Aggressive Risk Research Articles

Patient-Derived Meningioma Organoids: A Reliable Model for Studying Human Tumor Pathophysiology

Introduction: Meningiomas are the most common primary central nervous system tumors, constituting 39.7% of intracranial tumors. Although generally benign, some exhibit aggressive behavior and risk of recurrence, necessitating adjuvant therapy and repeat surgical interventions. Molecular studies have identified tumor-driving mutations, leading to targeted therapies and clinical trials. However, translating preclinical findings into clinical success is often hindered by limitations in current meningioma tumor models. This study aims to develop and validate a standardized protocol for establishing patient-derived meningioma organoids (MEN-Os) that faithfully replicate human disease. Methods: MEN-Os were successfully established from 15 meningioma samples (11 grade 1, 4 grade 2) from neurosurgical resections using an optimized culture protocol. Histological and immunohistochemical analyses were used to assess the resemblance of MEN-Os to original tumor tissues. RNA sequencing compared transcriptional signatures between MEN-Os and corresponding patient-resected tissues. Results: MEN-Os were successfully established from patient-resected samples and maintained in culture for up to four weeks, showing stable growth and structural integrity. Histopathological analysis revealed that MEN-Os preserved key architectural features, including cellular organization, nuclear morphology, and proliferation rates. Immunohistochemical staining for meningioma-specific markers, such as the progesterone receptor, confirmed similar expression patterns to parental tumors. Transcriptomic profiling demonstrated that MEN-Os retained the transcriptional signatures of original tissues, including genes associated with meningioma pathology (NF2, CDKN2A, TP53). Differential expression and deconvolution analyses showed that MEN-Os contained diverse cell populations, including tumor and stromal cells, while preserving the immune microenvironment, as validated by histopathological and transcriptomic profiling. Conclusion: We established a robust, reproducible protocol for generating MEN-Os, which faithfully replicates the histopathological, molecular, and cellular characteristics of original tumors. MEN-Os provide a valuable model for studying meningioma biology and evaluating therapeutic strategies.

Association Between the Aromatase rs2414096 Polymorphism and Prostate Cancer

Abstract Objectives The purpose of this study was to investigate association between the aromatase (CYP19A1) rs2414096 polymorphism and the risk of prostate cancer development, progression and aggressiveness in the Slovak population. Background Aromatase catalyses the final reaction of androgens aromatization to estrogens, irreversible conversion of androstenedione to estrone and testosterone to estradiol. Methods The study population consisted of 721 prostate cancer patients and 660 men in the control group. Genotyping was performed via restriction fragment length polymorphism analysis. The expression levels of CYP19A1 in prostate cancer tissues were compared with those in benign prostatic hyperplasia tissues. Results The CYP19A1 rs2414096 AA genotype and A allele were significantly associated with an increased risk of prostate cancer development. The significant association between this genotype and PSA level ≥ 10 ng/ml, Gleason score ≥ 7 and with the presence of metastases was observed. Significantly decreased relative expression of CYP19A1 was detected in prostate cancer tissues. Patients with the AA genotype presented higher relative CYP19A1 expression than did those with the GG genotype. Significantly lower circulating testosterone levels and higher serum PSA levels were detected in patients with the AA genotype. Conclusion The CYP19A1 rs2414096 polymorphism was significantly associated with prostate cancer development and aggressiveness in Slovak patients.

The role of changes in peer victimization from elementary to high school and autonomic reactivity on adolescent reactive aggression.

The goals of the present study were to investigate links between changes in peer victimization from elementary to high school and adolescent reactive aggression (Goal 1), whether heightened autonomic nervous system (ANS) reactivity to social and nonsocial stress increases risk for adolescent reactive aggression (Goal 2), and whether increased ANS reactivity strengthens the association between changes in victimization and adolescent reactive aggression (Goal 3). Participants included 145 adolescents (Mage = 16; 54% female; 76% European American, 13% African American, 11% Latino American, 7% Asian American, 5% of mixed race or ethnicity; 60% with family incomes of $100,000 or greater). We collected self-report data in elementary (Time 1 [T1]); (Time 2 [T2]); middle (Time 3 [T3]); and high school (Time 4 [T4]) to assess victimization. At T4, we measured self-reported reactive and proactive aggression, and ANS reactivity (preejection period [PEP], respiratory sinus arrhythmia [RSA]) to peer rejection and nonsocial frustration. More positive victimization slope over time (meaning both less decreasing slopes and increasing slopes) predicted greater adolescent reactive, but not proactive aggression (Goal 1). Greater RSA augmentation to peer rejection and more PEP reactivity to nonsocial frustration predicted more reactive, but not proactive aggression (Goal 2). The link between victimization slope and reactive aggression emerged only for adolescents exhibiting RSA augmentation to peer rejection (marginal; Goal 3). (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Evaluating the relationship between marijuana use, aggressive behaviors, and victimization: an epidemiological study in colombian adolescents.

