Abstract
Sweet flag (Acorus calamus) has a long history of use in the treatment of a range of diseases, including inflammatory response, chest pain, digestive problems, and neurological disorders. The prime objective of the study is to isolate newer moieties from Acorus calamus (AC) rhizomes and to test their anticonvulsant potential with Juglans Regia Kernel Oil (Walnut Oil). Pharmacognostic standardization of AC rhizomes have been performed as per WHO guidelines of standardization. Methanolic extract of AC rhizomes was used for isolation of active moieties using column chromatography. Further, characterization of isolated moieties has been performed using UV, IR, 1H-NMR, C13- NMR and LCMS data. OECD 423 guidelines have been fol- lowed for the determination of oral acute toxicity. Swiss albino mice(n=6) were used for in-vivo studies. An- ticonvulsant study of isolated and characterized fraction of Beta Asarone was performed using scPTZ in- duced convulsion model. Pharmacognostic standardization of rhizomes showed results that fall within the pharmacopoeial limits. Spectral details of the fraction confirmed the identity as Beta Asarone. Juglans Regia Kernel oil and AC isolated fraction has shown a better ameliorative antiepileptic effect with prolonged time for seizure onset. Percentage protection has also been enhanced as compared to standard drug.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
