Retention Of Bioactive Compounds Research Articles

Effect of Cryogenic grinding on chemical and morphological characteristics of mango (Mangifera indicaL.) peel powder

Mango peel waste (MPW) represents 15–20% of the overall mass of fruit. Hence, an attempt has been made to utilize MPW from six cultivars (Chausa, Langra, Neelum, Barah masi, Dashehari, and Fazli). The mango peel powder (MPP) was obtained using Cryo-Ball mill, which uses liquid nitrogen to achieve low temperature. The samples were subjected to proximate, mineral composition and SEM analysis. The MPP extracts were screened for their TPC, TFC, and IC50 values. The samples had considerable amount of protein (5.39–6.06%) and crude fiber (6.43–11.80%). Interestingly, significant amount of P and Cl was recorded with highest value in Barah masi (13.53%) and Chausa (2.89%), respectively, which are not reported earlier. Langra, Neelum, and Chausa were found to have highest values for TPC, TFC, and IC50 as 60.48 mg GAE/g, 135.04 mg QE/g, and 490.75 µg/mL, respectively. This study will provide the insight of relationship between Cryogenic grinding and retention of beneficial components. Practical applications The Cryogenic grinding (Cryo-grinding) is an emerging technology which is used for retention of beneficial components and results in end product with improved quality. This study will highlight the link between Cryo-grinding and retention of bioactive compounds. The chemical and mineral composition data obtained for Cryo-ground samples (mango peel powder) suggests their great potential to be used for the fortification of food products. In addition, it reduces problems related to waste disposal and improves functional quality of the food. The findings of this study will enlighten the researchers about incorporation of LN2 in grinding operation of heat-sensitive products.

Retention of Bioactive Compounds During Domestic Processing of Croatian Domestic Garlic (Allium sativum L.).

SUMMARYThe content of bioactive compounds and antioxidant activity were determined in Croatian domestic garlic after domestic processing (crushing, water blanching and frying) through different thermal treatments. The predominant phenolics in fresh garlic expressed per fresh mass were p-coumaric (10.79 mg/100 g) and caffeic (9.50 mg/100 g) acids, while the most abundant organosulfur compounds were methylsulfinylsulfanylmethane (9881.84 mg/100 g), 3-methylsulfinylsulfanylprop-1-ene and 3-methylsulfanylsulfinylprop-1-ene (257.59 mg/100 g) and allicin (185.62 mg/100 g). The highest total phenolic content and antioxidant activity were determined in fresh garlic followed by crushed, blanched and fried garlic, while organosulfur content increased after shorter thermal treatment. As time of treatment increased, frying showed the most pronounced losses of garlic total phenolic acids (in the range from 19.47 to 37.93%) and blanching of organosulfur content (about 25%). The blanching and frying significantly reduced allicin content, while S-methyl methanesulfinothioate was more stable.

Impact of ultrasound and blanching on functional properties of hot-air dried and freeze dried onions

The aim of this study was to investigate the effect of ultrasonic treatment and blanching prior to hot-air drying and freeze drying of onions on the retention of bioactive compounds (total phenolics, total flavonoids, and quercetin). Onion slices were treated either with ultrasound at 20 kHz and different amplitude levels (24.4–61 μm) for 1, 3 and 5 min or with blanching using hot water at 70 °C for 1, 3 and 5 min. The ultrasound treatment improved the retention of bioactive compounds (especially quercetin) and accordingly the antioxidant activity in onion slices dried either by freeze drying or hot-air drying. This is ascribed to the destruction of the original tissue structure by ultrasound and thus higher extraction ability of the studied phytochemicals. Comparing ultrasound treated samples, freeze dried onions had a higher retention of bioactive compounds than hot-air dried ones. Blanched and ultrasound treated dried onions exhibited similar colour change. Therefore, ultrasound treatment is a potential alternative to conventional blanching before drying of onion slices.

