Acorus Calamus Rhizome Research Articles

Impact of season on phytochemistry with special reference to β-Asarone content, extract yield and Pharmacognostic parameter in rhizome of Acorus calamus L.

Demand of medicinal plants is growing exponentially due to its extensive use in nutraceutical, cosmeceutical and pharmaceutical industries. The major problem is the variation in quality and quantity of phytoconstituents with the collection of medicinal plants in each batch. Though, the quality and quantity of phytoconstituents in plants are influenced by various factors, chief among them is seasonal variation. Rhizome of Acorus calamus L. is used in treatment of neurological disorder specially in children. The Ayurvedic system of medicine believes blends of constituent imparts therapeutic activities. The main chemical constituent responsible for cognition enhancement is Asarone. An attempt has been taken to record change in Pharmacognostic parameter with season and evaluate the best collection time of rhizome of Acaorus calamus L. with special reference to extractive value and asarone content.

Determination of the amount of flavonoids in the rhizomes of Acorus calamus

Determination of the amount of flavonoids in the rhizomes of Acorus calamus

Comparative Analysis of Dry Market Sample of Acorus calamus Linn. Sample with Pharmacopoeial Standards

A comparative analysis of market-available dry Acorus calamus Rhizome samples with pharmacopoeial standards provides valuable insights into the quality and authenticity of these products. The study revealed significant variations in morphological features among the market samples, highlighting the need for stringent quality control measures. The sample was identified as Acorus calamus Linn. While the drug meets API standards, it lacks a few features. Most of the samples are of irregular shape rather than cylindrical, and triangular, transverse leaf scars were absent. These observations suggest potential variations or discrepancies in the market-available samples compared to pharmacopoeial standards. Further investigations and quality control measures are essential to ensure the consistency and efficacy of Acorus calamus products in traditional and modern medicinal practices.

Effect of pH, Amount of Metal Precursor, and Reduction Time on The Optical Properties and Size of Zinc Oxide Nanoparticles Synthesized Using Aqueous Extract of Rhizomes of Acorus calamus

Nanoparticles possess various unique characteristics compared to the corresponding bulk materials. Large band gap, non-toxic nature, and multi-applicability are the worthwhile characteristics of zinc oxide to be synthesized and studied. The size of nanoparticles can be controlled by varying the different experimental conditions. This paper reports the synthesis of zinc oxide nanoparticles by using an aqueous extract of rhizomes of Acorus calamus, where the bio-components present in aqueous extract acted as reducing agents. The size and band gap energy of zinc oxide nanoparticles were studied by varying different parameters such as pH, concentration of the metal precursor, and reduction time. The variations in the size of nanoparticles were studied by UV-visible spectroscopy. FTIR showed phenolic compounds, primary amines, and amides (proteins/enzymes) as the functional groups responsible for the reduction of metal precursors to form nanoparticles. The surface morphology of nanoparticles was studied by FE-SEM image. The FE-SEM image displayed the formation of various shapes and agglomeration of the nanoparticles. XRD pattern revealed that the average size of zinc nanoparticles is 10 nm. In vitro antibacterial activity of ZnO nanoparticles has been assayed against gram-positive and gram-negative bacteria. The growth inhibitory activity of nanoparticles against different bacterial pathogens has also been determined

VALIDATION EVALUATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF α(1,2)-L-RHAMNO-α(1,4)-D-GALACTOPYRANOSYLURONANE ACORUS CALAMUS L. IN DOSAGE FORM

