Root Material Research Articles

Antimicrobial and Mechanical Effects of Zeolite Use in Dental Materials: A Systematic Review.

ObjectiveIon-incorporated zeolite is a widely used antimicrobial material studied for various dental applications. At present, there is no other systematic review that evaluates the effectiveness of zeolite in all dental materials. The purpose of this study was to review all available literature that analyzed the antimicrobial effects and/or mechanical properties of zeolite as a restorative material in dentistry.Material and MethodsFollowing PRISMA guidelines, an exhaustive search of PubMed, Ovid Medline, Scopus, Embase, and the Dentistry & Oral Sciences Source was conducted. No language or time restrictions were used and the study was conducted from June 1, 2020 to August 17, 2020. Only full text articles were selected that pertained to the usage of zeolite in dental materials including composite resin, bonding agents, cements, restorative root material, cavity base material, prosthesis, implants, and endodontics.ResultsAt the beginning of the study, 1534 studies were identified, of which 687 duplicate records were excluded. After screening for the title, abstract, and full texts, 35 articles remained and were included in the qualitative synthesis. An Inter-Rater Reliability (IRR) test, which included a percent user agreement and reliability percent, was conducted for each of the 35 articles chosen.ConclusionAlthough ion-incorporated zeolite may enhance the antimicrobial properties of dental materials, the mechanical properties of some materials, such as MTA and acrylic resin, may be compromised. Therefore, since the decrease in mechanical properties depends on zeolite concentration in the restorative material, it is generally recommended to add 0.2-2% zeolite by weight.

N uptake, assimilation and isotopic fractioning control δ 15N dynamics in plant DNA: A heavy labelling experiment on Brassica napus L.

In last decades, a large body of evidence clarified nitrogen isotope composition (δ15N) patterns in plant leaves, roots and metabolites, showing isotopic fractionation along N uptake and assimilation pathways, in relation to N source and use efficiency, also suggesting 15N depletion in plant DNA. Here we present a manipulative experiment on Brassica napus var. oleracea, where we monitored δ 15N of purified, lyophilized DNA and source leaf and root materials, over a 60-days growth period starting at d 60 after germination, in plants initially supplied with a heavy labelled (δ 15NAir-N2 = 2100 mUr) ammonium nitrate solution covering nutrient requirements for the whole observation period (470 mg N per plant) and controlling for the labelled N species (ṄH4, ṄO3 and both). Dynamics of Isotopic Ratio Mass Spectrometry (IRMS) data for the three treatments showed that: (1) leaf and root δ 15N dynamics strictly depend on the labelled chemical species, with ṄH4, ṄO3 and ṄH4ṄO3 plants initially showing higher, lower and intermediate values, respectively, then converging due to the progressive NH4+ depletion from the nutrient solution; (2) in ṄH4ṄO3, where δ15N was not affected by the labelled chemical species, we did not observe isotopic fractionation associated to inorganic N uptake; (3) δ15N values in roots compared to leaves did not fully support patterns predicted by differences in assimilation rates of NH4+ and NO3-; (4) DNA is depleted in 15N compared to the total N pools of roots and leaves, likely due to enzymatic discrimination during purine biosynthesis. In conclusion, while our experimental setup did not allow to assess the fractionation coefficient (ε) associated to DNA bases biosynthesis, this is the first study specifically reporting on dynamics of specific plant molecular pools such as nucleic acids over a long observation period with a heavy labelling technique.

Lateral sediment erosion with and without the non-dense root-mat forming seagrass Enhalus acoroides

Coastal areas are at increasing risk from flooding and erosion due to coastal development and climate change. Seagrass meadows, like other coastal ecosystems, can stabilise sediments, indirectly reducing coastal erosion. There is evidence that seagrass can reduce both the erosion of seabeds and lateral erosion of seagrass cliffs. However, these previous studies have only considered seagrass species whose below-ground biomass form dense root mats. This study aimed to investigate whether Enhalus acoroides, a species which does not form root mats, reduces lateral erosion rates of seagrass cliffs. To do this, samples of E. acoroides collected in sandy sediments within Xincun Bay China were transplanted into a wave flume. Lateral erosion rates were measured under exposure to waves for five cliff heights over 1 h. There was an interaction between cliff height and time on the rate of erosion, with higher erosion rates earlier in the experiment for larger cliff heights. The cores with the E. acoroides persisted longer than unvegetated cores, regardless of cliff height, but this result was not assessed statistically due to constraints in the analyses. The below-ground biomass of E. acoroides samples in this study was higher than values previously reported in the literature. Overall, these results suggest that non-dense root-mat forming seagrass species may not provide equivalent sediment stabilisation as dense root-mat-forming species, likely due to the reduced binding structure of the roots in species such as Enhalus.