Effect of drying methods on physico-chemical properties and antioxidant activity of Dendrobium officinale

The present study aimed to investigate the effect of different drying methods, i.e., sun drying (SD), hot air drying (AD), vacuum drying (VD), infrared radiation drying (IR), and freeze drying (FD) on the physico-chemical properties of Dendrobium officinale. The results revealed that different drying methods showed significant differences in the color retention, water soluble polysaccharide content, total phenolic content, total flavonoid content, fatty acids, and antioxidant activities. FD, VD, IR and AD led to higher retention of bioactive compounds and better antioxidant activities, and FD showed the best. However, with respect to the water-soluble polysaccharides content, the characteristic components in D. officinale, AD was better than FD. The results showed that the quality of D. officinale depends on the drying method applied. Considering the retention of polysaccharide content and drying processing duration, AD is a promising treatment for D. officinale.

In-Vitro Antioxidant Capacity and Bioactive Compounds Preservation Post-Drying on Berrycacti (Myrtillocactus geometrizans)

Berrycactus is a cactus which does not require special agronomic attention, the berries are consumed locally and its commercialization is rather scarce because of the extremely short shelf-life. The significance of the application of any drying methods used to extend the shelf-life on the berrycacti is currently unknown. The aim of this work was to preserve berrycacti (Myrtillocactus geometrizans) and test the bioactive compounds and antioxidant capacity using two distinctive drying methods, freeze-drying (FD) and Instant Controlled Pressure Drop (DIC). Ripe berrycacti was chosen for the drying procedures because the antioxidant capacity and levels of soluble phenols and betalains were at their peak. Colour, phenols, non-extractable polyphenols, tannins, betalains, and antioxidant capacity were considered as factors to determine drying efficacy. Only colour parameters could discriminate between FD and DIC, concluding that both methods are suitable and efficient for preservation of antioxidant properties and retention of bioactive compounds. Both drying methods demonstrated higher in-vitro antioxidant capacity compared to the fresh fruit; highlighting the increase of non-extractable polyphenols and condensed tannins, and good retention of betalains and ascorbic acid after the drying treatments. This research points to use this sustainable crop to provide added value to berrycacti while considering this fruit as functional food due to the antioxidant capacity present even after being processed.

Standardization of storage conditions of marigold (Tagetes sp.) petal extract for retention of carotenoid pigments and their antioxidant activities

Present investigation was carried out to find the effect of different storage temperatures and durations on retentionof total carotenoids, antioxidant activities and other secondary metabolites of marigold petal extract in varieties Pusa Arpita, Pusa Basanti Gainda, and Pusa Narangi Gainda. It was observed that the highest retention of carotenoids, lutein, b-carotene, phenolic and flavonoid contents and antioxidant activities were recorded at storage temperature of -20°C followed by 4°C and lowest retention was observed at ambient storage temperature. It was revealed from the studies that the carotenoids, total phenolics, total flavonoids and antioxidant activities were decreased during storage. Among varieties, Pusa Narangi Gainda retained more carotenoids (1 925.26 mg/100g DW) and antioxidant activities (FRAP- 647.83 μ mol FeSO4/g; DPPH-59.51%), whereas, Pusa Arpita retained more lutein (228.87 μg/g) and β- carotene content (15.49μg/g) stored at -20°C. The storage of vacuum dried petals at -20°C temperatures was found suitable for higher retention of bioactive compounds.

Functional relationships between phytochemicals and drying conditions during the processing of blackcurrant pomace into powders

The effect of convective drying, microwave-vacuum drying and their combination applied to blackcurrant by-product on the quality being measured in terms of the content of phytochemicals in the powders was examined. Convective drying, along with the increase in the temperature, linearly reduced the content of anthocyanins, flavonols and chlorogenic acids. Microwave vacuum drying significantly shortened the drying time, and resulted in higher retention of bioactive compounds at higher temperatures in comparison to convection. No functional relationship was observed between bioactive compounds and the power of magnetrons thus between the processing temperature and the content of individual groups of polyphenols, apart from delphinidin-3-O-rutinoside and delphinidin-3-O-glucoside. During the dehydration of blackcurrant pomace the exponential formation of hydroxymethylfurfural was noticed. The antioxidant capacity decreased along with the increase in drying temperature and in parallel with the decrease in anthocyanins content, thus suggesting that those polyphenols considerably influence the antioxidant potential of final products.

The Effectiveness of Vacuum-microwave Drying Methods in the Preservation of Amelanchier Berries (Amelanchier canadensis L. Medik.)

