1. The physiological reactions of the three types of Indian nodule bacteria (C. indicus, D. biflorus andPs. tetragonolobus) have been studied and ascertained, as is indicated in the tables. 2. In the isolation of the nodule organism fromCajanus indicus roots we found with certain characters of constancy a contaminant or rather a concomitant, which has not the characteristics ofB. radiobacter, the only organism likely to be met with. 3. TheCajanus indicus root nodule organism is placed by various authors Lohnis and Leonard, in the Cowpea group, the members of which have got an alkaline reaction on milk medium and also on nearly all sugars. The organism ofC. indicus isolated by us differs in this respect from the members of the Cowpea group in having acid reaction on litmus milk, and mixed reactions on sugars, acidic and alkaline. 4. The use of gelatin stabs, commonly followed in the study of gelatin reactions, may account for much of the irregularity of reaction and disagreement observed in the conclusions of different authors. Our procedure of the gelatin slants gives clear results, sometimes in half the time, and great advantage will be derived from its general adoption. 5. Garman and Didlake’s method proved successful as regards the culturing of host plants, but not as regards the nodule formation. Pending the study of a modification of the same, we devised an easy and reliable method of pot cultures which can be depended upon to give clear results in our exacting conditions of temperature and moisture.
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