In the past two decades, the corrosion failures of rock reinforcement bolts in underground coal mines have been increasingly reported. Preliminary studies have shown that these failures were predominantly related to pitting and stress corrosion cracking. The analyses in affected mines indicated microbially induced corrosion (MIC) as one of the primary corrosion causes. As such, there is an urgent demand from industries to develop methods to mitigate MIC-associated failures of rock bolts in underground coal mines. This study examined epoxy coating to determine its effectiveness in preventing biofilm formation on steel surfaces and, in turn, averting MIC. The corrosion-causing bacteria were isolated and enriched from groundwater samples collected from the affected mine sites. Coated and uncoated rock bolt samples were prepared from the bolts and incubated in media in the absence and presence of the corrosion-causing bacteria. Fluorescence microscopy imaging found no evidence of bacterial biomass growth on the surface of the epoxy-coated steel surfaces after 30 days, while the non-coated surfaces were colonised by biomass. The observations suggest the potential of epoxy coating for bolt MIC prevention. Future studies to assess the applicability of epoxy coating in the underground mine environment are recommended.