This study aimed to examine the relationship between marijuana use and aggression and victimization among Colombian adolescents. We aimed to clarify marijuana's distinct role by comparing different categories of drug use and by considering the order of drug initiation. We conducted a secondary analysis of nationally representative cross-sectional data collected from Colombian adolescents in 2016. The original sample included 80,018 students in Grades 7 to 11. Participants were categorized into marijuana-use groups- EXCLUSIVE (marijuana only), INITIAL (marijuana use before other drugs), and SUBSEQUENT (marijuana use following other drugs)- and non-marijuana-use groups- NON-DRUG (no use), ONE-DRUG (one other drug only), and MULTIPLE-DRUG (two or more other drugs).Aggressive behaviors (individual aggression, group aggression, harassment) and victimization were assessed based on self-reported involvement in the past 12months. Logistic regression models examined associations between marijuana use patterns and these outcomes, controlling for sex, age, parental education, and grade repetition. For the SUBSEQUENT group, the total number of other drugs used was also controlled. Adolescents with no drug use had the lowest rates ofall aggressive behaviors and victimization. As drug use increased, so did the prevalence of these outcomes, with MULTIPLE-DRUG users exhibiting the highest levels. Compared to NON-DRUG adolescents, each marijuana-use group (EXCLUSIVE, INITIAL, SUBSEQUENT) showed increased odds of some forms of aggression and victimization. For example, EXCLUSIVE users had higher odds of aggression compared to NON-DRUG users. However, the magnitude of these associations differed when comparing marijuana-use groups against each other andagainst ONE-DRUG and MULTIPLE-DRUG groups. INITIAL and SUBSEQUENT users often demonstrated greater odds of aggression than EXCLUSIVE users, suggesting that polydrug involvement and the sequence of drug initiation matter. These findings underscore the importance of moving beyond binary classifications of marijuana use when examining aggression and victimization among adolescents. Marijuana use is associated with an increased risk of aggression and victimization, but other substance use patterns and the temporal order of drug initiation influence this relationship. Policymakers, educators, and clinicians should consider these when designing preventive interventions. Future research should employ longitudinal designs and incorporate additional contextual variables to further clarify the mechanisms linking marijuana use to aggression and victimization.

Abstract B030: Novel strategy to improve response of high risk HPV+ HNSCC to radiation therapy

