil acidity is a global issue; soils with pH <4.5 are widespread in Europe. This acidity adversely affects nutrient availability to plants; pH levels <5.0 lead to aluminum (Al3+) toxicity, a significant problem that hinders root growth and nutrient uptake in faba bean (Vicia faba L.) and its symbiotic relationship with Rhizobium. However, little is known about the specific traits and tolerant genotypes among the European faba beans. This study aimed to identify response traits associated with tolerance to root zone acidity and Al3+ toxicity and potentially tolerant genotypes for future breeding efforts. Germplasm survey was conducted using 165 genotypes in a greenhouse aquaponics system. Data on the root and shoot systems were collected. Subsequently, 12 genotypes were selected for further phenotyping in peat medium, where data on physiological and morphological parameters were recorded along with biochemical responses in four selected genotypes. In the germplasm survey, about 30% of genotypes showed tolerance to acidity and approximately 10% exhibited tolerance to Al3+, while 7% showed tolerance to both. The phenotyping experiment indicated diverse morphological and physiological responses among treatments and genotypes. Acid and Al3+ increased proline concentration. Interaction between genotype and environment was observed for ascorbate peroxidase activity, malondialdehyde, and proline concentrations. Genomic markers associated with acidity and acid+Al3+-toxicity tolerances were identified using GWAS analysis. Four faba bean genotypes with varying levels of tolerance to acidity and Al3+ toxicity were identified.

In situ monitoring of the impacts of Melaleuca styphelioides transpiration on soil water dynamics in an urban environment

Expansive soils are susceptible to substantial cyclical moisture variations under the prevailing climate, experiencing considerable shrinkage during drought and swelling when water availability increases. These dynamic volumetric changes jeopardize the functionality of lightweight infrastructures with shallow foundations. The presence of trees may amplify seasonal soil movements, as extensive water uptake by root networks in the summer months can induce soil moisture depletion and the resulting desiccation-driven settlement around the trees. This study assessed the drying effect of mature Melaleuca styphelioides on soil movement and water dynamics at an urban experimental site in Melbourne, Australia, instrumented for in situ monitoring over 12 months. Sap flow instrumentation was used to quantify seasonal variations in tree transpiration. The monitoring results revealed that the drying influence of the tree has extended to a depth of 2.2 m. Tree root-induced soil desiccation occurred 4.0 m away from the tree, as evidenced by the seasonal soil movement profiles. Linear regression analysis showed that the soil water content explained 60% of the variability in tree transpiration. In contrast, the soil water content and suction exhibited a robust negative correlation (R2=0.96) within the active root zone.

Review of soil form and wetness indicators for wetland delineation in South Africa

Wetland delineation in South Africa incorporates soil form and soil wetness indicators, requiring formal soil classification and description of soil redox morphology. The current wetland definition used administratively in South Africa focuses on saturated (hydric) soil signatures within plant root zones. Saturated soil horizons deeper than plant root zones fall outside the 50 cm criterion in the local approach as well as the accepted zone in USA literature. The field of hydropedology accommodates the classification of the various hydrologically active horizons and provides a tool for the handling of horizons with ephemeral wetness. This approach has been variably accepted by mandated authorities in South Africa. The South African soil classification system has evolved through three editions over the past 50 years while retaining the same redox morphology understanding. However, despite the concepts and context of redox morphology having been thoroughly technically adopted by soil scientists, this is not the case within the wetland research and management environment. This especially because the classification system is structured differently from other international systems, and the South African landscape is geologically ancient with mature soils, introducing challenges to resource assessment specialists who rely on international norms and approaches for wetland assessment. This paper reviews the various components of soil classification and redox morphology based on Fe and Mn minerals within the context of the South African soil classification system, the field of hydropedology and wetland delineation indicators. We provide a qualitative correlation between the various diagnostic horizons and materials in the system and their related redox morphology contexts that are relevant to wetland assessment, delineation, and protection in South Africa. This paper therefore aims to serve as a reference point for the description and correlation of various soil hydrological parameters used in formal assessments.

