Gastroprotective Effect of Hexanic Extract of Heliotropium indicum Against Ethanol-Induced Gastric Lesions in a CD1 Mouse Model.

Abstract

Peptic ulcers result from an imbalance between protective factors (e.g., prostaglandins, nitric oxide, and sulfhydryl groups) and aggressive risk factors (e.g., consumption of non-steroidal anti-inflammatory drugs, alcohol, or tobacco) regarding the gastric mucosa. While various existing treatments aim to relieve pain, repair the ulcer, and prevent its recurrence, they often produce undesirable side effects. The Heliotropium indicum (H. indicum) plant has been utilized as a traditional medicine due to its gastroprotective activity. In this study, we identified the compounds responsible for the gastroprotective activity of the hexanic extract of H. indicum in an ethanol-induced damage model, in addition to determination of the activities of prostaglandins, nitric oxide, and non-protein sulfhydryl groups, along with the antisecretory and antioxidant activities (i.e., concentration of malondialdehyde and activities of the enzymes superoxide dismutase, catalase, and glutathione peroxidase). We found at least two groups of compounds that are responsible for this activity, namely 1-acyl-glycerol components and retinyl β-glucuronide derivatives. In conclusion, a mixture of compounds responsible for the gastroprotective activity of H. indicum was isolated from its hexanic extract, and non-protein sulfhydryl groups were implicated in its mechanism of action.