HIV Risk Group Research Articles

Tuberculosis among people with HIV infection in the United Kingdom: opportunities for prevention?

To investigate the incidence of, and risk factors for, tuberculosis among HIV clinic attendees in the United Kingdom. Observational cohort study of 27 868 individuals in the United Kingdom Collaborative HIV Cohort collaboration, 1996-2005. Among individuals not taking combination antiretroviral therapy (cART), tuberculosis incidence was considerably higher among individuals of black African vs. white or other ethnicities {incidence rates 9.9 [95% confidence intervals (CIs) 7.2, 12.6], 2.5 [95% CI 1.8, 3.0] and 4.4 [95% CI 2.7, 6.0] episodes per 1000 person-years, respectively}. Tuberculosis incidence decreased with time after starting cART; among black Africans, incidence was consistently higher and remained substantial (5.3 per 1000 person-years) at 24 months and longer after starting cART. The strongest independent risk factors for tuberculosis after cART start were most recent CD4 cell count: adjusted rate ratios (aRR) 10.65 (95% CI 6.11, 18.57), 3.40 (95% CI 2.05, 5.65), 1.77 (95% CI 1.06, 2.96) and 1.84 (95% CI 1.09, 3.12) for individuals with CD4 cell counts less than 50, 50-199, 200-349 and 350-499 cells/microl, respectively, compared with at least 500 cells/microl; and black African vs. white ethnicity [aRR 2.93 (95% CI 1.89, 4.54)]. HIV risk group, shorter time on cART, later calendar period and unsuppressed viral load were also independently associated with incident tuberculosis. Tuberculosis incidence among people attending UK HIV clinics is substantial, particularly among those with non-white ethnicity and low CD4 cell counts, even after starting cART. Earlier HIV diagnosis is needed in order to implement interventions to prevent tuberculosis; tuberculosis preventive therapy should be considered in addition to cART.

Early mortality and cause of deaths in patients using HAART in Brazil and the United States

To compare the early mortality pattern and causes of death among patients starting HAART in Brazil and the United States. We analyzed the combined data from two clinical cohorts followed at the Johns Hopkins AIDS Service in Baltimore, United States, and the Evandro Chagas Clinical Research Institute AIDS Clinic in Rio de Janeiro, Brazil. Participants included those who entered either cohort between 1999 and 2007 and were antiretroviral naive. Follow-up was at 1 year since HAART initiation. Cox proportional hazards regression analysis was used to assess the role of the city on the risk of death. A total of 859 and 915 participants from Baltimore and Rio de Janeiro, respectively, were included. In Rio de Janeiro, 64.7% of deaths occurred within 90 days of HAART initiation; in Baltimore, 48.9% occurred between 180 and 365 days. AIDS-defining illness (61.8%) and non-AIDS-defining illness (55.6%) predominated as causes of death in Rio de Janeiro and Baltimore, respectively. Risk of death was similar in both cities (hazard ratio 1.04; P value = 0.95) after adjusting for CD4 T cell count, age, sex, HIV risk group, prior AIDS-defining illness, and Pneumocystis jirovecii pneumonia and Mycobacterium avium prophylaxis. Individuals with CD4 T cell count less than or equal to 50 cells/microl (hazard ratio 4.36; P = 0.001) or older (hazard ratio, 1.03; P = 0.03) were more likely to die. Although late HIV diagnosis is a problem both in developed and developing countries, differences in the timing and causes of deaths clearly indicate that, besides interventions for early HIV diagnosis, different strategies to curb early mortality need to be tailored in each country.

Molecular detection ofT. pallidumby PCR in seronegative cases

For the molecular detection of Treponema pallidum authors introduced and used a nested PCR amplifying a conservative portion of the gene coding for the Tp 47 kDa membrane protein. PCR verified the presence of T. pallidum specific DNA in 5.7 per cent of syphilis seronegative 105 MSM belonging to HIV risk group. Treponema DNA was also detected in HIV infected, syphilis seronegative cases. Specificity of the method was demonstrated in rabbit inoculation test and also in clinically positive syphilis cases.