Introduction. The object of increased attention of researchers is calamus (Acorus calamus L.), for the components of which a wide range of pharma-cological activities have been identified – immunomodulatory, anti-inflammatory, analgesic, antioxidant, neuroprotective, hypolipidemic, antitumor. Ac-cording to previous studies, it was found that α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronan, isolated from the rhizomes of Acorus calamus L., has antimetastatic, antiblastoma, and immunomodulatory properties. The substance can also reduce the toxic effect on the hematopoietic system and in-crease the effectiveness of chemotherapy. Based on it, a finished dosage form was obtained - a solution for intravenous administration of 10 mg/ml, which requires the development of valid quality control methods for inclusion in the draft regulatory document on the quality and registration of a new drug. The aim of the study was to determine the metrological characteristics of the chromatographic technique for the quantitative determination of α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronan in the finished dosage form. Material and methods. The object of the study was the finished dosage form - a solution for intravenous administration of α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronan Acorus calamus L. 10 mg/ml, produced on the basis of the educational establishment of the Technology Implementation Cen-ter of the Federal State Budgetary Educational Institution of Higher Education of the Siberian State Medical University of the Ministry of Health Russia. Quantitative determination was carried out using a Dionex Ultimate 3000 liquid chromatograph (Thermo, Germany) with a refractometric detector RI-101. Validation assessment was carried out according to the following indicators: linearity, accuracy, precision under repeatability conditions. Results. Linear regression analysis of the obtained results using the least squares method showed that the technique is linear in the concentration range of 80–120%. When determining the accuracy, the openability for concentrations of 80, 100 and 120% varied in the range of 99.68–101.96%. The values of the relative standard deviation did not exceed the permissible values when assessing precision 0.82–4.51%. Conclusions. The methodology is valid for the studied indicators and can be recommended for inclusion in the draft regulatory document on quality. The work was carried out within the framework of applied scientific research 720000F.99.1.BN62AB30000 within the framework of state assignment No. 056-00116-23-01.

Chemical composition and biological efficacy of Acorus calamus (L.) rhizome essential oil on Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Oryzaephilus surinamensis (L.) as stored-grain protectants

Key depredating agents affecting grains are insects which lead to considerable losses across post-harvest food supply chain. As their control strategies, plant-derived essential oils have become optimal competitors to synthetic insecticides. In view of this background, chemical composition, insecticidal activity in comparison with ultrastructural impact on cuticular topography, repellency and feeding deterrence of Acorus calamus (L.) rhizome essential oil were investigated towards Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Oryzaephilus surinamensis (L.). Chemical profile of oil was examined by gas chromatography-mass spectrometry (GC-MS). Morphological alterations on insects' cuticles triggered by oil's direct exposure were analyzed by scanning electron microscopy (SEM). α-Asarone (89.05%) was the major constituent of oil. Insecticidal bioassays showed that O. surinamensis (LC50 = 1.92 μl/g) was more susceptible than R. dominica (LC50 = 4.78 μl/g) and S. oryzae (LC50 = 9.61 μl/g) upon 24 h of exposure. Degree of insects' repellence was arranged in the declining order of O. surinamensis (RC50 = 0.96 μl/cm2) > S. oryzae (RC50 = 1.02 μl/cm2) > R. dominica (RC50 = 1.56 μl/cm2) after 1 h, wherein estimated values increased after 5 h of exposure. According to flour-disc bioassays, oil had the weakest feeding deterrence on O. surinamensis (AC50 = 4.37 mg/disc) leading to post-ingestive lethality induced by phagostimulation, than against S. oryzae (AC50 = 0.88 mg/disc) and R. dominica (AC50 = 1.80 mg/disc). SEM observations reflected severe impact on insects' cuticular topography suggesting that oil molecules might be entered into their bodies via cuticular penetration and oral ingestion influencing contact/post-ingestive toxicity. Hence, results demonstrate the potentiality of A. calamus essential oil for sustainable insect pest management in stored-grain ecosystems.

CHARACTERIZATION OF FRACTION CONTAINING BETA ASARONE ISOLATED FROM ACORUS CALAMUS LINN. RHI- ZOMES AND STUDYING THE AMELIORATIVE EFFECT OF JUGLANS REGIA KERNEL OIL ON ITS ANTIEPILEPTIC PO- TENTIAL