Abstract Background: Improving understanding of head and neck squamous cell carcinoma (HNSCC) has led to the discovery of novel molecular markers to stratify risk and guide treatment decisions. Recently, we have identified two subtypes of HPV+ OPSCC – one with good prognosis and one with poor prognosis. The subtypes are distinct in many ways – somatic genes mutated, HPV viral oncogenes expression, the physical state of the viral genome (integrated vs episomal), as well as global mutation and methylation profiles. Intriguingly, all differences converge on intrinsic tumor NF-κB activity with constitutively active NF-κB (usually arising from genetic defects in NF-κB regulators, including TRAF3 and CYLD) driving significantly elevated radiation sensitivity and improved patient survival. NF-κB-based groups define an important clinically actionable prognostic biomarker in HPV+ HNSCC: one important goal is to de-escalate therapy for patients with molecularly low-risk tumors to improve quality of life amongst survivors, while another goal is to improve the cure for of patients with a high risk of treatment failure. Material and methods: We utilized HPV+ head and neck cancer cells, HPV+ HNSCC xenografted mouse model, patient-derived xenografts (PDX), as well as our novel fully immunocompetent mouse model of high risk HPV+ HNSCC; we applied targeted fractionated radiation. To activate NF-κB, we used two clinically applicable drugs with different mechanisms of action: Toll-like receptor 5 (TLR5) agonist and mitochondria-derived activator of caspases (SMAC) mimetic. Custom NF-κB NanoString panel contains our recently developed NF-κB gene signature along with HPV genes and housekeeping genes that are used to normalize the gene expression data. Results: Both drugs activated NF-κB in HPV+ HNSCC and significantly improved response of HPV+ head and neck cancer cells and tumors to radiation. Interestingly, TLR5 agonist alone had a strong tumor suppressing effect in low NF-κB HPV+ HNSCC; this effect was significantly better than 32 Gy focused radiation given in fractions of 4 Gy. Custom NF-κB NanoString panel is robustly prognostic with NF-κB active tumors having improved outcomes after chemoradiation. Conclusions: Because of the average relatively favorable prognosis of HPV+ HNSCC, urgent need for improved therapy for patients with aggressive high risk tumors is greatly undervalued. Here, we present a clinical assay to risk stratify patients with HPV+ HNSCC and begin to explore pharmacological activation of NF-κB as a strategy to sensitize high risk HPV+ HNSCC to radiation therapy. TLR5 agonist is well known for the remarkable normal tissue protective effects against radiation and other damaging conditions; the dual effect of entolimod – sensitization of HPV+ HNSCC to radiation, while simultaneously protecting normal tissues from severe side effects - may be useful for patients with high-risk HPV+ head and neck cancer in combination with radiotherapy. Citation Format: Sri Vemulamanda, Natalia Issaeva1, Aditi Kothari, Travis Schrank, Wendell G. Yarbrough. Novel strategy to improve response of high risk HPV+ HNSCC to radiation therapy. [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr B030.

Early Detection of the Pathogenetic Variants of Homologous Recombination Repair Genes in Prostate Cancer: Critical Analysis and Experimental Design

It has been shown that the pathogenic variants (PVs) of the DNA Damage Response (DDR) genes, whether of a germinal or somatic nature, represent a predictive biomarker of high sensitivity to treatment with inhibitors of the enzyme poly-ADP-ribose polymerase (PARP) in patients with hormone-resistant metastatic prostate cancer (HRPCa). Moreover, the detection of PVs of the Homologous Recombination Repair (HRR) genes in PCa patients can help to define the patient’s prognosis and the choice of the therapeutic procedure. Among men with metastatic PCa, the frequency of PVs in HRR genes ranges from 11% to 33%, which is a significantly higher rate compared to non-metastatic PCa, where the incidence is between 5% and 10%. Next-Generation Sequencing (NGS) results were more commonly obtained from newly acquired somatic samples compared to archived samples (prostate biopsy or prostatectomy). We developed an experimental multidisciplinary prospective study in patients with a new diagnosis of high-risk PCa at biopsy. The aim was to evaluate the presence of PVs of different HRR genes in patients with the first diagnosis of PCa in relation to a metastatic or non-metastatic stage, tumor aggressiveness, and early risk of progression. Among 43 initial tumor samples from 22 patients, 25 samples from 12 patients were selected for library preparation based on their DNA concentration and quality. After the NGS, 14 different DNA variants were prioritized. Oncogenetic and likely oncogenetic variants were found in the ATM, BRCA1, PTEN, KMT2D, and CDH1 genes. Moreover, variants of uncertain significance were found in ATM, DDR2, FANCA, FOXA1, PLCB4, PTCH1, and RB1.

Metabolic pathway modulation by olanzapine: Multitarget approach for treating violent aggression in patients with schizophrenia.

