Rice-wheat Rotation Research Articles

Straw management adopted by large farms sustains grain yield but mitigates greenhouse gas emissions

ContextStraw incorporation is a globally recommended strategy for enhancing soil fertility. The methods for straw incorporation adopted by large farms usually differ from those used by small households, primarily because of variations in cost affordability. However, the effects of straw management on rice yields and greenhouse gas (GHG) emissions have not been well explored at different farming scales. ObjectivesThis study aims to compare the grain yield and greenhouse gas emissions under different straw management adopted by large farms than small households. MethodsA three-year field experiment using split-plot design, with straw management as the main plot and preceding tillage as the sub-plot, was conducted in a wheat-rice rotation system. Four treatments were given as rotary tillage with straw removal (RT-S), rotary tillage with straw incorporation (RT+S; small households), ploughing tillage with straw removal (PT-S), and ploughing tillage with straw incorporation (PT+S, large farms) in the preceding wheat season. Soil tillage in the rice season was the same, which was rotary tillage after irrigation. ResultsStraw incorporation significantly increased methane (CH4) and nitrous oxide (N2O) emissions, and 60%-62% of CH4 was emitted during the transplanting to jointing stages while 45–73% of N2O was emitted during the jointing to the booting stage. The stimulation of CH4 and N2O emissions by straw incorporation was closely correlated to enhanced soil organic carbon (SOC) and denitrification enzyme activity, respectively. In the last year, PT+S significantly reduced the global warming potential (GWP) compared to RT+S in the whole rice season, which can be attributed to the dramatically decline of CH4 emission, even though N2O emission was increased. Additionally, the decreased CH4 emission was mainly due to lower methanogenic activity, and the increased N2O emission was mainly resulted from lower nosZ abundance under PT+S compared to RT+S. Straw incorporation increased rice yield by 15.73% on average in the last two years, and rice yield of PT+S in the last year was significantly enhanced by 27.50%, resulting in a lower greenhouse gas intensity (GHGI) by 25.54% compared to RT+S. ConclusionStraw management practices adopted by large farms (PT+S) have the potential to synchronize high rice yields with lower GHG emissions compared to small households (RT+S). ImplicationsOur findings provide some evidence that large-scale agricultural organization may be helpful for sustainable rice production in China.

Effect of Different Boron Application Schedules on Yields, Boron Concentration and Uptake of Rice-Wheat Rotation and Changes in Chemical Fractions of Soil Boron

ABSTRACT The yields realized under rice-wheat rotation are generally low in the geographical regions prone to B deficiency and a sound B application schedule recommendation is required for the sustainable yields and enhanced soil fertility. The effect of different B application schedules involving different dosages and frequency of B application was investigated on yields and B nutrition of rice-wheat rotation and changes in the chemical fractions of B in soil in six years field study carried out on a sandy loam (Typic hapludoll). Based on the average grain yields of rice and wheat during the period of experimentation, application of 1.5 kg B as borax ha−1 to rice crop on alternate years was the profitable practice for mollisols as it gave an average yearly production of 5.51 t of rice grain and 4.28 t of wheat grain ha−1. The average concentration of B in flag leaves and grains of rice was much lower compared to wheat crop. The recommended schedule of B application to rice-wheat rotation resulted in a Benefit: Cost ratio of 11: 1 and also maintained the optimum level of B in soil. Most of the added B markedly increased specifically bound (0.05 M KH2PO4 extractable), ammonium oxalate (pH 3.3) extractable fractions followed by residual and organically bound fraction in the soil.

Long‐term successive biochar application increases plant lignin and microbial necromass accumulation but decreases their contributions to soil organic carbon in rice–wheat cropping system

AbstractBiochar application is widely recognized as an effective approach for increasing soil organic carbon (SOC) and mitigating climate change in agroecosystems. However, the effects of biochar application on net accumulations and relative contributions of different SOC sources remain unclear. Here, we explored the effects of biochar application on plant‐derived (PDC) and microbial necromass C (MNC) in a 10‐year experimental rice–wheat rotation field receiving four different intensities of biochar application (0, 2.25, 11.5, and 22.5 t ha−1 for each crop season), using phospholipid fatty acids (PLFAs), lignin phenols and amino sugars as biomarkers of microbial biomass, PDC and MNC, respectively. Our results showed that biochar application increased SOC content and stock by 32.6%–203% and 26.4%–145%, respectively. Higher biochar application (11.5 and 22.5 t ha−1) increased soil pH, total nitrogen (TN), total phosphorus (TP), SOC/TN, and root biomass. In addition, higher biochar application enhanced bacterial, fungal, and total microbial biomass. Plant lignin phenols and MNC contents significantly increased, whereas their contributions to SOC significantly decreased with the increase in biochar application rates due to the disproportionate increase in PDC and MNC, and SOC. Fungal necromass had a greater contribution to SOC than bacterial necromass. The fungal/bacterial necromass decreased from 2.56 to 2.26 with increasing biochar application rates, because of the higher abundances of bacteria than that of fungi as indicated by PLFAs under higher biochar application rates. Random forest analyses revealed that pH, TP, and SOC/TN were the main factors controlling plant lignin and MNC accumulation. Structural equation modeling revealed that biochar application increased lignin phenols by stimulating root biomass, whereas enhanced MNC accumulation was primarily from increased microbial biomass and lignin phenols. Overall, our findings suggest that biochar application increases the accumulation of the two SOC sources but decreases their contributions to SOC in paddy soils.