Sweet flag (Acorus calamus) has a long history of use in the treatment of a range of diseases, including inflammatory response, chest pain, digestive problems, and neurological disorders. The prime objective of the study is to isolate newer moieties from Acorus calamus (AC) rhizomes and to test their anticonvulsant potential with Juglans Regia Kernel Oil (Walnut Oil). Pharmacognostic standardization of AC rhizomes have been performed as per WHO guidelines of standardization. Methanolic extract of AC rhizomes was used for isolation of active moieties using column chromatography. Further, characterization of isolated moieties has been performed using UV, IR, 1H-NMR, C13- NMR and LCMS data. OECD 423 guidelines have been fol- lowed for the determination of oral acute toxicity. Swiss albino mice(n=6) were used for in-vivo studies. An- ticonvulsant study of isolated and characterized fraction of Beta Asarone was performed using scPTZ in- duced convulsion model. Pharmacognostic standardization of rhizomes showed results that fall within the pharmacopoeial limits. Spectral details of the fraction confirmed the identity as Beta Asarone. Juglans Regia Kernel oil and AC isolated fraction has shown a better ameliorative antiepileptic effect with prolonged time for seizure onset. Percentage protection has also been enhanced as compared to standard drug.

ISOLATION, CHEMICAL AND SPATIAL CHARACTERIZATION OF ACIDIC POLYSACCHARIDES FROM SOME PLANTS OF SIBERIAN FLORA WITH IMMUNOTROPIC ACTIVITY

Plant polysaccharides have a wide range of biological activity, including an immunotropic effect, the degree and magnitude of which depends on the composition and tertiary structure of the macromolecule. Acidic polysaccharides (APS) were isolated by extraction with an acidic aqueous solution (pH=3) and characterized From the rhizomes of Acorus calamus L., the aerial part of Centaurea pseudomaculosa Dobrocz., the herb Conium maculatum L., and the leaves of Betula pendula Roth. The content of total carbohydrates was determined for all samples (>90%), uronic acids (24.56–53.70%) and a small amount of impurity substances (protein and nucleic acids 0.81–1.02% and 0.016–0.035%, respectively); molecular weight distribution (Mn, Mw). Structural features were studied using physicochemical and instrumental methods, such as UV and IR spectroscopy, reaction with Congo red, and scanning electron microscopy. The polysaccharides of Acorus calamus, Conium maculatum, and Betula pendula have a triple-helical conformation and bulky ultrastructure; the polysaccharides of Centaurea pseudomaculosa are characterized by the absence of a triple-helical conformation and an amorphous ultrastructure.

Medicinal Common Seed-Acorus Calamus

This article covers the structure, distribution, chemical composition, and use of the common sedum plant in folk medicine and traditional medicine. It highlights the relevance of using this plant in the field of medicine. Acorus calamus is a perennial plant belonging to the Aceraceae family and is found in many countries. It contains various substances, including vitamin C. The plant is highly regarded in Indian traditional medicine and is known for rejuvenating the brain and nervous system. The rhizomes of Acorus calamus are widely used in the treatment of many diseases such as epilepsy, mental disorders, fever, abdominal pain, chronic diarrhea, liver, and kidney diseases.

Comparative chemical composition and pesticidal evaluation of Acorus calamus accessions collected from different geographical locations

The objectives of the present study were to investigate the phytochemical composition of essential oils (EO) from rhizomes of Acorus calamus collected from Jorhat, Assam; Munsyari and Pantnagar, Uttarakhand, India. EOs were studied for different pesticidal activities viz; nematicidal, insecticidal, and herbicidal activity. To study the synergistic effect of EOs on pesticidal activity, four combinations of EOs were prepared. Phenylpropanoids with β-asarone as the main compound were identified in all collections with varying percentages. Its contribution was found to be 85.8% in Munsyari EOs followed by 74.3% in Pantnagar and 62.6% in Assam collections. All EOs exhibited dose-dependent in vitro nematicidal activity against Meloidogyne incognita in terms of immobility and inhibition of egg hatching. The activity was observed as maximum in the EO combination of all three collections (1:1:1) whereas minimum in the Assam collections. In insecticidal activity against Lipaphis erysimi and Selepa celtis, maximum mortality was observed in Munsyari collections. The oils were assessed for sprout inhibition activity in terms of seed germination inhibition, coleoptile growth of the shoot and root against Raphanus raphanistrum. Maximum seed germination inhibition, % shoot, and root growth inhibition were found in all collections EO combinations. To predict the possible mode of action and the structure-activity relationship between major compounds of EOs and biological activities, in silico molecular docking and ADME/Tox studies were performed. The docking results revealed the mode of action of proteins of insects, nematodes, and weeds and were found in support of in vitro experiments. The study may be helpful for the development of herbal-based pesticides after proper clinical trials.

