In this study, the effects of deficit water (DW) (normal=control, ½ reduced and 3/4 reduced) and some beneficial rhizobacteria (Azospirillum lipoferum, Bacillus megaterium) and microalgae (Chlorella saccharophilia) on some growth and physiological parameters of fenugreek (Trigonella foenum-graecum L.) were investigated. The experiment was carried out in a fully controlled climate cabinet with 3 replications in factorial order according to the Random Plots Trial Design. Fenugreek (Trigonella foenum-graecum L.) plant was used as material. The study aims to investigate the effects of different deficit water treatments (normal=control, ½ reducedand 3/4 reduced) and some rhizobacteria (Control=R0, Azospirillum lipoferum=R1, Bacillus megaterium=R2) and microalgae (Chlorella saccharophilia=R3) on the growth and development of fenugreek. The effects of deficit irrigation practices on stem length, root fresh and dry weight, stem fresh and dry weight, and leaf area were found to be statistically significant. As the deficit irrigation doses increased, all growth parameters decreased. Root length, root age and stem weight were statistically significantly affected in rhizobacteria and microalgae applications. The highest stem length (19.32 cm) and root wet (1.34 g) and dry (0.18 g) weight were obtained from Bacillus megaterium applications.