Improving soil organic carbon mapping in farmlands using machine learning models and complex cropping system information

Obtaining accurate spatial maps of soil organic carbon (SOC) in farmlands is crucial for assessing soil quality and achieving precision agriculture. The cropping system is an important factor that affects the soil carbon cycle in farmlands, and different agricultural managements under different cropping systems lead to spatial heterogeneity of SOC. However, current research often ignores differences in the main controlling factors of SOC under different cropping systems, especially when the cropping pattern is complex, which is not conducive to farmland zoning management. This study aims to (i) obtain the spatial distribution map of six cropping systems by using multi-phase HJ-CCD satellite images; (ii) explore the stratified heterogeneous relationship between SOC and environmental variables under different cropping systems by using the Cubist model; and (iii) predict the spatial map of SOC. The Xiantao, Tianmen, and Qianjiang cities, which are the core agricultural areas of the Jianghan Plain, were selected as the study area. Results showed that the SOC content in rice–wheat rotation was the highest among the six cropping systems. The Cubist model outperformed random forest, ordinary kriging, and multiple linear regression in SOC mapping. The results of the Cubist model showed that cropping system, climate, soil attributes, and vegetation index were important influencing factors of SOC in farmlands. The main controlling factors of SOC under different cropping systems were different. Specifically, summer crop types had a greater influence on spatial variations in SOC than winter crops. Paddy–upland rotation was more affected by river distance and NDVI, while upland–upland rotation was more affected by irrigation-related factors. This work highlights the differentiated main controlling factors of SOC under different cropping systems and provides data support for farmland zoning management. The Cubist model can improve the prediction accuracy of SOC under complex cropping systems.

Straw Addition Enhances Crop Yield, Soil Aggregation, and Soil Microorganisms in a 14-Year Wheat-Rice Rotation System in Central China.

Straw return utilizes waste resources to reduce the use of chemical fertilizers worldwide. However, information is still lacking on the relative impact of straw return on soil fertility, the nutrient composition of different soil aggregates, and soil microbial communities. Therefore, this study aimed to understand the effects of different management practices on the crop yield, soil fertility, and soil community composition in a 14-year wheat-rice rotation system. The treatments included a control (without fertilizer and straw addition), chemical fertilization (NPK), straw return without fertilizer (S), and straw addition with chemical fertilizer (NPKS). The results showed that NPKS improved the wheat and rice yield by 185.12% and 88.02%, respectively, compared to the CK treatment. Additionally, compared to the CK treatment, the N, P, and K contents of the wheat stem were increased by 39.02%, 125%, and 20.23% under the NPKS treatment. Compared to the CK treatment, SOM, TN, TP, AN, AP, AK, CEC, AFe, AMn, ACu, and AZn were increased by 49.12%, 32.62%, 35.06%, 22.89%, 129.36%, 48.34%, 13.40%, 133.95%, 58.98%, 18.26% and 33.33% under the NPKS treatment, respectively. Moreover, straw addition promoted the creation and stabilization of macro-aggregates in crop soils. The relative abundance of macro-aggregates (0.25-2 mm) increased from 37.49% to 52.97%. Straw addition was associated with a higher proportion of aromatic and carbonyl carbon groups in the soil, which, in turn, promoted the formation of macro-aggregates. Redundancy analysis showed that straw return significantly increased the microbial community diversity. These findings demonstrate that straw addition together with chemical fertilizer could increase the crop yield by improving soil fertility, soil aggregate stability, and the diversity of fungi.