Abstract In Poland, Amelanchier berries are of considerable interest to both consumers and potential producers, which presents marketing challenges and opportunities. An attempt was made to preserve Amelanchier berries using a vacuum-microwave method of drying in order to determine its usefulness for producing microbiologically stable dried fruit with a high nutritional values. The berry variety “Prince William” (Amelanchier canadensis) was chosen as the experimental material. Vacuum-microwave drying was performed in two variants: (convective drying [CD] + vacuum microwave method [VMW]) after convective pre-drying of fruit and (VMW) without pre-drying. Unassisted CD was used as the reference method. Application of a vacuum-microwave drying method to Amelanchier berries makes it possible to obtain a stable preserved product of high-quality, yielding moderate shrinkage and good retention of bioactive compounds. An optimized method of vacuum-microwave drying, applied directly to frozen fruit, allows the retention of 70 % of cyanidin glycosides present in the raw material.

Nutritional, microbial and physicochemical changes in pear juice under ultrasound and commercial pasteurization during storage

In this study, the microbial and nutritional aspects of pear juice with conventional or ultrasound (US) treatment during storage at 4 °C were studied. The conventional pasteurization was 95 °C for 5 min while US-pasteurizations (frequency 30 kHz, amplitude 70%) were 25, 45, and 65 °C for 10 min, under a probe sonicator (750 W). Control, US25 and US45 had shelf lives of 9, 12, and 15 days respectively. Ascorbic acid, total phenols, total anti oxidant capacity, and cloud value decreased in all treatments while pH, acidity, and sugars remained stable. Both treatments (P95 and US65) remained viable after 21 days of storage, however, loss of bioactive compounds in P95 (16.39%) was significantly higher than that of US65 (7.44%), indicating US-pasteurization a promising pear juice processing technology at low temperature, retaining bioactive compounds, meeting safety standards, and increasing shelf life. Practical applications Complete microbial and enzymatic deactivation is achieved at commercial thermal pasteurization techniques, but these elevated temperatures cause losses in nutritional and sensory attributes of the end product. While microbial and enzymatic deactivation in pear juice was achieved at lower temperature using thermosonication in the present study. The low temperature pasteurizations exhibited higher retention and availability of bioactive compounds than the traditional high temperature pasteurizations over the studied shelf life period. The findings of the study would pave way for the commercial processing of pear juice with improved quality and shelf life.

Bioactive compound retention in Echium amoenum Fisch. & C. A. Mey. petals: Effect of fluidized bed drying conditions

ABSTRACTThe dried petals of Echium amoenum Fisch. & C.A. Mey. are used for the treatment of many diseases but there are not any reports about the effects of drying conditions on their bioactive compounds. A complete 32 factorial design was used, where the independent variables were drying temperature (40°C–60°C) and air flow rate (0.5–1.0 m/s). The bioactive compounds studied were total phenolic, total flavonoid and anthocyanin contents, and antioxidant activity. The desirability index was used to predict the optimal drying conditions. Both independent variables were statistically significant. The optimal drying conditions were air velocity of 0.86 m/s at 60°C. The results show that the drying conditions play an important role in determining the final quality of the product, content of its bioactive compounds, and minimum drying time.

Short communication: Effects of vacuum freeze-drying on inactivation of Cronobacter sakazakii ATCC29544 in liquid media with different initial inoculum levels

Vacuum freeze-drying is an important food-processing technology for valid retention of nutrients and bioactive compounds. Cronobacter sakazakii has been reported to be associated with severe infections in neonates through consumption of contaminated powdered infant formula. In this study, effects of vacuum freeze-drying treatment for 12, 24, and 36 h on inactivation of C. sakazakii with different initial inoculum levels in sterile water, tryptic soy broth (TSB), skim milk, and whole milk were determined. Results indicated that the lethality rate of C. sakazakii in each sample increased with the extension of vacuum freeze-drying time. With initial inoculum levels of 102 and 103 cfu/mL, the survival of C. sakazakii in different liquid media was significantly affected by vacuum freeze-drying for 12, 24, and 36 h. In addition, the lethality rates of C. sakazakii in whole milk, skim milk, and TSB was significantly reduced compared with those in sterile water. Furthermore, whole milk showed the strongest protective role for C. sakazakii cells, followed by skim milk and TSB medium. Using the scanning electron microscope, the intracellular damage and obvious distortion of C. sakazakii cells were observed after vacuum freeze-drying for 24 and 36 h compared with the untreated sample, and the injured cells increased with the extension of vacuum-drying time. We concluded that inactivation of vacuum freeze-drying on C. sakazakii cells is related to the food matrix, and a combination with other methods for inactivating C. sakazakii is required for ensuring microbial safety of powdered infant formula.