Comparative evaluation of dentin volume removal and centralization of the root canal after shaping with the ProTaper Universal, ProTaper Gold, and One-Curve instruments using micro-CT.

Background. The main goal of our study was to assess the volume of dentin removed and transportation in root canals using ProTaper Universal (PTU), ProTaper Gold (PTG) and One-Curve (OC). Ni-Ti rotary instruments in extracted human teeth using by micro-CT. Methods: Thirty human upper 1st premolar teeth with two separate root canals and sturdy, mature root tips were used in the present study. Specimens were decoronated and root length was standardized for micro CT scanning before root canal preparation done. The teeth were randomly separated into three categories (n = 10) according to the rotary NiTi system used for canal instrumentation, i.e., PTU (Dentsply, Maillefer), PTG (Dentsply, Maillefer), and OC (Micro-Mega SA). After root canal preparation, samples were scanned again on micro-CT by the same scanning parameters. Surface area, canal volume, structure model index (SMI), percentage of uninstrumented area and transportation parameters were obtained for each sample before and after micro-CT analyse. Results: No significant differences between the PTG and PTU in terms of the total volume of removed dentin, surface area and percentage of uninstrumented areas were found. However, regarding to parameters above, OC showed a lower efficacy than PTG and PTU in coronal section. Regarding canal transportation, PTG and OC showed lower mean transportation values at all levels. Conclusion: This paper demonstrated the root canal shaping abilities of the PTU, PTG, and OC NiTi file systems. The PTG and OC systems were associated less canal transportation and a better ability to preserve dentinal walls than PTU. There was no significance different between all rotary file systems for SMI values however, PTU and PTG showed greater canal volume and surface area change than OC file systems in coronal section.

Long-term crop rotation and different tillage practices alter soil organic matter composition and degradation

Conservation agriculture such as conservation tillage and crop rotation can alter soil organic matter (OM) storage and cycling. However, little is known about how these methods alter the molecular-level soil OM composition and turnover. We collected soil samples (0−10 cm) from a 37-year experiment with contrasting diversity crop rotations under conventional tillage and conservation tillage. Soil organic carbon concentrations, molecular-level OM composition and microbial biomass and community structure were measured. Soil organic carbon concentrations were similar between the two tillage practices, likely due to the balance between carbon loss via degradation and carbon gains through incorporation of crop residues in soil. We observed significant changes in soil OM composition between conventional tillage and conservation tillage despite similar soil organic carbon concentrations. Under long-term conservation tillage practice, lower suberin-derived compounds and higher lignin-derived compounds were observed. The degradation of cutin-, lignin-derived compounds and soil OM were lower under conservation tillage among most rotations. The changes in lignin-derived compounds and degradation were microbially driven and likely controlled by substrate utilization. In contrast, the differences in suberin-derived compounds were likely associated with crop residue-induced OM inputs and/or the differential introduction of OM (e.g. root materials) to the upper layer of soil due to conventional tillage vs. conservation tillage managements. These findings reveal that microbial degradation, OM inputs from crop residues and management-induced OM distribution in soil layers may alter agricultural soil OM composition. Additionally, we found that the differences in some specific groups of compounds between conventional tillage and conservation tillage were lower in crop rotations with higher diversity, highlighting the benefits of the interaction between diverse rotations and conservation tillage.

Rated-M for mesocosm: allowing the multimodal analysis of mature root systems in 3D.

A plants’ water and nutrients are primarily absorbed through roots, which in a natural setting is highly dependent on the 3-dimensional configuration of the root system, collectively known as root system architecture (RSA). RSA is difficult to study due to a variety of factors, accordingly, an arsenal of methods have been developed to address the challenges of both growing root systems for imaging, and the imaging methods themselves, although there is no ‘best’ method as each has its own spectrum of trade-offs. Here, we describe several methods for plant growth or imaging. Then, we introduce the adaptation and integration of three complementary methods, root mesocosms, photogrammetry, and electrical resistance tomography (ERT). Mesocosms can allow for unconstrained root growth, excavation and preservation of 3-dimensional RSA, and modularity that facilitates the use of a variety of sensors. The recovered root system can be digitally reconstructed through photogrammetry, which is an inexpensive method requiring only an appropriate studio space and a digital camera. Lastly, we demonstrate how 3-dimensional water availability can be measured using ERT inside of root mesocosms.