Partial Substitution of Chemical N with Solid Cow Manure Improved Soil Ecological Indicators and Crop Yield in a Wheat–Rice Rotation System

Alternative fertilizers are essential to minimizing the deteriorating effects of chemical fertilizers on soil and water quality/health. Accordingly, the present work investigated the effects of combined organic–inorganic fertilization (COIF) on wheat and rice yields, soil nutrients, and soil Cd accumulation. Hence, seven different treatments were set up: control (CK); conventional fertilization (CF); adequate fertilization (OF); organic fertilizer replacing 25% (T1) and 50% (T2) of OF; and organic nitrogen (N) replacing 25% (M1) and 50% (M2) of OF-N. Overall, significant increases occurred in the yields of COIF crops. Compared with the CF, the highest wheat and rice yields happened in the M1 treatment (with a difference of approximately 18.5%) (p < 0.05). COIF slightly alleviated soil acidification, and improved the cation exchange capacity (CEC) of the study soils. Furthermore, COIF treatments significantly increased the contents of total phosphorus, total potassium, available phosphorus, and available potassium by 6.35 to 16.9%, 3.17 to 10.9%, 5.53 to 28.7%, and 2.6 to 12%, respectively (p < 0.05). Nevertheless, negligible increases took place in the Cd content of COIF soils compared with that of the CK. Altogether, our results concluded that 25% replacement of OF-N by organic N (M1) effectively improved the fertility/ecological sustainability of the study soils.

Exogenous carbon addition soil mediated phosphorus dynamics under eight years continuous input of swine manure in a wheat-rice rotation

The study investigated the prolonged impact of swine manure application on soil inorganic phosphorus (P) fractions over an 8-year continuous, randomized field trial involving rotating wheat (wet conditions) and rice (flooded conditions) crops. The trial comprised six treatments: triplicate control plots receiving only chemical fertilizers, and triplicate plots receiving chemical fertilizers and/or swine manure ranging from 150 to 1200 kg P2O5 ha−1. The continuous application of swine manure increased soil P content and availability. Initially, P primarily accumulated in the soil as Fe-P during the first four years of manure application, transitioning to Ca8-P over the following four years. The main driver of these changes in P fractions was soil total organic carbon (TOC) was identified as the main driver of these changes in P fractions, contributing 31.5% to the observed variations. TOC increased steadily throughout the trial, with a faster rising in years 1–4 compared to years 4–8. Laboratory-scale soil incubation experiments were conducted, involving the addition of glucose or cellulose as exogenous carbon sources to test their effects on soil P dynamics and mitigate environmental damage from P leaching. The addition of cellulose to soil that had received high quantities of swine manure for eight years resulted in increased the moderately labile and moderately resistant organic P fractions while decreasing the Ca8-P and Olsen-P fractions. This promoted adsorption of high-activity inorganic P to organic matter, consequently limiting the accumulation of Ca8-P. The long-term application of swine manure altered soil P fractions and enhances P lability, enhancing P lability and availability. This study identified an optimized the risk of leaching of labile P from soil under a fertilization regime applying 10,300 kg ha−1 yr−1 swine manure. To further mitigate the risk of P leaching, the inclusion of additional cellulose-based organic matter (e.g., straw) in swine manure fertilization regimes is recommended.

Oilseed rape-rice rotation with recommended fertilization and straw returning enhances soil organic carbon sequestration through influencing macroaggregates and molecular complexity

The sequestration of soil organic carbon (SOC) is influenced by agricultural management practices. However, the mechanisms that underlie the impacts of diverse paddy-upland rotation systems and fertilization strategies on SOC sequestration remain to be elucidated. Within this study, we investigated the variations in SOC sequestration under oilseed rape-rice (OR) and wheat-rice (WR) rotations with various fertilization regimes (CK, without fertilization; EF, excessive fertilization; RF, recommended fertilization; and RF+S, recommended fertilization with straw returning) through a five-year field experiment. Crop C inputs, soil aggregate-associated organic C, SOC functional groups, and their importance in SOC sequestration were examined. The results revealed that both SOC stock and sequestration rate were significantly enhanced by the OR rotation and straw returning. Specifically, SOC stock and sequestration rates were 7.7–14.3% and 28.6–83.7% higher in the OR rotation compared to the WR rotation under the same fertilization treatment. In addition, SOC stocks and sequestration rates were 6.7–13.3% and 24.4–77.6% higher in the RF+S treatment compared to the RF treatment. However, excessive fertilization (EF) did not further promote SOC sequestration. The OR rotation also showed higher values of aggregate mean weight diameter (MWD) and large-macroaggregate C compared to the WR rotation, with increases of 7.7–14.1% and 4.7–33.8%, respectively. The nuclear magnetic resonance studies showed that the OR rotation exhibited an elevated abundance of aromatic C and a more intricate molecular configuration compared to the WR rotation. Furthermore, crop C input increased significantly with fertilization, and crop C input was the highest in the RF+S treatment. The aggregate MWD and large-macroaggregate C also significantly increased after fertilization. Partial least squares path modeling revealed that crop rotation affected SOC sequestration mainly through its effect on large-macroaggregate C and SOC molecular structure complexity, whereas fertilization mainly affected SOC sequestration though its effects on crop C inputs and large-macroaggregate C. We conclude that OR rotation combined with straw returning is an effective measure for enhancing SOC sequestration in paddy-upland rotation systems, contributing to mitigating climate change in agricultural systems.