A cluster analysis of drug use and sexual HIV risks and their correlates in a sample of African-American crack cocaine smokers with HIV infection

The purpose of this cross-sectional study was to classify a sample of HIV-seropositive African-American crack cocaine smokers into homogenous HIV drug use and sexual risk groups using a two step multivariate cluster analysis. Two hundred and fifty-eight crack cocaine smokers participated in the study. Cluster analysis revealed three distinct HIV risk groups. The highest risk group, the largest one, was characterized by frequent, daily crack use, multiple sex partners, trading sex, and inconsistent condom use. The consistent condom use group, the smallest group, was characterized by consistent condom use. The inconsistent condom use group, the second largest group, was distinguished by inconsistent condom use. Comparisons of the three HIV risk groups revealed that the highest risk group had a higher proportion of illegal sources of income, higher proportion of binged crack use, frequent, daily, alcohol use, same gender sex partners, and scored higher on depressive symptoms. Members of the consistent condom use group were more likely to have been HIV diagnosed for a shorter time, to have HIV serodiscordant casual sex partners, higher psychological motivation for condom use, and a lower frequency of vaginal sex. Members of the inconsistent condom use group were more likely to have a main sex partner, to be married, to be on public assistance, to know the HIV serostatus of their casual partner, and less likely to conceal their HIV serostatus. An alarming finding was that a large number of participants inconsistently used condoms with HIV serodiscordant sex partners. Interventions aiming to prevent the secondary spread of HIV infection in African-American crack cocaine smokers should take this variability in account and focus on the differences.

Sex Work and HIV Status Among Transgender Women

Transgender women are a key risk group for HIV, and epidemiologic studies have attributed high rates of HIV infection to behaviors associated with sex work in this population. This systematic review compared HIV prevalence among transgender female sex workers (TFSWs) with prevalence among transgender women who do not engage in sex work, male sex workers, and biologically female sex workers. We conducted systematic searches of 6 electronic databases, and including studies that met pre-established criteria. We extracted data, appraised methodologic quality, assessed heterogeneity, and organized meta-analyses by comparison group. We identified 25 studies among 6405 participants recruited from 14 countries. Overall crude HIV prevalence was 27.3% in TFSWs, 14.7% in transgender women not engaging in sex work, 15.1% in male sex workers, and 4.5% in female sex workers. Meta-analysis indicated that TFSWs experienced significantly higher risk for HIV infection in comparison to all other groups (relative risk [RR] = 1.46, 95% confidence interval [CI]: 1.02 to 2.09), and particularly in comparison to female sex workers (RR = 4.02, 95% CI: 1.60 to 10.11). We observed significant heterogeneity among the included studies, along with methodologic limitations and imprecise definitions of sex work and gender. TFSWs could benefit from targeted HIV prevention interventions, HIV testing, and interventions to help reduce the risk of contracting or transmitting HIV. Structural interventions to reduce reliance on sex work among transgender women may be warranted.

Sex worker incarceration in the People’s Republic of China

Tens of thousands of commercial sex workers in China are administratively detained each year in female re-education through labor (RTL) centres for moral education and vocational training. Recent increases in...

Comparative assessment of coronary risk in HIV-infected patient cohort using Framingham index and Spanish validated coronary risk index: REGICOR

Methods We estimated the 10-year coronary risk in a sample of Spanish outpatients in our HIV clinic, using the standard Framingham index and an index developed and validated in a population of Catalonia (Northern Spain): REGICOR. The following data were collected in all patients: age, gender, smoking status, HIV risk group, CD4+ cell count, HIV viral load, fasting glucose and insulin levels, lipid profile, blood pressure, waist circumference, BMI and present ART. We used the HOMA (homeostatic model assessment) index to assess insulin resistance, choosing as cut-off point, 3.8. The IDF (International Diabetes Foundation) criteria have been used to define increased waist circumference, hypertension, hypertrygliceridemia, low HDL-cholesterol and hyperglycemia.

Contribution of antiretroviral therapy, cardiovascular risk factors and constituents of metabolic syndrome to insulin resistance(IR) in HIV

Methods We studied all patients who had a visit in our outpatient HIV clinic during 2007. The following data were collected: age at diagnosis of HIV infection, gender, country of origin, smoking status, HCV co-infection, HIV risk group, AIDS diagnosis, CD4+ cell count, HIV viral load, fasting glucose and insulin levels, lipid profile, blood pressure, waist circumference, BMI and present HAART. We used the HOMA index to assess insulin resistance, choosing as cut-off point 3.8. The IDF criteria have been used to define increased waist circumference, hypertension, hypertrygliceridemia, low HDL cholesterol and hyperglycemia. We assessed the association of these variables with IR using a multivariate logistic regression predictive model. Blood glucose value was not taken into account in the analysis since it is embedded in the HOMA index. Discriminating power of the model was analyzed by means of a receiver operating curve (ROC). The effect measure was the adjusted odds-ratio with 95% confidence interval (CI).