The use of network pharmacology and blood metabolomics to study the pathogenesis of violent aggression in patients with schizophrenia and the related drug mechanisms of action provides new directions for reducing the risk of violent aggression and optimizing treatment plans. To explore the metabolic regulatory mechanism of olanzapine in treating patients with schizophrenia with a moderate to high risk of violent aggression. Metabolomic technology was used to screen differentially abundant metabolites in patients with schizophrenia with a moderate to high risk of violent aggression before and after olanzapine treatment, and the related metabolic pathways were identified. Network pharmacology was used to establish protein-protein interaction networks of the core targets of olanzapine. Gene Ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were subsequently performed. Compared with the healthy group, the patients with schizophrenia group presented significant changes in the levels of 24 metabolites related to the disruption of 9 metabolic pathways, among which the key pathways were the alanine, aspartate and glutamate metabolism and arginine biosynthesis pathways. After treatment with olanzapine, the levels of 10 differentially abundant metabolites were significantly reversed in patients with schizophrenia. Olanzapine effectively regulated six metabolic pathways, among which the key pathways were alanine, aspartate and glutamate metabolism and arginine biosynthesis pathways. Ten core targets of olanzapine were involved in several key pathways. The metabolic pathways of alanine, aspartate, and glutamate metabolism and arginine biosynthesis are the key pathways involved in olanzapine treatment for aggressive schizophrenia.

Can Peer Acceptance and Perceptual Accuracy Impact the Effectiveness of Two Formats of a Preventative Intervention on Functional Subtypes of Aggression in Youth?

Coping Power (CP) is an empirically supported school-based intervention for children at risk for aggression. A child's social status with peers and the extent to which they accurately perceive it are important aspects of preadolescent social development that may influence how intervention format affects disruptive behavior outcomes. Further, reactive (RA) and proactive (PA) functional subtypes of aggression have differential relations with peer acceptance. This study is the first to test whether the effects of group (GCP) and individual (ICP) format of CP on RA and PA differed based on children's actual social status (aim 1) and whether they over- or underestimated their acceptance relative to their actual social status (perceptual accuracy; aim 2). This study involved secondary data analyses using a large-scale randomized controlled trial that assigned 360 children ages 9 to 11 (M = 9.74, SD = .62), predominantly male (n = 234, 65%), and Black (n = 273, 75.8%), with elevated levels of aggression to either ICP or GCP condition. Polynomial regression analyses and three-dimensional response surface plots tested and probed significant (p < .05) interactions between either actual acceptance or perceptual accuracy and intervention format on postintervention reactive and proactive aggression. Actual acceptance moderated the effects of GCP on RA, such that those with higher acceptance showed smaller reductions in RA from either preintervention or postintervention to follow-up. Perceptual accuracy also moderated the effects of ICP on PA, with those underestimating their acceptance showing smaller decreases in PA from postintervention to follow-up. These findings provide valuable insights into how children's actual peer acceptance and perceptual accuracy influence CP outcomes for different functional subtypes of aggression based on intervention format, raising important questions about potential mechanisms.

Impact of Cognitive Behavior Coping Skills on Relapse Prevention in Individuals with Methamphetamine Use Disorder

Methamphetamine use has spread throughout the world, needing a thorough assessment of the efficacy of different therapeutic approaches. The effectiveness of cognitive behavior coping skills in preventing relapses in methamphetamine addicts was assessed in this study. People with methamphetamine dependence at addiction treatment and rehabilitation facilities in Pakistan participated in a randomized controlled trial. The study hypotheses were evaluated using four questionnaires. A total of 60 participants were split into two groups: 30 in the experimental group and 30 in the control group. Relapse rates were significantly lower in the treatment group. In order to help people manage methamphetamine cravings as a chronic stressor that threatens recovery from substance use disorder, these findings highlight the significance of cognitive-behavioral coping skills in promoting positive emotions. which could be crucial in assisting people in controlling methamphetamine cravings, a persistent stressor that jeopardizes substance use disorder recovery. Significant changes were found to originate within subjects, according to the results of the Repeated Measure ANOVA, which was conducted using SPSS 26. The results indicate significant group differences on the variables of aggression, methamphetamine craving, coping skills, and stimulant relapse risk. The results of this study demonstrated that cognitive behavior coping skills interventions can lower aggression and craving levels as well as the likelihood of relapse in people with methamphetamine use disorder. Cognitive behavior interventions are also recommended as a treatment for improving personnel factors like self-efficacy and quality of life coping. According to the study's findings, providing cognitive behavior coping skills to an experimental group in an inpatient setting was both feasible and successful when compared to standard inpatient treatment. Cognitive behavior coping skills (CBCS) were found to be effective in preventing relapses in methamphetamine-dependent individuals. This study will further our understanding of CBCS as an evidence-based intervention for substance use disorders.

Clinicopathologic stratification demonstrates survival differences between endometrial carcinomas with mismatch repair deficiency and no specific molecular profile: a cohort study.