Effects of Drying and Blanching on the Retention of Bioactive Compounds in Ginger and Turmeric

Ginger and turmeric, members of the Zingiberaceae family, are widely used for their pungent and aromatic flavour in foods and also for their medicinal properties. Both crops are often grown by smallholders in mountain areas on rich former forest soils with no need for fertilizers and pesticides, fulfilling de facto the conditions of organic agriculture. They are consumed fresh or dried. Drying is often performed without taking into account the content of bioactive compounds in the dried product. Various bioactive compounds have been identified in their rhizomes, and their content affects the price of the dried product. Hence, this study focused on the effects of drying treatments and blanching on the retention of bioactive compounds in the dried products. The bioactive compounds in ginger rhizome (Zingiber officinale Roscoe) are gingerols (particularly 6-gingerol). The drying treatments that were applied to fresh ginger included constant and also changing temperature conditions. Due to the short drying time, 60 °C was the optimal drying temperature to retain 6-gingerol. However, the changing temperature conditions significantly improved the retention of 6-gingerol. As for blanching, it had a significant negative effect on 6-gingerol retention. Turmeric (Curcuma longa) is known for its bright yellow colour and pharmacological properties due to curcumin, a phenolic compound. Drying was performed under constant conditions at 38 °C, 48 °C, 57 °C and 64 °C and a relative humidity of 20% and 40%. Drying at 57 °C with a lower relative humidity was the best drying treatment, yielding the highest amount of curcumin among non-blanched samples. Blanching for 15 min exhibited the highest curcumin yield while blanching for 5 min and 30 min did not have much effect. The findings of this study will benefit the industry in terms of improved quality control and cost reduction.

Engineering Process Characterization of High-Pressure Homogenization—from Laboratory to Industrial Scale

Fluid foods are a rapidly growing segment serving the needs of consumers’ healthy life style. Though high-pressure pasteurized beverages have been recently commercialized, the batch nature of the technology has been a hurdle for wider adaptation of the technology for high-throughput commodity-oriented beverage products. Further development of continuous flow through high-pressure processing methods is desired. High-pressure homogenization (HPH) consists of forcing a pressurized fluid to flow through a minute gap, which not only homogenizes the fluid but also increases the fluid’s temperature. HPH offers the possibility of combining homogenization and preservation into a single unit operation. By manipulating initial temperature and applied pressure, HPH treatment can be used to pasteurize or sterilize the product. This work critically examines the efforts in understanding fundamental process engineering aspects of HPH, including pressure-thermal process characterization, valve design (gap size and pressure relation), flow pattern, temperature history, and residence time distribution. This review will also highlight the HPH impact on food quality and bioactive compound retention. Quality aspects examined include particle size distribution and microbial and enzyme inactivation. The insight gained by this review will assist readers to gain a better appreciation of the HPH process design and system scale-up.

Osmotic dehydration of mango with impregnation of inulin and piquin-pepper oleoresin

This study assesses the effect of the osmotic dehydration (OD) of mango slices in an emulsion (600 g solids kg−1 emulsion) prepared with inulin and piquin-pepper oleoresin. In addition, mango was osmodehydrated in a sucrose solution for comparative purposes. The influence on water loss (WL) and solids gain (SG) during the OD process was analyzed. Furthermore, color change, oil gain, ascorbic acid, β-carotene, and total free phenolic content, as well as 1,1-diphenyl-2-picrylnydrazyl (DPPH) radical scavenging activity and the antiproliferative effect on breast cancer cell line MDA-MB-231 in mango slices after 120 min, were studied. Samples treated with the emulsion at 40 °C showed highest oil gain and bioactive compound retention. In addition, their ethanolic extract exhibited significant radical scavenging activity and antiproliferative effect against the cancer cell line tested, compared with that of flesh- and sucrose-treated sample extracts, in a dose-dependent manner. Images of mango slices treated in emulsion revealed the presence of inulin microcapsules with oil embedded in mango microstructure. Based on these results, this technique can be used to impregnate mango slices with oils and polymers with functional attributes to produce nutritious foods, which may serve as a potential source of phenolic with anticancer activity.