Type of Substance Use and Access to HIV-Related Health Care

HIV-infected substance users have poorer health outcomes than other HIV risk groups. Few studies have examined the impact of specific types of substance use on health care. This study investigated the associations between specific types of substances of abuse and access to health care. HIV-infected individuals living in eight different single room occupancy hotels in the Bronx, New York, were interviewed between August 1999 and February 2001 regarding demographics, health care access and utilization, and drug and alcohol use. Of the 238 participants, the majority were male (59%), black or Hispanic (93%), and active drug users (61%). Individuals reporting any drug or crack/cocaine use were less likely to have a regular provider than those reporting no drug or no crack/cocaine use (adjusted odds ratio [AOR] = 0.50, p = 0.05; AOR =0.35, p = 0.004, respectively), while those with binge alcohol use were more likely to have a regular doctor than those without binge alcohol use (AOR = 2.61, p = 0.05). Individuals reporting any drug or crack/cocaine use were also less likely to perceive quality of health care positively (AOR = 0.50, p = 0.02; AOR = 0.37, p = 0.002, respectively). Heroin use, and injection drug use were not associated with these outcomes. When the sample was limited to recent drug users, similar patterns were found. Although drug use in general is associated with negative health outcomes, in this study, poorer measures of access to health care among substance users was associated predominantly with crack/cocaine use. It is important that clinicians and researchers working with substance-using populations understand how specific types of substance use differentially impact on health care.

Séroprévalence de l'hépatite A chez des patients infectés par le VIH

The authors had for aim to prospectively study the hepatitis A seroprevalence of an HIV-infected population, followed-up in an outpatient clinic (CISIH Strasbourg). Blood tests were performed on all patients from September 2003 to March 2004 to screen for hepatitis A (total antibodies with Elisa). The overall seroprevalence was 219/514 (56.6%), similar in male and female patients. It increased with age, especially in European patients (P = 0.003). The seroprevalence was lower in European subjects: 46.3% (while it reached 100% in sub-Saharan Africans), the prevalence was similar whatever the HIV risk group (46% in homosexual as well as in heterosexual patients, 44% in intravenous drug users). Hepatitis B or C co-infection did not increase the seroprevalence of hepatitis A. The hepatitis A seroprevalence was similar in various CD4 T cell count categories. Our results stress the utility of hepatitis A serology in HIV-infected patients (more than 50% of European patients are non immune), and the importance of assessing hepatitis A vaccination.

Cost-Effective Allocation Of Government Funds To Prevent HIV Infection

Relative to the magnitude of the epidemic, government funds available for HIV prevention are scarce. To optimize use of funds, we applied a mathematical model of the cost of HIV prevention interventions using national data on HIV risk-group size and HIV prevalence. This procedure suggested an allocation of funds across nine interventions to potentially prevent an estimated 20,000 infections annually, compared with the estimated 7,300 infections potentially prevented through four interventions now recommended by the Centers for Disease Control and Prevention (CDC). The optimal allocation will involve a combination of intensive interventions for high-prevalence populations and inexpensive large-scale interventions for lower-prevalence populations.

Surplus men, sex work, and the spread of HIV in China

While 70% of HIV positive individuals live in sub-Saharan Africa, it is widely believed that the future of the epidemic depends on the magnitude of HIV spread in India and China, the world's most populous countries. China's 1.3 billion people are in the midst of significant social transformation, which will impact future sexual disease transmission. Soon approximately 8.5 million 'surplus men', unmarried and disproportionately poor and migrant, will come of age in China's cities and rural areas. Meanwhile, many millions of Chinese sex workers appear to represent a broad range of prices, places, and related HIV risk behaviors. Using demographic and behavioral data, this paper describes the combined effect of sexual practices, sex work, and a true male surplus on HIV transmission. Alongside a rapid increase in sexually transmitted disease incidence across developed parts of urban China, surplus men could become a significant new HIV risk group. The anticipated high sexual risk among many surplus men and injecting drug use use among a subgroup of surplus men may create bridging populations from high to low risk individuals. Prevention strategies that emphasize traditional measures--condom promotion, sex education, medical training--must be reinforced by strategies which acknowledge surplus men and sex workers. Reform within female sex worker mandatory re-education centers and site specific interventions at construction sites, military areas, or unemployment centers may hold promise in curbing HIV/sexually transmitted infections. From a sociological perspective, we believe that surplus men and sex workers will have a profound effect on the future of HIV spread in China and on the success or failure of future interventions.