Endometrial carcinomas with mismatch repair deficiency (MMRd) and no specific molecular profile (NSMP) are considered to have intermediate prognoses. However, potential prognostic differences between these molecular subgroups remain unclear due to the lack of standardized control for clinicopathologic factors. This study aims to evaluate outcomes of MMRd and NSMP endometrial carcinomas across guideline-based clinicopathologic risk groups. This study analyzed patients treated at a single tertiary center. Immunohistochemistry and polymerase-ϵ sequencing were performed for molecular classification. MLH1-deficient tumors underwent methylation-specific multiplex ligation-dependent probe amplification. Carcinomas were classified into clinicopathologic risk groups according to European guidelines. The analysis included 420 MMRd and 399 NSMP carcinomas. Among MMRd cases, 224 were subcategorized as MLH1-methylated or MLH1-non-methylated. Median follow-up was 71 months (range; 1-136). Survival differences were most notable in clinicopathologic medium-risk carcinomas, with the MMRd subgroup exhibiting poorer progression-free, disease-specific, and overall survival compared to NSMP. Adjusting for age and adjuvant therapy, MMRd still showed an association with progression-free survival. Both MLH1-methylated (n = 154) and MLH1-non-methylated tumors (n = 70) were associated with more aggressive clinicopathologic risk groups compared to NSMP, but only methylated tumors showed poorer outcomes. The distinct outcomes for MMRd and NSMP in the clinicopathologic medium-risk group suggest that uterine risk factors may worsen the prognosis for MMRd endometrial carcinomas. Advanced stage may be the primary factor contributing to poor outcomes in high-risk-advanced metastatic carcinomas. Clinicopathologic factors may particularly worsen the prognosis of MLH1-methylated carcinomas.

Impact of diabetes mellitus on the progression of coronary artery disease: An observational study

Background: Diabetes mellitus (DM) accelerates the progression of coronary artery disease (CAD), increasing the risk of cardiovascular complications and mortality. Aims and Objectives: The aims of this study were to evaluate the impact of DM on the progression of CAD over a 2-year period and identify key risk factors and biomarkers associated with adverse cardiovascular outcomes in diabetic patients. Materials and Methods: This observational study included 100 participants, divided into two groups: 50 patients with DM and 50 non-diabetic controls. Demographic and clinical characteristics, including age, gender, body mass index (BMI), and baseline CAD characteristics, were recorded. Participants were followed for 2 years to assess the progression of CAD and new cardiovascular events. Risk factors such as hemoglobin A1c (HbA1c) levels, low-density lipoprotein (LDL) cholesterol, and C-reactive protein (CRP) were also measured. Statistical analyses, including multivariate logistic regression, were performed to identify independent predictors of CAD progression. Results: The diabetic group had a significantly higher BMI (29.1±4.6 kg/m1 vs. 26.5±3.9 kg/m1r P=0.01) and prevalence of CAD (80% vs. 60%, P=0.03). CAD progression was observed in 56% of diabetic patients compared to 30% of controls (P=0.01). New cardiovascular events occurred in 24% of diabetics versus 12% of controls (P=0.08). Diabetic patients had higher HbA1c (8.2%±1.1 vs. 5.6%±0.7, P&lt;0.001), LDL cholesterol (130.4±25.6 mg/dL vs.115.8±22.3 mg/dL, P=0.04), and CRP levels (3.2±1.4 mg/L vs. 1.8±1.0 mg/L, P=0.001). DM was an independent predictor of CAD progression (OR=2.9, 95% CI=1.4–5.8,P=0.003). Conclusion: DM significantly impacts CAD progression, emphasizing the need for aggressive risk factor management in diabetic patients.

