The rapid advancements in Artificial Intelligence (AI) have significantly shifted the landscape of work, altering the roles of experts and knowledge workers across various domains (Humala et al. 2023). This paper explores the pivotal role of key competencies – self-efficacy, job crafting, and proactivity – in navigating the technological transformation brought about by AI in work settings. The three key competencies were previously identified as the main building blocks of the entrepreneurial spirit that is necessary for the successful use of AI in knowledge work and organizations (Humala & Lahtinen 2023, Ruohonen & Humala 2022). The research paper draws insights from a quantitative nation-wide survey that was carried out among knowledge workers in Finland. The survey was conducted during February-March 2024, with the insights gained from 474 informants. The research was funded by The Finnish Work Environment Fund and conducted by Haaga-Helia University of Applied Sciences based in Helsinki. Results show that while the integration of AI in knowledge work environments generally supports self-efficacy, job crafting, and proactivity, the effects are varied, showing both enhancement and, in some cases, diminishment. The findings indicate that AI’s positive influence is most pronounced among those engaged in high levels of knowledge-based tasks and who maintain a positive outlook toward new technologies. This research also highlights some disparity in experiences between different groups, particularly between younger and older knowledge workers with early- and late-careers as well as those in different work positions, pointing to the need for tailored organizational support to ensure equitable benefits from AI technologies. The research reveals that AI can significantly foster proactivity in the workplace, particularly when employees are equipped with sufficient information and resources. Ultimately, this exploration aims to provide insights into developing AI-supported business models that not only leverage the human entrepreneurial spirit but also foster an inclusive environment that enhances organizational competitiveness and innovation.