Assessment of CH4 flux and its influencing drivers in the rice-wheat agroecosystem of the Huai River Basin, China

To understand the CH4 flux variations and their climatic drivers in the rice-wheat agroecosystem in the Huai River Basin of China, the CH4 flux was observed by using open-path eddy covariance at a typical rice-wheat rotation system in Anhui Province from November 2019 to October 2021. The variations and their drivers were then analyzed with the Akaike information criterion method. CH4 flux showed distinct diurnal variations with single peaks during 9:00~13:00 local time. The highest peak was 2.15 µg m-2 s-1 which occurred at 11:00 in the vegetative growth stage in the rice growing season (RGS). CH4 flux also showed significant seasonal variations. The average CH4 flux in the vegetative growth stage in the RGS (193.8±74.2 mg m-2 d-1) was the highest among all growth stages. The annual total CH4 flux in the non-rice growing season (3.2 g m-2, 11.8%) was relatively small compared to that in the RGS (23.9 g m-2, 88.2%). CH4 flux increased significantly with increase in air temperature, soil temperature, and soil water content in both the RGS and the non-RGS, while it decreased significantly with increase in vapor pressure deficit in the RGS. This study provided a comprehensive understanding of the CH4 flux and its drivers in the rice-wheat rotation agroecosystem in the Huai River Basin of China. In addition, our findings will be helpful for the validation and adjustment of the CH4 models in this region.

Dry direct-seeded rice-wheat rotation system: Lower water and carbon footprint and higher carbon production efficiency and net ecosystem economic benefits

ContextSecuring food and energy supplies presents a significant challenge to human survival and progress. The rice-wheat rotation is one of the main food crop systems in China. However, no comprehensive assessment of the water footprint (WF), carbon footprint (CF), carbon production efficiency (CPE), and net ecosystem economic benefits (NEEB) of different rice-wheat rotation systems has been reported. ObjectiveThis study was conducted to assess the productivity, WF, CF, CPE, and NEEB of dry direct-seeded, wet direct-seeded, and transplanted rice-wheat (TR-W) rotation systems. The objective was to provide theoretical guidance for the development of clean and sustainable rice-wheat rotation production technology systems. MethodsWe used a split-plot design, with the main plots being the dry direct-seeded, wet direct-seeded, and transplanted rice planting methods, and the subplots consisting of two rice genotypes. Dry direct-seeded rice was mainly rainfed, ensuring that the soil remained relatively dry so that the soil is kept aerobic during the whole reproductive period of rice. Wet direct-seeded rice continues to keep the soil moist after sowing; the plots were maintained under a water layer of 3–10 cm following the emergence of 4.5 leaves, a condition that persisted until two weeks prior to the rice harvest. Transplanted rice was maintained under a water layer of 3–10 cm following transplanting until 2 weeks prior to the rice harvest. Subsequent to the rice harvest, the above-ground rice stubble was removed, and the ground was tilled with a rotary tiller in preparation for the subsequent wheat planting. ResultsResults demonstrated that the annual yield from the dry direct-seeded rice-wheat (DR-W) rotation system was comparable to the wet direct-seeded rice-wheat (WR-W) rotation system, while significantly lower than that of the TR-W rotation system. However, the WF of DR-W rotation system was 45.96% and 33.94% lower than that of WR-W and TR-W rotation systems, respectively, and the CF was 35.57% and 25.87% lower than that of WR-W and TR-W rotation systems, respectively. Therefore, its water and carbon production efficiencies were higher than those of WR-W and TR-W rotation systems. In addition, NEEB in DR-W and TR-W was comparable and significantly higher than that in WR-W. ConclusionsThe DR-W rotation was a promising rotation system according to the comparable annual yield, lower water and carbon footprints, higher CPE, and comparable or higher NEEB in comparison with other rice-wheat rotation systems. SignificanceWe suggest that the DR-W rotation should be promoted as the primary production mode for the rice-wheat rotation.