Unveiling The Myth of High Recurrence Rate of Extracranial Arteriovenous Malformations of The Head and Neck: Systematic Review of Case Reports and Case Series

Background Arteriovenous malformations (AVMs) in the head and neck pose a challenge in their management due to their local aggressiveness and high recurrence risk. This study aimed to analyze literature on head and neck AVM recurrence post-treatment and identify the most effective strategy with a lower recurrence rate. Objectives To analyse existing literature on the recurrence of head and neck AVMs following treatment. Our goal was to identify the most effective treatment option with a lower recurrence rate. Methods We conducted a thorough literature search using PubMed, ScienceDirect, and Scopus, from year 2000 to the present. Our analysis focused on key endpoints, specifically the recurrence rates of head and neck AVMs following various treatment approaches. Results Out of the initial pool of 108 screened articles, a total of 83 patients were deemed suitable for inclusion in the literature review. The reviewed articles demonstrated that appropriate diagnostic tests were documented in 95% of the included studies. Among the patients, 37.3% had previously undergone interventions and were currently dealing with regrowth masses. Notably, 55.4% of patients underwent a combined approach involving both endovascular and surgical methods, while 25.3% opted for a surgical-only approach, and only 19.3% pursued an endovascular-only approach. The studies showed a promising curing rate of AVMs, with a success rate of 94%, albeit with a complication rate of 32.5%. The average follow-up duration for all patients was 26 months, with a standard deviation of 20.5 months. Out of the 83 patients, 5 experienced recurrence, with single-modality approach. Interestingly, no patients who received a multi-modality of treatments experienced recurrence or regrowth of the AVM mass within the follow up period. Conclusion The multi-modality approach outperformed single-modality treatments in preventing AVM recurrence. These findings highlight the importance of a comprehensive and multidisciplinary approach in the management of these complex vascular anomalies. PROSPERO: CRD42023490871 registered on 17/12/2023

Chronic Traumatic Encephalopathy, Family History of Mental Illness, and Aggression in Brain Donors With Repetitive Head Impact Exposure.

Chronic traumatic encephalopathy (CTE) is a neurodegenerative tauopathy associated with exposure to repetitive head impacts, including from contact sports and military service. Although CTE case reports have commonly described aggression during midlife, recent studies failed to show associations between CTE tau burden and aggression. First-degree family history of mental illness (1°FHMI) is a well-established risk factor of aggression. We tested the hypothesis that CTE pathology moderates the association between 1°FHMI and aggression, providing an explanation for the lack of association previously observed. This was a retrospective examination of consecutive, deceased, male brain donors with repetitive head impact exposure from the Understanding Neurologic Injury and Traumatic Encephalopathy Study at Boston University from 2014 to 2021. Neuropathologists diagnosed CTE using established National Institute of Neurological Disorders and Stroke criteria. Informants were administered the Brown-Goodwin Assessment for Lifetime History of Aggression (BGLHA) and were queried regarding 1°FHMI. Exploratory factor analysis evaluated BGLHA factor structure. Stratified by CTE status, linear regression analyses examined relationships between 1°FHMI and standardized adult BGLHA scores and factor scores. Models were adjusted for race, age at death, education, years of contact sport play, military history, substance use treatment history, psychologically traumatic event history, and BGLHA childhood score. Among 845 brain donors, the mean age at death was 60.3 (SD = 19.6) years. 589 donors (69.7%) had CTE, and 383 donors (45.3%) had a 1°FHMI. 1°FHMI was significantly associated with standardized adult BGLHA scores in those with CTE, but not in those without CTE (CTE present: β = 0.16, 95% CI 0.02-0.29; CTE absent: β = 0.10, 95% CI -0.12 to 0.32). The largest effects were observed among those with CTE, aged 40-59 years (CTE present: β = 0.64, 95% CI 0.32-0.96; CTE absent: β = 0.05, 95% CI -0.44 to 0.54), particularly for BGLHA factors of emotional dysregulation/impulsiveness (CTE present: β = 1.68, 95% CI 0.78-2.58; CTE absent: β = 0.09, 95% CI -1.20 to 1.37) and antisocial behavior (CTE present: β = 1.56, 95% CI 0.64-2.47; CTE absent: β = 0.10, 95% CI -1.19 to 1.40). Among brain donors exposed to repetitive head impacts, CTE pathology moderated the effect of 1°FHMI on BGLHA scores, with the largest effects in midlife. Predisposition to mental illness and CTE pathology may increase risk of aggression beyond each risk factor's additive effects. Prospective studies are needed to confirm these results.

The Association Between Social Media Addiction and Aggressive Behaviors: A Longitudinal and Gender-Specific Analysis.

