Simple SummaryIn recent years, the soaring price of corn–soybean meal has led to an increase in the price of feed, which has brought significant challenges to the pig industry. The addition of more grains to pig diets will help develop new feed resources and improve profits. In this study, we focused on the effects of yeast culture (YC) supplementation on growth performance, meat quality, and gut microbiota of growing–finishing pigs. Three experimental diets were compared: a corn–soybean-based diet (CON), a wheat–rice-based diet (GRA), and a wheat–rice-based diet supplemented with YC. The growth performance of pigs on the wheat–rice-based diet was similar to that of pigs on the corn–soybean-based diet, but the feed cost reduced by 6.7% compared with CON. The dressing percentages of pigs in GRA and YC also increased by 1.43% and 2.15%, respectively. Meat color and antioxidant capacity improved after YC supplementation. YC supplementation promotes the proliferation of probiotics, inhibits the growth of harmful bacteria, and improves intestinal health. Application of a cereal-based diet and YC supplementation in growing pigs appears to be a very promising strategy for cost saving and economic benefits for the swine industry. The aim of this study was to investigate the effects of yeast culture (Saccharomyces cerevisiae) supplementation on the growth performance, meat quality, gut health, and microbiota community of growing–finishing pigs. A total of 45 growing–finishing pigs were randomly allocated to three treatments: a corn–soybean-based diet (CON, n = 15), a wheat–rice-based diet (GRA, n = 15), and GRA supplemented with 500 mg/kg yeast culture (YC, n = 15). The results show that compared to the CON group, the GRA group exhibited no significant differences in feed intake, daily gain, or feed conversion ratio, but had significantly reduced feed cost per kilogram BW gain of the finishing pigs (p < 0.05). Compared to that of the CON group, the GRA and YC groups showed an increase in the dressing percentage (p < 0.1). The meat color redness of the YC group increased (p < 0.1), whereas the b* value at 24 h decreased (p < 0.1). Meanwhile, the addition of YC significantly increased total superoxide dismutase activity on day 30 and catalase activity on day 60 (p < 0.05), and decreased serum urea nitrogen content on day 60 (p < 0.05). Furthermore, YC supplementation increased the gene expression of the duodenal anti-inflammatory factor IL-10 (p < 0.05), while it significantly decreased the gene expression of the ileal pro-inflammatory factor IL-8 (p < 0.05). The intestinal microbial identification results show that compared to the CON group, the YC group showed an increase in the relative abundances of Lactobacillus, Streptococcus, and Clostridium in the colon, and a decrease in the relative abundances of Bacteroidea, Clostridae, and Prevotella in the cecum. In conclusion, the growth performance of pigs on a wheat–rice-based diet was similar to that of pigs on a corn–soybean-based diet. Supplementation of 0.5% YC in the wheat–rice-based diet could improve the dressing percentage and meat color of growing–finishing pigs, which might be due to the increase in nitrogen utility and antioxidant capacity, and the improvement of the immune system and changes in microbiota communities.