Microplastics alter soil structure and microbial community composition

Microplastics (MPs), including conventional hard-to-biodegrade petroleum-based and faster biodegradable plant-based ones, impact soil structure and microbiota in turn affecting the biodiversity and functions of terrestrial ecosystems. Herein, we investigated the effects of conventional and biodegradable MPs on aggregate distribution and microbial community composition in microhabitats at the aggregate scale. Two MP types (polyethylene (PE) and polylactic acid (PLA) with increasing size (50, 150, and 300 μm)) were mixed with a silty loam soil (0–20 cm) at a ratio of 0.5 % (w/w) in a rice–wheat rotation system in a greenhouse under 25 °C for one year. The effects on aggregation, bacterial communities and their co-occurrence networks were investigated as a function of MP aggregate size. Conventional and biodegradable MPs generally had similar effects on soil aggregation and bacterial communities. They increased the proportion of microaggregates from 17 % to 32 %, while reducing the macroaggregates from 84 % to 68 %. The aggregate stability decreased from 1.4 mm to 1.0–1.1 mm independently of MP size due to the decline in the binding agents gluing soil particles (e.g., microbial byproducts and proteinaceous substances). MP type and amount strongly affected the bacterial community structure, accounting for 54 % of the variance. Due to less bioavailable organics, bacterial community composition within microaggregates was more sensitive to MPs addition compared to macroaggregates. Co-occurrence network analysis revealed that MPs exacerbated competition among bacteria and increased the complexity of bacterial networks. Such effects were stronger for PE than PLA MPs due to the higher persistence of PE in soils. Proteobacteria, Bacteroidetes, Chloroflexi, Actinobacteria, and Gemmatimonadetes were the keystone taxa in macroaggregates, while Actinobacteria and Chloroflexi were the keystone taxa in microaggregates. Proteobacteria, Actinobacteria, and Chloroflexi were the most sensitive bacteria to MPs addition. Overall, both conventional and biodegradable MPs reduced the portion of large and stable aggregates, altering bacterial community structures and keystone taxa, and consequently, the functions.

Responses of Yield and Photosynthetic Characteristics of Rice to Climate Resources under Different Crop Rotation Patterns and Planting Methods.

Climate is the most important environmental factor influencing yield during rice growth and development. To investigate the relationships between climate and yield under different crop rotation patterns and planting methods, three typical rotation patterns (vegetable-rice (V), rape-rice (R), and wheat-rice (W)) and two mechanical planting methods (mechanical transplanting (T1) and mechanical direct seeding (T2)) were established. The results showed that compared to the V rotation pattern, the average daily temperature (ADT) during the sowing to heading stage increased under both R and W rotation patterns, which significantly shortened the growth period. Thus, the effective accumulated temperature (EAT), photosynthetic capacity, effective panicle (EP), and spikelet per panicle (SP) under R and W rotation patterns significantly decreased, leading to reductions in grain yield (GY). VT2 had a higher ratio of productive tillers (RPT), relative chlorophyll content (SPAD), leaf area index (LAI), and net photosynthetic rate (Pn) than those of VT1, which significantly increased panicle dry matter accumulation (DMA), resulting in an increase in GY. Although RT2 and WT2 had a higher RPT than those of RT1 and WT1, the GY of RT1 and WT1 decreased due to the significant reductions in EAT and photosynthetic capacity. Principal component analysis (PCA) showed that the comprehensive score for different rotation patterns followed the order of V > R > T with VT2 ranking first. The structural equation model (SEM) showed that EAT and ADT were the most important climate factors affecting yield, with total effects of 0.520 and -0.446, respectively. In conclusion, mechanical direct seeding under vegetable-rice rotation pattern and mechanical transplanting under rape-rice or wheat-rice rotation pattern were the rice-planting methods that optimized the climate resources in southwest China.

Hydrochar and Its Dissolved Organic Matter Aged in a 30-Month Rice-Wheat Rotation System: Do Primary Aging Factors Alter at Different Stages?

Hydrochar, recognized as a green and sustainable soil amendment, has garnered significant attention. However, information on the aging process in soil and the temporal variability of hydrochar remains limited. This study delves deeper into the interaction between hydrochar and soil, focusing on primary factors influencing hydrochar aging during a 30-month rice-wheat rotation system. The results showed that the initial aging of hydrochar (0-16 months) is accompanied by the development of specific surface area and leaching of hydrochar-derived dissolved organic matter (HDOM), resulting in a smaller particle size and reduced carbon content. The initial aging also features a mineral shield, while the later aging (16 to 30 months) involves surface oxidation. These processes collectively alter the surface charge, hydrophilicity, and composition of aged hydrochar. Furthermore, this study reveals a dynamic interaction between the HDOM and DOM derived from soil, plants, and microbes at different aging stages. Initially, there is a preference for decomposing labile carbon, whereas later stages involve the formation of components with higher aromaticity and molecular weight. These insights are crucial for understanding the soil aging effects on hydrochar and HDOM as well as evaluating the interfacial behavior of hydrochar as a sustainable soil amendment.