Influence of embedding media on the accuracy of working length determination by means of apex locator: an ex vivo study

The aim of this research was to determine ex vivo the influence on accuracy of five different embedding media, for investigative and educational purposes, and one electronic apex locator. 110 human extracted mature roots of permanent single-rooted human teeth were used. The roots were embedded in alginate, stick sponge, 2% agar–agar and 6% and 12% gelatin. The actual working length to the physiological foramen was determined under a stereo-microscope (16 ×) and the electronic working lengths with the Elements Diagnostic Unit and a K-file ISO 10. The accuracy ranges of the accumulated measurements, when allowing a ± 0.5 mm tolerance, went from 98.2% (6% and 12% gelatin), 93.7% (alginate), 92.8% (2% agar–agar) to 91.7% (sponge). The exact measurements at the physiological foramen ranged from 80.0% (6% gelatin), 76.5% (2% agar–agar), 71.8% (12% gelatin), 68.2% (alginate) to 64.5% (sponge). Although relatively seldom (n = 24), measurements with deviations of more than ± 0.5 mm were also observed; thus, the accuracy of the working length determination results per se can be considered as clinically acceptable. The results of this research allow a recommendation of the investigated embedding media for electronic working length determination models for educational and research purposes in endodontics.

Growth regulation of Desmostachya bipinnata by organ-specific biomass, water relations, and ion allocation responses to improve salt resistance

Salt resistance strategies in Desmostachya bipinnata may be a function of coordinated organ specific responses for growth and developmental stages. It may provide clues to improve salt tolerance in conventional crops. The effects of NaCl concentrations (100, 200, 300 and 400 mM) on growth, water relations, organic compound (proline and sugar) accumulation, ion-flux and nutrient selectivity both in root [adventitious (RA) and mature (RM) root] and shoot [juvenile (LJ) and adult (LA) leaves] organs were investigated. Decreases in biomass were observed under saline treatments higher than 100 mM NaCl. The lower amount of biomass is considered to be a survival strategy under harsh conditions due to reallocation of energy in the plant. D. bipinnata regulated water flux (OP, osmotic potential, in the following order: LJ > LA > RM > RA) to allow water uptake from soil under hyperosmotic conditions. Na+ was largely accumulated in roots followed by LA and LJ. This ion excluding strategy is common in monocots to protect photosynthetic tissues. Salt-treated plants retained more Ca2+ and Mg2+ in roots than in shoots, while K+ allocation was not affected by salinity. This may be due to selective transport in the case of K+ and increasing use efficiency for Ca2+ and Mg2+. Whole plant responses generally are organ-specific based on salinity concentrations. Our findings suggest that different organs of D. bipinnata coordinated with each other for biomass partitioning, OP gradient, Na+ distribution, K+ selective transport, nutrient use efficiency, and sugar and proline allocation to achieve better yields in moderately saline areas for fodder/forage production.

Description of Mathematical Reasoning Ability on Vocational School Students in Square Root Material

The aim of this study is to describe the mathematical reasoning ability of students at TSM major (Motorcycle Engineering) Darul Karomah Singosari on square root material. This study applies descriptive qualitative approach, data was collected using test instrument that has been tested on three research subjects, and analyzed using qualitative descriptive technique. The results show that low- ability (Kemampuan Rendah) subject is the student with less mathematical reasoning ability because the subject has not been able to solve the five existing indicators of mathematical reasoning, middle- ability (Kemampuan Sedang) subject include student with moderate mathematical reasoning ability (although the subject has not fulfilled the five indicators of mathematical reasoning requirements), this can be seen from his effort to make a mathematical assumption even though the conjecture made was not correct, and high- ability (Kemampuan Tinggi) subject refers to the student with high mathematical reasoning ability because he can fulfill two of the five indicators of mathematical reasoning.