Ethnic differences in stage of presentation of adults newly diagnosed with HIV‐1 infection in south London

To establish whether there were ethnic differences in demographic characteristics, the stage at HIV diagnosis and reasons for and location of HIV testing between 1998 and 2000 in a large ethnically diverse HIV-1-infected clinic population in south London in the era of highly active antiretroviral therapy. A retrospective review was carried out of all persons >18 years old attending King's College Hospital with a first positive HIV-1 test between 1 January 1998 and 31 October 2000, and of a random sample of patients attending St Thomas' hospital with a first positive HIV-1 test in the same period. Demographic data, details of reasons for and site of HIV test, clinical stage, CD4 lymphocyte count and HIV-1 viral load at HIV diagnosis were abstracted from the local database and medical records. Comparisons were made according to ethnic group (white, black African and black Caribbean) and over time (1998, 1999 and 2000). Of the 494 patients with new HIV-1 diagnoses between January 1998 and December 2000, 179 (36.2%) were white, 270 (54.7%) were black African and 45 (9.1%) were black Caribbean. There were significant differences across the ethnic groups in HIV risk group, reasons for and site of HIV testing, and clinical and CD4 stage at diagnosis. Among whites, 72.6% were men who had sex with men, 3.4% injecting drug users and 21.2% heterosexuals, compared to 2.2%, 0.4% and 93.3% among black Africans, and 28.9%, 0% and 68.9% among black Caribbeans (P<0.001). Black Africans were more likely to present with an AIDS diagnosis (21.3%) and a lower CD4 cell count [223 cells/microL; interquartile range (IQR) 88-348] compared to both whites (9.9%; 358 cells/microL; IQR 151-508) and black Caribbeans (17.9%; 294 cells/microL; IQR 113-380), who were intermediate between whites and black Africans in their stage of presentation. There was a statistically nonsignificant trend with time, between 1998 and 2000, towards earlier diagnosis based on the CD4 cell count in whites (323 and 403 cells/microL) and black Caribbeans (232 and 333 cells/microL), but a later diagnosis in black Africans (233 and 175 cells/microL). The majority of black Africans were HIV-tested as a result of suggestive symptoms or antenatal screening (58.4%) rather than because of perceived risk (40.5%), in contrast to the situation in whites (24.1% vs. 71.7%, respectively) or black Caribbeans (34.5% vs. 65.5%, respectively) (P<0.001). We found no significant differences across ethnic groups in age, HIV-1 viral load or year of HIV diagnosis. Black Africans continue to present with more advanced HIV disease than whites or black Caribbeans, with no evidence of any trend towards earlier diagnosis. Future educational campaigns designed to promote the uptake of HIV testing among black Africans and black Caribbeans will need to address the multiple barriers to testing, including misperception of risk, stigma and ready access to testing.

Identifying diverse HIV risk groups among American Indian young adults: the utility of cluster analysis.

We demonstrate the utility of cluster analysis for identifying diverse HIV risk groups found in a community-based sample. Within a group of 706 American Indian young adults, we used cluster analysis to identify four profiles of HIV risk/protection. The High Efficacy/Low Risk cluster had high levels of knowledge/education, self-efficacy, and outcome expectations about HIV protection, with low levels of risk behaviors. Low Efficacy/Low Risk had low levels of HIV knowledge/education, self-efficacy, and outcome expectations, but high levels of perceived risk for HIV with low levels of HIV risk behaviors. Low Efficacy/Moderate Risk was similar to the previous group, but its members had moderately higher levels of several risk behaviors and higher condom use. Low Efficacy/High Risk had high rates of several high-risk behaviors such as exchanging sex for money or injection drug use. Validation analyses highlighted differences that can be useful for the development of preventive interventions.