Effect of agricultural management practices on rice yield and greenhouse gas emissions in the rice–wheat rotation system in China

Agricultural management practices (AMPs) have the potential to significantly enhance crop yield, albeit with the possible side effect of escalating greenhouse gas emissions. Few studies have undertaken a comprehensive quantification of the impact of AMPs on crop production and soil GHG, particularly in identifying the optimal AMPs for rice cultivation within rice-wheat rotation system. Here, we combined data analysis and keyword search methods on 1433 individual experimental observations from 172 studies on diverse soil types in the subtropical monsoon climate zone of China to assess the impact of AMPs on rice yield, CH4 and N2O emissions, total greenhouse gas emissions (TGHGE). We focused on four key AMPs: mineral N fertilizer management (including ordinary N fertilizer and slow−/controlled-release fertilizer (SCRF)), organic material management (incorporating organic fertilizer, biochar amendment, and straw return), water-saving irrigation, and no-tillage. Our result showed the rice yield ranged from 2525 to 31,196 kg ha−1, and mineral N fertilizer and organic material management boosted rice yield by 2.84–16.19 % and 2.47–8.52 %, respectively. In terms of N2O emissions, biochar amendment resulted in a decrease of 13.05 %, while ordinary N fertilizer, organic fertilizer, and water-saving irrigation led to increases of 63.16 %, 136.66 %, and 37.41 %, respectively. The implementation of SCRF, water-saving irrigation, and no-tillage significantly curtailed CH4 (6.83 %–35.91 %) and TGHGE (6.22 %–20.59 %). Conversely, organic fertilizer and straw return significantly escalated CH4 emissions by 102.20 % and 33.64 % and TGHGE by 85.03 % and 32.40 %. Rice yield and GHG emissions are mainly influenced by variables such as soil bulk density, pH, soil organic carbon, soil texture, mean annual temperature, and total nitrogen. Our study demonstrates that the application of SCRF, water-saving irrigation, and no-tillage can effectively reduce GHG without compromising yield. These practices are particularly effective under climatic and soil conditions of rice-wheat rotation systems in China, thereby contributing to the sustainable rice farming.

Boosting Crop Growth Rates of Hybrid Rice (Pukhraj) through Synergistic Use of Organic Nitrogen Sources in Conjunction with Urea Nitrogen

In Northwestern Pakistan’s rice-based cropping systems, the prevalent reliance on inorganic nitrogen fertilizers (INF) has led to insufficient nitrogen (N) contributions from soil organic manures (OM). This study aims to evaluate the impact of organic sources (OS), including animal manures (AM) and crop residues (CR), on crop growth rates (CGR) in a rice-wheat rotation. A two-year field experiment involving hybrid rice (Oryza sativa L., Pukhraj) was conducted in Batkhela, Khyber Pakhtunkhwa. Various OS and inorganic-N (urea) combinations were applied, emphasizing their influence on CGR. The findings highlight poultry manure (PM) application as the most impactful on CGR, while wheat straw (WS) application resulted in the lowest CGR among the six OS investigated. Additionally, the use of AM showcased superior CGR compared to CR. In the initial year, the highest CGR occurred, with 75% of N sourced from urea and 25% from OS. In the second year, a balanced 50% N application from each source yielded the highest CGR. Urea and PM demonstrated the most robust CGR among OS combinations, while urea and WS yielded the lowest. Notably, onion leaves, a cost-effective option, delivered promising results comparable to berseem residues, indicating their potential as organic manure, especially in sulfur-deficient soils. These findings underscore the viability of onion residue management as a cost-effective alternative to ammonium sulfate fertilizers with global applicability. The abstract recommends promoting organic sources, particularly poultry manure and onion leaves, alongside inorganic-N fertilizers to enhance CGR and reduce dependence on costly alternatives. However, further research and field trials are necessary to explore the long-term impacts of these organic sources on soil health, nutrient cycling, and the sustainability of rice-based cropping systems in Northwestern Pakistan and beyond. In conclusion, this study investigates the influence of organic sources on CGR in rice-wheat rotations, emphasizing the superiority of poultry manure and onion leaves. The findings highlight cost-effective alternatives to conventional fertilizers, emphasizing the need for further research to validate long-term sustainability and applicability beyond the study area.