Formulasi dan Evaluasi Emulgel Ekstrak Rimpang Jeringau (Acorus calamus L.) sebagai anti-acne

Dermatology studies in Indonesia show an increase in acne vulgaris incidents with the highest prevalence in adolescents. Acne topical therapy generally contains retinoids. However, this therapeutic option has drawbacks, namely burning sensation, dry skin, erythema, and itching of the skin. Along with people's interest in using natural products, alternative therapies with natural products are increasingly having economic value. One of the natural ingredients that have the potential to be anti-acne is Acorus calamus L. Sweet pea (Acorus calamus L.) is a plant whose leaves and rhizomes are used empirically by the Dayak tribe in Borneo to treat skin diseases. Acorus calamus rhizome ethanol extract can inhibit the growth of Propionibacterium acnes bacteria that predominate in acne inflammation. Acorus calamus rhizome extract in this study was formulated in the form of an emulgel because it effectively delivers hydrophobic active substances. Emulgel is also not greasy but moisturizes the skin. The research aimed to produce an anti-acne emulgel formulation from Acorus calamus extract as a natural raw material that meets the physical stability requirements. The research was conducted at the Phytochemical Laboratory, Pharmaceutical Technology Laboratory, and Microbiology Laboratory of Samarinda School of Health Sciences. The research stages are the preparation of plant extract, phytochemical analysis, preparation of emulgel, evaluation of emulgel, and antibacterial activity assay. The Emulgel formulation was carried out with variations in the concentration of the gelling agent, namely Carbomer 940 0.5% (Formula A), 0.75% (Formula B0, and 1% (Formula C). The results of the physical evaluation showed all formulas met the requirements of the organoleptic test, homogeneity test, pH test, spreadability test, and viscosity test. All formulas have non-Newtonian flow types with plastic properties. The antibacterial test results show that all formulas can inhibit the growth of Propionibacterium acnes bacteria.

Efficacy of Plant Derivatives in Protecting Mungbean Grains against Cowpea Weevil (Callosobruchus maculatus) under Storage Conditions in Southern Bhutan

Food grains infestation by insect pests in stores is a severe challenge in food production around the globe, especially in wet and humid regions. In Bhutan, mung bean is commonly grown for consumption as a superior source of protein. However, severe cowpea weevil infestation is observed while in storage condition. Controlling with synthetic pesticides is associated with health risks due to toxic residues which intervene in safe and healthy protection methods. Protecting the grains in-store through organic approach is imperative for consumption and seed purposes. Some botanical plant extracts are known for their protective properties which need location-specific studies based on availability and suitability. Therefore, this study was conducted to evaluate the efficacy of eight treatments (Acorus calamus rhizome powder, mustard oil, garlic cloves, turmeric rhizome powder, wood ash, Vitex negundo leaf powder, super grain bag including untreated control) against cowpea weevil (Callosobruchus maculatus) in mungbean under storage condition. The experiment was laid in a randomized complete block design with three replications. The result revealed that the lowest mean number of grains perforated and percent grain perforated were recorded in grains treated with Acorus calamus rhizome powder (0.46 & 0.20 %) followed by mustard oil (1.05 & 0.47 %) and grains stored in super grain bag (5.74 & 2.49%), which were significantly lower (P<0.001) as compared to other treatments. The lowest number of adult cowpea weevils was also recorded in grains treated with Acorus calamus and mustard oil followed by grains stored in super grain bag. Germination percentage was found highest in wood ash followed by mustard oil, Acorus calamus and garlic-treated grains while the lowest was in super grain bag. Therefore, Acorus calamus rhizome powder and mustard oil were found to be effective in managing cowpea weevil without affecting seed germination and vigour.