Drug Injection and Sexual Mixing Patterns of Drug-Using Male Sex Workers

Drug-using male sex workers (DUMSWs) are known to have large numbers of drug injection and sex partners. The purpose of this study was to describe the assortative and disassortative drug injection and sexual mixing patterns of DUMSWs. Implications of the high rates of disassortative mixing patterns of DUMSWs for HIV infection are discussed. Implications of disassortative mixing of DUMSWS with regard to DUMSWs bridging disparate HIV risk groups are evaluated. Data were collected from 89 DUMSWS. Data on up to six drug injection and six sex partners were collected from respondents. One hundred drug injection and 169 sex respondent/partner pairs were analyzed for the proportions of pairs that were concordant (like) or discordant (unlike) in gender, trading sex for money, race/ethnicity, and age cohort. For race/ethnicity and age, within-group differences were assessed with chi-square statistics. Data showed high proportions of discordant respondent/partner pairs for both drug injection and sex by gender, trading sex for money, race/ethnicity, and age. Significant within-group differences in rates of discordant pairs were found for both behaviors in relation to race/ethnicity and age. Minority persons and respondents 19 years of age or younger were more likely to be in discordant pairs. Direct assessment of HIV risk posed by the mixing patterns was not possible. Elaborating the mechanisms by which DUMSWs might act as an epidemiologic bridge is complex and involves more variables than were explored in this study. Nevertheless, such a study would be worthwhile.

Use of complementary and alternative medicine by HIV-infected outpatients in Ontario, Canada.

Little is known about complementary and alternative medicine (CAM) use in Canadian patients with HIV. We sought to determine the prevalence of CAM use by patients attending HIV outpatient clinics in Ontario, Canada, and to compare the characteristics of users and nonusers. Impact of CAM definition on reported utilization rates was also assessed, specifically in relation to the inclusion and exclusion of vitamins, minerals, and multivitamins in CAM definition. In-person interviews were conducted between 1999 and 2001 with 104 HIV-positive outpatients enrolled in the HIV Ontario Observational Database project (HOOD) and attending HIV outpatient clinics in Ontario. Self-reported CAM utilization and demographic data were collected. Clinical data were obtained from medical chart review. Seventy-seven percent of participants reported current CAM use. Inclusion of vitamins and minerals (CAMVIT) increased this estimate to 89%. Nearly all patients used CAM in conjunction with antiretroviral medications. Out of pocket costs ranged from CAD$0 to more than CAD$250 per month. Most patients reported CAM use was beneficial and had improved their overall health. Female gender, HIV risk group, number of prescriptions, and overall number of drugs used were associated with CAM use. CAM use in Canadian patients with HIV is extremely common, with higher use among women. The definition of CAM has a substantial impact both on reported prevalence rates and on predictors of CAM use.

Psychocultural Factors Associated with HIV Infection Among Trinidad and Tobago Adolescents

SUMMARY The Caribbean region ranks highest in terms of reported HIV incidence in the Americas and is second in the world. The islands of Trinidad and Tobago are disproportionately affected by HIV compared with the rest of the English-speaking Caribbean. In the twin-island state of Trinidad and Tobago, the fastest growing HIV risk group is adolescents ages 15 to 24. Among this group, females are especially vulnerable. This article explores the complex cultural, economic, and social factors obscuring the reduction of HIV infection among adolescents. Special emphasis is placed on psychocultural factors that leave female adolescents especially vulnerable. Implications and recommendations are made for developing effective HIV prevention programs by highlighting the role of culture-specific community-based resources for these at-risk groups.

Substance-abusing adolescents at varying levels of HIV risk: psychosocial characteristics, drug use, and sexual behavior.

To evaluate the relationship of various psychosocial factors on HIV sexual risk behavior in a sample of 169 "inner city" male and female adolescents mandated into in court-ordered substance abuse treatment. The Millon Adolescent Clinical Inventory (MACI) and measures of sexual behavior, condom attitudes and skills, HIV knowledge, and substance abuse were administered. Data were evaluated according to five HIV risk groups: abstinent (n = 37); monogamous and practicing only protected sex (n = 19); monogamous and practicing unprotected sex (n = 45); multiple partners and practicing only protected sex (n = 11); and multiple partners and having only unprotected sex (n = 57). Significant main effects were found for impulsive propensity, submissiveness, marijuana and alcohol use, condom attitudes, and intentions to engage in safer sex. Protective behavior was directly associated with submissiveness and inversely associated with impulsive personality profiles, with increased marijuana use emerging as a significant predictive factor in the choice for sexual activity vs. abstinence. More alcohol use was predictive of choosing multiple partners vs. monogamy. Factoring risk variation into the design of HIV psychosocial research may enhance the tailoring of effective prevention strategies.