Straw return influences the structure and functioning of arbuscular mycorrhizal fungal community in a rice-wheat rotation system

Straw incorporation is a sustainable soil fertility-building practice, which can affect soil microbial communities. However, how straw incorporation affects arbuscular mycorrhizal fungi (AMF) is not well explored. Here, we studied the impacts of different straw management practices over eight years on the structure and function of AMF communities in a rice-wheat rotation system. The straw managements included no tillage with no straw (NTNS), rotary tillage straw return (RTSR) and ditch-buried straw return (DBSR). The community structure of AMF was characterized using high-throughput sequencing and the mycorrhizal functioning was quantified using an in situ mycorrhizal-suppression treatment. Different straw management practices formed unique AMF community structure, which was closely related to changes in soil total organic carbon, available phosphorus, total nitrogen, ammonium and nitrate. RTSR significantly increased Shannon diversity in 0-10 cm soils while DBSR increased it in 10–20 cm soils when compared with NTNS. DBSR significantly increased hyphal length density in the whole (0–20 cm) ploughing layer, but RTSR only increased it in the subsoil layer (10–20 cm). The mycorrhizal responses of shoot biomass and nutrient (N and P) uptake were positive under both straw incorporation treatments (RTSR and DBSR), but negative under NTNS. AMF community composition was significantly correlated to hyphal length density, and the latter was further a positive predictor for the mycorrhizal responses of plant growth and nutrient uptake. These findings suggest that straw return can affect AMF community structure and functioning, and farmers should manage mycorrhizas to strengthen their beneficial effects on crop production.

Effects of Straw Return Duration on Soil Carbon Fractions and Wheat Yield in Rice–Wheat Cropping System

In China’s subtropical rice–wheat cropping system, the changes in the soil organic carbon (SOC) pool due to long-term straw return and its connection with crop yield remain unclear. This study aims to provide insights into establishing a sensible straw return system by evaluating the differences in the distribution and variation rates of SOC, light fraction organic carbon (LFOC), heavy fraction organic carbon (HFOC), particulate organic carbon (POC), and mineral-associated organic carbon (MOC) in the 0–20 cm soil layer under different durations of straw return. Additionally, the study analyzes the relationship between the changes in SOC and its fractions and wheat yield. The experiment was conducted in 2019 in a rice–wheat rotation field with ten years of straw return treatments: no straw return (NR) or 1, 2, 3, 4, 5, 6, 7, 8, 9 year(s) of straw return (SR1–9), and an additional treatment in 2020 (10 years of straw return, SR10). The results revealed that with an increase in the duration of straw return, the contents of SOC, LFOC, HFOC, and POC gradually increased, showing the highest increments of 45.88%, 187.22%, 41.55%, 97.89%, and 28.21%, respectively, compared to the NR treatment. However, after eight years of straw return, the compound annual increase in soil organic carbon and its components was lower than in years 1–8, indicating a trend of diminishing increments. The SOC content and its variation were significantly correlated with the content and variation of LFOC, HFOC, POC, and MOC, with the highest sensitivity observed for the variation in LFOC, indicating the strong influence of the duration of straw return. The SOC and its fraction contents showed significant positive correlations with wheat yield, with the highest contribution to wheat yield increase attributed to an increase in LFOC content. In summary, straw return enhances the 0–20 cm deep soil carbon pool, with LFOC being the most sensitive indicator, reflecting the influence of the duration of straw return on soil carbon pools.

Environmental Impact Assessment of Rice–Wheat Rotation Considering Annual Nitrogen Application Rate

Rice–wheat rotation is a widely adopted multiple-cropping system in the Yangtze River Basin, China. Nitrogen (N) fertilizer is a key factor in regulating crop yield; however, only a few studies have considered the impact of annual N application on the yield, environmental impacts, and economic profits of rice–wheat rotation systems. In this study, a field experiment was conducted in the Jiangsu Province from 2020 to 2022. The rice and wheat seasons included six and five N fertilizer application rates, respectively (Rice: 0, 180, 240, 300, 360, and 420 kg N ha−1; Wheat: 0, 180, 240, 300, and 360 kg N ha−1), combined to form a total of 30 treatments. Life-cycle assessment was used to evaluate the environmental impacts of rice–wheat rotation under different N application treatments, using area, yield, and economic profit as functional units. Ten environmental impact categories were selected, including global warming. The results showed that grain yield did not consistently increase with an increase in N application, and the annual yield was the highest when 300 and 240 kg N ha−1 (R300W240 treatment) was applied in the rice and wheat seasons, respectively. The area-based weighting index of the R300W240 treatment ranked 20th among the 30 treatments, while the yield- and profit-based weighting indices were the lowest among the 30 treatments, decreasing by 14.9% and 28.7%, respectively, compared to the other treatments. The R300W240 treatment was the optimal annual N application strategy for rice–wheat rotation. Among the 10 environmental impacts considered, urea production contributed significantly to over eight environmental impacts, whereas the pollutant losses caused by its application contributed significantly to six environmental impacts. These findings reveal the dependence of the rice–wheat rotation system on the unsustainable use of N fertilizer and indicate that N fertilizer management practices should be further optimized to improve the environmental sustainability of grain production in the future.