Recent evidence demonstrates an association between social media addiction (SMA) and aggressive behaviors; however, the longitudinal relationship between these two variables remains not fully understood. The aim of this study was to examine the longitudinal relationship between SMA and aggressive behaviors (overt and relational aggression) in early adolescence and to identify gender differences in this relationship. A sample of 568 Italian early adolescents (52.3% girls; Mage = 12.59, SD = 0.87) attending middle schools in northern Italy was recruited from different schools. Participants completed an anonymous questionnaire to assess SMA and the perpetration of overt and relational aggressive behaviors. The questionnaire was completed twice with a 1-year intervals (T1 and T2). The findings showed that females were at higher risk of SMA and relational aggression, whereas males exhibited higher levels of overt aggression at both time points (T1 and T2). Longitudinal analyses revealed that SMA at T1 was associated with higher likelihoods of both overt and relational aggression at T2 across both genders and that only in females was relational aggression at T1 associated with higher levels of SMA at T2. The study suggests a relationship between SMA and aggressive behaviors and reveals gender differences in this pattern. Limitations, future directions, and practical implications of the research are discussed.

Introduction of the Broset Violence Checklist in the emergency department: A retrospective cohort study.

The Broset Violence Checklist (BVC) can stratify the risk of violence and aggression in EDs. The aim of the present study was to report the initial uptake of introducing this checklist and associations with unplanned alerts to potential or actual violence in two EDs. The BVC was recommended in all patient care episodes. This retrospective review included routinely collected data from an adult tertiary referral hospital and a suburban mixed paediatric and adult ED over a 12-month period. The primary outcome variable was completion of at least one BVC score and the secondary outcome was unplanned alerts to potential or actual violence episodes within the EDs. There were 121 330 presentations, of which 108 274 were included in the present study. The BVC was completed for 42 675 (39.4%) presentations. Using a cut-off score of 3, BVC had a specificity of 99.2% (95% confidence interval [CI] 99.1-99.2) and a sensitivity of 15.6% (95% CI 12.5-19.3) for unplanned alerts to potential or actual violence events. Completion of a BVC was independently associated with more unplanned alerts to potential or actual violence events (adjusted odds ratio 1.37; 95% CI 1.12-1.66). The BVC was highly specific for violence and aggression but had low sensitivity. Completion of the BVC was associated with more frequent unplanned alerts to potential or actual violence events, suggesting that high-risk patients might be identified intuitively, without formal scoring. Further exploration of the utility of the BVC in the ED is indicated with a focus on strategies to prevent violence and aggression.

Gastroprotective Effect of Hexanic Extract of Heliotropium indicum Against Ethanol-Induced Gastric Lesions in a CD1 Mouse Model.

Peptic ulcers result from an imbalance between protective factors (e.g., prostaglandins, nitric oxide, and sulfhydryl groups) and aggressive risk factors (e.g., consumption of non-steroidal anti-inflammatory drugs, alcohol, or tobacco) regarding the gastric mucosa. While various existing treatments aim to relieve pain, repair the ulcer, and prevent its recurrence, they often produce undesirable side effects. The Heliotropium indicum (H. indicum) plant has been utilized as a traditional medicine due to its gastroprotective activity. In this study, we identified the compounds responsible for the gastroprotective activity of the hexanic extract of H. indicum in an ethanol-induced damage model, in addition to determination of the activities of prostaglandins, nitric oxide, and non-protein sulfhydryl groups, along with the antisecretory and antioxidant activities (i.e., concentration of malondialdehyde and activities of the enzymes superoxide dismutase, catalase, and glutathione peroxidase). We found at least two groups of compounds that are responsible for this activity, namely 1-acyl-glycerol components and retinyl β-glucuronide derivatives. In conclusion, a mixture of compounds responsible for the gastroprotective activity of H. indicum was isolated from its hexanic extract, and non-protein sulfhydryl groups were implicated in its mechanism of action.

Factors shaping socio-emotional trajectories in sanctuary-living bonobos: a longitudinal approach.