Decomposition of mangrove roots depends on the bulk density they grew in

Mangroves hold large organic carbon (C) stocks in their soils. These C stocks are mainly attributed to the high productivity and slow decomposition of the below-ground biomass. Rates of decomposition are strongly influenced by edaphic factors including soil bulk density (BD). Hence, this study assessed the influence of soil BD on mangrove root material and its effect on decomposition. To determine the effect of the BD in which the plants were grown on decomposition rates (i.e. mass loss), four mangrove species were grown in soils of six different BD. The root material was then placed in litterbags and incubated in mangrove forest soil for three months. Additionally, the root material was analysed for nitrogen (N) and phosphorus (P), as well as lignin and cellulose content. The soil BD experienced during root growth strongly affected mass loss, which decreased with increasing soil BD of the growth media. Decomposition also differed among species. Variation in mass loss was likely influenced by N levels, C:N ratios and lignin content of the root tissue. Variation in soil BD influences the C sequestration potential of mangroves, as BD affects the decomposability of mangrove root tissues.

Evaluation and Selection of Physical Soil and Water Conservation Techniques for Early Mature Sorghum Root Zone Moisture Retention at Harari, Erer Research Station

The experiment was conducted at Babille district, during 2018 and 2019 main cropping season at Erer Research Station. Erer research sub site is located latitude of 09° 10' 41.5" north and longitude of 042° 15' 27.3". The objectives of the experiments were to evaluate and select best methods of root zone moisture retention and soil conservation techniques suited for early mature sorghum. The treatments of experiments were, Control (no soil and water conservation measures), open ended tied ridge, level soil bund, contour furrow, closed ended tied ridge, Broad bed, Semi-circular and mulch respectively. The design of the experiment was RCBD with three replication. Genstats 18^th editions was used for data Analysis. Statistically there is highly significant variation among the treatments in terms of all parameters at 5% significance level across three planting years. First year, the highest plant height was obtained from the treatment with Broad bed followed by closed ended tied ridge. On other hand, the lowest plant height was recorded from the treatments with soil bund followed by control (no conservation structure). This is because of no more top fertile soil disturbance during first year. During the second and third planting year, the highest, plant height (132.86cm) and the longest panicle diameter (6.5) was recorded, from the treatments with open ended tied ridge, followed by soil bund and contour furrow respectively. That was because of soil and water conservation (swc) structures design and extra moisture harvested in those structure than rest treatments. During the first year of the experiment, the highest grain yield was obtained from the mulch followed by open ended tied ridge treatments. This is because of no and slight top soil disturbance during the construction stage. But, the third year, the maximum grain yield was recorded from contour furrow followed by open ended tied ridge (2130.9 and 2000kg ha^-1) respectively. The lowest yield was obtained from no conservation measures followed by mulching (1125 and 1232.1 kg ha^-1). The most probable reason for this variation can be due to the amount of the moisture harvested in the root zone of sorghum because of conservation structure. So contour furrow and open ended tied ridges are suitable swc measures for the area.

Comparative evaluation of removal of smear layer using newer rotary endodontic files: A scanning electron microscope study

Aim:The aim of this study is to evaluate the smear layer removal efficacy of newer rotary files using scanning electron microscopic analysis at the coronal, middle, and apical third of the root canal.Materials and Methods:In this study, sixty permanent human single-rooted teeth with complete, mature root apices without any anatomic variation having straight patent root canal extracted for periodontal cause were included. The samples were cleaned to remove debris, calculus and rinsed with sodium hypochlorite to remove organic tissue and then stored in distilled water. All the samples were decoronated with the help of carborundum disc at root length of 12 mm to obtain segments similar in length. After this, all the samples were randomly divided into four groups -Protaper universal, Protaper Gold, EDM, and Revo-S. After cleaning and shaping, samples in each group were finally flushed with normal saline. Samples were then dried. Grooves were made into the root in the buccal and lingual portion of the root using a diamond bur and the samples were split longitudinally into two halves using stainless steel chisel and mallet. Preparation for scanning electron microscope was done and phototmicrographs at ×3000 were taken.Result:Chi square test and Kruskal–Wallis test was used for statistical analysis. Result of this study showed that Revo –S was found to be best amongst the groups and EDM was the poorest.Conclusions:Based on the present study, Revo-S files showed the best smear layer removal in the coronal, middle, and apical 1/3rd of the root canal when compared to other groups. Within the parameters of the study, Revo –S was found to be best amongst the groups and EDM was the poorest. The quality of various files according to ranks of smear layer removal was found to be: Group IV > Group II > Group I > Group III.

