Straw return influences the structure and functioning of arbuscular mycorrhizal fungal community in a rice-wheat rotation system

Abstract

Straw incorporation is a sustainable soil fertility-building practice, which can affect soil microbial communities. However, how straw incorporation affects arbuscular mycorrhizal fungi (AMF) is not well explored. Here, we studied the impacts of different straw management practices over eight years on the structure and function of AMF communities in a rice-wheat rotation system. The straw managements included no tillage with no straw (NTNS), rotary tillage straw return (RTSR) and ditch-buried straw return (DBSR). The community structure of AMF was characterized using high-throughput sequencing and the mycorrhizal functioning was quantified using an in situ mycorrhizal-suppression treatment. Different straw management practices formed unique AMF community structure, which was closely related to changes in soil total organic carbon, available phosphorus, total nitrogen, ammonium and nitrate. RTSR significantly increased Shannon diversity in 0-10 cm soils while DBSR increased it in 10–20 cm soils when compared with NTNS. DBSR significantly increased hyphal length density in the whole (0–20 cm) ploughing layer, but RTSR only increased it in the subsoil layer (10–20 cm). The mycorrhizal responses of shoot biomass and nutrient (N and P) uptake were positive under both straw incorporation treatments (RTSR and DBSR), but negative under NTNS. AMF community composition was significantly correlated to hyphal length density, and the latter was further a positive predictor for the mycorrhizal responses of plant growth and nutrient uptake. These findings suggest that straw return can affect AMF community structure and functioning, and farmers should manage mycorrhizas to strengthen their beneficial effects on crop production.