Fruit snacks from raspberries: influence of drying parameters on colour degradation and bioactive potential

AbstractRaspberries were dehydrated using air and freeze‐drying with wet and dry sugar infusion pretreatments. Product quality factors such as colour, bioactive compounds, antioxidant capacity and sensorial characteristics were analysed. Special emphasis was placed on the analysis of anthocyanin degradation and its relationship with colour deterioration and with polymeric compounds development and browning. Freeze‐dried raspberries presented a higher retention of bioactive compounds and a lower content of polymeric compounds than air‐dried ones. Dried samples without pretreatment (control) showed the highest retention of total phenolic content (freeze‐dried ≈82% and air‐dried ≈37% retention), but the lowest sensory acceptability. Although sugar infusion pretreatments caused an important loss of bioactive compounds (9–18% of TPC retention), a higher sensorial acceptability was obtained. Pretreatments with bisulphite and acid allowed obtaining the best quality attributes in terms of anthocyanin and polyphenol content, antiradical activity and colour retention. Polyphenol intake through pretreated dried raspberries (115–299 mg gallic ac./100 g intake) would be higher in some cases than that of usually consumed foods as vegetables, cereals and several fresh fruits.

Assessing the effect of traditional hulled wheat processing on bioactive compounds retention

Glume removal is the preliminary operation to make hulled wheats suitable to human consumption. Traditionally glumes are separated from kernels by means of stone mills, simultaneously causing kernel crushing, a loss of kernel parts and associated phytochemicals. The aim of this study was to compare bioactive compound retention of hulled wheat products obtained by traditional processing still used in Turkey and Armenia, with a more update plant located in Italy.On-plant samplings were carried out in two einkorn wheat bulgur processing plants in Turkey, and one emmer wheat processing plant, in Armenia and Italy. Whole and crushed kernels sampled at different processing stages were analysed for their phytochemical compound content. For the Italian plant, also the pearling process was considered.Whole kernels showed higher contents of lipid and phytochemical compounds than the correspondent processed fractions, with special respect for tocopherols and phytosterols. Carotenoid content was mainly affected by genotype.Pearling had a lower effect on the content of phytochemicals; however the correspondent waste fraction showed very high amounts of lipids and other compounds.All the plants showed similar retention of phytochemicals upon processing, with higher losses occurring in smaller kernel crushed fractions, mainly because of the loss of the germ.

Polydextrose as Wall Material for Microencapsulation of Yacon Juice by Spray Drying

The juice from yacon roots was encapsulated by spray drying using polydextrose and gum Arabic as wall materials. The effects of the concentration of the encapsulating agents and drying temperature on total phenolics, antioxidant activity, fructooligosaccharides, moisture content, water activity, solubility, hygroscopicity, color, and morphology of the microparticles were investigated to assess the potential use of polydextrose as wall material. The microparticles produced with polydextrose showed retention of bioactive compounds and physicochemical characteristics similar to those produced with gum Arabic. The phenolic retention ranged from 73.67 to 85.49 %, and the antioxidant activity by DPPH varied from 80.78 to 90.58 %. The fructooligosaccharides have undergone little depolymerization into simple sugars even at high temperatures. With respect to the physicochemical characteristics, high stability (low moisture and water activity), low hygroscopicity, and high solubility were observed in the microparticles. The spray dried samples showed a hue angle close to 100, indicating yellow color of the particles. Regarding the microstructure, particle agglomeration was observed in both treatments, probably due to the hygroscopic characteristic of the spray dried powders.

Effect of high pressure processing on carotenoid and phenolic compounds, antioxidant capacity, and microbial counts of bee-pollen paste and bee-pollen-based beverage