Efficacy of different methods of calcium hydroxide removal on bond strength of calcium silicate-based sealer

AimTo evaluate the efficacy of different methods of calcium hydroxide removal [XP-endo finisher (XP-F) file, passive ultrasonic irrigation (PUI) and NaviTip FX (NTFX) irrigating needle] on push-out bond strength of Endoseal MTA calcium silicate-based sealer.Materials and methodsForty extracted human premolars with nearly straight mature roots were used in this study. Teeth were decoronated to obtain a standardized root length of nearly 15 ± 1 mm. Root canals were prepared using ProTaper Universal rotary system up to F4 (40/0.06). Thirty samples were filled with calcium hydroxide paste then after 7 days, it was removed using different methods: G1: XP-F, G2: PUI, G3: NTFX. The remaining ten teeth with no prior medication were used as a control group (G4). All samples were obturated using gutta-percha/Endoseal MTA then transversally sectioned into coronal, middle, apical sections. The obtained sections were subjected to push-out bond strength test using a universal testing machine.ResultsThe highest mean push-out bond strength values were recorded for the control group followed by XP-F file and PUI while the lowest value for NTFX groups. The coronal section recorded the highest mean push-out bond strength value while the lowest value for the apical section with a significant difference in all groups.ConclusionPush-out bond strength values for XP-F and PUI groups were higher than NTFX group.

Micro-CT evaluation of root canal preparation with rotary instrumentation on prototyped primary incisors.

This study aimed to evaluate the endodontic instrumentation outcomes with asymmetrical files compared to reciprocating and hand files (HFs) in 3D-printed prototypes of upper primary incisors using micro-computed tomography (micro-CT). For this purpose, 50 prototypes were randomly divided (n = 10) according to the instrumentation technique as follows: HFs, a reciprocating file (WaveOne® Gold [WOG]), and three asymmetrical movement files: XP-Endo® Shaper (XPS), XP-Endo® Finisher (XPF), and XP Clean (XPC). The specimens were scanned and, after registration of the baseline and instrumented volumes, changes in the root canal volume (RCV), debris accumulation, removed root material volume (RRMV), non-instrumented areas, and the presence of cracks/perforations were quantified. Data were analyzed by analysis of variance and Student's t-test, while the effect size was calculated for statistically significant outcomes. All groups showed an increase in RCV after instrumentation (p < 0.05), but this was higher with HFs (p < 0.05). Accumulated debris was higher for WOG and XPS (p < 0.05), but WOG exhibited more in the medium and apical third areas. HFs showed the highest RRMV (p < 0.05), especially at the apical third. The non-instrumented areas were lower for HFs and XPC than for the other systems (p < 0.05). Cracks were present in a few WOG (n = 2) and HF specimens (n = 3) and in this group, one of the cracked specimens and two others showed perforations. The asymmetric systems resulted in conservative dentin removal and fewer cracks/perforations as compared to HFs and a reciprocation file in prototyped primary upper incisors. XPC showed the best compromise between RRMV and non-instrumented areas with a low accumulation of debris.

Effect of isotretinoin on induced tooth movement in rats

This study was performed to evaluate the effect of isotretinoin on tooth displacement and tissues related to induced tooth movement (ITM) in rats. Wistar rats were randomly divided into 4 groups: vegetable oil (O; n=40), 7.5mg/kg isotretinoin (I; n=40), vegetable oil+ITM (OM; n=44), and 7.5mg/kg isotretinoin and ITM (IM; n=39). After the daily application of the solutions for 30days, an orthodontic appliance was installed to mesially displace the maxillary first right molar (30cN) of rats in the OM and IM groups. The animals were killed 2, 7, 14, or 21days after placement of the devices. The animals in the O and I groups did not undergo ITM but were killed simultaneously. The animals were examined for tooth displacement, the neoformation of mature collagen, bone and root resorption, the presence of hyalinized areas, and trabecular bone modeling by microcomputed tomography. There was no difference in tooth displacement, the number of osteoclasts, the presence of hyalinized areas, or trabecular bone among the O, I, OM, and IM groups across the periods tested (P>0.05). A lower percentage of mature collagen was found in the IM group than in the OM group on day 7 (P<0.05). A lower frequency of root resorption was found in the IM group than in the OM group on days 2 and 21 (P<0.05). Isotretinoin at 7.5mg/kg decreased root resorption in rats subjected to ITM.

First report of root mat disease in a hydroponic tomato production system caused by rhizogenic Agrobacterium biovar 1 in South Korea.