Optimizing planting density and nitrogen application to mitigate yield loss and improve grain quality of late-sown wheat under rice-wheat rotation

Late sowing is a critical factor that hinders achieving high-yield, good-quality wheat under rice-wheat rotation. Understanding the physiological basis and regulatory pathways that lead to late-sown wheat of high yield and good quality is crucial for developing effective cultivation strategies. A 2-year field experiment was conducted to investigate the effects of sowing date, nitrogen (N) application rate, and planting density on wheat yield, grain quality, population characteristics, and the underlying physiological factors. The results revealed significant interactions among the sowing date, planting density, and N application in regulating both yield and quality. Late sowing reduced grain yield primarily by reducing the number of spikes and kernels. However, the latter was improved by increasing N application and the planting density, thus mitigating the yield losses caused by late sowing. Moreover, the grain protein content (GPC) and wet gluten content (WGC) increased with delayed sowing dates and higher N rates, but decreased with increased planting densities. For wheat yields over 9,000 or 7,500 kg ha–1, the latest sowing date should not be later than Nov. 4 or 15, respectively. In addition, specific criteria should be met, including a maximum of 1.5 and 1.0 million stems and tillers ha–1, a maximum leaf area index of 6.7 and 5.5, and a dry matter accumulation (DMA) at anthesis of 14,000 and 12,000 kg ha–1, respectively. For high-yield, good-quality late-sown wheat, the optimal combination is a 25% increase in the N rate (300 kg N ha–1) and a planting density of 2.25 million (N300D225) or 3.75 million (N300D375) plants ha–1 for 10- or 20-day delays in sowing, respectively. These combinations result in higher leaf net photosynthetic rate; higher activities of leaf nitrate reductase, glutamine synthetase, and grain glutamic pyruvic transaminase; and a lower sugar-N ratio during post-anthesis.

Optimal N management affects the fate of urea-15N and improves N uptake and utilization of wheat in different rotation systems.

Rice-wheat and maize-wheat rotations are major cropping systems in the middle and lower reaches of Yangtze River in China, where high nitrogen (N) inputs and low N efficiency often exacerbate resource waste and environmental pollution. Due to the changes in factors such as soil properties and moisture content, the N fate and the N utilization characteristics of wheat in different rotations are significantly different. Efficient N management strategies are thus urgently required for promoting maximum wheat yield in different rotation systems while reducing N loss. A 2-year field experiment using isotopic (15N) tracer technique was conducted to evaluate the fate of 15N-labeled urea in wheat fields and the distribution characteristics of N derived from different sources. The wheat yield and N use efficiency under various N rates (180 and 240 kg ha-1, abbreviated as N180 and N240) and preceding crops (rice and maize, abbreviated as R-wheat and M-wheat) were also investigated. The results showed that N240 increased N uptake and grain yield by only 8.77-14.97% and 2.51-4.49% compared with N 180, but decreased N agronomic efficiency (NAE) and N physiological efficiency (NPE) by 14.78-18.79% and 14.06-31.35%. N240 also decreased N recovery in plants by 2.8% on average compared with N180, and increased N residue in soil and N loss to the environment. Compared with that of basal N, the higher proportion of topdressing N was absorbed by wheat rather than lost to the environment. In addition, the accumulation of topdressing N in grain was much higher than that of basal N. Compared with that in R-wheat treatment, plants in M-wheat treatment trended to absorb more 15N and reduce unaccounted N loss, resulting in higher yield potential. Moreover, the M-wheat treatment increased N recovery in 0-20 cm soil but decreased 80-100 cm soil compared with R-wheat treatment, indicating a lower risk of N loss in deeper soil. Collectively, reducing N application rate and increasing the topdressing ratio is an effective way to balance sustainable crop yield for a secure food supply and environmental benefit, which is more urgent in rice-wheat rotation.