Early maternal loss can have lasting detrimental effects on primate social development. While many rehabilitation settings provide enriching environments to buffer against such effects in orphans, previous research indicates that young bonobo (Pan paniscus) orphans exhibit striking deficiencies in socio-emotional competence compared to their mother-reared peers. However, such studies are generally cross-sectional, without accounting for changes across the lifespan. We conducted longitudinal observations in bonobos living in an accredited African ape sanctuary to examine how rearing background, sex and age predict social tendencies including affiliation, consolation and aggression risk. Affiliative tendencies increased in females and decreased in males with age but were overall lower in orphans compared to mother-reared bonobos. Consolation tendencies decreased with age in mother-reared bonobos, while orphans showed consistently lower consolation (akin to levels of older mother-reared individuals). Young and male bonobos were more likely to receive aggression, while mother-reared and older females were more likely aggressors. Our study highlights the potential that ape sanctuaries like this can have by demonstrating that orphans exhibit decreased affiliative tendencies yet show social functioning ranging within patterns of their mother-reared peers. We discuss these results in the context of bonobos' natural social ecology and ongoing rehabilitation efforts in this species.

Association Between Chronic Kidney Disease Risk Categories and Abdominal Aortic Calcification: Insights From the National Health and Nutrition Examination Survey.

Abdominal aortic calcification (AAC) is a critical indicator of cardiovascular risk, particularly in patients with chronic kidney disease (CKD). Traditional classification systems may underestimate the risk in those with moderate CKD. This study aimed to evaluate the association between CKD risk categories - defined by both estimated glomerular filtration rate (eGFR) and albuminuria - and the prevalence of severe AAC. This cross-sectional study analyzed data from the National Health and Nutrition Examination Survey (NHANES) 2013-2014. We included adults aged ≥ 40 years who underwent imaging for AAC assessment, excluding pregnant individuals and those without AAC scores. Survey-weighted and multivariate logistic regression was employed to assess the relationship between CKD risk categories and severe AAC, adjusting for age, hypertension, and smoking history. Subgroup analyses were conducted to explore variability across demographic and clinical subgroups. We analyzed data from 3,140 participants in the NHANES, 423 (13.4%) of whom had severe AAC. The cohort was categorized into CKD risk categories 1 through 4, with the majority (76%) in stage 1. Severe AAC was more prevalent among older individuals and those with traditional cardiovascular risk factors. Initial unadjusted analyses revealed that CKD category 2 was associated with a nearly fourfold increase in severe AAC (odds ratio (OR): 3.93), while categories 3 and 4 showed 3.75-fold and over 10-fold increases, respectively (all P < 0.01). However, after adjusting for confounders, categories 2 and 4 showed higher risks of severe AAC compared to category 1, but these associations did not reach statistical significance (OR: 1.72, 95% confidence interval (CI): 0.90 - 1.86, P = 0.06 and OR: 5.70, 95% CI: 0.85 - 38.00, P = 0.07, respectively). Our study offers insights that may complement the current reliance on eGFR and albuminuria in risk stratification, highlighting that CKD category 2, defined by mildly reduced eGFR and albuminuria, may be a potential marker for severe AAC. Although statistical significance was narrowly missed after full adjustment, the clinical implications remain significant, advocating for more aggressive cardiovascular risk management in this population. This understanding may contribute to evolving approaches in CKD-related cardiovascular risk assessment and inform potential intervention strategies.

Vietnam’s hedging amid U.S.-China Mekong rivalry: risk management under uncertainties

This paper examines how Vietnam has navigated U.S.-China rivalry in the Mekong basin while seeking to ensure its security, prosperity, and autonomy. I use the theoretical lens of hedging, defined as an insurance-maximising behaviour under conditions of high uncertainty. While existing scholarship has extensively examined Vietnam’s hedging on maritime security issues and the broader U.S.-China strategic and economic competition, this paper makes an empirical contribution by focusing on the relatively understudied angle of hedging in the Mekong basin context. I argue that Vietnam has hedged by (1) diversifying its Mekong partnerships with both the United States and China and (2) selectively cooperating with China under the Lancang-Mekong Cooperation. These acts aim to minimise Vietnam’s exposure to the long-term consequences of several non-traditional security risks, namely ecological challenges, water insecurity, economic vulnerabilities, and energy insecurity. Vietnam’s Mekong hedging also insures against the traditional security risks of big-power entrapment and military aggression as U.S.-China competition intensifies.

A study of asset and liability management applied to Brazilian pension funds

A study of asset and liability management applied to Brazilian pension funds