Phytochemical analysis and evaluation of haemolytic potential of methanolic extracts of root of Chrysopogon zizanioides and rhizome of Acorus calamus

Many plants and plant-derived products have been used in the preparation of traditional medicine to prevent and treat various diseases. Even though medicinal plants are rich sources of bioactive compounds, analysis of their composition is a prerequisite along with an evaluation of their cytotoxicity. The haemolytic test determines the ability of any compound to destroy red blood cells, leading to the release of haemoglobin. Plants Chrysopogon zizanioides and Acorus calamus have been reported to exhibit several medicinal properties. Hence this study was designed to elucidate the medicinal plant extracts by phytochemical analysis and to evaluate the haemolytic potential of methanolic extracts of the root of Chrysopogon zizanioides and rhizome of Acorus calamus, towards the human erythrocytes, using spectrophotometer. Phytochemical analysis revealed the presence of alkaloids, tannins, terpenoids, steroids and carbohydrates in the crude extract of both plants. From the results of this study, it is clear that the percentage of haemolysis increases with an increase in the concentration of crude extract of the root of Chrysopogon zizanioides and rhizome of Acorus calamus treatments. However, in both the treated groups, the haemolysis percentage was very less; indicating that these extracts are safe for human erythrocytes.

The role of centrifugal partition chromatography in the removal of β-asarone from Acorus calamus essential oil

Β-asarone is a phenylpropane derivative present in the rhizomes of Acorus calamus, that was proved to exhibit toxic effects in humans. Because of its presence the whole plant that is commonly used in traditional medicine for its sedative, anti-inflammatory, neuroprotective and other properties has limited application nowadays. In the study, qualitative and quantitative analysis of a collection of nine essential oil (EO) samples of European and Asian origin was performed. The final content of β-asarone in the tested samples ranged between 0.265 and 1.885 mg/mL. Having in mind a possible application of the EO as a biopesticide, this research aimed at the development of CPC-based purification protocol that could help remove β-asarone from EO. It was proved that the biphasic solvent system composed of n-hexane/EtOAc/MeOH/water, 9:1:9:1 (v/v/v/v) was capable of the removal of the toxic constituent in the CPC chromatograph operated in the ascending elution mode with 2200 rpm and a flow rate of 5 mL/min. The chromatographic analysis that lasted only 144 min effectively separated β-asarone (purity of 95.5%) and α-asarone (purity of 93.7%) directly from the crude Acorus calamus rhizome EO.

Influence of thermal treatment on extraction and characteristics of phytochemicals from rhizome of Acorus calamus L

Influence of thermal treatment on extraction and characteristics of phytochemicals from rhizome of Acorus calamus L

Four pairs of tautomers from rhizomes of Acorus calamus and fruits of Cornus officinalis

A pair of new guaiane-type sesquiterpene tautomers (1) was isolated from rhizomes of Acorus calamus. Meanwhile, three pairs of known compounds, including a pair of dihydroflavone glycoside tautomers (2), a pair of 4-hydroxybenzoic acid ester glycoside tautomers (3), as well as a pair of secoiridoid glycoside tautomers (4) were isolated from fruits of Cornus officinalis. Their structures were elucidated by extensive spectroscopic and computational methods. Furthermore, the tautomeric mechanisms were discussed.

Synthesis and characterization of cry protein coated zinc oxide nanocomposites and its assessment against bacterial biofilm and mosquito vectors

Mosquitoes need to be eradicated as they can spread deadly diseases. Cry toxic proteins from Bacillus and zinc oxide nanoparticles also can tremendously control pest and bacterial pathogens. With this reference, the Ac-ZnO NPs was effectively synthesized using Acorus calamus rhizomes extract where after incorporated with bacterial cry toxic protein (Btp) to produce Btp-Ac-ZnO nanocomposites. The XRD and FTIR, disclose the crystalline form with an average size of 17.47 nm and the possible biomolecules of Btp-Ac-ZnO NCs. SEM and TEM make known the well agglomerated and cone shape of Btp-Ac-ZnO NCs. The NCs show concentration-dependent antioxidant activity. Btp-Ac-ZnO NCs drastically arrest the formation of biofilm by the pathogenic bacteria such as E. faecalis, S. aureus, P. aeruginosa, and P. vulgaris at 100 μg/mL. All the above, the Btp-Ac-ZnO NCs exhibits superior larvicidal activity against three mosquito vectors namely Ae. aegypti, An. stephensi and Cx. quinquefasciatus with LC50 values of 43.76, 39.60 and 37.13 μg/mL respectively. Besides, the biological enzymes are significantly reduced in the treated larvae than that of untreated one, which indicates the effect of Btp-Ac-ZnO NCs. Since, the Btp-Ac-ZnO NCs could be utilized against the pathogenic bacteria, and its biofilm structure, and also in the vector control sectors.