In June 2019, root mat disease was observed in hydroponically cultivated tomatoes in Jinju, South Korea, which occurred in at least 30% of the plants in the greenhouse. To isolate the causal bacteria, 10 g of infested tomato root mat sample was ground with 50 mL of sterile water. A 100-µL aliquot of the homogenate was serially diluted and spread on Mannitol-Glutamate (MG) medium amended with 0.1% yeast extract (MGY) and incubated at 28°C for 48 hours. Fifteen dominant colonies that formed on the MGY medium were purified and subjected to diagnostic polymerase chain reaction (PCR) based on the virD2-ipt gene loci. Because Ti-plasmid harbors both virD2 and ipt genes, Ri-plasmid-borne Agrobacterium species with only virD2 can be differentiated using virD2-ipt PCR. To amplify virD2, the primers 5'-ATG CCC GAT CGA GCT CAA GT-3' and 5'-TCG TCT GGC TGA CTT TCG TCA TAA-3' were used; for ipt amplification, the primers 5'-GAT CG(G/C) GTC CAA TG(C/T) TGT-3' and 5'-GAT ATC CAT CGA TC(T/C) CTT-3' were used. Amplification involved an initial 94°C for 1 min and then 40 cycles at 94°C, 50°C, and 72°C for 1 min at each temperature, with a final 5-min extension at 72°C. For three strains (GNIY2, J10, and J11), virD2 PCR products, but no ipt PCR products, were identified, indicating that three strains are Ri-plasmid-borne Agrobacterium species. A pathogenicity test was performed on 2-week-old tomato plants. Bacteria isolates (GNIY2, J10, and J11) cultured overnight in LB were made into a bacterial suspension (107 cfu/mL) in 50 mM phosphate buffer. Five tomato seedling roots were cut with sterilized scissors and soaked in each bacterial suspension for 1 hour. Phosphate buffer was used as a negative control. The inoculated tomato seedlings were transplanted in new pots and placed in a greenhouse with 25°C day and 20°C night temperature set points in natural light. After 9 weeks, all inoculated tomato plants produced overgrown roots, while the negative control plants had no symptoms. To satisfy Koch's postulates, re-isolation was performed from the diseased tomato and was the re-isolated bacteria were subject to partial 16S rDNA sequencing. Biovar tests performed as previously described revealed that all three isolates were biovar 1. A representative strain (GNIY2) was deposited in the Korean Agricultural Culture Collection (KACC 21759). To confirm the identity, four housekeeping genes of KACC 21759 were sequenced (16S rRNA, trpE, rpoB, and recA) and deposited in GenBank (accession nos. MT071560, MT444428, MT444429, and MT444430). Multilocus sequence analysis performed as previously described showed that the KACC 21759 strain was grouped in Agrobacterium genomospecies 4. This is the first report on mat root disease caused by Agrobacterium biovar 1 in hydroponic tomatoes in South Korea.

The Effectiveness of Different Priming Treatments on Seeds of Eggplant (Solanum melongena L.) Varieties

Bu araştırmada, farklı patlıcan çeşitlerine ait tohumların hidropriming (HP), ozmopriming (OP) ve termopriming (TP) uygulamalarının tohum çimlenme kalitesi üzerine etkileri araştırılmıştır. Manisa kır çizgili (MKÇ), Kemer 27 (K) ve Aydın Siyahı (AS) çeşitlerinin farklı olgunluk seviyelerinde hasat edilen (çiçeklenmeden sonraki 50, 55, 60 ve 75 gün sonra) patlıcan tohumları 35 0C’de 4 ve 8 saat (1:2/karanlık-aydınlık koşulda) süre boyunca, %10’luk PEG-6000 ile OP, 35 0C’de 4 ve 8 saat (1:2/karanlık-aydınlık koşulda) sürede saf su ile HP ve 50°C’de 2 farklı sürede (15 ve 30 dakika) su banyosunda TP işlemlerine tabi tutulmuştur. Tüm çeşitlerde kontrol ve uygulama gruplarında tohum nem miktarları belirlenmiştir. Uygulamalar sonunda tohumlar yüzey kuruluğu sağlanıncaya kadar bekletilerek ardından petri çimlendirme testine tabi tutulmuştur. Denemeler sonucunda, patlıcan tohumlarının OP, HP ve TP uygulamaları ile canlılık (ortalama çimlenme oranı ve süresi, fide kök-sürgün uzunlukları) bazında değerlendirilmiştir. İncelenen parametreler değerlendirildiğinde, priming olumlu etkisi tohumların olgunluğu ile ilişkili olduğu görülmüştür. Bağımlı değişkenler olan normal ve toplam çimlenme parametreleri; priming, çeşit, olgunluk ve bunların interaksiyonu ile istatistiksel olarak anlamlı bulunmuştur (p