Sulfur oxidation and reduction are coupled to nitrogen fixation in the roots of the salt marsh foundation plant Spartina alterniflora

Heterotrophic activity, primarily driven by sulfate-reducing prokaryotes, has traditionally been linked to nitrogen fixation in the root zone of coastal marine plants, leaving the role of chemolithoautotrophy in this process unexplored. Here, we show that sulfur oxidation coupled to nitrogen fixation is a previously overlooked process providing nitrogen to coastal marine macrophytes. In this study, we recovered 239 metagenome-assembled genomes from a salt marsh dominated by the foundation plant Spartina alterniflora, including diazotrophic sulfate-reducing and sulfur-oxidizing bacteria. Abundant sulfur-oxidizing bacteria encode and highly express genes for carbon fixation (RuBisCO), nitrogen fixation (nifHDK) and sulfur oxidation (oxidative-dsrAB), especially in roots stressed by sulfidic and reduced sediment conditions. Stressed roots exhibited the highest rates of nitrogen fixation and expression level of sulfur oxidation and sulfate reduction genes. Close relatives of marine symbionts from the Candidatus Thiodiazotropha genus contributed ~30% and ~20% of all sulfur-oxidizing dsrA and nitrogen-fixing nifK transcripts in stressed roots, respectively. Based on these findings, we propose that the symbiosis between S. alterniflora and sulfur-oxidizing bacteria is key to ecosystem functioning of coastal salt marshes.

IMPACT OF SOIL STERILIZATION FROM DIFFERENT LOCATIONS, WHEAT SEEDS CLEANING AND THEIR INTERACTIONS ON WEED CONTROL, AND YIELD AND YIELD COMPONENTS

IMPACT OF SOIL STERILIZATION FROM DIFFERENT LOCATIONS, WHEAT SEEDS CLEANING AND THEIR INTERACTIONS ON WEED CONTROL, AND YIELD AND YIELD COMPONENTS

Elevational Patterns of Forest Evapotranspiration and Its Sensitivity to Climatic Variation in Dryland Mountains

Elevational climatic heterogeneity, complex terrains, and varying subsurface properties affect the sensitivity of evapotranspiration (ET) in dryland mountain forests to hydrometeorological changes. However, the elevational distribution of ET sensitivity and its major influencing factors remain poorly understood. This study focused on the mid-altitude zone (1000–3500 m) forests in the Chinese Western Tianshan Mountains and assessed ET sensitivity to multiple climate variables, including precipitation (P) and potential evapotranspiration (PET), from 2000 to 2020. To evaluate the multi-year mean and trends in ET sensitivity, multi-source remote sensing data and regional survey data were analyzed using Spearman’s correlation coefficient, the sliding window method, and Kendall’s test. Furthermore, the relative importance of environmental variables (topography, geology, soil, and vegetation) was investigated. P and PET showed no significant trends, while ET exhibited a significant increasing trend (5.81 mm/yr, p &lt; 0.01), particularly at elevations above 2000 m. Most forests (93.5%) showed a positive sensitivity of ET to P, and 70.0% showed a positive sensitivity of ET to PET, mainly at elevations of 1500–2500 m. Additionally, the trend in ET sensitivity to P decreased with an increasing elevation, with 64.5% showing a positive trend. Meanwhile, the trend in ET sensitivity to PET increased with elevation, with 88.1% showing a positive trend. Notably, 53.2% of the forests showed increasing ET sensitivity trends to both P and PET, primarily at elevations of 2000–3000 m with a mean normalized difference vegetation index (NDVI) of 0.56. Geological factors, particularly the hydrological properties of weathered bedrock, contributed the most (~47%) to mean sensitivity. However, geological and vegetative factors, including the NDVI and root zone water availability, were the main contributors (35% each) to the sensitivity. This study highlights the elevation-dependent sensitivity of dryland mountain forests to hydrothermal changes, with higher-elevation forests (&gt;2000 m) being more sensitive to global warming.

Сравнително проучване на коренова и почвена микробиологична активност при самостоятелно и съвместно отглеждане на зеле (Вrassicа oleraceae L. var. capitata L.)

A comparative study of root and soil microbiological activity was carried out during independent and co-cultivation of cabbage with other crops: leeks, beans, tagetes, fennel and flower mix. The intercropping of cabbage and flower mix increases the amount of microorganisms in the immediate vicinity of the cabbage roots to the highest degree. The biogenicity of the root zone is the lowest in the variant with cabbage and tagetes. In the independent cultivation of cabbage, a higher value of the total microflora is established compared to the variants with the main cabbage crop and additional tagetes or leek crops. Combining cabbage with flower mix, beans or fennel increased the amount of microorganisms from the rhizoplane and rhizosphere to a higher degree than growing cabbage alone. The main share in the composition of the total microflora is occupied by non-sporeforming bacteria, followed by bacilli. Regrouping was found in the co-cultivation of cabbage and flower mix, and in this variant the amount of spore-forming bacteria was higher than that of non-spore-forming bacteria. Actinomycetes and mold fungi are less represented. Catalase activity was highest in the control, followed by the variant with co-cultivation of cabbage and leek, and lowest in cabbage and flower mix, and cabbage and fennel, depending significantly on the humidity of the variants. Cellulase activity was higher in the variants with crops compared to the control - highest in the co-cultivation of cabbage and leek, and cabbage and tagetes, and lowest in cabbage and beans, depending significantly on the total microflora.