Molecular Mechanisms and Therapeutic Potential of α- and β-Asarone in the Treatment of Neurological Disorders.

Neurological disorders are important causes of morbidity and mortality around the world. The increasing prevalence of neurological disorders, associated with an aging population, has intensified the societal burden associated with these diseases, for which no effective treatment strategies currently exist. Therefore, the identification and development of novel therapeutic approaches, able to halt or reverse neuronal loss by targeting the underlying causal factors that lead to neurodegeneration and neuronal cell death, are urgently necessary. Plants and other natural products have been explored as sources of safe, naturally occurring secondary metabolites with potential neuroprotective properties. The secondary metabolites α- and β-asarone can be found in high levels in the rhizomes of the medicinal plant Acorus calamus (L.). α- and β-asarone exhibit multiple pharmacological properties including antioxidant, anti-inflammatory, antiapoptotic, anticancer, and neuroprotective effects. This paper aims to provide an overview of the current research on the therapeutic potential of α- and β-asarone in the treatment of neurological disorders, particularly neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), as well as cerebral ischemic disease, and epilepsy. Current research indicates that α- and β-asarone exert neuroprotective effects by mitigating oxidative stress, abnormal protein accumulation, neuroinflammation, neurotrophic factor deficit, and promoting neuronal cell survival, as well as activating various neuroprotective signalling pathways. Although the beneficial effects exerted by α- and β-asarone have been demonstrated through in vitro and in vivo animal studies, additional research is required to translate laboratory results into safe and effective therapies for patients with AD, PD, and other neurological and neurodegenerative diseases.

Effect of botanicals on number of egg laid by Callosobruchus chinensis in stored chickpea

An experiment was conducted to test the efficacy of botanicals against pulse beetle in stored chickpea. Eight treatment including untreated control comprising of Cinnamon powder (3 g/kg), Clove powder(3 g/kg), Black pepper seed powder(3 g/kg), Turmeric rhizome powder(5 g/kg), Acorus calamus rhizome (Vekhand) powder (2 g/kg), Acorus calamus rhizome (Vekhand) powder(4 g/kg), Acorus calamus rhizome (Vekhand) powder (8 g/kg) were used against adult pulse beetle, Callosobruchus chinensis on chickpea grains. All botanicals recorded minimum eggs laid by the pulse beetles on the grain treated with than the untreated control. Acorus calamus rhizome (Vekhand) powder (8 g/kg) and Acorus calamus rhizome (Vekhand) powder (4 g/kg) were found most effective in inhibiting egg laying even up to 6 month of storage after release of insect.

Evaluation of Essential Oil Extracted from Acorus calamus Rhizomes and Leaves

Abstract-Essential oils are volatile complex compounds which are characterized by a strong odour and are formed naturally by aromatic plants as secondary metabolites. The practical value of essential oils in daily life is explored through the study of numerous physicochemical characteristics (Parthiban et al., 2011). Physicochemical characteristics indirectly influence the quality of the essential oils. The commercial importance of oils mostly depends on these physicochemical characteristics, which provide baseline data to determine its suitability for consumption. Therefore, the present study was taken to evaluate characteristics of the essential oil extracted from the leaves and rhizomes of Acoruscalamus which is a wild aromatic plant species growing in the lower Himalayan region and the essential oil extracted from the plant is used frequently in skin care and aromatherapy to uplift mood and relieve stress. Essential oil was extracted from both rhizome and leaves (Ac R and Ac L) of Acoruscalamus and are analysed for characteristics like yield, specific gravity, viscosity, refractive index, acid number, saponification value, etc. Results indicated that that essential oil Ac R is more superior to Ac L in quality enhancing its commercial importance and can be taken up for further studies.