Abstract The optimal high pressure processing treatments (200–400 MPa, 5–15 min) of a pasty matrix of bee-pollen mixed with peptone water (1.5 g/mL) and bee-pollen added to a pineapple juice-based beverage matrix (0–10% (w/v)) were studied in order to guarantee food safety and maximum retention of bioactive compounds. Salmonella and yeasts were used as target microorganisms, while total carotenoid content (TCC), total phenolic content (TPC), and antioxidant capacity (FRAP) were studied from the food quality point of view. For the pasty matrix of bee-pollen, the results showed a significant influence of pressure and time, increasing the levels of TPC, FRAP, and TCC, in comparison with a control sample. A treatment of 395 MPa for 15 min was found as the optimal. For the pineapple juice-based beverage matrix, the factors pressure and bee-pollen concentration increased the levels of TPC, FRAP and TCC. Optimal conditions were found at 315 MPa for 14.5 min with 8% (w/v) of bee-pollen. Industrial relevance This investigation demonstrated the efficacy of the application of HPP on bee-pollen to inactivate microorganisms, both pathogenic and spoilage, up to 5-log 10 cycles. Additionally, a structural modification of the grain was achieved, with a consequent extractability of bioactive compounds and an increasing in the antioxidant activity, higher than 60% in comparison to fresh bee-pollen. The inclusion of bee-pollen in a fruit juice-based beverage matrix had a positive effect on the contribution of bioactive compounds that the fruit juice itself does not contain, such as carotenoids, for which bee-pollen can be considered as a natural additive that enhances the product functional characteristics.

Grape Juice Concentration by Progressive Freeze Concentrator Sequence System

Concentration is a complex process that could change the physical and chemical conditions of a substance and significantly affects the quality of fruit juice. Thus, this study aimed to evaluate the effect of different operating parameters on the bioactive compounds retention and the concentration effectiveness of grape juice by using progressive freeze concentration (PFC). The concentration process performance was assessed through total phenolic content (TPC) increment and effective partition constant (K). This research was carried out by using progressive freeze concentrator sequence system (PFCSS), which has been proven to be able to operate efficiently. It was found that the most optimum operating condition occurred with a coolant temperature of −8C, circulation flowrate of 3000 mL/min operation time of 20 minutes and solution concentration of 8%Brix. The results suggest that PFC may contribute a great potential in concentrating grape juice, therefore could contribute to the economy of the juice industry. Practical Applications Nowadays, there is a growing interest on progressive freeze concentration (PFC) in juice concentration due to its several advantages. In this study, a process sequence of PFC is develop to acquire high performance system in terms of operation time, no intervention of human operator and most importantly production of high quality product, which is grape juice concentrate. Red globe grape is among the most cultivated, most commercially important and studied cultivars, including processing method in order to produce high quality juice. This study is focused on the preservation and concentration of grape juice by using progressive freeze concentrator sequence system (PFCSS). The results can be a guide in the determination of processing method that can be used in producing red globe grape juice with good quality and highest concentration of TPC and other bioactive compound.

Retention of bioactive compounds in fresh fenugreek leaves during storage under modified atmosphere packaging

Purpose Fenugreek (Trigonella foenum-graecum) leaves have received an enormous demand due to their uses in traditional Indian preparations. It is a green leafy vegetable that is highly perishable in nature, due to high respiration rate; consequently, sensory as well as nutrient loss occurs at a very high rate. The aim of this research was to enhance the shelf life of fresh fenugreek leaves under modified atmosphere and determine the effect of different physiochemical parameters on storability of fenugreek leaves. Design/methodology/approach Fresh fenugreek leaves were stored in polypropylene (PP) film package under four different treatments, viz., T1: perforated (one perforation), T2: perforated (two perforation, 0.3 mm each), T3: non-perforated and T4: control sample. The control samples were kept in packages under ambient conditions. All packed samples were stored at 15°C temperature and 75 per cent relative humidity (RH). The quality was determined on the basis of retention of pigments, flavonoids, antioxidants, ascorbic acid and total phenols. The sensory evaluation of the stored samples was also carried out at regular intervals. Findings The results of this study indicated that the quality of fresh fenugreek leaves in PP (34 μm) non-perforated package (T3) stored at 15°C and 75 per cent RH was superior to those packed under other treatments on the basis of color retention, β-carotene, ascorbic acid, 2,2-diphenyl-1-picrylhydrazyl, total phenols and overall acceptability. The produce could be safely stored for 9 days without any adverse effect on bioactive compounds. Originality/value Factors leading to deterioration of quality of fresh fenugreek leaves like pigments and phenolic content were largely influenced by in-pack gaseous atmosphere inside the polymeric film packages. Modified atmosphere packaging with different treatments for enhancing shelf life and retention of bioactive compound of fenugreek was carried out in the work.