Effects of withania somnifera root extracts on serotonin secretion in suiz albino mice

Pain is a common manifestation of presence of disease or physical injury in humans. High levels of serotonin in the plasma has been associated with pain. This study aimed at determining the effect of Withania somnifera root extracts on serotonin levels in Suiz albino mice. W. somnifera root samples were collected and ground to small pieces. Ethanol, n-butanol, xylene and methanol were used to extract metabolites from the ground root materials using hot extraction technique. The mice were injected with the extracts separately and serotonin levels determined over a period of 12 h at 2 h intervals. Serotonin concentration was determined using Beer Lamberts method. Light absorbance by the extracts varied significantly (F = 50.2011 P = 1.25x10-07. The concentrations of serotonin from mice injected with the metabolites from the selected solvents varied significantly (F = 23.3269 P = 0.0000469. In addition, there was no significant difference in light absorbance values obtained by Panadol, methanol and ethanol extracts. (F = 3.7178 P = 0.089). Extracts from W. somnifera have the ability to reduce serotonin levels in plasma. Further studies on the mechanisms involved in reducing serotonin levels by W. somnifera in blood need to be carried out. There is need for mass production of metabolites from W. somnifera. Keywords: Withania somnifera, Root extracts, Serotonin, Suiz albino mice

Smart Root Search (SRS): A Novel Nature-Inspired Search Algorithm

In this paper, a novel heuristic search algorithm called Smart Root Search (SRS) is proposed. SRS employs intelligent foraging behavior of immature, mature and hair roots of plants to explore and exploit the problem search space simultaneously. SRS divides the search space into several subspaces. It thereupon utilizes the branching and drought operations to focus on richer areas of promising subspaces while extraneous ones are not thoroughly ignored. To achieve this, the smart reactions of the SRS model are designed to act based on analyzing the heterogeneous conditions of various sections of different search spaces. In order to evaluate the performance of the SRS, it was tested on a set of known unimodal and multimodal test functions. The results were then compared with those obtained using genetic algorithms, particle swarm optimization, differential evolution and imperialist competitive algorithms and then analyzed statistically. The results demonstrated that the SRS outperformed comparative algorithms for 92% and 82% of the investigated unimodal and multimodal test functions, respectively. Therefore, the SRS is a promising nature-inspired optimization algorithm.

Regenerative Pulpotomy as a Novel Technique for Treatment of Permanent Mature Molars Diagnosed with Irreversible Pulpitis Using Platelet-Rich Fibrin: A Case Series Study

INTRODUCTION: Management of teeth with clinical signs/symptoms suggestive of irreversible pulpitis was conventionally invasive, but the emerging evidences suggested successful treatment outcome using the less invasive vital pulp procedures such as coronal pulpotomy, leading to preservation of the remaining pulp in a vital and functioning state. OBJECTIVES: Evaluation of the clinical/radiographic success of novel regenerative coronal pulpotomy technique using Platelet-Rich Fibrin(PRF) and Biodentine. METHODOLOGY: Three irreversibly inflamed permanent molars with mature roots in three patients were treated by regenerative pulpotomy technique. Access openings were done, and coronal pulps were removed to the level of root canal orifices. Hemostasis in all teeth were done by the compression with cotton pellets moistened with 2.5 % sodium hypochlorite(NaOCl). PRF membranes were prepared by drawing 10 ml of patients’ own blood, then they were immediately centrifuged using a table top centrifuge at 400 gforce for 12mins. The PRF membranes were placed over the remaining radicular pulps followed by Biodentine preparation and placement over the PRF. The cavities were then immediately restored. Patients were scheduled for clinical/radiographic evaluations after three-, six- and 12-months. RESULTS: Throughout the follow-up periods, the three teeth demonstrated clinical/radiographic success with complete resolution of clinical signs/symptoms. CONCLUSIONS: All presented cases showed a favorable clinical/radiographic outcomes of “Regenerative pulpotomy” procedure in treating irreversibly inflamed permanent molars using PRF and Biodentine combination.