Ground-penetration radar detection of Root-mass in a Tree Groove in Southwestern Nigeria

In this study, we present the results of a ground-penetrating radar (GPR) survey aimed at imaging the lateral root systems of a section of a tree groove lined by the Tectona Grandis (Teak) species. This was to set the basis for deploying a multi-frequency GPR system for the non-invasive monitoring of rootmass development and evaluation of plant development and health. The survey involved a single GPR Transect with a total length of 60.1 m established along the middle of a line of matured Teak trees. Data was collected at 250 MHz, 500 MHz and 1000 MHz, simultaneously using a wheel-triggered Utsi Trivue system. Data processing involved a dewow, static corrections, gain application and background removal. A root zone consisting of two layers was delineated and a total of 159 hyperbolae were interpreted as roots. The roots were limited to the shallow subsurface, the upper 1.1 m of a potential root zone 1.6 m deep. Imaged roots generally occur in clusters that create much disturbance of the otherwise continuous reflections in the root zone. Often, roots may be laterally offset from the location of tree bases on the Transect. We further identified a potential set of laterally migrating roots in a zone of undisturbed ground outside the main groove indicating the lateral reach of the usually shallow roots of the Teak tree. The results set the basis for further work in root-mass estimation and monitoring tree health within our team.

Tissue and cellular localization of condensed tannins in poplar roots and potential association with nitrogen uptake.

Condensed tannins are common in vegetative tissues of woody plants, including in roots. In hybrid poplar (Populus tremula x alba; also known as P. x canescens) CT assays indicated they were most concentrated in younger white roots and at the root tip. Furthermore, CT-specific staining of embedded tissue sections demonstrated accumulation in root cap cells and adjacent epidermal cells, as well as a more sporadic presence in cortex cells. In older, brown roots as well as roots with secondary growth (cork zone), CT concentration was significantly lower. The insoluble fraction of CTs was greatest in the cork zone. To determine if CT accumulation correlates with nutrient uptake in poplar roots, a microelectrode ion flux measurement (MIFE™) system was used to measure flux along the root axis. Greatest NH4 + uptake was measured near the root tip, but NO3- and Ca2+ did not vary along the root length. In agreement with earlier work, providing poplars with ample nitrogen led to higher accumulation of CTs across root zones. To test the functional importance of CTs in roots directly, CT-modified transgenic plants could be important tools.

Effect of high frequency subsurface drip fertigation on plant growth and agronomic nitrogen use efficiency of red cabbage

High emissions of nitrogen to the environment are one of the major drawbacks of modern agriculture. Subsurface drip fertigation (SDF) is a technology to apply fertilizer in small amounts continuously and directly into the root zone with the potential of mitigating deep percolation losses when accurately managed. Our study was established to analyze possible benefits of SDF, i.e. nitrate losses reduction without decrease in yield, as alternative to the conventional application of nitrogen fertilizer. In this five-year field study, effects of SDF on red cabbage (Brassica oleracea) growth, yield, root distribution and nitrogen uptake were evaluated. The experiments took place in northern Germany. Application of fertilizer in a solution with water was managed to match the needs of crops and placed directly in the root zone through permanently buried driplines. The outcomes of crop growth under SDF were compared with a control crop receiving fertilizer at one or two dates by surface broadcasting. Yield and agronomic nitrogen use efficiency were higher for crops grown under SDF. Total dry mass increase was especially high under dry conditions (34%) but as well under wet conditions with efficient management (20%). Head dry mass of crops grown under SDF had a stronger reaction to the available nitrogen than crops under conventional application, with values of 0.19 and 0.1 Mg ha-1 per kg N ha-1 respectively. Moreover, SDF treatment resulted on average ca. 14 kg ha−1 higher nitrogen uptake. In SDF plots, yield formation and nitrogen uptake was higher for plants grown directly above drip lines. Effect of the distance to nitrogen source was supported with numerical simulations. The root structure showed different distributions for the two treatments, particularly in years with low rainfall. Overall, the results showed the potential of SDF to effectively control nitrogen supply, thereby increasing yield formation of marketable plant organs.