The correlates and predictive validity of HIV risk groups among drug users in a community-based sample: methodological findings from a multi-site cluster analysis

Outreach and intervention with out-of-treatment drug users in their natural communities has been a major part of our national HIV-prevention strategy for over a decade. Intervention design and evaluation is complicated because this population has heterogeneous patterns of HIV risk behaviors. The objectives of this paper are to: (a) empirically identify the major HIV risk groups; (b) examine how these risk groups are related to demographics, interactions with others, risk behaviors, and community (site); and (c) evaluate the predictive validity of these risk groups in terms of future risk behaviors. Exploratory cluster analysis of a sample of 4445 out-of-treatment drug users from the national data set identified eight main risk subgroups that could explain over 99% of the variance in the 20 baseline indices of HIV risk. We labeled these risk groups: Primary Crack Users (29.2%), Cocaine and Sexual Risk (12.8%), High Poly Risk Type 2 (0.3%), Poly Drug and Sex Risk (10.9%), Primary Needle Users (24.1%), High Poly Risk Type 1 (1.4%), High Frequency Needle Users (19.8%), and High Risk Needle Users (1.6%). Risk group membership was highly related to HIV characteristics (testing, sero-status), demographics (gender, race, age, education), status (marital, housing, employment, and criminal justice), prior target populations (needle users, crack users, pattern of sexual partners), and geography (site). Risk group membership explained 63% of the joint distribution of the original 20 HIV risk behaviors 6 months later (ranging from 0.03 to 37.2% of the variance individual indices). These analyses were replicated with both another 25% sample from the national data set and an independent sample collected from a new site. These findings suggest HIV interventions could probably be more effective if they targeted specific subgroups and that evaluations would be more sensitive if they consider community and sub-populations when evaluating these interventions.

BIOLOGIC APPROACHES TO THE PREVENTION OF SEXUAL TRANSMISSION OF HUMAN IMMUNODEFICIENCY VIRUS

HIV prevention science has made progress, especially in Thailand and some sub-Saharan African countries. New cases of HIV in the United States, however, have not diminished and explosive epidemics in India and the People's Republic of China seem inevitable. Therefore, HIV prevention activities must move forward in parallel. Funding for biologic and behavioral research efforts must be balanced. Behavioral research must inform biologic strategies. In addition, HIV prevention efforts have been distorted by forces that require further consideration. First, the stigmatization associated with a diagnosis of HIV infection led to prevention efforts that virtually ignore the index case. Focusing entirely on the susceptible population puts intense and unrealistic pressure on behavior change and vaccine development. Although development of an HIV vaccine is desirable, there is no evidence that this goal can be achieved in the near future. Blind faith in vaccine technology detracts from pursuit of alternative aspects of prevention science. Vaccine development is but one of several key components to a broad-based prevention strategy. The history of control of infectious diseases has shown the need for targeting index cases. This certainly will prove important in HIV over the next few years. In developed countries, antiretroviral therapy for established HIV infection has become the standard of care. Increased knowledge of the biology of transmission of HIV suggests use of ART to prevent transmission. Such intervention must be accompanied by safer sex behavior in the index cases, and ultimately could lead to some form of monitoring and directly observed therapy. At this time, the latter approach seems unrealistic in developing countries, where the expense of drugs renders them unavailable. But there is every reason to believe that cheaper, more appropriate drugs will be developed before an effective vaccine. Furthermore, targeted use of ART might have disproportionate benefits in some countries. Women are the fastest growing HIV risk group. Several issues, both biologic and social, make this trend a concern. Increases in the number of HIV-infected women will lead to greater vertical transmission. Women possibly have different risk factors for acquisition and transmission than men. Information about the effects of vaginal ecology, specifically, the role of bacterial vaginosis, in the acquisition of HIV is essential because bacterial vaginosis can be reversed, at least transiently. To allow women to take an active role in HIV prevention methods, development of a topical microbicide is vital and may prove easier than a vaccine. Finally, HIV prevention efforts require knowledgeable, central leadership. All prevention efforts should be developed and implemented in parallel, to gain a synergistic result. Few vaccine experts are enthusiastic about microbicides, and HIV caregivers only rarely focus on the public health considerations of their patients. Stopping the spread of HIV requires a coordinated, concerted efforts using "all the tools in the toolbox."